| 35 matches found in 15 documents. Search time: 0.402 seconds. |
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| Title: Inheritance and molecular mapping of two fertility-restoring loci for Honglian gametophytic cytoplasmic male sterility in rice ( Oryza sativaL . ) .
| | Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15057557 Accession (PMID): 15057557 | | Abstract: The Honglian cytoplasmic male sterility ( cms-HL ) system , a novel type of gametophytic CMS in indica rice , is being used for the large-scale commercial production of hybrid rice in China .
However , the genetic basis of fertility restoration ( Rf ) in cms-HL remains unknown .
Previous studies have shown that fertility restoration is controlled by a single locus located on chromosome 10 , close to the loci Rf1 and Rf4 , which respond to cms-BT and cms-WA , respectively .
To determine if the Rf locus for cms-HL is different from these Rf loci and to establish fine-scale genetic and physical maps for map-based cloning of the Rf gene , high-resolution mapping of the Rf gene was carried out using RAPD and microsatellite markers in three BCF ( 1 ) populations .
The results of the genetic linkage analysis indicated that two Rf loci respond to cms-HL , and that these are located in different regions of chromosome 10 .
One of these loci , Rf5 , co-segregates with the SSR marker RM3150 , and is flanked by RM1108 and RM5373 , which are 0 . 9 cM and 1 . 3 cM away , respectively .
Another Rf locus , designated as Rf6 ( t ) , co-segregates with RM5373 , and is flanked by RM6737 and SBD07 at genetic distances of 0 . 4 cM .
The results also demonstrated these loci are distinct from Rf1 and Rf4 .
A 105-kb BAC clone covering the Rf6 ( t ) locus was obtained from a rice BAC library .
The sequence of a 66-kb segment spanning the Rf6 ( t ) locus was determined by a BLASTX search in the genomic sequence database established for the cultivar 93-11 . | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: Previous studies have shown that fertility restoration is controlled by a single locus located on chromosome 10 , close to the loci Rf1 and Rf4 , which respond to cms-BT and cms-WA , respectively .
[ Sen. 8, subscore: 1.00 ]: The results also demonstrated these loci are distinct from Rf1 and Rf4 .
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Score: 4.00 |
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| Title: Fertility restorer locus Rf1 [ corrected ] of sorghum ( Sorghum bicolor L ) encodes a pentatricopeptide repeat protein not present in the colinear region of rice chromosome 12 .
| | Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16078015 Accession (PMID): 16078015 | | Abstract: With an aim to clone the sorghum fertility restorer gene Rf1 , a high-resolution genetic and physical map of the locus was constructed .
The Rf1 locus was resolved to a 32-kb region spanning four open reading frames : a plasma membrane Ca ( 2+ ) -ATPase , a cyclin D-1 , an unknown protein , and a pentatricopeptide repeat ( PPR13 ) gene family member .
An approximately 19-kb region spanning the cyclin D-1 and unknown protein genes was completely conserved between sterile and fertile plants as was the sequence spanning the coding region of the Ca ( 2+ ) -ATPase .
In contrast , 19 sequence polymorphisms were located in an approximately 7-kb region spanning PPR13 , and all markers cosegregated with the fertility restoration phenotype .
PPR13 was predicted to encode a mitochondrial-targeted protein containing a single exon with 14 PPR repeats , and the protein is classified as an E-type PPR subfamily member .
To permit sequence-based comparison of the sorghum and rice genomes in the Rf1 region , 0 . 53 Mb of sorghum chromosome 8 was sequenced and compared to the colinear region of rice chromosome 12 .
Genome comparison revealed a mosaic pattern of colinearity with an approximately 275-kb gene-poor region with little gene conservation and an adjacent , approximately 245-kb gene-rice region that is more highly conserved between rice and sorghum .
Despite being located in a region of high gene conservation , sorghum PPR13 was not located in a colinear position on rice chromosome 12 .
The present results suggest that sorghum PPR13 represents a potential candidate for the sorghum Rf1 gene , and its presence in the sorghum genome indicates a single gene transposition event subsequent to the divergence of rice and sorghum ancestors . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: With an aim to clone the sorghum fertility restorer gene Rf1 , a high-resolution genetic and physical map of the locus was constructed .
[ Sen. 2, subscore: 1.00 ]: The Rf1 locus was resolved to a 32-kb region spanning four open reading frames : a plasma membrane Ca ( 2+ ) -ATPase , a cyclin D-1 , an unknown protein , and a pentatricopeptide repeat ( PPR13 ) gene family member .
[ Sen. 6, subscore: 1.00 ]: To permit sequence-based comparison of the sorghum and rice genomes in the Rf1 region , 0 . 53 Mb of sorghum chromosome 8 was sequenced and compared to the colinear region of rice chromosome 12 .
[ Sen. 9, subscore: 1.00 ]: The present results suggest that sorghum PPR13 represents a potential candidate for the sorghum Rf1 gene , and its presence in the sorghum genome indicates a single gene transposition event subsequent to the divergence of rice and sorghum ancestors .
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Score: 9.00 |
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| Title: Cytoplasmic male sterility of rice with boro II cytoplasm is caused by a cytotoxic peptide and is restored by two related PPR motif genes via distinct modes of mRNA silencing .
| | Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16489123 Accession (PMID): 16489123 | | Abstract: Cytoplasmic male sterility ( CMS ) and nucleus-controlled fertility restoration are widespread plant reproductive features that provide useful tools to exploit heterosis in crops .
However , the molecular mechanism underlying this kind of cytoplasmic-nuclear interaction remains unclear .
Here , we show in rice ( Oryza sativa ) with Boro II cytoplasm that an abnormal mitochondrial open reading frame , orf79 , is cotranscribed with a duplicated atp6 ( B-atp6 ) gene and encodes a cytotoxic peptide .
Expression of orf79 in CMS lines and transgenic rice plants caused gametophytic male sterility .
Immunoblot analysis showed that the ORF79 protein accumulates specifically in microspores .
Two fertility restorer genes , Rf1a and Rf1b , were identified at the classical locus Rf-1 as members of a multigene cluster that encode pentatricopeptide repeat proteins .
RF1A and RF1B are both targeted to mitochondria and can restore male fertility by blocking ORF79 production via endonucleolytic cleavage ( RF1A ) or degradation ( RF1B ) of dicistronic B-atp6/orf79 mRNA .
In the presence of both restorers , RF1A was epistatic over RF1B in the mRNA processing .
We have also shown that RF1A plays an additional role in promoting the editing of atp6 mRNAs , independent of its cleavage function . | Matching Sentences:
[ Sen. 7, subscore: 4.00 ]: RF1A and RF1B are both targeted to mitochondria and can restore male fertility by blocking ORF79 production via endonucleolytic cleavage ( RF1A ) or degradation ( RF1B ) of dicistronic B-atp6/orf79 mRNA .
[ Sen. 6, subscore: 2.00 ]: Two fertility restorer genes , Rf1a and Rf1b , were identified at the classical locus Rf-1 as members of a multigene cluster that encode pentatricopeptide repeat proteins .
[ Sen. 8, subscore: 2.00 ]: In the presence of both restorers , RF1A was epistatic over RF1B in the mRNA processing .
[ Sen. 9, subscore: 1.00 ]: We have also shown that RF1A plays an additional role in promoting the editing of atp6 mRNAs , independent of its cleavage function .
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Score: 8.00 |
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| Title: Retrograde regulation of nuclear gene expression in CW-CMS of rice .
| | Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub17086445 Accession (PMID): 17086445 | | Abstract: The CW-cytoplasmic male sterility ( CMS ) line has the cytoplasm of Oryza rufipogon Griff , and mature pollen is morphologically normal under an optical microscope but lacks the ability to germinate ; restorer gene Rf17 has been identified as restoring this ability .
The difference between nuclear gene expression in mature anthers was compared for the CW-CMS line , [ cms-CW ] rf17rf17 , and a maintainer line with normal cytoplasm of Oryza sativa L , [ normal ] rf17rf17 .
Using a 22-k rice oligoarray we detected 58 genes that were up-regulated more than threefold in the CW-CMS line .
Expression in other organs was further investigated for 20 genes using RT-PCR .
Five genes , including genes for alternative oxidase , were found to be preferentially expressed in [ cms-CW ] rf17rf17 but not in [ normal ] rf17rf17 or [ cms-CW ] Rf17Rf17 .
Such [ cms-CW ] rf17rf17-specific gene expression was only observed in mature anthers but not in leaves , stems , or roots , indicating the presence of anther-specific mitochondrial retrograde regulation of nuclear gene expression , and that Rf17 has a role in restoring the ectopic gene expression .
We also used a proteomic approach to discover the retrograde regulated proteins and identified six proteins that were accumulated differently .
These results reveal organ-specific induced mitochondrial retrograde pathways affecting nuclear gene expression possibly related to CMS . | Matching Sentences:
[ Sen. 5, subscore: 3.00 ]: Five genes , including genes for alternative oxidase , were found to be preferentially expressed in [ cms-CW ] rf17rf17 but not in [ normal ] rf17rf17 or [ cms-CW ] Rf17Rf17 .
[ Sen. 2, subscore: 2.00 ]: The difference between nuclear gene expression in mature anthers was compared for the CW-CMS line , [ cms-CW ] rf17rf17 , and a maintainer line with normal cytoplasm of Oryza sativa L , [ normal ] rf17rf17 .
[ Sen. 6, subscore: 2.00 ]: Such [ cms-CW ] rf17rf17-specific gene expression was only observed in mature anthers but not in leaves , stems , or roots , indicating the presence of anther-specific mitochondrial retrograde regulation of nuclear gene expression , and that Rf17 has a role in restoring the ectopic gene expression .
[ Sen. 1, subscore: 1.00 ]: The CW-cytoplasmic male sterility ( CMS ) line has the cytoplasm of Oryza rufipogon Griff , and mature pollen is morphologically normal under an optical microscope but lacks the ability to germinate ; restorer gene Rf17 has been identified as restoring this ability .
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Score: 2.00 |
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| Title: Suppression mechanism of mitochondrial ORF79 accumulation by Rf1 protein in BT-type cytoplasmic male sterile rice .
| | Year: 2008 Type: Publisher | | Literature: oryza Field: abstract Doc ID: pub18435825 Accession (PMID): 18435825 | | Abstract: In BT-type cytoplasmic male sterile rice ( Oryza sativa L ) with Chinsurah Boro II cytoplasm , cytoplasmic male sterility ( CMS ) is caused by an accumulation of the cytotoxic peptide ORF79 .
The ORF79 protein is expressed from a dicistronic gene atp6-orf79 , which exists in addition to the normal atp6 gene in the BT-type mitochondrial genome .
The CMS is restored by a PPR ( pentatricopeptide-repeat ) gene , Rf1 , via RNA processing .
However , it has not yet been elucidated how the accumulation of ORF79 is reduced by the action of the Rf1 protein .
Here , we report that the level of processed orf79 transcripts in the restorer line was reduced to 50% of the unprocessed atp6-orf79 transcripts in the CMS line .
Ninety percent of the processed orf79 transcripts , which remained after degradation , were not associated with the ribosome for translation .
Our data suggests that the processing of atp6-orf79 transcripts diminishes the expression of orf79 by the translational reduction and degradation of the processed orf79 transcripts .
| Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: The CMS is restored by a PPR ( pentatricopeptide-repeat ) gene , Rf1 , via RNA processing .
[ Sen. 4, subscore: 1.00 ]: However , it has not yet been elucidated how the accumulation of ORF79 is reduced by the action of the Rf1 protein .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
