| 56 matches found in 25 documents. Search time: 0.011 seconds. |
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| Title: The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes .
| | Journal: Plant J Citation: V : 19 ( 1 ) P : 55-64 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10417726 Accession (PMID): 10417726 | | Abstract: Rice blast , caused by the fungal pathogen Magnaporthe grisea , is one of the most serious diseases of rice .
Here we describe the isolation and characterization of Pib , one of the rice blast resistance genes .
The Pib gene was isolated by a map-based cloning strategy .
The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site ( NBS ) and leucine-rich repeats ( LRRs ) ; thus , Pib is a member of the NBS-LRR class of plant disease resistance genes .
Interestingly , a duplication of the kinase 1a , 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein .
In addition , eight cysteine residues are clustered in the middle of the LRRs , a feature which has not been reported for other R genes .
Pib gene expression was induced upon altered environmental conditions , such as altered temperatures and darkness . | Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site ( NBS ) and leucine-rich repeats ( LRRs ) ; thus , Pib is a member of the NBS-LRR class of plant disease resistance genes .
[ Sen. 2, subscore: 1.00 ]: Here we describe the isolation and characterization of Pib , one of the rice blast resistance genes .
[ Sen. 3, subscore: 1.00 ]: The Pib gene was isolated by a map-based cloning strategy .
[ Sen. 5, subscore: 1.00 ]: Interestingly , a duplication of the kinase 1a , 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein .
[ Sen. 7, subscore: 1.00 ]: Pib gene expression was induced upon altered environmental conditions , such as altered temperatures and darkness .
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Score: 11.00 |
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| Title: Expression of the Pib rice-blast-resistance gene family is up-regulated by environmental conditions favouring infection and by chemical signals that trigger secondary plant defences .
| | Journal: Plant Mol . Biol . Citation: V : 47 ( 5 ) P : 653-61 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11725950 Accession (PMID): 11725950 | | Abstract: The rice blast resistance gene Pib is a member of the nucleotide binding site ( NBS ) and leucine-rich repeat ( LRR ) class of plant disease resistance ( R ) genes and belongs to a small gene family .
We describe here the isolation and characterization of a Pib homologue ( PibH8 ) , and extensive investigation of the expression of the Pib gene family ( Pib , PibH8 , HPibH8-1 , HPibH8-2 ) under various environmental and chemical treatments .
PibH8 shows 42% identity and 60% similarity to Pib and , like Pib , has a duplication of the kinase 1a , 2 , and 3a motifs of the NBS region in the N-terminal half of the protein .
Interestingly , genes of the Pib family exhibit a diurnal rhythm of expression .
RNA gel blot analysis revealed that their expression was regulated dramatically by environmental signals . such as temperature , light and water availability .
Their expression was also induced by chemical treatments , such as jasmonic acid , salicylic acid , ethylene and probenazole .
Our findings suggest that expression of the Pib gene family is up-regulated by environmental conditions that would favour pathogen infection .
This may reflect the evolution of anticipatory control of R gene expression . | Matching Sentences:
[ Sen. 2, subscore: 5.00 ]: We describe here the isolation and characterization of a Pib homologue ( PibH8 ) , and extensive investigation of the expression of the Pib gene family ( Pib , PibH8 , HPibH8-1 , HPibH8-2 ) under various environmental and chemical treatments .
[ Sen. 3, subscore: 3.00 ]: PibH8 shows 42% identity and 60% similarity to Pib and , like Pib , has a duplication of the kinase 1a , 2 , and 3a motifs of the NBS region in the N-terminal half of the protein .
[ Sen. 1, subscore: 1.00 ]: The rice blast resistance gene Pib is a member of the nucleotide binding site ( NBS ) and leucine-rich repeat ( LRR ) class of plant disease resistance ( R ) genes and belongs to a small gene family .
[ Sen. 4, subscore: 1.00 ]: Interestingly , genes of the Pib family exhibit a diurnal rhythm of expression .
[ Sen. 7, subscore: 1.00 ]: Our findings suggest that expression of the Pib gene family is up-regulated by environmental conditions that would favour pathogen infection .
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Score: 1.00 |
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| Title: Genetic control of rice blast resistance in the durably resistant cultivar Gumei 2 against multiple isolates .
| | Journal: Theor . Appl . Genet . Citation: V : 111 ( 1 ) P : 50-6 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15856160 Accession (PMID): 15856160 | | Abstract: To further our understanding of the genetic control of blast resistance in rice cultivar Gumei 2 and , consequently , to facilitate the utilization of this durably blast-resistant cultivar , we studied 304 recombinant inbred lines of indica rice cross Zhong 156/Gumei 2 and a linkage map comprising 181 markers .
An analysis of segregation for resistance against five isolates of rice blast suggested that one gene cluster and three additional major genes that are independently inherited are responsible for the complete resistance of Gumei 2 .
The gene cluster was located to chromosome 6 and includes two genes mapped previously , Pi25 ( t ) , against Chinese rice blast isolate 92-183 ( race ZC15 ) and Pi26 ( t ) against Philippine rice blast isolate Ca89 ( lineage 4 ) , and a gene for resistance against Philippine rice blast isolate 92330-5 ( lineage 17 ) .
Of the two genes conferring resistance against the Philippine isolates V86013 ( lineage 15 ) and C923-39 ( lineage 46 ) , we identified one as Pi26 ( t ) and mapped the other onto the distal end of chromosome 2 where Pib is located .
We used three components of partial blast resistance , percentage diseased leaf area ( DLA ) , lesion number and lesion size , all measured in the greenhouse , to measure the degree of susceptibility to isolates Ca89 and C923-39 and subsequently identified nine and eight quantitative trait loci ( QTLs ) , respectively .
Epistasis was determined to play an important role in partial resistance against Ca89 .
Using DLA measured on lines susceptible in a blast nursery , we detected six QTLs .
While different QTLs were detected for partial resistance to Ca89 and C923-39 , respectively , most were involved in the partial resistance in the field .
Our results suggest that the blast resistance in Gumei 2 is controlled by multiple major genes and minor genes with epistatic effects . | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: Of the two genes conferring resistance against the Philippine isolates V86013 ( lineage 15 ) and C923-39 ( lineage 46 ) , we identified one as Pi26 ( t ) and mapped the other onto the distal end of chromosome 2 where Pib is located .
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Score: 4.00 |
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| Title: Characterization of rice mutants with enhanced susceptibility to rice blast | | Journal: Mol .
Cells Citation: V : 20 ( 3 ) P : 385-91 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16404154 Accession (PMID): 16404154 | | Abstract: As a first step towards identifying genes involving in the signal transduction pathways mediating rice blast resistance , we isolated 3 mutants lines that showed enhanced susceptibility to rice blast KJ105 ( 91-033 ) from a T-DNA insertion library of the japonica rice cultivar , Hwayeong .
Since none of the susceptible phenotypes co-segregated with the T-DNA insertion we adapted a map-based cloning strategy to isolate the gene ( s ) responsible for the enhanced susceptibility of the Hwayeong mutants .
A genetic mapping population was produced by crossing the resistant wild type Hwayeong with the susceptible cultivar , Nagdong .
Chi-square analysis of the F2 segregating population indicated that resistance in Hwayeong was controlled by a single major gene that we tentatively named Pi-hy .
Randomly selected susceptible plants in the F2 population were used to build an initial map of Pi-hy .
The SSLP marker RM2265 on chromosome 2 was closely linked to resistance .
High resolution mapping using 105 F2 plants revealed that the resistance gene was tightly linked , or identical , to Pib , a resistance gene with a nucleotide binding sequence and leucine-rich repeats ( NB-LRR ) previously isolated .
Sequence analysis of the Pib locus amplified from three susceptible mutants revealed lesions within this gene , demonstrating that the Pi-hy gene is Pib .
The Pib mutations in 1D-22-10-13 , 1D-54-16-8 , and 1C-143-16-1 were , respectively , a missense mutation in the conserved NB domain 3 , a nonsense mutation in the 5th LRR , and a nonsense mutation in the C terminus following the LRRs that causes a small deletion of the C terminus .
These findings provide evidence that NB domain 3 and the C terminus are required for full activity of the plant R gene .
They also suggest that alterations of the resistance gene can cause major differences in pathogen specificity by affecting interactions with an avirulence factor . | Matching Sentences:
[ Sen. 8, subscore: 2.00 ]: Sequence analysis of the Pib locus amplified from three susceptible mutants revealed lesions within this gene , demonstrating that the Pi-hy gene is Pib .
[ Sen. 7, subscore: 1.00 ]: High resolution mapping using 105 F2 plants revealed that the resistance gene was tightly linked , or identical , to Pib , a resistance gene with a nucleotide binding sequence and leucine-rich repeats ( NB-LRR ) previously isolated .
[ Sen. 9, subscore: 1.00 ]: The Pib mutations in 1D-22-10-13 , 1D-54-16-8 , and 1C-143-16-1 were , respectively , a missense mutation in the conserved NB domain 3 , a nonsense mutation in the 5th LRR , and a nonsense mutation in the C terminus following the LRRs that causes a small deletion of the C terminus .
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Score: 1.00 |
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| Title: Development of PCR-based allele-specific and InDel marker sets for nine rice blast resistance genes .
| | Journal: Citation: V : ( ) P : Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16673143 Accession (PMID): 16673143 | | Abstract: Blast resistance is one of the most important traits in rice breeding , and application of molecular markers for blast resistance breeding is likely to allow the rapid screening for the trait during early growth stages , without the need for inoculation of pathogen and phenotyping .
Allele-specific PCR markers and insertion/deletion ( InDel ) markers , which genotype single-nucleotide polymorphisms and InDel polymorphisms , respectively , are useful tools for marker-assisted selections .
We developed sets of allele-specific PCR and InDel markers for nine rice blast resistance genes-Piz , Piz-t , Pit , Pik , Pik-m , Pik-p , Pita , Pita-2 , and Pib-which are commonly used in Japanese blast resistance rice breeding programs .
For each resistance gene , we used the segregation information from thousands of progeny in several crosses or published gene locations to generate a marker that cosegregated with the gene and markers that closely flanked the gene on either side .
The developed cosegregating markers uniquely discriminated among each of the lines with the individual resistance genes ( except for Pita and Pita-2 ) .
Therefore , these markers will likely facilitate the development of multiline cultivars carrying one or a combination of these nine blast resistance genes .
In addition , the systems we developed may be valuable tools in the quality control of seed production from blast-resistant multiline cultivars .
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[ Sen. 3, subscore: 1.00 ]: We developed sets of allele-specific PCR and InDel markers for nine rice blast resistance genes-Piz , Piz-t , Pit , Pik , Pik-m , Pik-p , Pita , Pita-2 , and Pib-which are commonly used in Japanese blast resistance rice breeding programs .
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Score: 1.00 |
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| Title: Development of PCR-based allele-specific and InDel marker sets for nine rice blast resistance genes .
| | Journal: Theor . Appl . Genet . Citation: V : 113 ( 2 ) P : 251-60 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16791691 Accession (PMID): 16791691 | | Abstract: Blast resistance is one of the most important traits in rice breeding , and application of molecular markers for blast resistance breeding is likely to allow the rapid screening for the trait during early growth stages , without the need for inoculation of pathogen and phenotyping .
Allele-specific PCR markers and insertion/deletion ( InDel ) markers , which genotype single-nucleotide polymorphisms and InDel polymorphisms , respectively , are useful tools for marker-assisted selections .
We developed sets of allele-specific PCR and InDel markers for nine rice blast resistance genes -- Piz , Piz-t , Pit , Pik , Pik-m , Pik-p , Pita , Pita-2 , and Pib -- which are commonly used in Japanese blast resistance rice breeding programs .
For each resistance gene , we used the segregation information from thousands of progeny in several crosses or published gene locations to generate a marker that cosegregated with the gene and markers that closely flanked the gene on either side .
The developed cosegregating markers uniquely discriminated among each of the lines with the individual resistance genes ( except for Pita and Pita-2 ) .
Therefore , these markers will likely facilitate the development of multiline cultivars carrying one or a combination of these nine blast resistance genes .
In addition , the systems we developed may be valuable tools in the quality control of seed production from blast-resistant multiline cultivars . | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: We developed sets of allele-specific PCR and InDel markers for nine rice blast resistance genes -- Piz , Piz-t , Pit , Pik , Pik-m , Pik-p , Pita , Pita-2 , and Pib -- which are commonly used in Japanese blast resistance rice breeding programs .
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Score: 5.00 |
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| Title: [ The inductive activation of the promoter of pib gene ] | | Journal: Yi Chuan Citation: V : 28 ( 6 ) P : 689-94 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16818431 Accession (PMID): 16818431 | | Abstract: A 5 . 7 kb putative promoter region of pib gene was isolated from the pib genomic clone and substituted for the 35S promoter upstream of gus gene in plasmid pCAMBIA1301 to construct a new plant expression vector pNAR604 ( putative pib promoter-GUS + 35S-hpt ) .
From Agrobacterium-mediated transformation and hygromycin selective culture in vitro , hygromycin resistant calli and 36 transgenic rice ( Oryza sativa L ) plants were obtained .
Histochemical assays of GUS activity showed that no expression was observed in the resistant calli and roots from transgenic rice if cultured under light , but after 24 h dark treatment there was strong GUS staining .
Fluorimetric quantitative analysis indicated that GUS expression was organ-specific in transgenic rice .
Without the dark treatment , GUS activity in roots and stems were about 7 and 3 times higher than in leaves in which GUS activity was only trace detected .
After 24 h dark treatment , GUS activity in roots , stems and leaves of transgenic plants were all promoted and the largest increase was observed in leaves .
Twenty-four hours after spraying with 5 mmol/L SA ( Salicylic Acid ) or 0 . 3 mol/L NaCl , GUS activity in leaves of the transgenic plants was 2 . 7 or 3 . 6 times respectively higher than untreated control .
It was confirmed that an inductive promoter was involved in this 5 . 7 kb upstream region of pib gene , and dark , SA and NaCl treatments were inductive factors for pib promoter . | Matching Sentences:
[ Sen. 1, subscore: 3.00 ]: A 5 . 7 kb putative promoter region of pib gene was isolated from the pib genomic clone and substituted for the 35S promoter upstream of gus gene in plasmid pCAMBIA1301 to construct a new plant expression vector pNAR604 ( putative pib promoter-GUS + 35S-hpt ) .
[ Sen. 8, subscore: 2.00 ]: It was confirmed that an inductive promoter was involved in this 5 . 7 kb upstream region of pib gene , and dark , SA and NaCl treatments were inductive factors for pib promoter .
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Score: 1.00 |
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| Title: The in silico map-based cloning of Pi36 , a rice coiled-coil nucleotide-binding site leucine-rich repeat gene that confers race-specific resistance to the blast fungus .
| | Journal: Genetics Citation: V : 176 P : Sep-41 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17507669 Accession (PMID): 17507669 | | Abstract: The indica rice variety Kasalath carries Pi36 , a gene that determines resistance to Chinese isolates of rice blast and that has been located to a 17-kb interval on chromosome 8 .
The genomic sequence of the reference japonica variety Nipponbare was used for an in silico prediction of the resistance ( R ) gene content of the interval and hence for the identification of candidate gene ( s ) for Pi36 .
Three such sequences , which all had both a nucleotide-binding site and a leucine-rich repeat motif , were present .
The three candidate genes were amplified from the genomic DNA of a number of varieties by long-range PCR , and the resulting amplicons were inserted into pCAMBIA1300 and/or pYLTAC27 vectors to determine sequence polymorphisms correlated to the resistance phenotype and to perform transgenic complementation tests .
Constructs containing each candidate gene were transformed into the blast-susceptible variety Q1063 , which allowed the identification of Pi36-3 as the functional gene , with the other two candidates being probable pseudogenes .
The Pi36-encoded protein is composed of 1056 amino acids , with a single substitution event ( Asp to Ser ) at residue 590 associated with the resistant phenotype .
Pi36 is a single-copy gene in rice and is more closely related to the barley powdery mildew resistance genes Mla1 and Mla6 than to the rice blast R genes Pita , Pib , Pi9 , and Piz-t .
An RT-PCR analysis showed that Pi36 is constitutively expressed in Kasalath .
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[ Sen. 7, subscore: 1.00 ]: Pi36 is a single-copy gene in rice and is more closely related to the barley powdery mildew resistance genes Mla1 and Mla6 than to the rice blast R genes Pita , Pib , Pi9 , and Piz-t .
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Score: 1.00 |
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| Title: The blast resistance gene Pi37 encodes a nucleotide binding site leucine-rich repeat protein and is a member of a resistance gene cluster on rice chromosome 1 .
| | Journal: Genetics Citation: V : 177 P : 1871-80 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17947408 Accession (PMID): 17947408 | | Abstract: The resistance ( R ) gene Pi37 , present in the rice cultivar St No 1 , was isolated by an in silico map-based cloning procedure .
The equivalent genetic region in Nipponbare contains four nucleotide binding site-leucine-rich repeat ( NBS-LRR ) type loci .
These four candidates for Pi37 ( Pi37-1 , -2 , -3 , and -4 ) were amplified separately from St No 1 via long-range PCR , and cloned into a binary vector .
Each construct was individually transformed into the highly blast susceptible cultivar Q1063 .
The subsequent complementation analysis revealed Pi37-3 to be the functional gene , while -1 , -2 , and -4 are probably pseudogenes .
Pi37 encodes a 1290 peptide NBS-LRR product , and the presence of substitutions at two sites in the NBS region ( V239A and I247M ) is associated with the resistance phenotype .
Semiquantitative expression analysis showed that in St No 1 , Pi37 was constitutively expressed and only slightly induced by blast infection .
Transient expression experiments indicated that the Pi37 product is restricted to the cytoplasm .
Pi37-3 is thought to have evolved recently from -2 , which in turn was derived from an ancestral -1 sequence .
Pi37-4 is likely the most recently evolved member of the cluster and probably represents a duplication of -3 .
The four Pi37 paralogs are more closely related to maize rp1 than to any of the currently isolated rice blast R genes Pita , Pib , Pi9 , Pi2 , Piz-t , and Pi36 .
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[ Sen. 11, subscore: 1.00 ]: The four Pi37 paralogs are more closely related to maize rp1 than to any of the currently isolated rice blast R genes Pita , Pib , Pi9 , Pi2 , Piz-t , and Pi36 .
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Score: 3.00 |
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| Title: [ Initial functional analysis of the promoter region and coding region of Pib gene in transgenic rice ] | | Journal: Yi Chuan Citation: V : 30 P : 367-72 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub18332008 Accession (PMID): 18332008 | | Abstract: The promoter region and intact coding region of Pib gene ( 9 . 9 kb ) was inserted into the downstream of CaMV 35S promoter in a binary vector pPZP2Ha3 ( + ) , resulting a plasmid pNAR701 .
And a fragment of Pib gene from 6 986 to 9 392 bp was placed into pPZP2Ha3 ( - ) under the control of CaMV 35S promoter , producing an antisense expression vector pNAR703 .
These two recombined vectors were transferred into a blast medium susceptible rice cultivar R109 by an Agrobecterium-mediated method .
Tests of PCR and Southern blotting for transgenic plants as well as the segregation of hygromycin resistance in T1 generation confirmed that the target DNA fragments were integrated into genome of R109 and hereditable .
Northern blotting analysis showed the coding region of Pib gene double driven by 35S and its native promoter was able to transcript in T1 transgenic plants .
Rice blast resistance test for T1 transgenic seedlings of 3-4 leaves stage and in vitro leaves in tillering stage showed that transgenic plants of pNAR701 were more resistant to blast race ZD1 and ZG1 than the wild type plants , but the resistance of antisense transgenic plants from pNAR703 was decreased compared to the controls .
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[ Sen. 1, subscore: 1.00 ]: The promoter region and intact coding region of Pib gene ( 9 . 9 kb ) was inserted into the downstream of CaMV 35S promoter in a binary vector pPZP2Ha3 ( + ) , resulting a plasmid pNAR701 .
[ Sen. 2, subscore: 1.00 ]: And a fragment of Pib gene from 6 986 to 9 392 bp was placed into pPZP2Ha3 ( - ) under the control of CaMV 35S promoter , producing an antisense expression vector pNAR703 .
[ Sen. 5, subscore: 1.00 ]: Northern blotting analysis showed the coding region of Pib gene double driven by 35S and its native promoter was able to transcript in T1 transgenic plants .
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