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Score: 6.00 |
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| Title: In vivo and in vitro phosphorylation of rice dwarf phytoreovirus Pns12 cytoplasmic nonstructural protein .
| | Author: Suzuki N Hosokawa D Matsuura Y Kikuchi A Omura T | | Journal: Arch . Virol . Citation: V : 144 ( 7 ) P : 1371-80 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10481743 | | Abstract: In vivo and in vitro phosphorylation and intracellular location of rice dwarf phytoreovirus Pns12 , which is encoded by one of the twelve dsRNA genome segments , S12 , and comprises 312 amino acids , was investigated .
When [ 32P ] phosphoric acid was incorporated into RDV-infected leafhopper cultured cells , labelled Pns12 was immunoprecipitated from the cells by a monospecific anti-Pns12 polyclonal antibody .
Recombinant Pns12 was purified from Spodoptera frugiperda cells infected with AcRS12 , a baculovirus recombinant carrying a full-length cDNA of RDV S12 .
Purified Pns12 was also demonstrated to be phosphorylated in vitro by a kinase activity present in host ( rice , barley , wheat , leafhopper ) cells and non-host ( tobacco , spinach , white clover , S frugiperda , mosquito , mammals ) cells as well .
Immunocytochemical studies showed Pns12 accumulated in the cytoplasm of infected cells , and frequently localized in a slightly electron-dense patch .
These results demonstrated that RDV Pns12 was a cytoplasmic nonstructural phosphoprotein . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: In vivo and in vitro phosphorylation and intracellular location of rice dwarf phytoreovirus Pns12 , which is encoded by one of the twelve dsRNA genome segments , S12 , and comprises 312 amino acids , was investigated . When [ 32P ] phosphoric acid was incorporated into RDV-infected leafhopper cultured cells , labelled Pns12 was immunoprecipitated from the cells by a monospecific anti-Pns12 polyclonal antibody . Recombinant Pns12 was purified from Spodoptera frugiperda cells infected with AcRS12 , a baculovirus recombinant carrying a full-length cDNA of RDV S12 . Purified Pns12 was also demonstrated to be phosphorylated in vitro by a kinase activity present in host ( rice , barley , wheat , leafhopper ) cells and non-host ( tobacco , spinach , white clover , S frugiperda , mosquito , mammals ) cells as well . Immunocytochemical studies showed Pns12 accumulated in the cytoplasm of infected cells , and frequently localized in a slightly electron-dense patch .
[ Sen. 2, subscore: 1.00 ]: In vivo and in vitro phosphorylation and intracellular location of rice dwarf phytoreovirus Pns12 , which is encoded by one of the twelve dsRNA genome segments , S12 , and comprises 312 amino acids , was investigated . When [ 32P ] phosphoric acid was incorporated into RDV-infected leafhopper cultured cells , labelled Pns12 was immunoprecipitated from the cells by a monospecific anti-Pns12 polyclonal antibody . Recombinant Pns12 was purified from Spodoptera frugiperda cells infected with AcRS12 , a baculovirus recombinant carrying a full-length cDNA of RDV S12 . Purified Pns12 was also demonstrated to be phosphorylated in vitro by a kinase activity present in host ( rice , barley , wheat , leafhopper ) cells and non-host ( tobacco , spinach , white clover , S frugiperda , mosquito , mammals ) cells as well . Immunocytochemical studies showed Pns12 accumulated in the cytoplasm of infected cells , and frequently localized in a slightly electron-dense patch . These results demonstrated that RDV Pns12 was a cytoplasmic nonstructural phosphoprotein .
[ Sen. 3, subscore: 1.00 ]: In vivo and in vitro phosphorylation and intracellular location of rice dwarf phytoreovirus Pns12 , which is encoded by one of the twelve dsRNA genome segments , S12 , and comprises 312 amino acids , was investigated . When [ 32P ] phosphoric acid was incorporated into RDV-infected leafhopper cultured cells , labelled Pns12 was immunoprecipitated from the cells by a monospecific anti-Pns12 polyclonal antibody . Recombinant Pns12 was purified from Spodoptera frugiperda cells infected with AcRS12 , a baculovirus recombinant carrying a full-length cDNA of RDV S12 . Purified Pns12 was also demonstrated to be phosphorylated in vitro by a kinase activity present in host ( rice , barley , wheat , leafhopper ) cells and non-host ( tobacco , spinach , white clover , S frugiperda , mosquito , mammals ) cells as well . Immunocytochemical studies showed Pns12 accumulated in the cytoplasm of infected cells , and frequently localized in a slightly electron-dense patch . These results demonstrated that RDV Pns12 was a cytoplasmic nonstructural phosphoprotein .
[ Sen. 4, subscore: 1.00 ]: In vivo and in vitro phosphorylation and intracellular location of rice dwarf phytoreovirus Pns12 , which is encoded by one of the twelve dsRNA genome segments , S12 , and comprises 312 amino acids , was investigated . When [ 32P ] phosphoric acid was incorporated into RDV-infected leafhopper cultured cells , labelled Pns12 was immunoprecipitated from the cells by a monospecific anti-Pns12 polyclonal antibody . Recombinant Pns12 was purified from Spodoptera frugiperda cells infected with AcRS12 , a baculovirus recombinant carrying a full-length cDNA of RDV S12 . Purified Pns12 was also demonstrated to be phosphorylated in vitro by a kinase activity present in host ( rice , barley , wheat , leafhopper ) cells and non-host ( tobacco , spinach , white clover , S frugiperda , mosquito , mammals ) cells as well . Immunocytochemical studies showed Pns12 accumulated in the cytoplasm of infected cells , and frequently localized in a slightly electron-dense patch . These results demonstrated that RDV Pns12 was a cytoplasmic nonstructural phosphoprotein .
[ Sen. 5, subscore: 1.00 ]: In vivo and in vitro phosphorylation and intracellular location of rice dwarf phytoreovirus Pns12 , which is encoded by one of the twelve dsRNA genome segments , S12 , and comprises 312 amino acids , was investigated . When [ 32P ] phosphoric acid was incorporated into RDV-infected leafhopper cultured cells , labelled Pns12 was immunoprecipitated from the cells by a monospecific anti-Pns12 polyclonal antibody . Recombinant Pns12 was purified from Spodoptera frugiperda cells infected with AcRS12 , a baculovirus recombinant carrying a full-length cDNA of RDV S12 . Purified Pns12 was also demonstrated to be phosphorylated in vitro by a kinase activity present in host ( rice , barley , wheat , leafhopper ) cells and non-host ( tobacco , spinach , white clover , S frugiperda , mosquito , mammals ) cells as well . Immunocytochemical studies showed Pns12 accumulated in the cytoplasm of infected cells , and frequently localized in a slightly electron-dense patch . These results demonstrated that RDV Pns12 was a cytoplasmic nonstructural phosphoprotein .
[ Sen. 6, subscore: 1.00 ]: When [ 32P ] phosphoric acid was incorporated into RDV-infected leafhopper cultured cells , labelled Pns12 was immunoprecipitated from the cells by a monospecific anti-Pns12 polyclonal antibody . Recombinant Pns12 was purified from Spodoptera frugiperda cells infected with AcRS12 , a baculovirus recombinant carrying a full-length cDNA of RDV S12 . Purified Pns12 was also demonstrated to be phosphorylated in vitro by a kinase activity present in host ( rice , barley , wheat , leafhopper ) cells and non-host ( tobacco , spinach , white clover , S frugiperda , mosquito , mammals ) cells as well . Immunocytochemical studies showed Pns12 accumulated in the cytoplasm of infected cells , and frequently localized in a slightly electron-dense patch . These results demonstrated that RDV Pns12 was a cytoplasmic nonstructural phosphoprotein .
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Score: 3.00 |
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| Title: Glutelin basic subunits have a mammalian mucin-type O-linked disaccharide side chain .
| | Author: Kishimoto T Watanabe M Mitsui T Hori H | | Journal: Arch . Biochem . Biophys . Citation: V : 370 ( 2 ) P : 271-7 Year: 1999 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10510286 | | Abstract: Characterization was done on the sugar chains of glutelin , a major storage protein in rice seeds .
The basic subunits of glutelin were shown to be glycoproteins by staining with PAS and the lectins concanavalin A ( Con A ) and peanut agglutinin ( PNA ) .
The binding of PNA to the basic subunits was substantially reduced by treatment of the glutelin with NaIO4 , NaOH , or O-glycanase .
The sugar chains of the subunits , obtained by hydrazinolysis and O-glycanase , were pyridylaminated and subjected to 2-dimensional HPLC analysis using C ( 18 ) and acrylamide-derivatized columns .
It was found that the Galbeta-1 , 3GalNAc disaccharide , which was previously identified as a core 1 structure of mucin-type sugar chains , is conjugated to the glutelin subunits .
Furthermore , amino acid sequencing of an 11-kDa peptide of the subunits , recognized by both PNA and Con A , suggested that both N-linked and O-linked glycosylation occurs in the carboxy-terminal region of these subunits . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Characterization was done on the sugar chains of glutelin , a major storage protein in rice seeds . The basic subunits of glutelin were shown to be glycoproteins by staining with PAS and the lectins concanavalin A ( Con A ) and peanut agglutinin ( PNA ) . The binding of PNA to the basic subunits was substantially reduced by treatment of the glutelin with NaIO4 , NaOH , or O-glycanase . The sugar chains of the subunits , obtained by hydrazinolysis and O-glycanase , were pyridylaminated and subjected to 2-dimensional HPLC analysis using C ( 18 ) and acrylamide-derivatized columns . It was found that the Galbeta-1 , 3GalNAc disaccharide , which was previously identified as a core 1 structure of mucin-type sugar chains , is conjugated to the glutelin subunits . Furthermore , amino acid sequencing of an 11-kDa peptide of the subunits , recognized by both PNA and Con A , suggested that both N-linked and O-linked glycosylation occurs in the carboxy-terminal region of these subunits .
[ Sen. 3, subscore: 1.00 ]: Characterization was done on the sugar chains of glutelin , a major storage protein in rice seeds . The basic subunits of glutelin were shown to be glycoproteins by staining with PAS and the lectins concanavalin A ( Con A ) and peanut agglutinin ( PNA ) . The binding of PNA to the basic subunits was substantially reduced by treatment of the glutelin with NaIO4 , NaOH , or O-glycanase . The sugar chains of the subunits , obtained by hydrazinolysis and O-glycanase , were pyridylaminated and subjected to 2-dimensional HPLC analysis using C ( 18 ) and acrylamide-derivatized columns . It was found that the Galbeta-1 , 3GalNAc disaccharide , which was previously identified as a core 1 structure of mucin-type sugar chains , is conjugated to the glutelin subunits . Furthermore , amino acid sequencing of an 11-kDa peptide of the subunits , recognized by both PNA and Con A , suggested that both N-linked and O-linked glycosylation occurs in the carboxy-terminal region of these subunits .
[ Sen. 6, subscore: 1.00 ]: The basic subunits of glutelin were shown to be glycoproteins by staining with PAS and the lectins concanavalin A ( Con A ) and peanut agglutinin ( PNA ) . The binding of PNA to the basic subunits was substantially reduced by treatment of the glutelin with NaIO4 , NaOH , or O-glycanase . The sugar chains of the subunits , obtained by hydrazinolysis and O-glycanase , were pyridylaminated and subjected to 2-dimensional HPLC analysis using C ( 18 ) and acrylamide-derivatized columns . It was found that the Galbeta-1 , 3GalNAc disaccharide , which was previously identified as a core 1 structure of mucin-type sugar chains , is conjugated to the glutelin subunits . Furthermore , amino acid sequencing of an 11-kDa peptide of the subunits , recognized by both PNA and Con A , suggested that both N-linked and O-linked glycosylation occurs in the carboxy-terminal region of these subunits .
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Score: 6.00 |
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| Title: Developmentally regulated expression of a peptide : N-glycanase during germination of rice seeds ( Oryza sativa ) and its purification and characterization .
| | Author: Chang T Kuo MC Khoo KH Inoue S Inoue Y | | Journal: J Biol . Chem . Citation: V : 275 ( 1 ) P : 129-34 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10617595 | | Abstract: Peptide : N-glycanase ( PNGase ; EC 3 . 5 . 1 . 52 ) activity was detected in dormant rice seeds ( Oryza sativa ) and the imbibed rice grains .
Time-course studies revealed that the enzyme activity remained almost constant until about 30 h after imbibition in both of endosperm and embryo it issue-containing areas , and started to increase only in growing germ part , reached a peak at about 3-day stage , followed by a gradual decrease concomitant with a sharp increase in the coleoptile .
The specific activity increased about 6-fold at about 3-day stage .
PNGase was purified to electrophoretic homogeneity from the extracts of germinated rice seeds at 24 h , and the apparent molecular weight of the purified enzyme , estimated by SDS-polyacrylamide gel electrophoresis ( SDS-PAGE ) , was about 80 , 000 .
The purified enzyme was designated PNGase Os to denote its origin .
The N-terminal sequence of the 10 residues was determined to be SYNVASVAGL .
The purified PNGase Os in SDS-PAGE appeared as a rather broad band , consistent with the presence of multiple glycoforms as indicated by chromatographic behavior on a Sephadex G-75 column .
PNGase expressed in coleoptile under anoxia condition was also purified , and both of the purified enzymes were found to exhibit very similar , if not identical , electrophoretic mobility in SDS-PAGE .
PNGase Os exhibited a broad pH-activity profile with an optimum of 4-5 and , interestingly , was significantly inactivated by K ( + ) and Na ( + ) at near the physiological concentration , 100 mM .
These results are discussed in relation to other work . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Peptide : N-glycanase ( PNGase ; EC 3 . 5 . 1 . 52 ) activity was detected in dormant rice seeds ( Oryza sativa ) and the imbibed rice grains . Time-course studies revealed that the enzyme activity remained almost constant until about 30 h after imbibition in both of endosperm and embryo it issue-containing areas , and started to increase only in growing germ part , reached a peak at about 3-day stage , followed by a gradual decrease concomitant with a sharp increase in the coleoptile . The specific activity increased about 6-fold at about 3-day stage . PNGase was purified to electrophoretic homogeneity from the extracts of germinated rice seeds at 24 h , and the apparent molecular weight of the purified enzyme , estimated by SDS-polyacrylamide gel electrophoresis ( SDS-PAGE ) , was about 80 , 000 . The purified enzyme was designated PNGase Os to denote its origin .
[ Sen. 4, subscore: 1.00 ]: Peptide : N-glycanase ( PNGase ; EC 3 . 5 . 1 . 52 ) activity was detected in dormant rice seeds ( Oryza sativa ) and the imbibed rice grains . Time-course studies revealed that the enzyme activity remained almost constant until about 30 h after imbibition in both of endosperm and embryo it issue-containing areas , and started to increase only in growing germ part , reached a peak at about 3-day stage , followed by a gradual decrease concomitant with a sharp increase in the coleoptile . The specific activity increased about 6-fold at about 3-day stage . PNGase was purified to electrophoretic homogeneity from the extracts of germinated rice seeds at 24 h , and the apparent molecular weight of the purified enzyme , estimated by SDS-polyacrylamide gel electrophoresis ( SDS-PAGE ) , was about 80 , 000 . The purified enzyme was designated PNGase Os to denote its origin . The N-terminal sequence of the 10 residues was determined to be SYNVASVAGL . The purified PNGase Os in SDS-PAGE appeared as a rather broad band , consistent with the presence of multiple glycoforms as indicated by chromatographic behavior on a Sephadex G-75 column . PNGase expressed in coleoptile under anoxia condition was also purified , and both of the purified enzymes were found to exhibit very similar , if not identical , electrophoretic mobility in SDS-PAGE .
[ Sen. 5, subscore: 1.00 ]: Peptide : N-glycanase ( PNGase ; EC 3 . 5 . 1 . 52 ) activity was detected in dormant rice seeds ( Oryza sativa ) and the imbibed rice grains . Time-course studies revealed that the enzyme activity remained almost constant until about 30 h after imbibition in both of endosperm and embryo it issue-containing areas , and started to increase only in growing germ part , reached a peak at about 3-day stage , followed by a gradual decrease concomitant with a sharp increase in the coleoptile . The specific activity increased about 6-fold at about 3-day stage . PNGase was purified to electrophoretic homogeneity from the extracts of germinated rice seeds at 24 h , and the apparent molecular weight of the purified enzyme , estimated by SDS-polyacrylamide gel electrophoresis ( SDS-PAGE ) , was about 80 , 000 . The purified enzyme was designated PNGase Os to denote its origin . The N-terminal sequence of the 10 residues was determined to be SYNVASVAGL . The purified PNGase Os in SDS-PAGE appeared as a rather broad band , consistent with the presence of multiple glycoforms as indicated by chromatographic behavior on a Sephadex G-75 column . PNGase expressed in coleoptile under anoxia condition was also purified , and both of the purified enzymes were found to exhibit very similar , if not identical , electrophoretic mobility in SDS-PAGE . PNGase Os exhibited a broad pH-activity profile with an optimum of 4-5 and , interestingly , was significantly inactivated by K ( + ) and Na ( + ) at near the physiological concentration , 100 mM .
[ Sen. 7, subscore: 1.00 ]: The specific activity increased about 6-fold at about 3-day stage . PNGase was purified to electrophoretic homogeneity from the extracts of germinated rice seeds at 24 h , and the apparent molecular weight of the purified enzyme , estimated by SDS-polyacrylamide gel electrophoresis ( SDS-PAGE ) , was about 80 , 000 . The purified enzyme was designated PNGase Os to denote its origin . The N-terminal sequence of the 10 residues was determined to be SYNVASVAGL . The purified PNGase Os in SDS-PAGE appeared as a rather broad band , consistent with the presence of multiple glycoforms as indicated by chromatographic behavior on a Sephadex G-75 column . PNGase expressed in coleoptile under anoxia condition was also purified , and both of the purified enzymes were found to exhibit very similar , if not identical , electrophoretic mobility in SDS-PAGE . PNGase Os exhibited a broad pH-activity profile with an optimum of 4-5 and , interestingly , was significantly inactivated by K ( + ) and Na ( + ) at near the physiological concentration , 100 mM . These results are discussed in relation to other work .
[ Sen. 8, subscore: 1.00 ]: PNGase was purified to electrophoretic homogeneity from the extracts of germinated rice seeds at 24 h , and the apparent molecular weight of the purified enzyme , estimated by SDS-polyacrylamide gel electrophoresis ( SDS-PAGE ) , was about 80 , 000 . The purified enzyme was designated PNGase Os to denote its origin . The N-terminal sequence of the 10 residues was determined to be SYNVASVAGL . The purified PNGase Os in SDS-PAGE appeared as a rather broad band , consistent with the presence of multiple glycoforms as indicated by chromatographic behavior on a Sephadex G-75 column . PNGase expressed in coleoptile under anoxia condition was also purified , and both of the purified enzymes were found to exhibit very similar , if not identical , electrophoretic mobility in SDS-PAGE . PNGase Os exhibited a broad pH-activity profile with an optimum of 4-5 and , interestingly , was significantly inactivated by K ( + ) and Na ( + ) at near the physiological concentration , 100 mM . These results are discussed in relation to other work .
[ Sen. 9, subscore: 1.00 ]: The purified enzyme was designated PNGase Os to denote its origin . The N-terminal sequence of the 10 residues was determined to be SYNVASVAGL . The purified PNGase Os in SDS-PAGE appeared as a rather broad band , consistent with the presence of multiple glycoforms as indicated by chromatographic behavior on a Sephadex G-75 column . PNGase expressed in coleoptile under anoxia condition was also purified , and both of the purified enzymes were found to exhibit very similar , if not identical , electrophoretic mobility in SDS-PAGE . PNGase Os exhibited a broad pH-activity profile with an optimum of 4-5 and , interestingly , was significantly inactivated by K ( + ) and Na ( + ) at near the physiological concentration , 100 mM . These results are discussed in relation to other work .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: Genotypic characterization of drug-resistant Mycobacterium tuberculosis isolates from Peru .
| | Author: Escalante P Ramaswamy S Sanabria H Soini H Pan X Valiente-Castillo O Musser JM .
| | Journal: Tuber .
Lung Dis .
Citation: V : 79 ( 2 ) P : 111-8 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10645449 | | Abstract: SETTING : Twenty-nine epidemiological unrelated and mostly multidrug-resistant Mycobacterium tuberculosis ( MDR-TB ) strains from Peruvian patients .
OBJECTIVE : To investigate the molecular genetics of MDR-TB strains recovered in a Latin American country .
DESIGN : Antimicrobial agent susceptibility testing , major genetic group designation , IS6110 fingerprinting , spoligotyping , and automated deoxyribonucleic acid sequencing of regions of the katG , rpoB , embB , gyrA , and pncA genes with mutations commonly associated with drug resistance .
RESULTS : Nineteen isolates were found to be multidrug-resistant by susceptibility testing .
IS6110 typing showed that virtually all isolates were unique and therefore had independently acquired drug resistance .
Seventy-nine percent of isoniazid-resistant strains had a Ser315Thr amino acid change in KatG .
Ninety-five percent of rifampin-resistant isolates had amino acid replacements in the rifampin-resistance determining region of RpoB .
Six of 11 ethambutol-resistant strains had EmbB alterations .
Eleven pyrazinamide-resistant strains had distinct mutations in pncA .
CONCLUSION : Virtually all organisms evolved drug resistance independently .
The types of drug resistance-associated mutations identified were very similar to changes occurring in isolates from other areas of the world .
Nucleotide sequence-based strategies for rapid detection of drug resistance-conferring mutants will be applicable to organisms recovered in Peru , and potentially other areas of Latin America . | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: SETTING : Twenty-nine epidemiological unrelated and mostly multidrug-resistant Mycobacterium tuberculosis ( MDR-TB ) strains from Peruvian patients . OBJECTIVE : To investigate the molecular genetics of MDR-TB strains recovered in a Latin American country . DESIGN : Antimicrobial agent susceptibility testing , major genetic group designation , IS6110 fingerprinting , spoligotyping , and automated deoxyribonucleic acid sequencing of regions of the katG , rpoB , embB , gyrA , and pncA genes with mutations commonly associated with drug resistance . RESULTS : Nineteen isolates were found to be multidrug-resistant by susceptibility testing . IS6110 typing showed that virtually all isolates were unique and therefore had independently acquired drug resistance . Seventy-nine percent of isoniazid-resistant strains had a Ser315Thr amino acid change in KatG . Ninety-five percent of rifampin-resistant isolates had amino acid replacements in the rifampin-resistance determining region of RpoB .
[ Sen. 9, subscore: 1.00 ]: IS6110 typing showed that virtually all isolates were unique and therefore had independently acquired drug resistance . Seventy-nine percent of isoniazid-resistant strains had a Ser315Thr amino acid change in KatG . Ninety-five percent of rifampin-resistant isolates had amino acid replacements in the rifampin-resistance determining region of RpoB . Six of 11 ethambutol-resistant strains had EmbB alterations . Eleven pyrazinamide-resistant strains had distinct mutations in pncA . CONCLUSION : Virtually all organisms evolved drug resistance independently . The types of drug resistance-associated mutations identified were very similar to changes occurring in isolates from other areas of the world . Nucleotide sequence-based strategies for rapid detection of drug resistance-conferring mutants will be applicable to organisms recovered in Peru , and potentially other areas of Latin America .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: Sequence analysis of Pns11 , a nonstructural protein of rice gall dwarf virus , and its expression and detection in infected rice plants and vector insects .
| | Author: Moriyasu Y Ishikawa K Kikuchi A Imanishi S Tomita S Akutsu K Omura T | | Journal: Virus Genes Citation: V : 20 ( 3 ) P : 237-41 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10949951 | | Abstract: The nucleotide sequence of genome segment S11 of rice gall dwarf virus ( RGDV ) , a member of Phytoreovirus , was determined .
The segment encodes a putative protein of 40 kDa that exhibits approximately 37% homology at the amino acid level to the nonstructural proteins Pns10 of rice dwarf and wound tumor viruses , which are other members of Phytoreovirus .
A band of a protein with an apparent molecular mass of 40 kDa was specifically detected in an analysis of cells transfected with S11 cDNA .
An antiserum raised against this protein reacted with a protein of approximately 40kDa after fractionation by SDS-PAGE of materials prepared from infected plants and from viruliferous vector insects .
However , the antiserum did not react with purified viral proteins .
These results suggest that S11 encodes a nonstructural protein of RGDV .
This protein was named Pns11 . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: The nucleotide sequence of genome segment S11 of rice gall dwarf virus ( RGDV ) , a member of Phytoreovirus , was determined . The segment encodes a putative protein of 40 kDa that exhibits approximately 37% homology at the amino acid level to the nonstructural proteins Pns10 of rice dwarf and wound tumor viruses , which are other members of Phytoreovirus . A band of a protein with an apparent molecular mass of 40 kDa was specifically detected in an analysis of cells transfected with S11 cDNA . An antiserum raised against this protein reacted with a protein of approximately 40kDa after fractionation by SDS-PAGE of materials prepared from infected plants and from viruliferous vector insects . However , the antiserum did not react with purified viral proteins . These results suggest that S11 encodes a nonstructural protein of RGDV .
[ Sen. 7, subscore: 1.00 ]: A band of a protein with an apparent molecular mass of 40 kDa was specifically detected in an analysis of cells transfected with S11 cDNA . An antiserum raised against this protein reacted with a protein of approximately 40kDa after fractionation by SDS-PAGE of materials prepared from infected plants and from viruliferous vector insects . However , the antiserum did not react with purified viral proteins . These results suggest that S11 encodes a nonstructural protein of RGDV . This protein was named Pns11 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
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| Title: Sequencing of the rpoB gene in Legionella pneumophila and characterization of mutations associated with rifampin resistance in the Legionellaceae .
| | Author: Nielsen K Hindersson P Hoiby N Bangsborg JM .
| | Journal: Antimicrob .
Agents Chemother .
Citation: V : 44 ( 10 ) P : 2679-83 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10991843 | | Abstract: Rifampin in combination with erythromycin is a recommended treatment for severe cases of legionellosis .
Mutations in the rpoB gene are known to cause rifampin resistance in Escherichia coli and Mycobacterium tuberculosis , and the purpose of the present study was to investigate a possible similar resistance mechanism within the members of the family Legionellaceae .
Since the RNA polymerase genes of this genus have never been characterized , the DNA sequence of the Legionella pneumophila rpoB gene was determined by the Vectorette technique for genome walking .
A 4 , 647-bp DNA sequence that contained the open reading frame ( ORF ) of the rpoB gene ( 4 , 104 bp ) and an ORF of 384 bp representing part of the rpoC gene was obtained .
A 316-bp DNA fragment in the center of the L pneumophila rpoB gene , corresponding to a previously described site for mutations leading to rifampin resistance in M tuberculosis , was sequenced from 18 rifampin-resistant Legionella isolates representing four species ( L bozemanii , L longbeachae , L micdadei , and L pneumophila ) , and the sequences were compared to the sequences of the fragments from the parent ( rifampin-sensitive ) strains .
Six single-base mutations which led to amino acid substitutions at five different positions were identified .
A single strain did not contain any mutations in the 316-bp fragment .
This study represents the characterization of a hitherto undescribed resistance mechanism within the family Legionellaceae . | Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: Rifampin in combination with erythromycin is a recommended treatment for severe cases of legionellosis . Mutations in the rpoB gene are known to cause rifampin resistance in Escherichia coli and Mycobacterium tuberculosis , and the purpose of the present study was to investigate a possible similar resistance mechanism within the members of the family Legionellaceae . Since the RNA polymerase genes of this genus have never been characterized , the DNA sequence of the Legionella pneumophila rpoB gene was determined by the Vectorette technique for genome walking . A 4 , 647-bp DNA sequence that contained the open reading frame ( ORF ) of the rpoB gene ( 4 , 104 bp ) and an ORF of 384 bp representing part of the rpoC gene was obtained . A 316-bp DNA fragment in the center of the L pneumophila rpoB gene , corresponding to a previously described site for mutations leading to rifampin resistance in M tuberculosis , was sequenced from 18 rifampin-resistant Legionella isolates representing four species ( L bozemanii , L longbeachae , L micdadei , and L pneumophila ) , and the sequences were compared to the sequences of the fragments from the parent ( rifampin-sensitive ) strains . Six single-base mutations which led to amino acid substitutions at five different positions were identified . A single strain did not contain any mutations in the 316-bp fragment . This study represents the characterization of a hitherto undescribed resistance mechanism within the family Legionellaceae .
[ Sen. 3, subscore: 1.00 ]: Rifampin in combination with erythromycin is a recommended treatment for severe cases of legionellosis . Mutations in the rpoB gene are known to cause rifampin resistance in Escherichia coli and Mycobacterium tuberculosis , and the purpose of the present study was to investigate a possible similar resistance mechanism within the members of the family Legionellaceae . Since the RNA polymerase genes of this genus have never been characterized , the DNA sequence of the Legionella pneumophila rpoB gene was determined by the Vectorette technique for genome walking . A 4 , 647-bp DNA sequence that contained the open reading frame ( ORF ) of the rpoB gene ( 4 , 104 bp ) and an ORF of 384 bp representing part of the rpoC gene was obtained . A 316-bp DNA fragment in the center of the L pneumophila rpoB gene , corresponding to a previously described site for mutations leading to rifampin resistance in M tuberculosis , was sequenced from 18 rifampin-resistant Legionella isolates representing four species ( L bozemanii , L longbeachae , L micdadei , and L pneumophila ) , and the sequences were compared to the sequences of the fragments from the parent ( rifampin-sensitive ) strains . Six single-base mutations which led to amino acid substitutions at five different positions were identified . A single strain did not contain any mutations in the 316-bp fragment .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Genotypic determination of Mycobacterium tuberculosis antibiotic resistance using a novel mutation detection method , the branch migration inhibition M tuberculosis antibiotic resistance test | | Author: Liu YP Behr MA Small PM Kurn N | | Journal: J Clin . Microbiol . Citation: V : 38 ( 10 ) P : 3656-62 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11015379 | | Abstract: A novel method for the detection of any alteration within a defined sequence has recently been demonstrated ( A Lishanski , N Kurn , and E F Ullman , Nucleic Acids Res .
28 : E42 , 2000 ; A Lishanski , Clin . Chem . 46 : 9 , 2000 ) .
Essential to this method are the generation of partial duplexes that are capable of forming four-stranded structures and the ability to detect inhibition of branch migration in these structures ( I G Panyutin and P Hsieh , J Mol . Biol . 230 : 413-424 , 1993 ) .
Inhibition of branch migration indicates the presence of sequence alteration .
This mutation detection method , termed branch migration inhibition ( BMI ) , is suitable for the detection of drug resistance in M tuberculosis , which is frequently associated with multiple mutations within known genes .
We describe the genotypic determination of the rifampin ( RMP ) and pyrazinamide ( PZA ) susceptibilities of M tuberculosis isolates , using BMI coupled with the luminescence oxygen channeling immunoassay ( LOCI ) ( E F Ullman et al , Proc . Natl . Acad . Sci . USA 91 : 5426-5430 , 1994 ) .
RMP and PZA resistances are associated with multiple mutations within the rpoB and pncA genes , respectively .
M tuberculosis genomic DNA samples prepared from 46 clinical isolates were used for genotypic determination of RMP resistance in a "blind study . " Similarly , PZA resistance was determined using genomic DNA samples prepared from 37 clinical isolates .
Full agreement of the genotypic and phenotypic determinations of drug susceptibility was demonstrated .
RMP susceptibility determination directly from cells of 10 clinical isolates grown in culture was also demonstrated .
The genotypic result of only 1 out of 10 isolates did not agree with the phenotypic susceptibility testing result .
Sequence analysis of the rpoB gene of this clinical isolate revealed a single base substitution , most likely a silent point mutation .
The new BMI-LOCI mutation detection method is a rapid and accurate procedure for the genotypic determination of the RMP and PZA susceptibilities of M tuberculosis clinical isolates .
BMI can also be detected by using commercially available automated enzyme-linked immunosorbent assay plate formats ( Lishanski et al , Nucleic Acids Res .
28 : E42 , 2000 ) . | Matching Sentences:
[ Sen. 7, subscore: 1.00 ]: Essential to this method are the generation of partial duplexes that are capable of forming four-stranded structures and the ability to detect inhibition of branch migration in these structures ( I G Panyutin and P Hsieh , J Mol . Biol . 230 : 413-424 , 1993 ) . Inhibition of branch migration indicates the presence of sequence alteration . This mutation detection method , termed branch migration inhibition ( BMI ) , is suitable for the detection of drug resistance in M tuberculosis , which is frequently associated with multiple mutations within known genes . We describe the genotypic determination of the rifampin ( RMP ) and pyrazinamide ( PZA ) susceptibilities of M tuberculosis isolates , using BMI coupled with the luminescence oxygen channeling immunoassay ( LOCI ) ( E F Ullman et al , Proc . Natl . Acad . Sci . USA 91 : 5426-5430 , 1994 ) . RMP and PZA resistances are associated with multiple mutations within the rpoB and pncA genes , respectively . M tuberculosis genomic DNA samples prepared from 46 clinical isolates were used for genotypic determination of RMP resistance in a "blind study . " Similarly , PZA resistance was determined using genomic DNA samples prepared from 37 clinical isolates . Full agreement of the genotypic and phenotypic determinations of drug susceptibility was demonstrated . RMP susceptibility determination directly from cells of 10 clinical isolates grown in culture was also demonstrated . The genotypic result of only 1 out of 10 isolates did not agree with the phenotypic susceptibility testing result .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: [ A patient with dysphagia treated successfully and discharged without nutritional support ] | | Author: Okuyama Y Nonomura Y Hatanaka N | | Journal: Gan To Kagaku Ryoho Citation: V : 27 Suppl 3 ( ) P : 754-5 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11190340 | | Abstract: One of the main targets of medical care provided in our ward , which specializes in the cooperative practice of hospital and home-doctors , is to maintain the quality of patients lives after they are discharged from our hospital through home medical care by home-doctors .
Intravenous hyperalimentation and tube-feeding at home are suitable solutions for some patients with dysphagia after cerebral infarction .
However , the difficulties faced in their management are the burden on the families , which tends to be an obstacle for at-home-practice .
We describe herein a case of severe dysphagia treated successfully through our rehabilitation program and discharged without nutritional supports .
An 82-year-old man was admitted to our hospital suffering from pyrexia and dysbasia .
The man , who lives with his wife and his sons family , was diagnosed with aspiration pneumonia and multiple cerebral infarctions .
The test for swallowing reflex revealed an impaired first phase reflex and intravenous hyperalimentation was performed for his nutritional support .
He was still suffering from dysphagia but had the desire to eat orally after his dysbasia and aspiration pneumonia were cured .
A rehabilitation program was scheduled with the aims of 1 ) recovery of ingestion and 2 ) sufficient expectoration , with an ongoing teaching program for the management of intravenous hyperalimentation .
After one month of rehabilitation ( ice-massaging , muscle rehabilitation of the tongue and neck and expectoration training in a prone position and after gorging ) , his ability to swallow was gradually recovered .
With the frequent confirmation of absence of aspiration , special forms of diets were served and upgraded from jelly , paste-like-food to soft-cooked steamed rice .
The patient is now at home without any nutritional support .
Nutritional management without intravenous hyperalimentation or tube-feeding is important or even essential for some families providing home-care for patients .
The problem of aging requires us to reduce the burden that families ( who may be also getting older ) should carry .
We try to support patients and families for better home-care through cooperation with society and home-doctors .
| Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: Intravenous hyperalimentation and tube-feeding at home are suitable solutions for some patients with dysphagia after cerebral infarction . However , the difficulties faced in their management are the burden on the families , which tends to be an obstacle for at-home-practice . We describe herein a case of severe dysphagia treated successfully through our rehabilitation program and discharged without nutritional supports . An 82-year-old man was admitted to our hospital suffering from pyrexia and dysbasia . The man , who lives with his wife and his sons family , was diagnosed with aspiration pneumonia and multiple cerebral infarctions . The test for swallowing reflex revealed an impaired first phase reflex and intravenous hyperalimentation was performed for his nutritional support . He was still suffering from dysphagia but had the desire to eat orally after his dysbasia and aspiration pneumonia were cured . A rehabilitation program was scheduled with the aims of 1 ) recovery of ingestion and 2 ) sufficient expectoration , with an ongoing teaching program for the management of intravenous hyperalimentation . After one month of rehabilitation ( ice-massaging , muscle rehabilitation of the tongue and neck and expectoration training in a prone position and after gorging ) , his ability to swallow was gradually recovered .
[ Sen. 8, subscore: 1.00 ]: We describe herein a case of severe dysphagia treated successfully through our rehabilitation program and discharged without nutritional supports . An 82-year-old man was admitted to our hospital suffering from pyrexia and dysbasia . The man , who lives with his wife and his sons family , was diagnosed with aspiration pneumonia and multiple cerebral infarctions . The test for swallowing reflex revealed an impaired first phase reflex and intravenous hyperalimentation was performed for his nutritional support . He was still suffering from dysphagia but had the desire to eat orally after his dysbasia and aspiration pneumonia were cured . A rehabilitation program was scheduled with the aims of 1 ) recovery of ingestion and 2 ) sufficient expectoration , with an ongoing teaching program for the management of intravenous hyperalimentation . After one month of rehabilitation ( ice-massaging , muscle rehabilitation of the tongue and neck and expectoration training in a prone position and after gorging ) , his ability to swallow was gradually recovered . With the frequent confirmation of absence of aspiration , special forms of diets were served and upgraded from jelly , paste-like-food to soft-cooked steamed rice . The patient is now at home without any nutritional support .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: Transposon impala , a novel tool for gene tagging in the rice blast fungus Magnaporthe grisea .
| | Author: Villalba F Lebrun MH Hua-Van A Daboussi MJ Grosjean-Cournoyer MC .
| | Journal: Mol .
Plant Microbe Interact .
Citation: V : 14 ( 3 ) P : 308-15 Year: 2001 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11277428 | | Abstract: impala , a Tc1-mariner transposable element from Fusarium oxysporum , was introduced into the rice blast fungus Magnaporthe grisea to develop transposon-based insertional mutagenesis .
A construct ( pNIL160 ) containing an autonomous impala copy inserted in the promoter of niaD encoding Aspergillus nidulans nitrate reductase was introduced by transformation into a M grisea nitrate reductase-deficient mutant . impala excision was monitored by restoration of prototrophy for nitrate .
Southern analysis of niaD+ revertants revealed that impala was able to excise and reinsert at new loci in M grisea .
As observed for its host Fusarium oxysporum , impala inserted at a TA site left a typical excision footprint of 5 bp .
We have shown that a defective impala copy was inactive in M grisea , yet it can be activated by a functional impala transposase .
A transformant carrying a single copy of pNIL160 was used to generate a collection of 350 revertants .
Mutants either altered for their mycelial growth ( Rev2 ) or nonpathogenic ( Rev77 ) were obtained .
Complementation of Rev77 with a 3-kb genomic fragment from a wild-type locus was successful , demonstrating the tagging of a pathogenicity gene by impala .
This gene , called ORP1 , is essential for penetration of host leaves by M grisea and has no sequence homology to known genes . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: impala , a Tc1-mariner transposable element from Fusarium oxysporum , was introduced into the rice blast fungus Magnaporthe grisea to develop transposon-based insertional mutagenesis . A construct ( pNIL160 ) containing an autonomous impala copy inserted in the promoter of niaD encoding Aspergillus nidulans nitrate reductase was introduced by transformation into a M grisea nitrate reductase-deficient mutant . impala excision was monitored by restoration of prototrophy for nitrate . Southern analysis of niaD+ revertants revealed that impala was able to excise and reinsert at new loci in M grisea . As observed for its host Fusarium oxysporum , impala inserted at a TA site left a typical excision footprint of 5 bp . We have shown that a defective impala copy was inactive in M grisea , yet it can be activated by a functional impala transposase . A transformant carrying a single copy of pNIL160 was used to generate a collection of 350 revertants .
[ Sen. 6, subscore: 1.00 ]: A construct ( pNIL160 ) containing an autonomous impala copy inserted in the promoter of niaD encoding Aspergillus nidulans nitrate reductase was introduced by transformation into a M grisea nitrate reductase-deficient mutant . impala excision was monitored by restoration of prototrophy for nitrate . Southern analysis of niaD+ revertants revealed that impala was able to excise and reinsert at new loci in M grisea . As observed for its host Fusarium oxysporum , impala inserted at a TA site left a typical excision footprint of 5 bp . We have shown that a defective impala copy was inactive in M grisea , yet it can be activated by a functional impala transposase . A transformant carrying a single copy of pNIL160 was used to generate a collection of 350 revertants . Mutants either altered for their mycelial growth ( Rev2 ) or nonpathogenic ( Rev77 ) were obtained . Complementation of Rev77 with a 3-kb genomic fragment from a wild-type locus was successful , demonstrating the tagging of a pathogenicity gene by impala . This gene , called ORP1 , is essential for penetration of host leaves by M grisea and has no sequence homology to known genes .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Rice alpha-mannosidase digesting the high mannose glycopeptide of glutelin .
| | Author: Kishimoto T Hori H Takano D Nakano Y Watanabe M Mitsui T | | Journal: Citation: V : 112 ( 1 ) P : 15-24 Year: 2001 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11319010 | | Abstract: alpha-Mannosidase ( EC 3 . 2 . 1 . 24 ) from rice dry seeds was purified to homogeneity .
Optimum pH and Km for pNP-alpha-Man hydrolysis were pH 4 . 3-4 . 5 and 1 . 04 mM , respectively .
The enzyme digested mannobioses such as Manalpha-1 , 2Man , Manalpha-1 , 6Man , Manalpha-1 , 3Man but Manalpha-1 , 4Man .
Zn2+ ion was required for the activity , whereas EDTA and swainsonine inhibited the activity by 80 and 96% , respectively .
The rice storage protein , glutelin was prepared and its basic subunits were shown to have high mannose-type sugar chains by two-dimensional mapping using NH2-P and C18 silica columns .
They were Man9GlcNAc2 , Man8GlcNAc2 , Man7GlcNAc2 , Man6GlcNAc2 and Man5GlcNAc2 .
All these oligosaccharides were digested by the purified alpha-mannosidase , and Man-GlcNAc2 and mannose were formed .
Glycopeptides , having these high mannose-type sugar chains , could also be digested by the alpha-mannosidase .
Subunits were prepared from glutelin basic subunit and the richest subunit among them , subunit 2 ( isoform 2 ) , was digested by the alpha-mannosidase .
Isoform 2 was digested by V8 protease only partially and slowly .
However , isoform 2 , pre-treated with the alpha-mannosidase , was rapidly and completely digested by V8 protease .
| Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: alpha-Mannosidase ( EC 3 . 2 . 1 . 24 ) from rice dry seeds was purified to homogeneity . Optimum pH and Km for pNP-alpha-Man hydrolysis were pH 4 . 3-4 . 5 and 1 . 04 mM , respectively . The enzyme digested mannobioses such as Manalpha-1 , 2Man , Manalpha-1 , 6Man , Manalpha-1 , 3Man but Manalpha-1 , 4Man . Zn2+ ion was required for the activity , whereas EDTA and swainsonine inhibited the activity by 80 and 96% , respectively . The rice storage protein , glutelin was prepared and its basic subunits were shown to have high mannose-type sugar chains by two-dimensional mapping using NH2-P and C18 silica columns . They were Man9GlcNAc2 , Man8GlcNAc2 , Man7GlcNAc2 , Man6GlcNAc2 and Man5GlcNAc2 .
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Score: 1.00 |
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| Title: Risk factors associated with leptospirosis in northeastern Thailand , 1998 .
| | Author: Tangkanakul W Tharmaphornpil P Plikaytis BD Bragg S Poonsuksombat D Choomkasien P Kingnate D Ashford DA .
| | Journal: Am .
J Trop . Med . Hyg . Citation: V : 63 ( 3-4 ) P : 204-8 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11388516 | | Abstract: Leptospirosis is a zoonotic disease of worldwide distribution caused by spirochetes of the genus Leptospira .
Humans are infected through direct contact with infected animals or through exposure to fresh water or soil contaminated by infected animal urine .
Leptospirosis is characterized by acute fever that can be followed by a more severe , sometimes fatal illness that may include jaundice and renal failure ( Weils disease ) , meningitis , myocarditis , hemorrhagic pneumonitis , or hemodynamic collapse .
To identify potential risk factors for leptospirosis in Thailand , we conducted a matched case-control study in Nakornratchasrima Province of the northeastern region .
Fifty-nine cases and 118 controls were included in the study .
Four activities in the two weeks prior to illness were independently associated with leptospirosis infection : walking through water ( odds ratio [ OR ] = 4 . 9 , 95% confidence interval [ CI ] = 1 . 7-14 . 1 ) , applying fertilizer in wet fields for more than 6 hr a day ( OR = 3 . 4 , 95% CI = 1 . 5-7 . 8 ) , plowing in wet fields for more than 6 hr a day ( OR = 3 . 5 , 95% CI = 1 . 1-11 . 6 ) , and pulling out rice plant sprouts in wet fields for more than 6 hr a day ( OR = 3 . 1 , 95% CI = 1 . 02-9 . 3 ) .
Identification of these risk factors on admission might prove useful for early diagnosis and treatment of leptospirosis in Thailand .
| Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: Leptospirosis is a zoonotic disease of worldwide distribution caused by spirochetes of the genus Leptospira . Humans are infected through direct contact with infected animals or through exposure to fresh water or soil contaminated by infected animal urine . Leptospirosis is characterized by acute fever that can be followed by a more severe , sometimes fatal illness that may include jaundice and renal failure ( Weils disease ) , meningitis , myocarditis , hemorrhagic pneumonitis , or hemodynamic collapse . To identify potential risk factors for leptospirosis in Thailand , we conducted a matched case-control study in Nakornratchasrima Province of the northeastern region . Fifty-nine cases and 118 controls were included in the study . Four activities in the two weeks prior to illness were independently associated with leptospirosis infection : walking through water ( odds ratio [ OR ] = 4 . 9 , 95% confidence interval [ CI ] = 1 . 7-14 . 1 ) , applying fertilizer in wet fields for more than 6 hr a day ( OR = 3 . 4 , 95% CI = 1 . 5-7 . 8 ) , plowing in wet fields for more than 6 hr a day ( OR = 3 . 5 , 95% CI = 1 . 1-11 . 6 ) , and pulling out rice plant sprouts in wet fields for more than 6 hr a day ( OR = 3 . 1 , 95% CI = 1 . 02-9 . 3 ) . Identification of these risk factors on admission might prove useful for early diagnosis and treatment of leptospirosis in Thailand .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 4.00 |
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| Title: [ QTL dissection of panicle number per plant and spikelet number per panicle in rice ( Oryza sativa L ) ] | | Author: Xu JL Xue QZ Luo LJ Li ZK .
| | Journal: Yi Chuan Xue Bao Citation: V : 28 ( 8 ) P : 752-9 Year: 2001 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11554350 | | Abstract: The genetic mechanism underlying panicle number per plant ( PN ) , spikelet number per panicle ( SNP ) and its related traits in rice was analysed using 292 F13 RILs from the cross of Lemont/Teqing and a complete linkage map with 272 molecular markers .
The RILs showed tremendous transgressive segregation for all traits studied .
The weak negative correlation between PN and SNP was observed .
Fifty-one QTLs and 45 epistatic QTL pairs affecting these traits were identified , collectively explaining over 60% of the total variation of individual traits .
Almost all SNP-QTLs were attributable to one or more of its contributing components .
Branching number traits had greater contributions to SNP than length traits , in which the first had twice as many QTLs mapped in the same or near regions with SNP as the latter .
Only two PN-QTLs were mapped in the near regions with those of related traits of SNP , suggesting a reasonable recombination between PN and SNP would be available by marker-assisted selection ( MAS ) .
Some major QTLs including QPn4 for panicle number , QPbn3a , QPbn3b and QPbl4 for panicle branching and length would be of great value in MAS .
It was discussed that a new high-yielding panicle type was resulted from reasonably deploying for QTLs of panicle traits by MAS . | Matching Sentences:
[ Sen. 7, subscore: 2.00 ]: The weak negative correlation between PN and SNP was observed . Fifty-one QTLs and 45 epistatic QTL pairs affecting these traits were identified , collectively explaining over 60% of the total variation of individual traits . Almost all SNP-QTLs were attributable to one or more of its contributing components . Branching number traits had greater contributions to SNP than length traits , in which the first had twice as many QTLs mapped in the same or near regions with SNP as the latter . Only two PN-QTLs were mapped in the near regions with those of related traits of SNP , suggesting a reasonable recombination between PN and SNP would be available by marker-assisted selection ( MAS ) . Some major QTLs including QPn4 for panicle number , QPbn3a , QPbn3b and QPbl4 for panicle branching and length would be of great value in MAS . It was discussed that a new high-yielding panicle type was resulted from reasonably deploying for QTLs of panicle traits by MAS .
[ Sen. 1, subscore: 1.00 ]: The genetic mechanism underlying panicle number per plant ( PN ) , spikelet number per panicle ( SNP ) and its related traits in rice was analysed using 292 F13 RILs from the cross of Lemont/Teqing and a complete linkage map with 272 molecular markers . The RILs showed tremendous transgressive segregation for all traits studied . The weak negative correlation between PN and SNP was observed . Fifty-one QTLs and 45 epistatic QTL pairs affecting these traits were identified , collectively explaining over 60% of the total variation of individual traits . Almost all SNP-QTLs were attributable to one or more of its contributing components .
[ Sen. 3, subscore: 1.00 ]: The genetic mechanism underlying panicle number per plant ( PN ) , spikelet number per panicle ( SNP ) and its related traits in rice was analysed using 292 F13 RILs from the cross of Lemont/Teqing and a complete linkage map with 272 molecular markers . The RILs showed tremendous transgressive segregation for all traits studied . The weak negative correlation between PN and SNP was observed . Fifty-one QTLs and 45 epistatic QTL pairs affecting these traits were identified , collectively explaining over 60% of the total variation of individual traits . Almost all SNP-QTLs were attributable to one or more of its contributing components . Branching number traits had greater contributions to SNP than length traits , in which the first had twice as many QTLs mapped in the same or near regions with SNP as the latter . Only two PN-QTLs were mapped in the near regions with those of related traits of SNP , suggesting a reasonable recombination between PN and SNP would be available by marker-assisted selection ( MAS ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Isolation and characterization of triacontanol-regulated genes in rice ( Oryza sativa L ) : possible role of triacontanol as a plant growth stimulator .
| | Author: Chen X Yuan H Chen R Zhu L Du B Weng Q He G | | Journal: Plant Cell Physiol .
Citation: V : 43 ( 8 ) P : 869-76 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12198189 | | Abstract: Triacontanol ( TRIA ) is a saturated long-chain alcohol that is known to have a growth promoting activity when exogenously supplied to a number of plants .
In this study , dry weight , protein and chlorophyll contents of rice seedlings were increased by foliar application of TRIA .
Leaf net photosynthesis rate ( Pn ) was increased very quickly and persistently at a given photon flux density ( PFD ) .
The TRIA-regulated genes in rice were isolated from cDNA library by differential screening with probes generated from the forward and reverse-suppression subtractive hybridization ( SSH ) populations and confirmed by Northern blot .
Sequence analysis revealed that most of the up-regulated genes encoded the photosynthetic and photorespiratory proteins .
Two down-regulated genes were identified as those encoding an ABA and stress-related protein and a wounding-related protein .
These results suggested that TRIA up-regulated the photosynthesis process and suppressed stresses in rice plants .
Time-course profiles of expression of rbcS isogenes suggested the complex mechanisms involved in the regulation of photosynthesis promoted by TRIA . | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: Triacontanol ( TRIA ) is a saturated long-chain alcohol that is known to have a growth promoting activity when exogenously supplied to a number of plants . In this study , dry weight , protein and chlorophyll contents of rice seedlings were increased by foliar application of TRIA . Leaf net photosynthesis rate ( Pn ) was increased very quickly and persistently at a given photon flux density ( PFD ) . The TRIA-regulated genes in rice were isolated from cDNA library by differential screening with probes generated from the forward and reverse-suppression subtractive hybridization ( SSH ) populations and confirmed by Northern blot . Sequence analysis revealed that most of the up-regulated genes encoded the photosynthetic and photorespiratory proteins . Two down-regulated genes were identified as those encoding an ABA and stress-related protein and a wounding-related protein . These results suggested that TRIA up-regulated the photosynthesis process and suppressed stresses in rice plants .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 11.00 |
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| Title: Purification and characterization of beta-N-acetylhexosaminidase from rice seeds .
| | Author: Jin YL Jo YY Kim KY Shim JH Kim YW Park RD .
| | Journal: J Biochem . Mol . Biol . Citation: V : 35 ( 3 ) P : 313-9 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12297015 | | Abstract: N-Acetyl-beta-D-hexosaminidase ( beta-HexNAcase ) ( EC 3 . 2 . 1 . 52 ) was purified from rice seeds ( Oryza sativa L var .
Dongjin ) using ammonium sulfate ( 80% ) precipitation , Sephadex G-150 , CM-Sephadex , and DEAE-Sephadex chromatography , sequentially .
The activities were separated into 7 fractions ( Fsub1 ; - F7sub7 ) by CM-Sephadex chromatography .
Among them , F6 was further purified to homogeneity with a 13 . 0% yield and 123 . 3 purification-fold .
The molecular mass was estimated to be about 52 kDa on SDS-PAGE and 37 . 4 kDa on Sephacryl S 300 gel filtration .
The enzyme catalyzed the hydrolysis of both p-nitrophenyl-N-acetyl-beta-D-glucosaminide ( pNP-GlcNAc ) and p-nitrophenyl-N-acetyl-beta-D-galactosaminide ( pNPGalNAc ) as substrates , which are typical properties of beta-HexNAcase .
The ratio of the pNP-GlcNAcase activity to the pNP-GalNAcase activity was 4 . 0 .
However , it could not hydrolyze chitin , chitosan , pNP-beta-glucopyranoside , or pNP-beta-galactopyranoside .
The enzyme showed K ( M ) , V ( max ) and K ( cat ) for pNP-GlcNAc of 1 . 65mM , 79 . 49mM min ( 1 ) , and 4 . 79 x 10 ( 6 ) min ( 1 ) , respectively .
The comparison of kinetic values for pNPGlcNAc and pNP-GalNAc revealed that the two enzyme activities are associated with a single binding site .
The purified enzyme exhibited optimum pH and temperature for pNPGlcNAc of 5 . 0 and 50 degrees C , respectively .
The enzyme activity for pNP-GlcNAc was stable at pH 5 . 0-5 . 5 and 20-40 degrees C The enzyme activity was completely inhibited at a concentration of 0 . 1 mM HgCl ( 2 ) and AgNO ( 3 ) , suggesting that the intact thiol group is essential for activity .
Chloramine T completely inhibited the activity , indicating the possible involvement of methionines in the mechanism of the enzyme . | Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: Dongjin ) using ammonium sulfate ( 80% ) precipitation , Sephadex G-150 , CM-Sephadex , and DEAE-Sephadex chromatography , sequentially . The activities were separated into 7 fractions ( Fsub1 ; - F7sub7 ) by CM-Sephadex chromatography . Among them , F6 was further purified to homogeneity with a 13 . 0% yield and 123 . 3 purification-fold . The molecular mass was estimated to be about 52 kDa on SDS-PAGE and 37 . 4 kDa on Sephacryl S 300 gel filtration . The enzyme catalyzed the hydrolysis of both p-nitrophenyl-N-acetyl-beta-D-glucosaminide ( pNP-GlcNAc ) and p-nitrophenyl-N-acetyl-beta-D-galactosaminide ( pNPGalNAc ) as substrates , which are typical properties of beta-HexNAcase . The ratio of the pNP-GlcNAcase activity to the pNP-GalNAcase activity was 4 . 0 . However , it could not hydrolyze chitin , chitosan , pNP-beta-glucopyranoside , or pNP-beta-galactopyranoside . The enzyme showed K ( M ) , V ( max ) and K ( cat ) for pNP-GlcNAc of 1 . 65mM , 79 . 49mM min ( 1 ) , and 4 . 79 x 10 ( 6 ) min ( 1 ) , respectively . The comparison of kinetic values for pNPGlcNAc and pNP-GalNAc revealed that the two enzyme activities are associated with a single binding site .
[ Sen. 7, subscore: 2.00 ]: The activities were separated into 7 fractions ( Fsub1 ; - F7sub7 ) by CM-Sephadex chromatography . Among them , F6 was further purified to homogeneity with a 13 . 0% yield and 123 . 3 purification-fold . The molecular mass was estimated to be about 52 kDa on SDS-PAGE and 37 . 4 kDa on Sephacryl S 300 gel filtration . The enzyme catalyzed the hydrolysis of both p-nitrophenyl-N-acetyl-beta-D-glucosaminide ( pNP-GlcNAc ) and p-nitrophenyl-N-acetyl-beta-D-galactosaminide ( pNPGalNAc ) as substrates , which are typical properties of beta-HexNAcase . The ratio of the pNP-GlcNAcase activity to the pNP-GalNAcase activity was 4 . 0 . However , it could not hydrolyze chitin , chitosan , pNP-beta-glucopyranoside , or pNP-beta-galactopyranoside . The enzyme showed K ( M ) , V ( max ) and K ( cat ) for pNP-GlcNAc of 1 . 65mM , 79 . 49mM min ( 1 ) , and 4 . 79 x 10 ( 6 ) min ( 1 ) , respectively . The comparison of kinetic values for pNPGlcNAc and pNP-GalNAc revealed that the two enzyme activities are associated with a single binding site . The purified enzyme exhibited optimum pH and temperature for pNPGlcNAc of 5 . 0 and 50 degrees C , respectively .
[ Sen. 8, subscore: 2.00 ]: Among them , F6 was further purified to homogeneity with a 13 . 0% yield and 123 . 3 purification-fold . The molecular mass was estimated to be about 52 kDa on SDS-PAGE and 37 . 4 kDa on Sephacryl S 300 gel filtration . The enzyme catalyzed the hydrolysis of both p-nitrophenyl-N-acetyl-beta-D-glucosaminide ( pNP-GlcNAc ) and p-nitrophenyl-N-acetyl-beta-D-galactosaminide ( pNPGalNAc ) as substrates , which are typical properties of beta-HexNAcase . The ratio of the pNP-GlcNAcase activity to the pNP-GalNAcase activity was 4 . 0 . However , it could not hydrolyze chitin , chitosan , pNP-beta-glucopyranoside , or pNP-beta-galactopyranoside . The enzyme showed K ( M ) , V ( max ) and K ( cat ) for pNP-GlcNAc of 1 . 65mM , 79 . 49mM min ( 1 ) , and 4 . 79 x 10 ( 6 ) min ( 1 ) , respectively . The comparison of kinetic values for pNPGlcNAc and pNP-GalNAc revealed that the two enzyme activities are associated with a single binding site . The purified enzyme exhibited optimum pH and temperature for pNPGlcNAc of 5 . 0 and 50 degrees C , respectively . The enzyme activity for pNP-GlcNAc was stable at pH 5 . 0-5 . 5 and 20-40 degrees C The enzyme activity was completely inhibited at a concentration of 0 . 1 mM HgCl ( 2 ) and AgNO ( 3 ) , suggesting that the intact thiol group is essential for activity .
[ Sen. 10, subscore: 2.00 ]: The enzyme catalyzed the hydrolysis of both p-nitrophenyl-N-acetyl-beta-D-glucosaminide ( pNP-GlcNAc ) and p-nitrophenyl-N-acetyl-beta-D-galactosaminide ( pNPGalNAc ) as substrates , which are typical properties of beta-HexNAcase . The ratio of the pNP-GlcNAcase activity to the pNP-GalNAcase activity was 4 . 0 . However , it could not hydrolyze chitin , chitosan , pNP-beta-glucopyranoside , or pNP-beta-galactopyranoside . The enzyme showed K ( M ) , V ( max ) and K ( cat ) for pNP-GlcNAc of 1 . 65mM , 79 . 49mM min ( 1 ) , and 4 . 79 x 10 ( 6 ) min ( 1 ) , respectively . The comparison of kinetic values for pNPGlcNAc and pNP-GalNAc revealed that the two enzyme activities are associated with a single binding site . The purified enzyme exhibited optimum pH and temperature for pNPGlcNAc of 5 . 0 and 50 degrees C , respectively . The enzyme activity for pNP-GlcNAc was stable at pH 5 . 0-5 . 5 and 20-40 degrees C The enzyme activity was completely inhibited at a concentration of 0 . 1 mM HgCl ( 2 ) and AgNO ( 3 ) , suggesting that the intact thiol group is essential for activity . Chloramine T completely inhibited the activity , indicating the possible involvement of methionines in the mechanism of the enzyme .
[ Sen. 9, subscore: 1.00 ]: The molecular mass was estimated to be about 52 kDa on SDS-PAGE and 37 . 4 kDa on Sephacryl S 300 gel filtration . The enzyme catalyzed the hydrolysis of both p-nitrophenyl-N-acetyl-beta-D-glucosaminide ( pNP-GlcNAc ) and p-nitrophenyl-N-acetyl-beta-D-galactosaminide ( pNPGalNAc ) as substrates , which are typical properties of beta-HexNAcase . The ratio of the pNP-GlcNAcase activity to the pNP-GalNAcase activity was 4 . 0 . However , it could not hydrolyze chitin , chitosan , pNP-beta-glucopyranoside , or pNP-beta-galactopyranoside . The enzyme showed K ( M ) , V ( max ) and K ( cat ) for pNP-GlcNAc of 1 . 65mM , 79 . 49mM min ( 1 ) , and 4 . 79 x 10 ( 6 ) min ( 1 ) , respectively . The comparison of kinetic values for pNPGlcNAc and pNP-GalNAc revealed that the two enzyme activities are associated with a single binding site . The purified enzyme exhibited optimum pH and temperature for pNPGlcNAc of 5 . 0 and 50 degrees C , respectively . The enzyme activity for pNP-GlcNAc was stable at pH 5 . 0-5 . 5 and 20-40 degrees C The enzyme activity was completely inhibited at a concentration of 0 . 1 mM HgCl ( 2 ) and AgNO ( 3 ) , suggesting that the intact thiol group is essential for activity . Chloramine T completely inhibited the activity , indicating the possible involvement of methionines in the mechanism of the enzyme .
[ Sen. 11, subscore: 1.00 ]: The ratio of the pNP-GlcNAcase activity to the pNP-GalNAcase activity was 4 . 0 . However , it could not hydrolyze chitin , chitosan , pNP-beta-glucopyranoside , or pNP-beta-galactopyranoside . The enzyme showed K ( M ) , V ( max ) and K ( cat ) for pNP-GlcNAc of 1 . 65mM , 79 . 49mM min ( 1 ) , and 4 . 79 x 10 ( 6 ) min ( 1 ) , respectively . The comparison of kinetic values for pNPGlcNAc and pNP-GalNAc revealed that the two enzyme activities are associated with a single binding site . The purified enzyme exhibited optimum pH and temperature for pNPGlcNAc of 5 . 0 and 50 degrees C , respectively . The enzyme activity for pNP-GlcNAc was stable at pH 5 . 0-5 . 5 and 20-40 degrees C The enzyme activity was completely inhibited at a concentration of 0 . 1 mM HgCl ( 2 ) and AgNO ( 3 ) , suggesting that the intact thiol group is essential for activity . Chloramine T completely inhibited the activity , indicating the possible involvement of methionines in the mechanism of the enzyme .
[ Sen. 12, subscore: 1.00 ]: However , it could not hydrolyze chitin , chitosan , pNP-beta-glucopyranoside , or pNP-beta-galactopyranoside . The enzyme showed K ( M ) , V ( max ) and K ( cat ) for pNP-GlcNAc of 1 . 65mM , 79 . 49mM min ( 1 ) , and 4 . 79 x 10 ( 6 ) min ( 1 ) , respectively . The comparison of kinetic values for pNPGlcNAc and pNP-GalNAc revealed that the two enzyme activities are associated with a single binding site . The purified enzyme exhibited optimum pH and temperature for pNPGlcNAc of 5 . 0 and 50 degrees C , respectively . The enzyme activity for pNP-GlcNAc was stable at pH 5 . 0-5 . 5 and 20-40 degrees C The enzyme activity was completely inhibited at a concentration of 0 . 1 mM HgCl ( 2 ) and AgNO ( 3 ) , suggesting that the intact thiol group is essential for activity . Chloramine T completely inhibited the activity , indicating the possible involvement of methionines in the mechanism of the enzyme .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Maporal viral infection in the Syrian golden hamster : a model of hantavirus pulmonary syndrome .
| | Author: Milazzo ML Eyzaguirre EJ Molina CP Fulhorst CF .
| | Journal: J Infect . Dis . Citation: V : 186 ( 10 ) P : 1390-5 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12404153 | | Abstract: Hantavirus pulmonary syndrome ( HPS ) is a severe and often fatal rodent-borne zoonosis .
Maporal ( MAP ) virus is a newly discovered hantavirus that originally was isolated from an arboreal rice rat captured in central Venezuela .
The results of this study indicate that MAP virus in the Syrian golden hamster ( Mesocricetus auratus ) can cause a disease that is clinically and pathologically remarkably similar to HPS .
The similarities include the time course of clinical disease , presence of virus-specific IgG at the onset of clinical disease , subacute pneumonitis , rapid onset of diffuse alveolar edema in the absence of necrosis , hepatic-portal triaditis , mononuclear-cellular infiltrate in lung and liver , widespread distribution of hantaviral antigen in endothelial cells of the microvasculature of lung and other it issues , and variable lethality .
These similarities suggest that the MAP virus-hamster system is a useful model for studies of the pathogenesis of HPS and for the evaluation of potential therapeutic agents .
| Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: Hantavirus pulmonary syndrome ( HPS ) is a severe and often fatal rodent-borne zoonosis . Maporal ( MAP ) virus is a newly discovered hantavirus that originally was isolated from an arboreal rice rat captured in central Venezuela . The results of this study indicate that MAP virus in the Syrian golden hamster ( Mesocricetus auratus ) can cause a disease that is clinically and pathologically remarkably similar to HPS . The similarities include the time course of clinical disease , presence of virus-specific IgG at the onset of clinical disease , subacute pneumonitis , rapid onset of diffuse alveolar edema in the absence of necrosis , hepatic-portal triaditis , mononuclear-cellular infiltrate in lung and liver , widespread distribution of hantaviral antigen in endothelial cells of the microvasculature of lung and other it issues , and variable lethality . These similarities suggest that the MAP virus-hamster system is a useful model for studies of the pathogenesis of HPS and for the evaluation of potential therapeutic agents .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
|---|
| Title: [ Leaf photosynthetic acclimation of Echinochloa crusgalli grown in rice field to free-air CO2 enrichment ( FACE ) ] | | Author: Chen G Liao Y Cai S Zeng Q Zhu J Han Y Liu G Xu D | | Journal: Citation: V : 13 ( 10 ) P : 1201-4 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12557659 | | Abstract: A comparative study was made between the leaves of Echinochloa crusgalli grown at 580 ( FACE ) and 380 ( control , ambient air ) mumol CO2 mol-1 air by observation of net photosynthetic rates ( Pn ) and the contents of soluble protein and the key enzyme of photosynthetic carbon assimilation , Rubisco When measured at the ambient air CO2 concentration ( 380 mumol . mol-1 ) , Pn were significantly lower in leaves grown under FACE conditions than that in those leaves grown in the ambient air .
This indicates that photosynthetic acclimation to high CO2 occurs in the leaves grown under FACE conditions .
Also , the stomatal conductance ( Gs ) and intercellular CO2 concentration ( Ci ) were decreased significantly in the leaves grown under FACE .
The content of total soluble protein was much lower in the leaves grown under FACE conditions than that the in the control , and the content of Rubisco was also decreased in the FACE leaves , but the difference was not significant .
From these results it is deduced that the photosynthetic acclimation to high CO2 in Echinochloa crusgalli leaves grown under FACE conditions is mainly related to the partial closure of stomata and the decrease in soluble protein containing some enzymes . | Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: A comparative study was made between the leaves of Echinochloa crusgalli grown at 580 ( FACE ) and 380 ( control , ambient air ) mumol CO2 mol-1 air by observation of net photosynthetic rates ( Pn ) and the contents of soluble protein and the key enzyme of photosynthetic carbon assimilation , Rubisco When measured at the ambient air CO2 concentration ( 380 mumol . mol-1 ) , Pn were significantly lower in leaves grown under FACE conditions than that in those leaves grown in the ambient air . This indicates that photosynthetic acclimation to high CO2 occurs in the leaves grown under FACE conditions . Also , the stomatal conductance ( Gs ) and intercellular CO2 concentration ( Ci ) were decreased significantly in the leaves grown under FACE . The content of total soluble protein was much lower in the leaves grown under FACE conditions than that the in the control , and the content of Rubisco was also decreased in the FACE leaves , but the difference was not significant . From these results it is deduced that the photosynthetic acclimation to high CO2 in Echinochloa crusgalli leaves grown under FACE conditions is mainly related to the partial closure of stomata and the decrease in soluble protein containing some enzymes .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
|---|
| Title: [ Response and acclimation of photosynthesis in rice leaves to free-air CO2 enrichment ( FACE ) ] | | Author: Liao Y Chen G Zhang H Cai S Zhu J Han Y Liu G Xu D | | Journal: Citation: V : 13 ( 10 ) P : 1205-9 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12557660 | | Abstract: The net photosynthetic rate ( Pn ) , water use efficiency ( WUE ) , and apparent quantum yield of carbon assimilation of rice leaves were boserved contrastively under ambient air ( 380 mumol . mol-1 CO2 ) and FACE ( 580 mumol . mol-1CO2 ) .
The results showed that the observed index under FACE were significantly higher than those under ambient air .
Nevertheless , along with the time of high CO2 treatment prolonged , the enhancement effect of high CO2 on net photosynthetic rate declined gradually .
At the same CO2 concentration , Pn and carboxylation efficiency ( CE ) in rice leaves under FACE were lower than those under ambient air .
Although the stomatal conductances in FACE leaves was obviously lower than that in ambient leaves , their intercellular CO2 concentrations were not significantly different , which implied that the photosynthetic down-regulation in rice leaves grown under FACE was not caused by the decrease of stomatal conductance . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The net photosynthetic rate ( Pn ) , water use efficiency ( WUE ) , and apparent quantum yield of carbon assimilation of rice leaves were boserved contrastively under ambient air ( 380 mumol . mol-1 CO2 ) and FACE ( 580 mumol . mol-1CO2 ) . The results showed that the observed index under FACE were significantly higher than those under ambient air . Nevertheless , along with the time of high CO2 treatment prolonged , the enhancement effect of high CO2 on net photosynthetic rate declined gradually . At the same CO2 concentration , Pn and carboxylation efficiency ( CE ) in rice leaves under FACE were lower than those under ambient air . Although the stomatal conductances in FACE leaves was obviously lower than that in ambient leaves , their intercellular CO2 concentrations were not significantly different , which implied that the photosynthetic down-regulation in rice leaves grown under FACE was not caused by the decrease of stomatal conductance .
[ Sen. 4, subscore: 1.00 ]: The net photosynthetic rate ( Pn ) , water use efficiency ( WUE ) , and apparent quantum yield of carbon assimilation of rice leaves were boserved contrastively under ambient air ( 380 mumol . mol-1 CO2 ) and FACE ( 580 mumol . mol-1CO2 ) . The results showed that the observed index under FACE were significantly higher than those under ambient air . Nevertheless , along with the time of high CO2 treatment prolonged , the enhancement effect of high CO2 on net photosynthetic rate declined gradually . At the same CO2 concentration , Pn and carboxylation efficiency ( CE ) in rice leaves under FACE were lower than those under ambient air . Although the stomatal conductances in FACE leaves was obviously lower than that in ambient leaves , their intercellular CO2 concentrations were not significantly different , which implied that the photosynthetic down-regulation in rice leaves grown under FACE was not caused by the decrease of stomatal conductance .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Long-term feeding effects of heated and fried oils on hepatic antioxidant enzymes , absorption and excretion of fat in rats .
| | Author: Purushothama S Ramachandran HD Narasimhamurthy K Raina PL .
| | Journal: Mol . Cell . Biochem . Citation: V : 247 ( 1-2 ) P : 95-9 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12841636 | | Abstract: Long-term feeding effect of heated and fried peanut ( PNO ) , rice bran ( RBO ) and palm oil ( PO ) in the diet on the hepatic antioxidant enzyme status and absorption and excretion of fats were studied in laboratory rats .
The rats were fed oils heated to 180 degrees C continuously for a period of 72 h or laboratory fried at 20% level in the diet for 18 weeks .
The results of the study indicated a significant increase in the catalase activity in HO groups and decrease in the FRO groups .
The GPx activity while significantly low in HO groups was high in FRO groups , whereas , significant decrease in GST activity was observed in both PNO-HO/FRO groups .
Increased activity was noted in RBO-FRO and PO-HO/FRO groups .
The SOD activity showed a mixed response in different heated/fried oils and a marginal increase in the levels of fecal fat excretion was observed in some of the heated/fried oil groups .
The results indicated no appreciable damage with respect to these antioxidant enzymes .
Also , feeding heated fats as high as 20% in the diet for long duration does not result either in reduced food intake or excess fecal fat excretion .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Long-term feeding effect of heated and fried peanut ( PNO ) , rice bran ( RBO ) and palm oil ( PO ) in the diet on the hepatic antioxidant enzyme status and absorption and excretion of fats were studied in laboratory rats . The rats were fed oils heated to 180 degrees C continuously for a period of 72 h or laboratory fried at 20% level in the diet for 18 weeks . The results of the study indicated a significant increase in the catalase activity in HO groups and decrease in the FRO groups . The GPx activity while significantly low in HO groups was high in FRO groups , whereas , significant decrease in GST activity was observed in both PNO-HO/FRO groups . Increased activity was noted in RBO-FRO and PO-HO/FRO groups .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 7.00 |
|---|
| Title: Molecular marker dissection of rice ( Oryza sativa L ) plant architecture under temperate and tropical climates .
| | Author: Kobayashi S Fukuta Y Sato T Osaki M Khush GS .
| | Journal: Theor . Appl . Genet . Citation: V : 107 ( 8 ) P : 1350-6 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12920520 | | Abstract: Rice ( Oryza sativa L ) plants develop vertically with shoot elongation and horizontally with tillering .
The purpose of this study was to identify and characterize genomic regions influencing the rice plant architecture by quantitative trait locus ( QTL ) analysis for the component traits : culm length ( CL ) , panicle length ( PnL ) , panicle number ( PnN ) and tiller number ( TN ) .
For this QTL analysis , 191 recombinant inbred lines ( F ( 7 ) ) derived from a cross of Milyang 23 ( M23 ) and Akihikari ( AK ) were grown in 1995 , 1996 and 1997 ( May-Oct ) in Joetsu , Japan ( temperate climate ) , and in the 2000 dry season ( Jan-Apr ) , the 2000 wet season ( Jun-Oct ) and the 2001 dry season in Los Baos , The Philippines ( tropical climate ) .
Results showed that rice plant architecture was influenced by 19 genomic regions categorized into five groups .
In Group I , two regions ( on chrs .
6 and 11 ) affected shoot elongation ( CL and PnL ) and tillering ( PnN and TN ) in opposite directions more significantly in Los Baos than in Joetsu .
In Group II , two regions ( chrs .
3 and 12 ) affected shoot elongation , whereas in Group III , five regions [ chrs .
1 ( two ) , 2 , 3 and 9 ] affected only culm length ( CL ) .
Expressions of four regions of Group III were influenced by either tropical or temperate environments .
In Group IV , seven regions ( chrs .
1 , 2 , 4 , 5 , 6 , 8 and 9 ) controlled panicle development ( PnN or PnL ) , and in Group V , three regions ( chrs .
1 , 2 and 3 ) regulated tillering ( PnN or TN ) .
Characterizing these 19 genomic regions provided a detailed analysis of rice plant architecture with emphasis on the multiple effect and environmental responsive regions . | Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: Rice ( Oryza sativa L ) plants develop vertically with shoot elongation and horizontally with tillering . The purpose of this study was to identify and characterize genomic regions influencing the rice plant architecture by quantitative trait locus ( QTL ) analysis for the component traits : culm length ( CL ) , panicle length ( PnL ) , panicle number ( PnN ) and tiller number ( TN ) . For this QTL analysis , 191 recombinant inbred lines ( F ( 7 ) ) derived from a cross of Milyang 23 ( M23 ) and Akihikari ( AK ) were grown in 1995 , 1996 and 1997 ( May-Oct ) in Joetsu , Japan ( temperate climate ) , and in the 2000 dry season ( Jan-Apr ) , the 2000 wet season ( Jun-Oct ) and the 2001 dry season in Los Baos , The Philippines ( tropical climate ) . Results showed that rice plant architecture was influenced by 19 genomic regions categorized into five groups . In Group I , two regions ( on chrs . 6 and 11 ) affected shoot elongation ( CL and PnL ) and tillering ( PnN and TN ) in opposite directions more significantly in Los Baos than in Joetsu .
[ Sen. 6, subscore: 2.00 ]: The purpose of this study was to identify and characterize genomic regions influencing the rice plant architecture by quantitative trait locus ( QTL ) analysis for the component traits : culm length ( CL ) , panicle length ( PnL ) , panicle number ( PnN ) and tiller number ( TN ) . For this QTL analysis , 191 recombinant inbred lines ( F ( 7 ) ) derived from a cross of Milyang 23 ( M23 ) and Akihikari ( AK ) were grown in 1995 , 1996 and 1997 ( May-Oct ) in Joetsu , Japan ( temperate climate ) , and in the 2000 dry season ( Jan-Apr ) , the 2000 wet season ( Jun-Oct ) and the 2001 dry season in Los Baos , The Philippines ( tropical climate ) . Results showed that rice plant architecture was influenced by 19 genomic regions categorized into five groups . In Group I , two regions ( on chrs . 6 and 11 ) affected shoot elongation ( CL and PnL ) and tillering ( PnN and TN ) in opposite directions more significantly in Los Baos than in Joetsu . In Group II , two regions ( chrs . 3 and 12 ) affected shoot elongation , whereas in Group III , five regions [ chrs . 1 ( two ) , 2 , 3 and 9 ] affected only culm length ( CL ) . Expressions of four regions of Group III were influenced by either tropical or temperate environments .
[ Sen. 12, subscore: 2.00 ]: 3 and 12 ) affected shoot elongation , whereas in Group III , five regions [ chrs . 1 ( two ) , 2 , 3 and 9 ] affected only culm length ( CL ) . Expressions of four regions of Group III were influenced by either tropical or temperate environments . In Group IV , seven regions ( chrs . 1 , 2 , 4 , 5 , 6 , 8 and 9 ) controlled panicle development ( PnN or PnL ) , and in Group V , three regions ( chrs . 1 , 2 and 3 ) regulated tillering ( PnN or TN ) . Characterizing these 19 genomic regions provided a detailed analysis of rice plant architecture with emphasis on the multiple effect and environmental responsive regions .
[ Sen. 13, subscore: 1.00 ]: 1 ( two ) , 2 , 3 and 9 ] affected only culm length ( CL ) . Expressions of four regions of Group III were influenced by either tropical or temperate environments . In Group IV , seven regions ( chrs . 1 , 2 , 4 , 5 , 6 , 8 and 9 ) controlled panicle development ( PnN or PnL ) , and in Group V , three regions ( chrs . 1 , 2 and 3 ) regulated tillering ( PnN or TN ) . Characterizing these 19 genomic regions provided a detailed analysis of rice plant architecture with emphasis on the multiple effect and environmental responsive regions .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 8.00 |
|---|
| Title: [ Analysis of additive and AE interaction effects of QTLs controlling plant height , heading date and panicle number in rice ( Oryza sativa L ) ] | | Author: Yuan AP Cao LY Zhuang JY Li RZ Zheng KL Zhu J Cheng SH .
| | Journal: Yi Chuan Xue Bao Citation: V : 30 ( 10 ) P : 899-906 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub14669505 | | Abstract: Plant height ( PH ) , heading date ( HD ) and productive panicle number ( PN ) are important agronomic trait in rice .
Appropriate plant height , heading date and panicle number are prerequisites for the desired high and stable yield level in rice breeding programs .
A recombinant inbred line ( RIL ) population consisting of 304 individuals was derived from a cross between indica varieties Zhong156 and Gumei2 , from which a linkage map consisting of 168 RFLP , SSLP , RAPD and RGA markers that distribute on all the 12 rice chromosomes was constructed , and covers 1447 . 9 cM of the rice genome .
The parents and 304 F9 lines were grown in the paddy field in China National Rice Research Institute ( CNRRI ) , Hangzhou , China in 2001 .
The experiments were carried out in two seasons followed a randomized complete block design .
QTLMapper 1 . 01 was applied to detect QTLs and QTL x environment ( QE ) interaction for HD ( heading data ) , PH ( plant height ) and PN ( panicle number ) , and conditional mapping for PH and PN was performed as well .
A total of 15 QTLs with significant additive effects were detected , among which 4 QTLs had significant QE interaction .
Ten QTLs with additive x additive epistatic effects for PH , HD and PN were detected , among which none showed significant epistatisis x environment interaction .
These QTLs explained 12 . 12% , 1 . 38% and 5 . 00% of the total phenotypic variance for PH , HD and PN , respectively , and contributions were generally lower due to the strong epistatic effects .
In conditional QTL analysis , the numbers of QTLs showing significant additive and epistatic effects were 7 and 6 for PH , and 3 and 3 for PN , respectively .
Among the QTLs having significant additive effects for PH , qPH7-2 showed both additive effects and QE interaction , qPH7-1 and qPH10 showed QE interaction only , and the remaining 4 QTLs showed additive effects only .
Each of the 3 QTLs having significant additive effects for PN did not display significant QE interaction .
No epistatic QE interactions was detected .
In addition , conditional QTL analysis indicated that the expression of QTLs for PH and PN may vary depending on the QTLs for HD . | Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: Appropriate plant height , heading date and panicle number are prerequisites for the desired high and stable yield level in rice breeding programs . A recombinant inbred line ( RIL ) population consisting of 304 individuals was derived from a cross between indica varieties Zhong156 and Gumei2 , from which a linkage map consisting of 168 RFLP , SSLP , RAPD and RGA markers that distribute on all the 12 rice chromosomes was constructed , and covers 1447 . 9 cM of the rice genome . The parents and 304 F9 lines were grown in the paddy field in China National Rice Research Institute ( CNRRI ) , Hangzhou , China in 2001 . The experiments were carried out in two seasons followed a randomized complete block design . QTLMapper 1 . 01 was applied to detect QTLs and QTL x environment ( QE ) interaction for HD ( heading data ) , PH ( plant height ) and PN ( panicle number ) , and conditional mapping for PH and PN was performed as well . A total of 15 QTLs with significant additive effects were detected , among which 4 QTLs had significant QE interaction . Ten QTLs with additive x additive epistatic effects for PH , HD and PN were detected , among which none showed significant epistatisis x environment interaction . These QTLs explained 12 . 12% , 1 . 38% and 5 . 00% of the total phenotypic variance for PH , HD and PN , respectively , and contributions were generally lower due to the strong epistatic effects . In conditional QTL analysis , the numbers of QTLs showing significant additive and epistatic effects were 7 and 6 for PH , and 3 and 3 for PN , respectively .
[ Sen. 1, subscore: 1.00 ]: Plant height ( PH ) , heading date ( HD ) and productive panicle number ( PN ) are important agronomic trait in rice . Appropriate plant height , heading date and panicle number are prerequisites for the desired high and stable yield level in rice breeding programs . A recombinant inbred line ( RIL ) population consisting of 304 individuals was derived from a cross between indica varieties Zhong156 and Gumei2 , from which a linkage map consisting of 168 RFLP , SSLP , RAPD and RGA markers that distribute on all the 12 rice chromosomes was constructed , and covers 1447 . 9 cM of the rice genome . The parents and 304 F9 lines were grown in the paddy field in China National Rice Research Institute ( CNRRI ) , Hangzhou , China in 2001 . The experiments were carried out in two seasons followed a randomized complete block design .
[ Sen. 8, subscore: 1.00 ]: The parents and 304 F9 lines were grown in the paddy field in China National Rice Research Institute ( CNRRI ) , Hangzhou , China in 2001 . The experiments were carried out in two seasons followed a randomized complete block design . QTLMapper 1 . 01 was applied to detect QTLs and QTL x environment ( QE ) interaction for HD ( heading data ) , PH ( plant height ) and PN ( panicle number ) , and conditional mapping for PH and PN was performed as well . A total of 15 QTLs with significant additive effects were detected , among which 4 QTLs had significant QE interaction . Ten QTLs with additive x additive epistatic effects for PH , HD and PN were detected , among which none showed significant epistatisis x environment interaction . These QTLs explained 12 . 12% , 1 . 38% and 5 . 00% of the total phenotypic variance for PH , HD and PN , respectively , and contributions were generally lower due to the strong epistatic effects . In conditional QTL analysis , the numbers of QTLs showing significant additive and epistatic effects were 7 and 6 for PH , and 3 and 3 for PN , respectively . Among the QTLs having significant additive effects for PH , qPH7-2 showed both additive effects and QE interaction , qPH7-1 and qPH10 showed QE interaction only , and the remaining 4 QTLs showed additive effects only . Each of the 3 QTLs having significant additive effects for PN did not display significant QE interaction .
[ Sen. 9, subscore: 1.00 ]: The experiments were carried out in two seasons followed a randomized complete block design . QTLMapper 1 . 01 was applied to detect QTLs and QTL x environment ( QE ) interaction for HD ( heading data ) , PH ( plant height ) and PN ( panicle number ) , and conditional mapping for PH and PN was performed as well . A total of 15 QTLs with significant additive effects were detected , among which 4 QTLs had significant QE interaction . Ten QTLs with additive x additive epistatic effects for PH , HD and PN were detected , among which none showed significant epistatisis x environment interaction . These QTLs explained 12 . 12% , 1 . 38% and 5 . 00% of the total phenotypic variance for PH , HD and PN , respectively , and contributions were generally lower due to the strong epistatic effects . In conditional QTL analysis , the numbers of QTLs showing significant additive and epistatic effects were 7 and 6 for PH , and 3 and 3 for PN , respectively . Among the QTLs having significant additive effects for PH , qPH7-2 showed both additive effects and QE interaction , qPH7-1 and qPH10 showed QE interaction only , and the remaining 4 QTLs showed additive effects only . Each of the 3 QTLs having significant additive effects for PN did not display significant QE interaction . No epistatic QE interactions was detected .
[ Sen. 10, subscore: 1.00 ]: QTLMapper 1 . 01 was applied to detect QTLs and QTL x environment ( QE ) interaction for HD ( heading data ) , PH ( plant height ) and PN ( panicle number ) , and conditional mapping for PH and PN was performed as well . A total of 15 QTLs with significant additive effects were detected , among which 4 QTLs had significant QE interaction . Ten QTLs with additive x additive epistatic effects for PH , HD and PN were detected , among which none showed significant epistatisis x environment interaction . These QTLs explained 12 . 12% , 1 . 38% and 5 . 00% of the total phenotypic variance for PH , HD and PN , respectively , and contributions were generally lower due to the strong epistatic effects . In conditional QTL analysis , the numbers of QTLs showing significant additive and epistatic effects were 7 and 6 for PH , and 3 and 3 for PN , respectively . Among the QTLs having significant additive effects for PH , qPH7-2 showed both additive effects and QE interaction , qPH7-1 and qPH10 showed QE interaction only , and the remaining 4 QTLs showed additive effects only . Each of the 3 QTLs having significant additive effects for PN did not display significant QE interaction . No epistatic QE interactions was detected . In addition , conditional QTL analysis indicated that the expression of QTLs for PH and PN may vary depending on the QTLs for HD .
[ Sen. 12, subscore: 1.00 ]: Ten QTLs with additive x additive epistatic effects for PH , HD and PN were detected , among which none showed significant epistatisis x environment interaction . These QTLs explained 12 . 12% , 1 . 38% and 5 . 00% of the total phenotypic variance for PH , HD and PN , respectively , and contributions were generally lower due to the strong epistatic effects . In conditional QTL analysis , the numbers of QTLs showing significant additive and epistatic effects were 7 and 6 for PH , and 3 and 3 for PN , respectively . Among the QTLs having significant additive effects for PH , qPH7-2 showed both additive effects and QE interaction , qPH7-1 and qPH10 showed QE interaction only , and the remaining 4 QTLs showed additive effects only . Each of the 3 QTLs having significant additive effects for PN did not display significant QE interaction . No epistatic QE interactions was detected . In addition , conditional QTL analysis indicated that the expression of QTLs for PH and PN may vary depending on the QTLs for HD .
[ Sen. 14, subscore: 1.00 ]: In conditional QTL analysis , the numbers of QTLs showing significant additive and epistatic effects were 7 and 6 for PH , and 3 and 3 for PN , respectively . Among the QTLs having significant additive effects for PH , qPH7-2 showed both additive effects and QE interaction , qPH7-1 and qPH10 showed QE interaction only , and the remaining 4 QTLs showed additive effects only . Each of the 3 QTLs having significant additive effects for PN did not display significant QE interaction . No epistatic QE interactions was detected . In addition , conditional QTL analysis indicated that the expression of QTLs for PH and PN may vary depending on the QTLs for HD .
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