| 457 matches found in 226 documents. Search time: 0.013 seconds. |
|---|
|
Score: 6.00 |
|---|
| Title: In vivo and in vitro phosphorylation of rice dwarf phytoreovirus Pns12 cytoplasmic nonstructural protein .
| | Author: Suzuki N Hosokawa D Matsuura Y Kikuchi A Omura T | | Journal: Arch . Virol . Citation: V : 144 ( 7 ) P : 1371-80 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10481743 Accession (PMID): 10481743 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: In vivo and in vitro phosphorylation and intracellular location of rice dwarf phytoreovirus Pns12 , which is encoded by one of the twelve dsRNA genome segments , S12 , and comprises 312 amino acids , was investigated . When [ 32P ] phosphoric acid was incorporated into RDV-infected leafhopper cultured cells , labelled Pns12 was immunoprecipitated from the cells by a monospecific anti-Pns12 polyclonal antibody . Recombinant Pns12 was purified from Spodoptera frugiperda cells infected with AcRS12 , a baculovirus recombinant carrying a full-length cDNA of RDV S12 . Purified Pns12 was also demonstrated to be phosphorylated in vitro by a kinase activity present in host ( rice , barley , wheat , leafhopper ) cells and non-host ( tobacco , spinach , white clover , S frugiperda , mosquito , mammals ) cells as well . Immunocytochemical studies showed Pns12 accumulated in the cytoplasm of infected cells , and frequently localized in a slightly electron-dense patch .
[ Sen. 2, subscore: 1.00 ]: In vivo and in vitro phosphorylation and intracellular location of rice dwarf phytoreovirus Pns12 , which is encoded by one of the twelve dsRNA genome segments , S12 , and comprises 312 amino acids , was investigated . When [ 32P ] phosphoric acid was incorporated into RDV-infected leafhopper cultured cells , labelled Pns12 was immunoprecipitated from the cells by a monospecific anti-Pns12 polyclonal antibody . Recombinant Pns12 was purified from Spodoptera frugiperda cells infected with AcRS12 , a baculovirus recombinant carrying a full-length cDNA of RDV S12 . Purified Pns12 was also demonstrated to be phosphorylated in vitro by a kinase activity present in host ( rice , barley , wheat , leafhopper ) cells and non-host ( tobacco , spinach , white clover , S frugiperda , mosquito , mammals ) cells as well . Immunocytochemical studies showed Pns12 accumulated in the cytoplasm of infected cells , and frequently localized in a slightly electron-dense patch . These results demonstrated that RDV Pns12 was a cytoplasmic nonstructural phosphoprotein .
[ Sen. 3, subscore: 1.00 ]: In vivo and in vitro phosphorylation and intracellular location of rice dwarf phytoreovirus Pns12 , which is encoded by one of the twelve dsRNA genome segments , S12 , and comprises 312 amino acids , was investigated . When [ 32P ] phosphoric acid was incorporated into RDV-infected leafhopper cultured cells , labelled Pns12 was immunoprecipitated from the cells by a monospecific anti-Pns12 polyclonal antibody . Recombinant Pns12 was purified from Spodoptera frugiperda cells infected with AcRS12 , a baculovirus recombinant carrying a full-length cDNA of RDV S12 . Purified Pns12 was also demonstrated to be phosphorylated in vitro by a kinase activity present in host ( rice , barley , wheat , leafhopper ) cells and non-host ( tobacco , spinach , white clover , S frugiperda , mosquito , mammals ) cells as well . Immunocytochemical studies showed Pns12 accumulated in the cytoplasm of infected cells , and frequently localized in a slightly electron-dense patch . These results demonstrated that RDV Pns12 was a cytoplasmic nonstructural phosphoprotein .
[ Sen. 4, subscore: 1.00 ]: In vivo and in vitro phosphorylation and intracellular location of rice dwarf phytoreovirus Pns12 , which is encoded by one of the twelve dsRNA genome segments , S12 , and comprises 312 amino acids , was investigated . When [ 32P ] phosphoric acid was incorporated into RDV-infected leafhopper cultured cells , labelled Pns12 was immunoprecipitated from the cells by a monospecific anti-Pns12 polyclonal antibody . Recombinant Pns12 was purified from Spodoptera frugiperda cells infected with AcRS12 , a baculovirus recombinant carrying a full-length cDNA of RDV S12 . Purified Pns12 was also demonstrated to be phosphorylated in vitro by a kinase activity present in host ( rice , barley , wheat , leafhopper ) cells and non-host ( tobacco , spinach , white clover , S frugiperda , mosquito , mammals ) cells as well . Immunocytochemical studies showed Pns12 accumulated in the cytoplasm of infected cells , and frequently localized in a slightly electron-dense patch . These results demonstrated that RDV Pns12 was a cytoplasmic nonstructural phosphoprotein .
[ Sen. 5, subscore: 1.00 ]: In vivo and in vitro phosphorylation and intracellular location of rice dwarf phytoreovirus Pns12 , which is encoded by one of the twelve dsRNA genome segments , S12 , and comprises 312 amino acids , was investigated . When [ 32P ] phosphoric acid was incorporated into RDV-infected leafhopper cultured cells , labelled Pns12 was immunoprecipitated from the cells by a monospecific anti-Pns12 polyclonal antibody . Recombinant Pns12 was purified from Spodoptera frugiperda cells infected with AcRS12 , a baculovirus recombinant carrying a full-length cDNA of RDV S12 . Purified Pns12 was also demonstrated to be phosphorylated in vitro by a kinase activity present in host ( rice , barley , wheat , leafhopper ) cells and non-host ( tobacco , spinach , white clover , S frugiperda , mosquito , mammals ) cells as well . Immunocytochemical studies showed Pns12 accumulated in the cytoplasm of infected cells , and frequently localized in a slightly electron-dense patch . These results demonstrated that RDV Pns12 was a cytoplasmic nonstructural phosphoprotein .
[ Sen. 6, subscore: 1.00 ]: When [ 32P ] phosphoric acid was incorporated into RDV-infected leafhopper cultured cells , labelled Pns12 was immunoprecipitated from the cells by a monospecific anti-Pns12 polyclonal antibody . Recombinant Pns12 was purified from Spodoptera frugiperda cells infected with AcRS12 , a baculovirus recombinant carrying a full-length cDNA of RDV S12 . Purified Pns12 was also demonstrated to be phosphorylated in vitro by a kinase activity present in host ( rice , barley , wheat , leafhopper ) cells and non-host ( tobacco , spinach , white clover , S frugiperda , mosquito , mammals ) cells as well . Immunocytochemical studies showed Pns12 accumulated in the cytoplasm of infected cells , and frequently localized in a slightly electron-dense patch . These results demonstrated that RDV Pns12 was a cytoplasmic nonstructural phosphoprotein .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
|---|
| Title: Glutelin basic subunits have a mammalian mucin-type O-linked disaccharide side chain .
| | Author: Kishimoto T Watanabe M Mitsui T Hori H | | Journal: Arch . Biochem . Biophys . Citation: V : 370 ( 2 ) P : 271-7 Year: 1999 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10510286 Accession (PMID): 10510286 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Characterization was done on the sugar chains of glutelin , a major storage protein in rice seeds . The basic subunits of glutelin were shown to be glycoproteins by staining with PAS and the lectins concanavalin A ( Con A ) and peanut agglutinin ( PNA ) . The binding of PNA to the basic subunits was substantially reduced by treatment of the glutelin with NaIO4 , NaOH , or O-glycanase . The sugar chains of the subunits , obtained by hydrazinolysis and O-glycanase , were pyridylaminated and subjected to 2-dimensional HPLC analysis using C ( 18 ) and acrylamide-derivatized columns . It was found that the Galbeta-1 , 3GalNAc disaccharide , which was previously identified as a core 1 structure of mucin-type sugar chains , is conjugated to the glutelin subunits . Furthermore , amino acid sequencing of an 11-kDa peptide of the subunits , recognized by both PNA and Con A , suggested that both N-linked and O-linked glycosylation occurs in the carboxy-terminal region of these subunits .
[ Sen. 3, subscore: 1.00 ]: Characterization was done on the sugar chains of glutelin , a major storage protein in rice seeds . The basic subunits of glutelin were shown to be glycoproteins by staining with PAS and the lectins concanavalin A ( Con A ) and peanut agglutinin ( PNA ) . The binding of PNA to the basic subunits was substantially reduced by treatment of the glutelin with NaIO4 , NaOH , or O-glycanase . The sugar chains of the subunits , obtained by hydrazinolysis and O-glycanase , were pyridylaminated and subjected to 2-dimensional HPLC analysis using C ( 18 ) and acrylamide-derivatized columns . It was found that the Galbeta-1 , 3GalNAc disaccharide , which was previously identified as a core 1 structure of mucin-type sugar chains , is conjugated to the glutelin subunits . Furthermore , amino acid sequencing of an 11-kDa peptide of the subunits , recognized by both PNA and Con A , suggested that both N-linked and O-linked glycosylation occurs in the carboxy-terminal region of these subunits .
[ Sen. 6, subscore: 1.00 ]: The basic subunits of glutelin were shown to be glycoproteins by staining with PAS and the lectins concanavalin A ( Con A ) and peanut agglutinin ( PNA ) . The binding of PNA to the basic subunits was substantially reduced by treatment of the glutelin with NaIO4 , NaOH , or O-glycanase . The sugar chains of the subunits , obtained by hydrazinolysis and O-glycanase , were pyridylaminated and subjected to 2-dimensional HPLC analysis using C ( 18 ) and acrylamide-derivatized columns . It was found that the Galbeta-1 , 3GalNAc disaccharide , which was previously identified as a core 1 structure of mucin-type sugar chains , is conjugated to the glutelin subunits . Furthermore , amino acid sequencing of an 11-kDa peptide of the subunits , recognized by both PNA and Con A , suggested that both N-linked and O-linked glycosylation occurs in the carboxy-terminal region of these subunits .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 6.00 |
|---|
| Title: Developmentally regulated expression of a peptide : N-glycanase during germination of rice seeds ( Oryza sativa ) and its purification and characterization .
| | Author: Chang T Kuo MC Khoo KH Inoue S Inoue Y | | Journal: J Biol . Chem . Citation: V : 275 ( 1 ) P : 129-34 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10617595 Accession (PMID): 10617595 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Peptide : N-glycanase ( PNGase ; EC 3 . 5 . 1 . 52 ) activity was detected in dormant rice seeds ( Oryza sativa ) and the imbibed rice grains . Time-course studies revealed that the enzyme activity remained almost constant until about 30 h after imbibition in both of endosperm and embryo it issue-containing areas , and started to increase only in growing germ part , reached a peak at about 3-day stage , followed by a gradual decrease concomitant with a sharp increase in the coleoptile . The specific activity increased about 6-fold at about 3-day stage . PNGase was purified to electrophoretic homogeneity from the extracts of germinated rice seeds at 24 h , and the apparent molecular weight of the purified enzyme , estimated by SDS-polyacrylamide gel electrophoresis ( SDS-PAGE ) , was about 80 , 000 . The purified enzyme was designated PNGase Os to denote its origin .
[ Sen. 4, subscore: 1.00 ]: Peptide : N-glycanase ( PNGase ; EC 3 . 5 . 1 . 52 ) activity was detected in dormant rice seeds ( Oryza sativa ) and the imbibed rice grains . Time-course studies revealed that the enzyme activity remained almost constant until about 30 h after imbibition in both of endosperm and embryo it issue-containing areas , and started to increase only in growing germ part , reached a peak at about 3-day stage , followed by a gradual decrease concomitant with a sharp increase in the coleoptile . The specific activity increased about 6-fold at about 3-day stage . PNGase was purified to electrophoretic homogeneity from the extracts of germinated rice seeds at 24 h , and the apparent molecular weight of the purified enzyme , estimated by SDS-polyacrylamide gel electrophoresis ( SDS-PAGE ) , was about 80 , 000 . The purified enzyme was designated PNGase Os to denote its origin . The N-terminal sequence of the 10 residues was determined to be SYNVASVAGL . The purified PNGase Os in SDS-PAGE appeared as a rather broad band , consistent with the presence of multiple glycoforms as indicated by chromatographic behavior on a Sephadex G-75 column . PNGase expressed in coleoptile under anoxia condition was also purified , and both of the purified enzymes were found to exhibit very similar , if not identical , electrophoretic mobility in SDS-PAGE .
[ Sen. 5, subscore: 1.00 ]: Peptide : N-glycanase ( PNGase ; EC 3 . 5 . 1 . 52 ) activity was detected in dormant rice seeds ( Oryza sativa ) and the imbibed rice grains . Time-course studies revealed that the enzyme activity remained almost constant until about 30 h after imbibition in both of endosperm and embryo it issue-containing areas , and started to increase only in growing germ part , reached a peak at about 3-day stage , followed by a gradual decrease concomitant with a sharp increase in the coleoptile . The specific activity increased about 6-fold at about 3-day stage . PNGase was purified to electrophoretic homogeneity from the extracts of germinated rice seeds at 24 h , and the apparent molecular weight of the purified enzyme , estimated by SDS-polyacrylamide gel electrophoresis ( SDS-PAGE ) , was about 80 , 000 . The purified enzyme was designated PNGase Os to denote its origin . The N-terminal sequence of the 10 residues was determined to be SYNVASVAGL . The purified PNGase Os in SDS-PAGE appeared as a rather broad band , consistent with the presence of multiple glycoforms as indicated by chromatographic behavior on a Sephadex G-75 column . PNGase expressed in coleoptile under anoxia condition was also purified , and both of the purified enzymes were found to exhibit very similar , if not identical , electrophoretic mobility in SDS-PAGE . PNGase Os exhibited a broad pH-activity profile with an optimum of 4-5 and , interestingly , was significantly inactivated by K ( + ) and Na ( + ) at near the physiological concentration , 100 mM .
[ Sen. 7, subscore: 1.00 ]: The specific activity increased about 6-fold at about 3-day stage . PNGase was purified to electrophoretic homogeneity from the extracts of germinated rice seeds at 24 h , and the apparent molecular weight of the purified enzyme , estimated by SDS-polyacrylamide gel electrophoresis ( SDS-PAGE ) , was about 80 , 000 . The purified enzyme was designated PNGase Os to denote its origin . The N-terminal sequence of the 10 residues was determined to be SYNVASVAGL . The purified PNGase Os in SDS-PAGE appeared as a rather broad band , consistent with the presence of multiple glycoforms as indicated by chromatographic behavior on a Sephadex G-75 column . PNGase expressed in coleoptile under anoxia condition was also purified , and both of the purified enzymes were found to exhibit very similar , if not identical , electrophoretic mobility in SDS-PAGE . PNGase Os exhibited a broad pH-activity profile with an optimum of 4-5 and , interestingly , was significantly inactivated by K ( + ) and Na ( + ) at near the physiological concentration , 100 mM . These results are discussed in relation to other work .
[ Sen. 8, subscore: 1.00 ]: PNGase was purified to electrophoretic homogeneity from the extracts of germinated rice seeds at 24 h , and the apparent molecular weight of the purified enzyme , estimated by SDS-polyacrylamide gel electrophoresis ( SDS-PAGE ) , was about 80 , 000 . The purified enzyme was designated PNGase Os to denote its origin . The N-terminal sequence of the 10 residues was determined to be SYNVASVAGL . The purified PNGase Os in SDS-PAGE appeared as a rather broad band , consistent with the presence of multiple glycoforms as indicated by chromatographic behavior on a Sephadex G-75 column . PNGase expressed in coleoptile under anoxia condition was also purified , and both of the purified enzymes were found to exhibit very similar , if not identical , electrophoretic mobility in SDS-PAGE . PNGase Os exhibited a broad pH-activity profile with an optimum of 4-5 and , interestingly , was significantly inactivated by K ( + ) and Na ( + ) at near the physiological concentration , 100 mM . These results are discussed in relation to other work .
[ Sen. 9, subscore: 1.00 ]: The purified enzyme was designated PNGase Os to denote its origin . The N-terminal sequence of the 10 residues was determined to be SYNVASVAGL . The purified PNGase Os in SDS-PAGE appeared as a rather broad band , consistent with the presence of multiple glycoforms as indicated by chromatographic behavior on a Sephadex G-75 column . PNGase expressed in coleoptile under anoxia condition was also purified , and both of the purified enzymes were found to exhibit very similar , if not identical , electrophoretic mobility in SDS-PAGE . PNGase Os exhibited a broad pH-activity profile with an optimum of 4-5 and , interestingly , was significantly inactivated by K ( + ) and Na ( + ) at near the physiological concentration , 100 mM . These results are discussed in relation to other work .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
|---|
| Title: Genotypic characterization of drug-resistant Mycobacterium tuberculosis isolates from Peru .
| | Author: Escalante P Ramaswamy S Sanabria H Soini H Pan X Valiente-Castillo O Musser JM .
| | Journal: Tuber .
Lung Dis .
Citation: V : 79 ( 2 ) P : 111-8 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10645449 Accession (PMID): 10645449 | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: SETTING : Twenty-nine epidemiological unrelated and mostly multidrug-resistant Mycobacterium tuberculosis ( MDR-TB ) strains from Peruvian patients . OBJECTIVE : To investigate the molecular genetics of MDR-TB strains recovered in a Latin American country . DESIGN : Antimicrobial agent susceptibility testing , major genetic group designation , IS6110 fingerprinting , spoligotyping , and automated deoxyribonucleic acid sequencing of regions of the katG , rpoB , embB , gyrA , and pncA genes with mutations commonly associated with drug resistance . RESULTS : Nineteen isolates were found to be multidrug-resistant by susceptibility testing . IS6110 typing showed that virtually all isolates were unique and therefore had independently acquired drug resistance . Seventy-nine percent of isoniazid-resistant strains had a Ser315Thr amino acid change in KatG . Ninety-five percent of rifampin-resistant isolates had amino acid replacements in the rifampin-resistance determining region of RpoB .
[ Sen. 9, subscore: 1.00 ]: IS6110 typing showed that virtually all isolates were unique and therefore had independently acquired drug resistance . Seventy-nine percent of isoniazid-resistant strains had a Ser315Thr amino acid change in KatG . Ninety-five percent of rifampin-resistant isolates had amino acid replacements in the rifampin-resistance determining region of RpoB . Six of 11 ethambutol-resistant strains had EmbB alterations . Eleven pyrazinamide-resistant strains had distinct mutations in pncA . CONCLUSION : Virtually all organisms evolved drug resistance independently . The types of drug resistance-associated mutations identified were very similar to changes occurring in isolates from other areas of the world . Nucleotide sequence-based strategies for rapid detection of drug resistance-conferring mutants will be applicable to organisms recovered in Peru , and potentially other areas of Latin America .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
|---|
| Title: Sequence analysis of Pns11 , a nonstructural protein of rice gall dwarf virus , and its expression and detection in infected rice plants and vector insects .
| | Author: Moriyasu Y Ishikawa K Kikuchi A Imanishi S Tomita S Akutsu K Omura T | | Journal: Virus Genes Citation: V : 20 ( 3 ) P : 237-41 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10949951 Accession (PMID): 10949951 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: The nucleotide sequence of genome segment S11 of rice gall dwarf virus ( RGDV ) , a member of Phytoreovirus , was determined . The segment encodes a putative protein of 40 kDa that exhibits approximately 37% homology at the amino acid level to the nonstructural proteins Pns10 of rice dwarf and wound tumor viruses , which are other members of Phytoreovirus . A band of a protein with an apparent molecular mass of 40 kDa was specifically detected in an analysis of cells transfected with S11 cDNA . An antiserum raised against this protein reacted with a protein of approximately 40kDa after fractionation by SDS-PAGE of materials prepared from infected plants and from viruliferous vector insects . However , the antiserum did not react with purified viral proteins . These results suggest that S11 encodes a nonstructural protein of RGDV .
[ Sen. 7, subscore: 1.00 ]: A band of a protein with an apparent molecular mass of 40 kDa was specifically detected in an analysis of cells transfected with S11 cDNA . An antiserum raised against this protein reacted with a protein of approximately 40kDa after fractionation by SDS-PAGE of materials prepared from infected plants and from viruliferous vector insects . However , the antiserum did not react with purified viral proteins . These results suggest that S11 encodes a nonstructural protein of RGDV . This protein was named Pns11 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
|---|
| Title: Sequencing of the rpoB gene in Legionella pneumophila and characterization of mutations associated with rifampin resistance in the Legionellaceae .
| | Author: Nielsen K Hindersson P Hoiby N Bangsborg JM .
| | Journal: Antimicrob .
Agents Chemother .
Citation: V : 44 ( 10 ) P : 2679-83 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10991843 Accession (PMID): 10991843 | Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: Rifampin in combination with erythromycin is a recommended treatment for severe cases of legionellosis . Mutations in the rpoB gene are known to cause rifampin resistance in Escherichia coli and Mycobacterium tuberculosis , and the purpose of the present study was to investigate a possible similar resistance mechanism within the members of the family Legionellaceae . Since the RNA polymerase genes of this genus have never been characterized , the DNA sequence of the Legionella pneumophila rpoB gene was determined by the Vectorette technique for genome walking . A 4 , 647-bp DNA sequence that contained the open reading frame ( ORF ) of the rpoB gene ( 4 , 104 bp ) and an ORF of 384 bp representing part of the rpoC gene was obtained . A 316-bp DNA fragment in the center of the L pneumophila rpoB gene , corresponding to a previously described site for mutations leading to rifampin resistance in M tuberculosis , was sequenced from 18 rifampin-resistant Legionella isolates representing four species ( L bozemanii , L longbeachae , L micdadei , and L pneumophila ) , and the sequences were compared to the sequences of the fragments from the parent ( rifampin-sensitive ) strains . Six single-base mutations which led to amino acid substitutions at five different positions were identified . A single strain did not contain any mutations in the 316-bp fragment . This study represents the characterization of a hitherto undescribed resistance mechanism within the family Legionellaceae .
[ Sen. 3, subscore: 1.00 ]: Rifampin in combination with erythromycin is a recommended treatment for severe cases of legionellosis . Mutations in the rpoB gene are known to cause rifampin resistance in Escherichia coli and Mycobacterium tuberculosis , and the purpose of the present study was to investigate a possible similar resistance mechanism within the members of the family Legionellaceae . Since the RNA polymerase genes of this genus have never been characterized , the DNA sequence of the Legionella pneumophila rpoB gene was determined by the Vectorette technique for genome walking . A 4 , 647-bp DNA sequence that contained the open reading frame ( ORF ) of the rpoB gene ( 4 , 104 bp ) and an ORF of 384 bp representing part of the rpoC gene was obtained . A 316-bp DNA fragment in the center of the L pneumophila rpoB gene , corresponding to a previously described site for mutations leading to rifampin resistance in M tuberculosis , was sequenced from 18 rifampin-resistant Legionella isolates representing four species ( L bozemanii , L longbeachae , L micdadei , and L pneumophila ) , and the sequences were compared to the sequences of the fragments from the parent ( rifampin-sensitive ) strains . Six single-base mutations which led to amino acid substitutions at five different positions were identified . A single strain did not contain any mutations in the 316-bp fragment .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Genotypic determination of Mycobacterium tuberculosis antibiotic resistance using a novel mutation detection method , the branch migration inhibition M tuberculosis antibiotic resistance test | | Author: Liu YP Behr MA Small PM Kurn N | | Journal: J Clin . Microbiol . Citation: V : 38 ( 10 ) P : 3656-62 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11015379 Accession (PMID): 11015379 | Matching Sentences:
[ Sen. 7, subscore: 1.00 ]: Essential to this method are the generation of partial duplexes that are capable of forming four-stranded structures and the ability to detect inhibition of branch migration in these structures ( I G Panyutin and P Hsieh , J Mol . Biol . 230 : 413-424 , 1993 ) . Inhibition of branch migration indicates the presence of sequence alteration . This mutation detection method , termed branch migration inhibition ( BMI ) , is suitable for the detection of drug resistance in M tuberculosis , which is frequently associated with multiple mutations within known genes . We describe the genotypic determination of the rifampin ( RMP ) and pyrazinamide ( PZA ) susceptibilities of M tuberculosis isolates , using BMI coupled with the luminescence oxygen channeling immunoassay ( LOCI ) ( E F Ullman et al , Proc . Natl . Acad . Sci . USA 91 : 5426-5430 , 1994 ) . RMP and PZA resistances are associated with multiple mutations within the rpoB and pncA genes , respectively . M tuberculosis genomic DNA samples prepared from 46 clinical isolates were used for genotypic determination of RMP resistance in a "blind study . " Similarly , PZA resistance was determined using genomic DNA samples prepared from 37 clinical isolates . Full agreement of the genotypic and phenotypic determinations of drug susceptibility was demonstrated . RMP susceptibility determination directly from cells of 10 clinical isolates grown in culture was also demonstrated . The genotypic result of only 1 out of 10 isolates did not agree with the phenotypic susceptibility testing result .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
|---|
| Title: [ A patient with dysphagia treated successfully and discharged without nutritional support ] | | Author: Okuyama Y Nonomura Y Hatanaka N | | Journal: Gan To Kagaku Ryoho Citation: V : 27 Suppl 3 ( ) P : 754-5 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11190340 Accession (PMID): 11190340 | Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: Intravenous hyperalimentation and tube-feeding at home are suitable solutions for some patients with dysphagia after cerebral infarction . However , the difficulties faced in their management are the burden on the families , which tends to be an obstacle for at-home-practice . We describe herein a case of severe dysphagia treated successfully through our rehabilitation program and discharged without nutritional supports . An 82-year-old man was admitted to our hospital suffering from pyrexia and dysbasia . The man , who lives with his wife and his sons family , was diagnosed with aspiration pneumonia and multiple cerebral infarctions . The test for swallowing reflex revealed an impaired first phase reflex and intravenous hyperalimentation was performed for his nutritional support . He was still suffering from dysphagia but had the desire to eat orally after his dysbasia and aspiration pneumonia were cured . A rehabilitation program was scheduled with the aims of 1 ) recovery of ingestion and 2 ) sufficient expectoration , with an ongoing teaching program for the management of intravenous hyperalimentation . After one month of rehabilitation ( ice-massaging , muscle rehabilitation of the tongue and neck and expectoration training in a prone position and after gorging ) , his ability to swallow was gradually recovered .
[ Sen. 8, subscore: 1.00 ]: We describe herein a case of severe dysphagia treated successfully through our rehabilitation program and discharged without nutritional supports . An 82-year-old man was admitted to our hospital suffering from pyrexia and dysbasia . The man , who lives with his wife and his sons family , was diagnosed with aspiration pneumonia and multiple cerebral infarctions . The test for swallowing reflex revealed an impaired first phase reflex and intravenous hyperalimentation was performed for his nutritional support . He was still suffering from dysphagia but had the desire to eat orally after his dysbasia and aspiration pneumonia were cured . A rehabilitation program was scheduled with the aims of 1 ) recovery of ingestion and 2 ) sufficient expectoration , with an ongoing teaching program for the management of intravenous hyperalimentation . After one month of rehabilitation ( ice-massaging , muscle rehabilitation of the tongue and neck and expectoration training in a prone position and after gorging ) , his ability to swallow was gradually recovered . With the frequent confirmation of absence of aspiration , special forms of diets were served and upgraded from jelly , paste-like-food to soft-cooked steamed rice . The patient is now at home without any nutritional support .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
|---|
| Title: Transposon impala , a novel tool for gene tagging in the rice blast fungus Magnaporthe grisea .
| | Author: Villalba F Lebrun MH Hua-Van A Daboussi MJ Grosjean-Cournoyer MC .
| | Journal: Mol .
Plant Microbe Interact .
Citation: V : 14 ( 3 ) P : 308-15 Year: 2001 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11277428 Accession (PMID): 11277428 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: impala , a Tc1-mariner transposable element from Fusarium oxysporum , was introduced into the rice blast fungus Magnaporthe grisea to develop transposon-based insertional mutagenesis . A construct ( pNIL160 ) containing an autonomous impala copy inserted in the promoter of niaD encoding Aspergillus nidulans nitrate reductase was introduced by transformation into a M grisea nitrate reductase-deficient mutant . impala excision was monitored by restoration of prototrophy for nitrate . Southern analysis of niaD+ revertants revealed that impala was able to excise and reinsert at new loci in M grisea . As observed for its host Fusarium oxysporum , impala inserted at a TA site left a typical excision footprint of 5 bp . We have shown that a defective impala copy was inactive in M grisea , yet it can be activated by a functional impala transposase . A transformant carrying a single copy of pNIL160 was used to generate a collection of 350 revertants .
[ Sen. 6, subscore: 1.00 ]: A construct ( pNIL160 ) containing an autonomous impala copy inserted in the promoter of niaD encoding Aspergillus nidulans nitrate reductase was introduced by transformation into a M grisea nitrate reductase-deficient mutant . impala excision was monitored by restoration of prototrophy for nitrate . Southern analysis of niaD+ revertants revealed that impala was able to excise and reinsert at new loci in M grisea . As observed for its host Fusarium oxysporum , impala inserted at a TA site left a typical excision footprint of 5 bp . We have shown that a defective impala copy was inactive in M grisea , yet it can be activated by a functional impala transposase . A transformant carrying a single copy of pNIL160 was used to generate a collection of 350 revertants . Mutants either altered for their mycelial growth ( Rev2 ) or nonpathogenic ( Rev77 ) were obtained . Complementation of Rev77 with a 3-kb genomic fragment from a wild-type locus was successful , demonstrating the tagging of a pathogenicity gene by impala . This gene , called ORP1 , is essential for penetration of host leaves by M grisea and has no sequence homology to known genes .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Rice alpha-mannosidase digesting the high mannose glycopeptide of glutelin .
| | Author: Kishimoto T Hori H Takano D Nakano Y Watanabe M Mitsui T | | Journal: Citation: V : 112 ( 1 ) P : 15-24 Year: 2001 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11319010 Accession (PMID): 11319010 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: alpha-Mannosidase ( EC 3 . 2 . 1 . 24 ) from rice dry seeds was purified to homogeneity . Optimum pH and Km for pNP-alpha-Man hydrolysis were pH 4 . 3-4 . 5 and 1 . 04 mM , respectively . The enzyme digested mannobioses such as Manalpha-1 , 2Man , Manalpha-1 , 6Man , Manalpha-1 , 3Man but Manalpha-1 , 4Man . Zn2+ ion was required for the activity , whereas EDTA and swainsonine inhibited the activity by 80 and 96% , respectively . The rice storage protein , glutelin was prepared and its basic subunits were shown to have high mannose-type sugar chains by two-dimensional mapping using NH2-P and C18 silica columns . They were Man9GlcNAc2 , Man8GlcNAc2 , Man7GlcNAc2 , Man6GlcNAc2 and Man5GlcNAc2 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Risk factors associated with leptospirosis in northeastern Thailand , 1998 .
| | Author: Tangkanakul W Tharmaphornpil P Plikaytis BD Bragg S Poonsuksombat D Choomkasien P Kingnate D Ashford DA .
| | Journal: Am .
J Trop . Med . Hyg . Citation: V : 63 ( 3-4 ) P : 204-8 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11388516 Accession (PMID): 11388516 | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: Leptospirosis is a zoonotic disease of worldwide distribution caused by spirochetes of the genus Leptospira . Humans are infected through direct contact with infected animals or through exposure to fresh water or soil contaminated by infected animal urine . Leptospirosis is characterized by acute fever that can be followed by a more severe , sometimes fatal illness that may include jaundice and renal failure ( Weils disease ) , meningitis , myocarditis , hemorrhagic pneumonitis , or hemodynamic collapse . To identify potential risk factors for leptospirosis in Thailand , we conducted a matched case-control study in Nakornratchasrima Province of the northeastern region . Fifty-nine cases and 118 controls were included in the study . Four activities in the two weeks prior to illness were independently associated with leptospirosis infection : walking through water ( odds ratio [ OR ] = 4 . 9 , 95% confidence interval [ CI ] = 1 . 7-14 . 1 ) , applying fertilizer in wet fields for more than 6 hr a day ( OR = 3 . 4 , 95% CI = 1 . 5-7 . 8 ) , plowing in wet fields for more than 6 hr a day ( OR = 3 . 5 , 95% CI = 1 . 1-11 . 6 ) , and pulling out rice plant sprouts in wet fields for more than 6 hr a day ( OR = 3 . 1 , 95% CI = 1 . 02-9 . 3 ) . Identification of these risk factors on admission might prove useful for early diagnosis and treatment of leptospirosis in Thailand .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 4.00 |
|---|
| Title: [ QTL dissection of panicle number per plant and spikelet number per panicle in rice ( Oryza sativa L ) ] | | Author: Xu JL Xue QZ Luo LJ Li ZK .
| | Journal: Yi Chuan Xue Bao Citation: V : 28 ( 8 ) P : 752-9 Year: 2001 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11554350 Accession (PMID): 11554350 | Matching Sentences:
[ Sen. 7, subscore: 2.00 ]: The weak negative correlation between PN and SNP was observed . Fifty-one QTLs and 45 epistatic QTL pairs affecting these traits were identified , collectively explaining over 60% of the total variation of individual traits . Almost all SNP-QTLs were attributable to one or more of its contributing components . Branching number traits had greater contributions to SNP than length traits , in which the first had twice as many QTLs mapped in the same or near regions with SNP as the latter . Only two PN-QTLs were mapped in the near regions with those of related traits of SNP , suggesting a reasonable recombination between PN and SNP would be available by marker-assisted selection ( MAS ) . Some major QTLs including QPn4 for panicle number , QPbn3a , QPbn3b and QPbl4 for panicle branching and length would be of great value in MAS . It was discussed that a new high-yielding panicle type was resulted from reasonably deploying for QTLs of panicle traits by MAS .
[ Sen. 1, subscore: 1.00 ]: The genetic mechanism underlying panicle number per plant ( PN ) , spikelet number per panicle ( SNP ) and its related traits in rice was analysed using 292 F13 RILs from the cross of Lemont/Teqing and a complete linkage map with 272 molecular markers . The RILs showed tremendous transgressive segregation for all traits studied . The weak negative correlation between PN and SNP was observed . Fifty-one QTLs and 45 epistatic QTL pairs affecting these traits were identified , collectively explaining over 60% of the total variation of individual traits . Almost all SNP-QTLs were attributable to one or more of its contributing components .
[ Sen. 3, subscore: 1.00 ]: The genetic mechanism underlying panicle number per plant ( PN ) , spikelet number per panicle ( SNP ) and its related traits in rice was analysed using 292 F13 RILs from the cross of Lemont/Teqing and a complete linkage map with 272 molecular markers . The RILs showed tremendous transgressive segregation for all traits studied . The weak negative correlation between PN and SNP was observed . Fifty-one QTLs and 45 epistatic QTL pairs affecting these traits were identified , collectively explaining over 60% of the total variation of individual traits . Almost all SNP-QTLs were attributable to one or more of its contributing components . Branching number traits had greater contributions to SNP than length traits , in which the first had twice as many QTLs mapped in the same or near regions with SNP as the latter . Only two PN-QTLs were mapped in the near regions with those of related traits of SNP , suggesting a reasonable recombination between PN and SNP would be available by marker-assisted selection ( MAS ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Isolation and characterization of triacontanol-regulated genes in rice ( Oryza sativa L ) : possible role of triacontanol as a plant growth stimulator .
| | Author: Chen X Yuan H Chen R Zhu L Du B Weng Q He G | | Journal: Plant Cell Physiol .
Citation: V : 43 ( 8 ) P : 869-76 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12198189 Accession (PMID): 12198189 | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: Triacontanol ( TRIA ) is a saturated long-chain alcohol that is known to have a growth promoting activity when exogenously supplied to a number of plants . In this study , dry weight , protein and chlorophyll contents of rice seedlings were increased by foliar application of TRIA . Leaf net photosynthesis rate ( Pn ) was increased very quickly and persistently at a given photon flux density ( PFD ) . The TRIA-regulated genes in rice were isolated from cDNA library by differential screening with probes generated from the forward and reverse-suppression subtractive hybridization ( SSH ) populations and confirmed by Northern blot . Sequence analysis revealed that most of the up-regulated genes encoded the photosynthetic and photorespiratory proteins . Two down-regulated genes were identified as those encoding an ABA and stress-related protein and a wounding-related protein . These results suggested that TRIA up-regulated the photosynthesis process and suppressed stresses in rice plants .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 11.00 |
|---|
| Title: Purification and characterization of beta-N-acetylhexosaminidase from rice seeds .
| | Author: Jin YL Jo YY Kim KY Shim JH Kim YW Park RD .
| | Journal: J Biochem . Mol . Biol . Citation: V : 35 ( 3 ) P : 313-9 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12297015 Accession (PMID): 12297015 | Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: Dongjin ) using ammonium sulfate ( 80% ) precipitation , Sephadex G-150 , CM-Sephadex , and DEAE-Sephadex chromatography , sequentially . The activities were separated into 7 fractions ( Fsub1 ; - F7sub7 ) by CM-Sephadex chromatography . Among them , F6 was further purified to homogeneity with a 13 . 0% yield and 123 . 3 purification-fold . The molecular mass was estimated to be about 52 kDa on SDS-PAGE and 37 . 4 kDa on Sephacryl S 300 gel filtration . The enzyme catalyzed the hydrolysis of both p-nitrophenyl-N-acetyl-beta-D-glucosaminide ( pNP-GlcNAc ) and p-nitrophenyl-N-acetyl-beta-D-galactosaminide ( pNPGalNAc ) as substrates , which are typical properties of beta-HexNAcase . The ratio of the pNP-GlcNAcase activity to the pNP-GalNAcase activity was 4 . 0 . However , it could not hydrolyze chitin , chitosan , pNP-beta-glucopyranoside , or pNP-beta-galactopyranoside . The enzyme showed K ( M ) , V ( max ) and K ( cat ) for pNP-GlcNAc of 1 . 65mM , 79 . 49mM min ( 1 ) , and 4 . 79 x 10 ( 6 ) min ( 1 ) , respectively . The comparison of kinetic values for pNPGlcNAc and pNP-GalNAc revealed that the two enzyme activities are associated with a single binding site .
[ Sen. 7, subscore: 2.00 ]: The activities were separated into 7 fractions ( Fsub1 ; - F7sub7 ) by CM-Sephadex chromatography . Among them , F6 was further purified to homogeneity with a 13 . 0% yield and 123 . 3 purification-fold . The molecular mass was estimated to be about 52 kDa on SDS-PAGE and 37 . 4 kDa on Sephacryl S 300 gel filtration . The enzyme catalyzed the hydrolysis of both p-nitrophenyl-N-acetyl-beta-D-glucosaminide ( pNP-GlcNAc ) and p-nitrophenyl-N-acetyl-beta-D-galactosaminide ( pNPGalNAc ) as substrates , which are typical properties of beta-HexNAcase . The ratio of the pNP-GlcNAcase activity to the pNP-GalNAcase activity was 4 . 0 . However , it could not hydrolyze chitin , chitosan , pNP-beta-glucopyranoside , or pNP-beta-galactopyranoside . The enzyme showed K ( M ) , V ( max ) and K ( cat ) for pNP-GlcNAc of 1 . 65mM , 79 . 49mM min ( 1 ) , and 4 . 79 x 10 ( 6 ) min ( 1 ) , respectively . The comparison of kinetic values for pNPGlcNAc and pNP-GalNAc revealed that the two enzyme activities are associated with a single binding site . The purified enzyme exhibited optimum pH and temperature for pNPGlcNAc of 5 . 0 and 50 degrees C , respectively .
[ Sen. 8, subscore: 2.00 ]: Among them , F6 was further purified to homogeneity with a 13 . 0% yield and 123 . 3 purification-fold . The molecular mass was estimated to be about 52 kDa on SDS-PAGE and 37 . 4 kDa on Sephacryl S 300 gel filtration . The enzyme catalyzed the hydrolysis of both p-nitrophenyl-N-acetyl-beta-D-glucosaminide ( pNP-GlcNAc ) and p-nitrophenyl-N-acetyl-beta-D-galactosaminide ( pNPGalNAc ) as substrates , which are typical properties of beta-HexNAcase . The ratio of the pNP-GlcNAcase activity to the pNP-GalNAcase activity was 4 . 0 . However , it could not hydrolyze chitin , chitosan , pNP-beta-glucopyranoside , or pNP-beta-galactopyranoside . The enzyme showed K ( M ) , V ( max ) and K ( cat ) for pNP-GlcNAc of 1 . 65mM , 79 . 49mM min ( 1 ) , and 4 . 79 x 10 ( 6 ) min ( 1 ) , respectively . The comparison of kinetic values for pNPGlcNAc and pNP-GalNAc revealed that the two enzyme activities are associated with a single binding site . The purified enzyme exhibited optimum pH and temperature for pNPGlcNAc of 5 . 0 and 50 degrees C , respectively . The enzyme activity for pNP-GlcNAc was stable at pH 5 . 0-5 . 5 and 20-40 degrees C The enzyme activity was completely inhibited at a concentration of 0 . 1 mM HgCl ( 2 ) and AgNO ( 3 ) , suggesting that the intact thiol group is essential for activity .
[ Sen. 10, subscore: 2.00 ]: The enzyme catalyzed the hydrolysis of both p-nitrophenyl-N-acetyl-beta-D-glucosaminide ( pNP-GlcNAc ) and p-nitrophenyl-N-acetyl-beta-D-galactosaminide ( pNPGalNAc ) as substrates , which are typical properties of beta-HexNAcase . The ratio of the pNP-GlcNAcase activity to the pNP-GalNAcase activity was 4 . 0 . However , it could not hydrolyze chitin , chitosan , pNP-beta-glucopyranoside , or pNP-beta-galactopyranoside . The enzyme showed K ( M ) , V ( max ) and K ( cat ) for pNP-GlcNAc of 1 . 65mM , 79 . 49mM min ( 1 ) , and 4 . 79 x 10 ( 6 ) min ( 1 ) , respectively . The comparison of kinetic values for pNPGlcNAc and pNP-GalNAc revealed that the two enzyme activities are associated with a single binding site . The purified enzyme exhibited optimum pH and temperature for pNPGlcNAc of 5 . 0 and 50 degrees C , respectively . The enzyme activity for pNP-GlcNAc was stable at pH 5 . 0-5 . 5 and 20-40 degrees C The enzyme activity was completely inhibited at a concentration of 0 . 1 mM HgCl ( 2 ) and AgNO ( 3 ) , suggesting that the intact thiol group is essential for activity . Chloramine T completely inhibited the activity , indicating the possible involvement of methionines in the mechanism of the enzyme .
[ Sen. 9, subscore: 1.00 ]: The molecular mass was estimated to be about 52 kDa on SDS-PAGE and 37 . 4 kDa on Sephacryl S 300 gel filtration . The enzyme catalyzed the hydrolysis of both p-nitrophenyl-N-acetyl-beta-D-glucosaminide ( pNP-GlcNAc ) and p-nitrophenyl-N-acetyl-beta-D-galactosaminide ( pNPGalNAc ) as substrates , which are typical properties of beta-HexNAcase . The ratio of the pNP-GlcNAcase activity to the pNP-GalNAcase activity was 4 . 0 . However , it could not hydrolyze chitin , chitosan , pNP-beta-glucopyranoside , or pNP-beta-galactopyranoside . The enzyme showed K ( M ) , V ( max ) and K ( cat ) for pNP-GlcNAc of 1 . 65mM , 79 . 49mM min ( 1 ) , and 4 . 79 x 10 ( 6 ) min ( 1 ) , respectively . The comparison of kinetic values for pNPGlcNAc and pNP-GalNAc revealed that the two enzyme activities are associated with a single binding site . The purified enzyme exhibited optimum pH and temperature for pNPGlcNAc of 5 . 0 and 50 degrees C , respectively . The enzyme activity for pNP-GlcNAc was stable at pH 5 . 0-5 . 5 and 20-40 degrees C The enzyme activity was completely inhibited at a concentration of 0 . 1 mM HgCl ( 2 ) and AgNO ( 3 ) , suggesting that the intact thiol group is essential for activity . Chloramine T completely inhibited the activity , indicating the possible involvement of methionines in the mechanism of the enzyme .
[ Sen. 11, subscore: 1.00 ]: The ratio of the pNP-GlcNAcase activity to the pNP-GalNAcase activity was 4 . 0 . However , it could not hydrolyze chitin , chitosan , pNP-beta-glucopyranoside , or pNP-beta-galactopyranoside . The enzyme showed K ( M ) , V ( max ) and K ( cat ) for pNP-GlcNAc of 1 . 65mM , 79 . 49mM min ( 1 ) , and 4 . 79 x 10 ( 6 ) min ( 1 ) , respectively . The comparison of kinetic values for pNPGlcNAc and pNP-GalNAc revealed that the two enzyme activities are associated with a single binding site . The purified enzyme exhibited optimum pH and temperature for pNPGlcNAc of 5 . 0 and 50 degrees C , respectively . The enzyme activity for pNP-GlcNAc was stable at pH 5 . 0-5 . 5 and 20-40 degrees C The enzyme activity was completely inhibited at a concentration of 0 . 1 mM HgCl ( 2 ) and AgNO ( 3 ) , suggesting that the intact thiol group is essential for activity . Chloramine T completely inhibited the activity , indicating the possible involvement of methionines in the mechanism of the enzyme .
[ Sen. 12, subscore: 1.00 ]: However , it could not hydrolyze chitin , chitosan , pNP-beta-glucopyranoside , or pNP-beta-galactopyranoside . The enzyme showed K ( M ) , V ( max ) and K ( cat ) for pNP-GlcNAc of 1 . 65mM , 79 . 49mM min ( 1 ) , and 4 . 79 x 10 ( 6 ) min ( 1 ) , respectively . The comparison of kinetic values for pNPGlcNAc and pNP-GalNAc revealed that the two enzyme activities are associated with a single binding site . The purified enzyme exhibited optimum pH and temperature for pNPGlcNAc of 5 . 0 and 50 degrees C , respectively . The enzyme activity for pNP-GlcNAc was stable at pH 5 . 0-5 . 5 and 20-40 degrees C The enzyme activity was completely inhibited at a concentration of 0 . 1 mM HgCl ( 2 ) and AgNO ( 3 ) , suggesting that the intact thiol group is essential for activity . Chloramine T completely inhibited the activity , indicating the possible involvement of methionines in the mechanism of the enzyme .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Maporal viral infection in the Syrian golden hamster : a model of hantavirus pulmonary syndrome .
| | Author: Milazzo ML Eyzaguirre EJ Molina CP Fulhorst CF .
| | Journal: J Infect . Dis . Citation: V : 186 ( 10 ) P : 1390-5 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12404153 Accession (PMID): 12404153 | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: Hantavirus pulmonary syndrome ( HPS ) is a severe and often fatal rodent-borne zoonosis . Maporal ( MAP ) virus is a newly discovered hantavirus that originally was isolated from an arboreal rice rat captured in central Venezuela . The results of this study indicate that MAP virus in the Syrian golden hamster ( Mesocricetus auratus ) can cause a disease that is clinically and pathologically remarkably similar to HPS . The similarities include the time course of clinical disease , presence of virus-specific IgG at the onset of clinical disease , subacute pneumonitis , rapid onset of diffuse alveolar edema in the absence of necrosis , hepatic-portal triaditis , mononuclear-cellular infiltrate in lung and liver , widespread distribution of hantaviral antigen in endothelial cells of the microvasculature of lung and other it issues , and variable lethality . These similarities suggest that the MAP virus-hamster system is a useful model for studies of the pathogenesis of HPS and for the evaluation of potential therapeutic agents .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
|---|
| Title: [ Leaf photosynthetic acclimation of Echinochloa crusgalli grown in rice field to free-air CO2 enrichment ( FACE ) ] | | Author: Chen G Liao Y Cai S Zeng Q Zhu J Han Y Liu G Xu D | | Journal: Citation: V : 13 ( 10 ) P : 1201-4 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12557659 Accession (PMID): 12557659 | Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: A comparative study was made between the leaves of Echinochloa crusgalli grown at 580 ( FACE ) and 380 ( control , ambient air ) mumol CO2 mol-1 air by observation of net photosynthetic rates ( Pn ) and the contents of soluble protein and the key enzyme of photosynthetic carbon assimilation , Rubisco When measured at the ambient air CO2 concentration ( 380 mumol . mol-1 ) , Pn were significantly lower in leaves grown under FACE conditions than that in those leaves grown in the ambient air . This indicates that photosynthetic acclimation to high CO2 occurs in the leaves grown under FACE conditions . Also , the stomatal conductance ( Gs ) and intercellular CO2 concentration ( Ci ) were decreased significantly in the leaves grown under FACE . The content of total soluble protein was much lower in the leaves grown under FACE conditions than that the in the control , and the content of Rubisco was also decreased in the FACE leaves , but the difference was not significant . From these results it is deduced that the photosynthetic acclimation to high CO2 in Echinochloa crusgalli leaves grown under FACE conditions is mainly related to the partial closure of stomata and the decrease in soluble protein containing some enzymes .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
|---|
| Title: [ Response and acclimation of photosynthesis in rice leaves to free-air CO2 enrichment ( FACE ) ] | | Author: Liao Y Chen G Zhang H Cai S Zhu J Han Y Liu G Xu D | | Journal: Citation: V : 13 ( 10 ) P : 1205-9 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12557660 Accession (PMID): 12557660 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The net photosynthetic rate ( Pn ) , water use efficiency ( WUE ) , and apparent quantum yield of carbon assimilation of rice leaves were boserved contrastively under ambient air ( 380 mumol . mol-1 CO2 ) and FACE ( 580 mumol . mol-1CO2 ) . The results showed that the observed index under FACE were significantly higher than those under ambient air . Nevertheless , along with the time of high CO2 treatment prolonged , the enhancement effect of high CO2 on net photosynthetic rate declined gradually . At the same CO2 concentration , Pn and carboxylation efficiency ( CE ) in rice leaves under FACE were lower than those under ambient air . Although the stomatal conductances in FACE leaves was obviously lower than that in ambient leaves , their intercellular CO2 concentrations were not significantly different , which implied that the photosynthetic down-regulation in rice leaves grown under FACE was not caused by the decrease of stomatal conductance .
[ Sen. 4, subscore: 1.00 ]: The net photosynthetic rate ( Pn ) , water use efficiency ( WUE ) , and apparent quantum yield of carbon assimilation of rice leaves were boserved contrastively under ambient air ( 380 mumol . mol-1 CO2 ) and FACE ( 580 mumol . mol-1CO2 ) . The results showed that the observed index under FACE were significantly higher than those under ambient air . Nevertheless , along with the time of high CO2 treatment prolonged , the enhancement effect of high CO2 on net photosynthetic rate declined gradually . At the same CO2 concentration , Pn and carboxylation efficiency ( CE ) in rice leaves under FACE were lower than those under ambient air . Although the stomatal conductances in FACE leaves was obviously lower than that in ambient leaves , their intercellular CO2 concentrations were not significantly different , which implied that the photosynthetic down-regulation in rice leaves grown under FACE was not caused by the decrease of stomatal conductance .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Long-term feeding effects of heated and fried oils on hepatic antioxidant enzymes , absorption and excretion of fat in rats .
| | Author: Purushothama S Ramachandran HD Narasimhamurthy K Raina PL .
| | Journal: Mol . Cell . Biochem . Citation: V : 247 ( 1-2 ) P : 95-9 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12841636 Accession (PMID): 12841636 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Long-term feeding effect of heated and fried peanut ( PNO ) , rice bran ( RBO ) and palm oil ( PO ) in the diet on the hepatic antioxidant enzyme status and absorption and excretion of fats were studied in laboratory rats . The rats were fed oils heated to 180 degrees C continuously for a period of 72 h or laboratory fried at 20% level in the diet for 18 weeks . The results of the study indicated a significant increase in the catalase activity in HO groups and decrease in the FRO groups . The GPx activity while significantly low in HO groups was high in FRO groups , whereas , significant decrease in GST activity was observed in both PNO-HO/FRO groups . Increased activity was noted in RBO-FRO and PO-HO/FRO groups .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 7.00 |
|---|
| Title: Molecular marker dissection of rice ( Oryza sativa L ) plant architecture under temperate and tropical climates .
| | Author: Kobayashi S Fukuta Y Sato T Osaki M Khush GS .
| | Journal: Theor . Appl . Genet . Citation: V : 107 ( 8 ) P : 1350-6 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12920520 Accession (PMID): 12920520 | Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: Rice ( Oryza sativa L ) plants develop vertically with shoot elongation and horizontally with tillering . The purpose of this study was to identify and characterize genomic regions influencing the rice plant architecture by quantitative trait locus ( QTL ) analysis for the component traits : culm length ( CL ) , panicle length ( PnL ) , panicle number ( PnN ) and tiller number ( TN ) . For this QTL analysis , 191 recombinant inbred lines ( F ( 7 ) ) derived from a cross of Milyang 23 ( M23 ) and Akihikari ( AK ) were grown in 1995 , 1996 and 1997 ( May-Oct ) in Joetsu , Japan ( temperate climate ) , and in the 2000 dry season ( Jan-Apr ) , the 2000 wet season ( Jun-Oct ) and the 2001 dry season in Los Baos , The Philippines ( tropical climate ) . Results showed that rice plant architecture was influenced by 19 genomic regions categorized into five groups . In Group I , two regions ( on chrs . 6 and 11 ) affected shoot elongation ( CL and PnL ) and tillering ( PnN and TN ) in opposite directions more significantly in Los Baos than in Joetsu .
[ Sen. 6, subscore: 2.00 ]: The purpose of this study was to identify and characterize genomic regions influencing the rice plant architecture by quantitative trait locus ( QTL ) analysis for the component traits : culm length ( CL ) , panicle length ( PnL ) , panicle number ( PnN ) and tiller number ( TN ) . For this QTL analysis , 191 recombinant inbred lines ( F ( 7 ) ) derived from a cross of Milyang 23 ( M23 ) and Akihikari ( AK ) were grown in 1995 , 1996 and 1997 ( May-Oct ) in Joetsu , Japan ( temperate climate ) , and in the 2000 dry season ( Jan-Apr ) , the 2000 wet season ( Jun-Oct ) and the 2001 dry season in Los Baos , The Philippines ( tropical climate ) . Results showed that rice plant architecture was influenced by 19 genomic regions categorized into five groups . In Group I , two regions ( on chrs . 6 and 11 ) affected shoot elongation ( CL and PnL ) and tillering ( PnN and TN ) in opposite directions more significantly in Los Baos than in Joetsu . In Group II , two regions ( chrs . 3 and 12 ) affected shoot elongation , whereas in Group III , five regions [ chrs . 1 ( two ) , 2 , 3 and 9 ] affected only culm length ( CL ) . Expressions of four regions of Group III were influenced by either tropical or temperate environments .
[ Sen. 12, subscore: 2.00 ]: 3 and 12 ) affected shoot elongation , whereas in Group III , five regions [ chrs . 1 ( two ) , 2 , 3 and 9 ] affected only culm length ( CL ) . Expressions of four regions of Group III were influenced by either tropical or temperate environments . In Group IV , seven regions ( chrs . 1 , 2 , 4 , 5 , 6 , 8 and 9 ) controlled panicle development ( PnN or PnL ) , and in Group V , three regions ( chrs . 1 , 2 and 3 ) regulated tillering ( PnN or TN ) . Characterizing these 19 genomic regions provided a detailed analysis of rice plant architecture with emphasis on the multiple effect and environmental responsive regions .
[ Sen. 13, subscore: 1.00 ]: 1 ( two ) , 2 , 3 and 9 ] affected only culm length ( CL ) . Expressions of four regions of Group III were influenced by either tropical or temperate environments . In Group IV , seven regions ( chrs . 1 , 2 , 4 , 5 , 6 , 8 and 9 ) controlled panicle development ( PnN or PnL ) , and in Group V , three regions ( chrs . 1 , 2 and 3 ) regulated tillering ( PnN or TN ) . Characterizing these 19 genomic regions provided a detailed analysis of rice plant architecture with emphasis on the multiple effect and environmental responsive regions .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 8.00 |
|---|
| Title: [ Analysis of additive and AE interaction effects of QTLs controlling plant height , heading date and panicle number in rice ( Oryza sativa L ) ] | | Author: Yuan AP Cao LY Zhuang JY Li RZ Zheng KL Zhu J Cheng SH .
| | Journal: Yi Chuan Xue Bao Citation: V : 30 ( 10 ) P : 899-906 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub14669505 Accession (PMID): 14669505 | Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: Appropriate plant height , heading date and panicle number are prerequisites for the desired high and stable yield level in rice breeding programs . A recombinant inbred line ( RIL ) population consisting of 304 individuals was derived from a cross between indica varieties Zhong156 and Gumei2 , from which a linkage map consisting of 168 RFLP , SSLP , RAPD and RGA markers that distribute on all the 12 rice chromosomes was constructed , and covers 1447 . 9 cM of the rice genome . The parents and 304 F9 lines were grown in the paddy field in China National Rice Research Institute ( CNRRI ) , Hangzhou , China in 2001 . The experiments were carried out in two seasons followed a randomized complete block design . QTLMapper 1 . 01 was applied to detect QTLs and QTL x environment ( QE ) interaction for HD ( heading data ) , PH ( plant height ) and PN ( panicle number ) , and conditional mapping for PH and PN was performed as well . A total of 15 QTLs with significant additive effects were detected , among which 4 QTLs had significant QE interaction . Ten QTLs with additive x additive epistatic effects for PH , HD and PN were detected , among which none showed significant epistatisis x environment interaction . These QTLs explained 12 . 12% , 1 . 38% and 5 . 00% of the total phenotypic variance for PH , HD and PN , respectively , and contributions were generally lower due to the strong epistatic effects . In conditional QTL analysis , the numbers of QTLs showing significant additive and epistatic effects were 7 and 6 for PH , and 3 and 3 for PN , respectively .
[ Sen. 1, subscore: 1.00 ]: Plant height ( PH ) , heading date ( HD ) and productive panicle number ( PN ) are important agronomic trait in rice . Appropriate plant height , heading date and panicle number are prerequisites for the desired high and stable yield level in rice breeding programs . A recombinant inbred line ( RIL ) population consisting of 304 individuals was derived from a cross between indica varieties Zhong156 and Gumei2 , from which a linkage map consisting of 168 RFLP , SSLP , RAPD and RGA markers that distribute on all the 12 rice chromosomes was constructed , and covers 1447 . 9 cM of the rice genome . The parents and 304 F9 lines were grown in the paddy field in China National Rice Research Institute ( CNRRI ) , Hangzhou , China in 2001 . The experiments were carried out in two seasons followed a randomized complete block design .
[ Sen. 8, subscore: 1.00 ]: The parents and 304 F9 lines were grown in the paddy field in China National Rice Research Institute ( CNRRI ) , Hangzhou , China in 2001 . The experiments were carried out in two seasons followed a randomized complete block design . QTLMapper 1 . 01 was applied to detect QTLs and QTL x environment ( QE ) interaction for HD ( heading data ) , PH ( plant height ) and PN ( panicle number ) , and conditional mapping for PH and PN was performed as well . A total of 15 QTLs with significant additive effects were detected , among which 4 QTLs had significant QE interaction . Ten QTLs with additive x additive epistatic effects for PH , HD and PN were detected , among which none showed significant epistatisis x environment interaction . These QTLs explained 12 . 12% , 1 . 38% and 5 . 00% of the total phenotypic variance for PH , HD and PN , respectively , and contributions were generally lower due to the strong epistatic effects . In conditional QTL analysis , the numbers of QTLs showing significant additive and epistatic effects were 7 and 6 for PH , and 3 and 3 for PN , respectively . Among the QTLs having significant additive effects for PH , qPH7-2 showed both additive effects and QE interaction , qPH7-1 and qPH10 showed QE interaction only , and the remaining 4 QTLs showed additive effects only . Each of the 3 QTLs having significant additive effects for PN did not display significant QE interaction .
[ Sen. 9, subscore: 1.00 ]: The experiments were carried out in two seasons followed a randomized complete block design . QTLMapper 1 . 01 was applied to detect QTLs and QTL x environment ( QE ) interaction for HD ( heading data ) , PH ( plant height ) and PN ( panicle number ) , and conditional mapping for PH and PN was performed as well . A total of 15 QTLs with significant additive effects were detected , among which 4 QTLs had significant QE interaction . Ten QTLs with additive x additive epistatic effects for PH , HD and PN were detected , among which none showed significant epistatisis x environment interaction . These QTLs explained 12 . 12% , 1 . 38% and 5 . 00% of the total phenotypic variance for PH , HD and PN , respectively , and contributions were generally lower due to the strong epistatic effects . In conditional QTL analysis , the numbers of QTLs showing significant additive and epistatic effects were 7 and 6 for PH , and 3 and 3 for PN , respectively . Among the QTLs having significant additive effects for PH , qPH7-2 showed both additive effects and QE interaction , qPH7-1 and qPH10 showed QE interaction only , and the remaining 4 QTLs showed additive effects only . Each of the 3 QTLs having significant additive effects for PN did not display significant QE interaction . No epistatic QE interactions was detected .
[ Sen. 10, subscore: 1.00 ]: QTLMapper 1 . 01 was applied to detect QTLs and QTL x environment ( QE ) interaction for HD ( heading data ) , PH ( plant height ) and PN ( panicle number ) , and conditional mapping for PH and PN was performed as well . A total of 15 QTLs with significant additive effects were detected , among which 4 QTLs had significant QE interaction . Ten QTLs with additive x additive epistatic effects for PH , HD and PN were detected , among which none showed significant epistatisis x environment interaction . These QTLs explained 12 . 12% , 1 . 38% and 5 . 00% of the total phenotypic variance for PH , HD and PN , respectively , and contributions were generally lower due to the strong epistatic effects . In conditional QTL analysis , the numbers of QTLs showing significant additive and epistatic effects were 7 and 6 for PH , and 3 and 3 for PN , respectively . Among the QTLs having significant additive effects for PH , qPH7-2 showed both additive effects and QE interaction , qPH7-1 and qPH10 showed QE interaction only , and the remaining 4 QTLs showed additive effects only . Each of the 3 QTLs having significant additive effects for PN did not display significant QE interaction . No epistatic QE interactions was detected . In addition , conditional QTL analysis indicated that the expression of QTLs for PH and PN may vary depending on the QTLs for HD .
[ Sen. 12, subscore: 1.00 ]: Ten QTLs with additive x additive epistatic effects for PH , HD and PN were detected , among which none showed significant epistatisis x environment interaction . These QTLs explained 12 . 12% , 1 . 38% and 5 . 00% of the total phenotypic variance for PH , HD and PN , respectively , and contributions were generally lower due to the strong epistatic effects . In conditional QTL analysis , the numbers of QTLs showing significant additive and epistatic effects were 7 and 6 for PH , and 3 and 3 for PN , respectively . Among the QTLs having significant additive effects for PH , qPH7-2 showed both additive effects and QE interaction , qPH7-1 and qPH10 showed QE interaction only , and the remaining 4 QTLs showed additive effects only . Each of the 3 QTLs having significant additive effects for PN did not display significant QE interaction . No epistatic QE interactions was detected . In addition , conditional QTL analysis indicated that the expression of QTLs for PH and PN may vary depending on the QTLs for HD .
[ Sen. 14, subscore: 1.00 ]: In conditional QTL analysis , the numbers of QTLs showing significant additive and epistatic effects were 7 and 6 for PH , and 3 and 3 for PN , respectively . Among the QTLs having significant additive effects for PH , qPH7-2 showed both additive effects and QE interaction , qPH7-1 and qPH10 showed QE interaction only , and the remaining 4 QTLs showed additive effects only . Each of the 3 QTLs having significant additive effects for PN did not display significant QE interaction . No epistatic QE interactions was detected . In addition , conditional QTL analysis indicated that the expression of QTLs for PH and PN may vary depending on the QTLs for HD .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Expressional analysis of an EREBP transcription factor gene OsEBP-89 in rice .
| | Author: Shen H Wang ZY .
| | Journal: Acta Biochim . Biophys . Sin . ( Shanghai ) Citation: V : 36 ( 1 ) P : 21-6 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub14732870 Accession (PMID): 14732870 | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: OsEBP-89 gene encodes an ethylene responsive element binding protein ( EREBP ) transcription factor from rice ( Oryza sativa ) . Northern blot analysis revealed that OsEBP-89 was expressed in root , stem , seeds , flowers and leaves of rice . Histochemical assay showed that GUS expressed mainly in phloem of vascular it issues of the root and stem transition region ( RST ) , basal part of sheath roots , stem node and basal part of adventitious roots , also in endosperm of seeds in transgenic rice harboring OsEBP-89/GUS construct ( pNSG ) . A sequence of region from C279 to C97 was found to play an important role for OsEBP-89 genes expression though promoter deletion assay . The possible function of OsEBP-89 gene was discussed .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Klebsiella variicola , a novel species with clinical and plant-associated isolates .
| | Author: Rosenblueth M Martez L Silva J Martez-Romero E | | Journal: Syst Appl . Microbiol . Citation: V : 27 ( 1 ) P : 27-35 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15053318 Accession (PMID): 15053318 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: A new Klebsiella species , K variicola , is proposed on the basis of total DNA-DNA hybridization , on the monophyly observed in the phylogenetic analysis derived from the sequences of rpoB , gyrA , mdh , infB , phoE and nifH genes and on distinct phenotypic traits . The bacteria from this new species seem to be genetically isolated from K pneumoniae strains , do not ferment adonitol and were obtained from plants ( such as banana , rice , sugar cane and maize ) and hospitals . The type strain is F2R9T ( = ATCC BAA-830T = CFNE 2004T ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
|---|
| Title: Rice dwarf phytoreovirus segment S6-encoded nonstructural protein has a cell-to-cell movement function .
| | Author: Li Y Bao YM Wei CH Kang ZS Zhong YW Mao P Wu G Chen ZL Schiemann J Nelson RS .
| | Journal: J Virol .
Citation: V : 78 ( 10 ) P : 5382-9 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15113917 Accession (PMID): 15113917 | Matching Sentences:
[ Sen. 10, subscore: 1.00 ]: In the absence of S6 , no other gene segment supported movement . Identical results were obtained with Nicotiana tabacum , a host that allows fewer viruses to infect and spread within its it issue . S6 supported the cell-to-cell movement of the movement-defective PVX in sink and source leaves of N benthamiana . A mutant S6 lacking the translation start codon did not complement the cell-to-cell movement of the movement-defective PVX . An S6 protein product ( Pns6 ) -enhanced GFP fusion was observed near or within cell walls of epidermal cells from N tabacum . By immunocytochemistry , unfused Pns6 was localized to plasmodesmata in rice leaves infected with RDV . S6 thus encodes a protein with characteristics identical to those of other viral proteins required for the cell-to-cell movement of their genome and therefore is likely required for the cell-to-cell movement of RDV .
[ Sen. 11, subscore: 1.00 ]: Identical results were obtained with Nicotiana tabacum , a host that allows fewer viruses to infect and spread within its it issue . S6 supported the cell-to-cell movement of the movement-defective PVX in sink and source leaves of N benthamiana . A mutant S6 lacking the translation start codon did not complement the cell-to-cell movement of the movement-defective PVX . An S6 protein product ( Pns6 ) -enhanced GFP fusion was observed near or within cell walls of epidermal cells from N tabacum . By immunocytochemistry , unfused Pns6 was localized to plasmodesmata in rice leaves infected with RDV . S6 thus encodes a protein with characteristics identical to those of other viral proteins required for the cell-to-cell movement of their genome and therefore is likely required for the cell-to-cell movement of RDV .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
|---|
| Title: Quantitative trait loci associated with drought tolerance at reproductive stage in rice .
| | Author: Lanceras JC Pantuwan G Jongdee B Toojinda T | | Journal: Plant Physiol .
Citation: V : 135 ( 1 ) P : 384-99 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15122029 Accession (PMID): 15122029 | Matching Sentences:
[ Sen. 7, subscore: 1.00 ]: Quantitative trait loci ( QTL ) for grain yield and its components and other agronomic traits were identified using a subset of 154 doubled haploid lines derived from a cross between two rice cultivars , CT9993-510 to 1-M and IR62266-42 to 6-2 . Drought stress treatments were managed by use of a line source sprinkler irrigation system , which provided a linearly decreasing level of irrigation coinciding with the sensitive reproductive growth stages . The research was conducted at the Ubon Rice Research Center , Ubon , Thailand . A total of 77 QTL were identified for grain yield and its components under varying levels of water stress . Out of the total of 77 QTL , the number of QTL per trait were : 7-grain yield ( GY ) ; 8-biological yield ( BY ) ; 6-harvest index ( HI ) ; 5-d to flowering after initiation of irrigation gradient ( DFAIG ) ; 10-total spikelet number ( TSN ) ; 7-percent spikelet sterility ( PSS ) ; 23-panicle number ( PN ) ; and 11-plant height ( PH ) . The phenotypic variation explained by individual QTL ranged from 7 . 5% to 55 . 7% . Under well-watered conditions , we observed a high genetic association for BY , HI , DFAIG , PSS , TSN , PH , and GY . However , only BY and HI were found to be significantly associated with GY under drought treatments . QTL flanked by markers RG104 to RM231 , EMP2_2 to RM127 , and G2132 to RZ598 on chromosomes 3 , 4 , and 8 were associated with GY , HI , DFAIG , BY , PSS , and PN under drought treatments .
[ Sen. 11, subscore: 1.00 ]: Out of the total of 77 QTL , the number of QTL per trait were : 7-grain yield ( GY ) ; 8-biological yield ( BY ) ; 6-harvest index ( HI ) ; 5-d to flowering after initiation of irrigation gradient ( DFAIG ) ; 10-total spikelet number ( TSN ) ; 7-percent spikelet sterility ( PSS ) ; 23-panicle number ( PN ) ; and 11-plant height ( PH ) . The phenotypic variation explained by individual QTL ranged from 7 . 5% to 55 . 7% . Under well-watered conditions , we observed a high genetic association for BY , HI , DFAIG , PSS , TSN , PH , and GY . However , only BY and HI were found to be significantly associated with GY under drought treatments . QTL flanked by markers RG104 to RM231 , EMP2_2 to RM127 , and G2132 to RZ598 on chromosomes 3 , 4 , and 8 were associated with GY , HI , DFAIG , BY , PSS , and PN under drought treatments . The aggregate effects of these QTL on chromosomes 3 , 4 , and 8 resulted in higher grain yield . These QTL will be useful for rainfed rice improvement , and will also contribute to our understanding of the genetic control of GY under drought conditions at the sensitive reproductive stage . Close linkage or pleiotropy may be responsible for the coincidence of QTL detected in this experiment . Digenic interactions between QTL main effects for GY , BY , HI , and PSS were observed under irrigation treatments .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Recombinant human lactoferrin treatment for global health issues : iron deficiency and acute diarrhea .
| | Author: Bethell DR Huang J | | Journal: Biometals Citation: V : 17 ( 3 ) P : 337-42 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15222487 Accession (PMID): 15222487 | Matching Sentences:
[ Sen. 8, subscore: 1.00 ]: The prevalence is highest among young children and women of childbearing age ( particularly pregnant women ) . It is estimated that national productivity levels could be raised as much as 20% by correcting iron deficiency in developing countries . Recombinant human lactoferrin ( rhLF ) , expressed and extracted from rice seed , is being evaluated by Ventria Bioscience for use as a dietary supplement to treat iron deficiency and/or iron deficiency anemia . Diarrhea is also a major world health issue . Sixty percent of children who die under age five die of pneumonia , diarrhea or measles . World Health Organization oral rehydration solution ( WHO-ORS ) is one of the major medical advances in the past 50 years , saving the lives of 1 to 2 million children annually . Many studies have demonstrated similar efficacy of rice-based ORS . There are studies documenting the reduced frequency of diarrhea in breast-fed children and this health improvement is attributed to the antimicrobial action of the human milk proteins lactoferrin and lysozyme . In vitro data document the growth inhibition of the diarrheal associated organisms : rotavirus , ETEC , cholera , salmonella , and shigella by human lactoferrin ( hLF ) and human lysozyme .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 8.00 |
|---|
| Title: Nucleic acid binding activity of pns6 encoded by genome segment 6 of rice ragged stunt oryzavirus .
| | Author: Shao CG L HJ Wu JH Gong ZX .
| | Journal: Acta Biochim . Biophys . Sin . ( Shanghai ) Citation: V : 36 ( 7 ) P : 457-66 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15248020 Accession (PMID): 15248020 | Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: Pns6 , the 71 kD product of S6 expressed in E coli , was demonstrated to be a viral non-structural protein of RRSV by Western blotting . The gel mobility shift assays showed that Pns6 had nucleic acid binding activity . Pns6 could interact with single and double-stranded forms of DNA and RNA , showing a preference for single-stranded nucleic acid and a slight preference for RRSV ssRNA over the rice ssRNA , as demonstrated by both competition and displacement assays . The binding of Pns6 to nucleic acids is strong and sequence non-specific . By using five truncated derivatives of Pns6 , it was found that the basic region from amino acid 201 to 273 of Pns6 was the unique nucleic acid binding domain . Subcellular fractionation of leaf it issues of RRSV-infected rice plants and subsequent Western blotting had shown that Pns6 accumulated predominately in the cytoplasmic membrane fraction . The possible role of RRSV Pns6 in virus replication and assembly is discussed .
[ Sen. 2, subscore: 1.00 ]: The ORF of genome segment 6 ( S6 ) of rice ragged stunt oryzavirus ( RRSV ) Philippines isolate was cloned and sequenced based on the S6 sequence of the Thailand isolate . Pns6 , the 71 kD product of S6 expressed in E coli , was demonstrated to be a viral non-structural protein of RRSV by Western blotting . The gel mobility shift assays showed that Pns6 had nucleic acid binding activity . Pns6 could interact with single and double-stranded forms of DNA and RNA , showing a preference for single-stranded nucleic acid and a slight preference for RRSV ssRNA over the rice ssRNA , as demonstrated by both competition and displacement assays . The binding of Pns6 to nucleic acids is strong and sequence non-specific . By using five truncated derivatives of Pns6 , it was found that the basic region from amino acid 201 to 273 of Pns6 was the unique nucleic acid binding domain .
[ Sen. 3, subscore: 1.00 ]: The ORF of genome segment 6 ( S6 ) of rice ragged stunt oryzavirus ( RRSV ) Philippines isolate was cloned and sequenced based on the S6 sequence of the Thailand isolate . Pns6 , the 71 kD product of S6 expressed in E coli , was demonstrated to be a viral non-structural protein of RRSV by Western blotting . The gel mobility shift assays showed that Pns6 had nucleic acid binding activity . Pns6 could interact with single and double-stranded forms of DNA and RNA , showing a preference for single-stranded nucleic acid and a slight preference for RRSV ssRNA over the rice ssRNA , as demonstrated by both competition and displacement assays . The binding of Pns6 to nucleic acids is strong and sequence non-specific . By using five truncated derivatives of Pns6 , it was found that the basic region from amino acid 201 to 273 of Pns6 was the unique nucleic acid binding domain . Subcellular fractionation of leaf it issues of RRSV-infected rice plants and subsequent Western blotting had shown that Pns6 accumulated predominately in the cytoplasmic membrane fraction .
[ Sen. 4, subscore: 1.00 ]: The ORF of genome segment 6 ( S6 ) of rice ragged stunt oryzavirus ( RRSV ) Philippines isolate was cloned and sequenced based on the S6 sequence of the Thailand isolate . Pns6 , the 71 kD product of S6 expressed in E coli , was demonstrated to be a viral non-structural protein of RRSV by Western blotting . The gel mobility shift assays showed that Pns6 had nucleic acid binding activity . Pns6 could interact with single and double-stranded forms of DNA and RNA , showing a preference for single-stranded nucleic acid and a slight preference for RRSV ssRNA over the rice ssRNA , as demonstrated by both competition and displacement assays . The binding of Pns6 to nucleic acids is strong and sequence non-specific . By using five truncated derivatives of Pns6 , it was found that the basic region from amino acid 201 to 273 of Pns6 was the unique nucleic acid binding domain . Subcellular fractionation of leaf it issues of RRSV-infected rice plants and subsequent Western blotting had shown that Pns6 accumulated predominately in the cytoplasmic membrane fraction . The possible role of RRSV Pns6 in virus replication and assembly is discussed .
[ Sen. 5, subscore: 1.00 ]: The ORF of genome segment 6 ( S6 ) of rice ragged stunt oryzavirus ( RRSV ) Philippines isolate was cloned and sequenced based on the S6 sequence of the Thailand isolate . Pns6 , the 71 kD product of S6 expressed in E coli , was demonstrated to be a viral non-structural protein of RRSV by Western blotting . The gel mobility shift assays showed that Pns6 had nucleic acid binding activity . Pns6 could interact with single and double-stranded forms of DNA and RNA , showing a preference for single-stranded nucleic acid and a slight preference for RRSV ssRNA over the rice ssRNA , as demonstrated by both competition and displacement assays . The binding of Pns6 to nucleic acids is strong and sequence non-specific . By using five truncated derivatives of Pns6 , it was found that the basic region from amino acid 201 to 273 of Pns6 was the unique nucleic acid binding domain . Subcellular fractionation of leaf it issues of RRSV-infected rice plants and subsequent Western blotting had shown that Pns6 accumulated predominately in the cytoplasmic membrane fraction . The possible role of RRSV Pns6 in virus replication and assembly is discussed .
[ Sen. 7, subscore: 1.00 ]: The gel mobility shift assays showed that Pns6 had nucleic acid binding activity . Pns6 could interact with single and double-stranded forms of DNA and RNA , showing a preference for single-stranded nucleic acid and a slight preference for RRSV ssRNA over the rice ssRNA , as demonstrated by both competition and displacement assays . The binding of Pns6 to nucleic acids is strong and sequence non-specific . By using five truncated derivatives of Pns6 , it was found that the basic region from amino acid 201 to 273 of Pns6 was the unique nucleic acid binding domain . Subcellular fractionation of leaf it issues of RRSV-infected rice plants and subsequent Western blotting had shown that Pns6 accumulated predominately in the cytoplasmic membrane fraction . The possible role of RRSV Pns6 in virus replication and assembly is discussed .
[ Sen. 8, subscore: 1.00 ]: Pns6 could interact with single and double-stranded forms of DNA and RNA , showing a preference for single-stranded nucleic acid and a slight preference for RRSV ssRNA over the rice ssRNA , as demonstrated by both competition and displacement assays . The binding of Pns6 to nucleic acids is strong and sequence non-specific . By using five truncated derivatives of Pns6 , it was found that the basic region from amino acid 201 to 273 of Pns6 was the unique nucleic acid binding domain . Subcellular fractionation of leaf it issues of RRSV-infected rice plants and subsequent Western blotting had shown that Pns6 accumulated predominately in the cytoplasmic membrane fraction . The possible role of RRSV Pns6 in virus replication and assembly is discussed .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 4.00 |
|---|
| Title: The effect of individualized diet challenges consisting of allergenic foods on TNF-alpha and IL-1beta levels in patients with rheumatoid arthritis .
| | Author: Karatay S Erdem T Yildirim K Melikoglu MA Ugur M Cakir E Akcay F Senel K | | Journal: Citation: V : 43 ( 11 ) P : 1429-33 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15304675 Accession (PMID): 15304675 | Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: OBJECTIVE : To investigate the effect of individualized diet challenges consisting of allergenic foods , defined by the skin prick test ( SPT ) , on tumour necrosis factor-alpha ( TNF-alpha ) and interleukin-1beta ( IL-1beta ) levels in patients with rheumatoid arthritis ( RA ) . METHODS : Twenty patients with a positive SPT response for food extracts and 20 with a negative SPT response were enrolled . None of the patients had active disease . All patients were fasted for the most common allergenic foods for 12 days and then allocated to two groups according to SPT results . Food challenges were performed with allergenic foods in the prick-positive group ( PPG ) and with corn and rice in the prick-negative group ( PNG ) for a period of 12 days . Then , allergenic foods were excluded from the PPG patients diet and corn and rice were removed from the PNG patients diet . Clinical examinations were performed after fasting ( baseline ) , at the end of the challenge phase and at the end of the re-elimination phase . Stiffness , pain , tender and swollen joint counts , health assessment questionnaire ( HAQ ) , Ritchies articular index , erythrocyte sedimentation rate ( ESR ) , C-reactive protein ( CRP ) and serum TNF-alpha and IL-1beta levels were measured . RESULTS : TNF-alpha ( P < 0 . 01 ) , IL-1beta ( P < 0 . 05 ) , ESR ( P < 0 . 05 ) and CRP ( P = 0 . 001 ) levels and all of the clinical variables , except HAQ , were increased with food challenges in the PPG .
[ Sen. 6, subscore: 1.00 ]: METHODS : Twenty patients with a positive SPT response for food extracts and 20 with a negative SPT response were enrolled . None of the patients had active disease . All patients were fasted for the most common allergenic foods for 12 days and then allocated to two groups according to SPT results . Food challenges were performed with allergenic foods in the prick-positive group ( PPG ) and with corn and rice in the prick-negative group ( PNG ) for a period of 12 days . Then , allergenic foods were excluded from the PPG patients diet and corn and rice were removed from the PNG patients diet . Clinical examinations were performed after fasting ( baseline ) , at the end of the challenge phase and at the end of the re-elimination phase . Stiffness , pain , tender and swollen joint counts , health assessment questionnaire ( HAQ ) , Ritchies articular index , erythrocyte sedimentation rate ( ESR ) , C-reactive protein ( CRP ) and serum TNF-alpha and IL-1beta levels were measured . RESULTS : TNF-alpha ( P < 0 . 01 ) , IL-1beta ( P < 0 . 05 ) , ESR ( P < 0 . 05 ) and CRP ( P = 0 . 001 ) levels and all of the clinical variables , except HAQ , were increased with food challenges in the PPG . These increases were also recorded after the re-elimination phase .
[ Sen. 11, subscore: 1.00 ]: Clinical examinations were performed after fasting ( baseline ) , at the end of the challenge phase and at the end of the re-elimination phase . Stiffness , pain , tender and swollen joint counts , health assessment questionnaire ( HAQ ) , Ritchies articular index , erythrocyte sedimentation rate ( ESR ) , C-reactive protein ( CRP ) and serum TNF-alpha and IL-1beta levels were measured . RESULTS : TNF-alpha ( P < 0 . 01 ) , IL-1beta ( P < 0 . 05 ) , ESR ( P < 0 . 05 ) and CRP ( P = 0 . 001 ) levels and all of the clinical variables , except HAQ , were increased with food challenges in the PPG . These increases were also recorded after the re-elimination phase . In the PNG , no significant change was seen in any of the variables , except pain ( P < 0 . 05 ) . During the study , important differences were observed for most of the variables between the two groups . Thirteen ( 72% ) patients in the PPG and three ( 18% ) in the PNG experienced disease exacerbation with challenges . This aggravation continued after elimination . CONCLUSIONS : Our results suggest that individualized dietary revisions may regulate TNF-alpha and IL-1beta levels in selected patients with RA .
[ Sen. 13, subscore: 1.00 ]: RESULTS : TNF-alpha ( P < 0 . 01 ) , IL-1beta ( P < 0 . 05 ) , ESR ( P < 0 . 05 ) and CRP ( P = 0 . 001 ) levels and all of the clinical variables , except HAQ , were increased with food challenges in the PPG . These increases were also recorded after the re-elimination phase . In the PNG , no significant change was seen in any of the variables , except pain ( P < 0 . 05 ) . During the study , important differences were observed for most of the variables between the two groups . Thirteen ( 72% ) patients in the PPG and three ( 18% ) in the PNG experienced disease exacerbation with challenges . This aggravation continued after elimination . CONCLUSIONS : Our results suggest that individualized dietary revisions may regulate TNF-alpha and IL-1beta levels in selected patients with RA .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Similar cholesterol-lowering properties of rice bran oil , with varied gamma-oryzanol , in mildly hypercholesterolemic men* .
| | Author: Berger A Rein D Schfer A Monnard I Gremaud G Lambelet P Bertoli C | | Journal: Citation: V : 44 ( 3 ) P : 163-73 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15309429 Accession (PMID): 15309429 | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: BACKGROUND : The cholesterol lowering properties of rice bran oil ( RBO ) containing differing amounts of non-saponifiable components have not been studied in humans , to our knowledge . AIM OF THE STUDY : To evaluate cholesterol lowering effects of RBO , with low and high amounts of gamma-oryzanol ( ferulated plant sterols ) in mildly hypercholesterolemic men . METHODS : Mildly hypercholesterolemic men , 38-64 y , starting cholesterol 4 . 9-8 . 4 mmol/l ( n = 30 ) , consumed 50 g/d peanut oil ( PNO ) in vehicles for 2 wks during a run-in period , then , without wash-out , were randomly equilibrated ( based on initial level of cholesterol ) into two groups to consume 50 g/d RBO low ( 0 . 05 g/d ) or high ( 0 . 8 g/d ) gamma-oryzanol for 4 wks , in a randomized , controlled , parallel design study . Subjects were free-living and consumed habitual diets with some restrictions . Plasma concentrations of total , LDL- , HDL-cholesterol and triacylglycerol were measured at base line and after 2 , 4 , and 6 wks . RESULTS : The two RBO types were not significantly different with respect to effects on various cholesterol parameters , at 2 and 4 wks , including total cholesterol , LDL- , HDL and LDL/HDL cholesterol ratio . Low and high gamma-oryzanolcontaining RBO feeding for 4 wks lowered total plasma cholesterol ( 6 . 3 % ) , LDL-C ( 10 . 5 % ) and the LDL-C/HDL-C ratio ( 18 . 9 % ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
|---|
| Title: Identification and characterization of a QTL on chromosome 2 for cytosolic glutamine synthetase content and panicle number in rice .
| | Author: Obara M Sato T Sasaki S Kashiba K Nagano A Nakamura I Ebitani T Yano M Yamaya T | | Journal: Theor . Appl . Genet . Citation: V : 110 ( 1 ) P : 1-11 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15549232 Accession (PMID): 15549232 | Matching Sentences:
[ Sen. 9, subscore: 1.00 ]: At an early vegetative stage , C-22 had more active tillers than Koshihikari in the greenhouse . At the reproductive stage , both panicle number and total panicle weight of C-22 were significantly higher than those of Koshihikari , particularly when the plants were grown under a low-nitrogen condition . These traits of C-22 were further confirmed in a paddy field . Thus , tiller development was positively affected by the Kasalath chromosome at an early vegetative stage , which resulted in an increased panicle number and panicle weight at the mature stage in C-22 . These data indicate that the target QTL ( Pnn1 ; panicle number 1 ) is important in the development of tillers and panicles in rice . Linkage analyses for panicle number and ratio of developing tiller formation in the second axil ( RDT ) revealed that Pnn1 was delimited at the 6 . 7-cM region .
[ Sen. 10, subscore: 1.00 ]: At the reproductive stage , both panicle number and total panicle weight of C-22 were significantly higher than those of Koshihikari , particularly when the plants were grown under a low-nitrogen condition . These traits of C-22 were further confirmed in a paddy field . Thus , tiller development was positively affected by the Kasalath chromosome at an early vegetative stage , which resulted in an increased panicle number and panicle weight at the mature stage in C-22 . These data indicate that the target QTL ( Pnn1 ; panicle number 1 ) is important in the development of tillers and panicles in rice . Linkage analyses for panicle number and ratio of developing tiller formation in the second axil ( RDT ) revealed that Pnn1 was delimited at the 6 . 7-cM region .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
|---|
| Title: TM2 , a novel strong matrix attachment region isolated from tobacco , increases transgene expression in transgenic rice calli and plants .
| | Author: Xue H Yang YT Wu CA Yang GD Zhang MM Zheng CC .
| | Journal: Theor . Appl . Genet . Citation: V : 110 ( 4 ) P : 620-7 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15660239 Accession (PMID): 15660239 | Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: Nuclear matrix attachment regions ( MARs ) are thought to influence the expression of the flanking genes . TM2 , a new DNA fragment isolated from tobacco , can bind with the rice nuclear matrix in vitro . In this study , we investigated the effect of TM2 on transgene expression under the control of three different promoters in stably transformed rice calli and plants . The presence of TM2 flanking the transgene increased the expression of constructs based on the constitutive CaMV 35S and maize ubiquitin gene promoters in both resistant calli and transformed plants . The GUS expression directed by the photosynthetic-tissue-specific PNZIP promoter was also increased in photosynthetic it issues of transformants . However , TM2 did not change the gene expression pattern controlled by the PNZIP promoter . The effect of TM2 in transgenic plants was stronger than that in transgenic calli based on all three promoters . Our results indicate that TM2 , as a novel strong MAR , can be used to increase the transgene expression levels in the whole plant or in particular it issues of monocotyledons .
[ Sen. 6, subscore: 1.00 ]: TM2 , a new DNA fragment isolated from tobacco , can bind with the rice nuclear matrix in vitro . In this study , we investigated the effect of TM2 on transgene expression under the control of three different promoters in stably transformed rice calli and plants . The presence of TM2 flanking the transgene increased the expression of constructs based on the constitutive CaMV 35S and maize ubiquitin gene promoters in both resistant calli and transformed plants . The GUS expression directed by the photosynthetic-tissue-specific PNZIP promoter was also increased in photosynthetic it issues of transformants . However , TM2 did not change the gene expression pattern controlled by the PNZIP promoter . The effect of TM2 in transgenic plants was stronger than that in transgenic calli based on all three promoters . Our results indicate that TM2 , as a novel strong MAR , can be used to increase the transgene expression levels in the whole plant or in particular it issues of monocotyledons .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Occurrence of Candida strains in cases of paronychia .
| | Author: Dorko E JautovEJ Pilipcinec E TkcikovEL .
| | Journal: Folia Microbiol .
( Praha ) Citation: V : 49 ( 5 ) P : 591-5 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15702551 Accession (PMID): 15702551 | Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: A total of 43 patients , 11 males and 32 females , with paronychia of the fingernails were examined for the presence of Candida spp . The yeast species isolated were identified using standard laboratory methods , including germ-tube production , morphology on rice agar with Tween 80 , and mainly fermentation and assimilation of saccharides . In the male group , two Candida species were detected : C albicans as the dominant species in 9 patients and C parapsilosis in 2 cases . Similarly , C albicans was the prevalent species also in females ( n = 17 ) ; other Candida species detected were C parapsilosis ( n = 7 ) , C tropicalis ( 5 ) and C krusei ( 3 ) . In addition to the genus Candida , the following anaerobic and aerobic microorganisms were isolated from patients of both groups : Fusobacterium spp . , Bacteroides spp . , Staphylococcus aureus , alpha-hemolytic streptococci , group A beta-hemolytic streptococci , Klebsiella pneumoniae , Neisseria spp . and Pseudomonas aeruginosa .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: The promoter of Milk vetch dwarf virus component 8 confers effective gene expression in both dicot and monocot plants .
| | Author: Shirasawa-Seo N Sano Y Nakamura S Murakami T Gotoh Y Naito Y Hsia CN Seo S Mitsuhara I Kosugi S Ohashi Y | | Journal: Plant Cell Rep .
Citation: V : 24 ( 3 ) P : 155-63 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15812660 Accession (PMID): 15812660 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The activity of a predicted promoter , PMC8 , from Milk vetch dwarf virus was evaluated by comparing it with the cauliflower mosaic virus 35S RNA promoter ( P35S ) and PNCR , a promoter from Soybean chlorotic mottle virus . When the GUS fusion gene was introduced into tobacco , PMC8 showed a similar expression profile to P35S but with a more intense expression in proliferating it issues . The usefulness of PMC8 was confirmed by driving NPTII for selection of kanamycin-resistant tobacco plants with improved transformation efficiency . PMC8 was also effective in transgenic rice plants . Thus , PMC8 is useful as an alternative to P35S in both dicotyledonous and monocotyledonous plants , especially for gene expression in proliferating it issues .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 5.00 |
|---|
| Title: Photosynthetic acclimation in rice leaves to free-air CO2 enrichment related to both ribulose-1 , 5-bisphosphate carboxylation limitation and ribulose-1 , 5-bisphosphate regeneration limitation .
| | Author: Chen GY Yong ZH Liao Y Zhang DY Chen Y Zhang HB Chen J Zhu JG Xu DQ .
| | Journal: Plant Cell Physiol .
Citation: V : 46 ( 7 ) P : 1036-45 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15840641 Accession (PMID): 15840641 | Matching Sentences:
[ Sen. 4, subscore: 3.00 ]: Net photosynthetic rates ( Pns ) in leaves were compared between rice plants grown in ambient air control and free-air CO2 enrichment ( FACE , about 200 micromol mol ( -1 ) above ambient ) treatment rings . When measured at the same CO2 concentration , the Pn of FACE leaves decreased significantly , indicating that photosynthetic acclimation to high CO2 occurs . Although stomatal conductance ( Gs ) in FACE leaves was markedly decreased , intercellular CO2 concentrations ( Ci ) were almost the same in FACE and ambient leaves , indicating that the photosynthetic acclimation is not caused by the decreased Gs . Furthermore , carboxylation efficiency and maximal Pn , both light and CO2-saturated Pn , were decreased in FACE leaves , as shown by the Pn-Ci curves . In addition , the soluble protein , Rubisco ( ribulose-1 , 5-bisphosphate caboxylase/oxygenase ) , and its activase contents as well as the sucrose-phosphate synthase activity decreased significantly , while some soluble sugar , inorganic phosphate , chlorophyll and light-harvesting complex II ( LHC II ) contents increased in FACE leaves . It appears that the photosynthetic acclimation in rice leaves is related to both ribulose-1 , 5-bisphosphate ( RuBP ) carboxylation limitation and RuBP regeneration limitation .
[ Sen. 1, subscore: 1.00 ]: Net photosynthetic rates ( Pns ) in leaves were compared between rice plants grown in ambient air control and free-air CO2 enrichment ( FACE , about 200 micromol mol ( -1 ) above ambient ) treatment rings . When measured at the same CO2 concentration , the Pn of FACE leaves decreased significantly , indicating that photosynthetic acclimation to high CO2 occurs . Although stomatal conductance ( Gs ) in FACE leaves was markedly decreased , intercellular CO2 concentrations ( Ci ) were almost the same in FACE and ambient leaves , indicating that the photosynthetic acclimation is not caused by the decreased Gs . Furthermore , carboxylation efficiency and maximal Pn , both light and CO2-saturated Pn , were decreased in FACE leaves , as shown by the Pn-Ci curves . In addition , the soluble protein , Rubisco ( ribulose-1 , 5-bisphosphate caboxylase/oxygenase ) , and its activase contents as well as the sucrose-phosphate synthase activity decreased significantly , while some soluble sugar , inorganic phosphate , chlorophyll and light-harvesting complex II ( LHC II ) contents increased in FACE leaves .
[ Sen. 2, subscore: 1.00 ]: Net photosynthetic rates ( Pns ) in leaves were compared between rice plants grown in ambient air control and free-air CO2 enrichment ( FACE , about 200 micromol mol ( -1 ) above ambient ) treatment rings . When measured at the same CO2 concentration , the Pn of FACE leaves decreased significantly , indicating that photosynthetic acclimation to high CO2 occurs . Although stomatal conductance ( Gs ) in FACE leaves was markedly decreased , intercellular CO2 concentrations ( Ci ) were almost the same in FACE and ambient leaves , indicating that the photosynthetic acclimation is not caused by the decreased Gs . Furthermore , carboxylation efficiency and maximal Pn , both light and CO2-saturated Pn , were decreased in FACE leaves , as shown by the Pn-Ci curves . In addition , the soluble protein , Rubisco ( ribulose-1 , 5-bisphosphate caboxylase/oxygenase ) , and its activase contents as well as the sucrose-phosphate synthase activity decreased significantly , while some soluble sugar , inorganic phosphate , chlorophyll and light-harvesting complex II ( LHC II ) contents increased in FACE leaves . It appears that the photosynthetic acclimation in rice leaves is related to both ribulose-1 , 5-bisphosphate ( RuBP ) carboxylation limitation and RuBP regeneration limitation .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
|---|
| Title: [ Effects of soil water status on gas exchange of peanut and early rice leaves ] | | Author: Chen J L G He Y | | Journal: Citation: V : 16 ( 1 ) P : 105-10 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15852967 Accession (PMID): 15852967 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: The gas exchange characteristics of peanut and early rice leaves were investigated in experimental plots under different soil water conditions over a long growth period . The results showed that at the branching stage of peanut , the stomatal conductance ( Gs ) and transpiration rate ( Tr ) decreased slightly under mild and moderate soil water stress , while the net photosynthetic rate ( Pn ) and leaf water use efficiency ( WUE ) increased . The Gs/Tr ratio also increased under mild water stress , but decreased under moderate water stress . At podding stage , the Gs , Tr , Gs/Tr ratio and Pn decreased , while WUE increased significantly under mild and moderate water stress . The peanut was suffered from water stress at its pod setting stage . At the grain filling stage of early rice , the Gs , Tr and Gs/Tr ratio fluctuated insignificantly under mild and moderate water stress , while Pn and WUE increased significantly , with an increase in grain yield under mild water stress .
[ Sen. 4, subscore: 1.00 ]: The gas exchange characteristics of peanut and early rice leaves were investigated in experimental plots under different soil water conditions over a long growth period . The results showed that at the branching stage of peanut , the stomatal conductance ( Gs ) and transpiration rate ( Tr ) decreased slightly under mild and moderate soil water stress , while the net photosynthetic rate ( Pn ) and leaf water use efficiency ( WUE ) increased . The Gs/Tr ratio also increased under mild water stress , but decreased under moderate water stress . At podding stage , the Gs , Tr , Gs/Tr ratio and Pn decreased , while WUE increased significantly under mild and moderate water stress . The peanut was suffered from water stress at its pod setting stage . At the grain filling stage of early rice , the Gs , Tr and Gs/Tr ratio fluctuated insignificantly under mild and moderate water stress , while Pn and WUE increased significantly , with an increase in grain yield under mild water stress . Its suggested that the combination of Gs and Gs/Tr ratio could be a reference index for crop water stress , namely , crops could be hazarded by water stress when Gs and Gs/Tr decreased synchronously .
[ Sen. 6, subscore: 1.00 ]: The results showed that at the branching stage of peanut , the stomatal conductance ( Gs ) and transpiration rate ( Tr ) decreased slightly under mild and moderate soil water stress , while the net photosynthetic rate ( Pn ) and leaf water use efficiency ( WUE ) increased . The Gs/Tr ratio also increased under mild water stress , but decreased under moderate water stress . At podding stage , the Gs , Tr , Gs/Tr ratio and Pn decreased , while WUE increased significantly under mild and moderate water stress . The peanut was suffered from water stress at its pod setting stage . At the grain filling stage of early rice , the Gs , Tr and Gs/Tr ratio fluctuated insignificantly under mild and moderate water stress , while Pn and WUE increased significantly , with an increase in grain yield under mild water stress . Its suggested that the combination of Gs and Gs/Tr ratio could be a reference index for crop water stress , namely , crops could be hazarded by water stress when Gs and Gs/Tr decreased synchronously .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 4.00 |
|---|
| Title: Is root growth under phosphorus deficiency affected by source or sink limitations?
| | Author: Wissuwa M Gamat G Ismail AM .
| | Journal: J Exp . Bot . Citation: V : 56 ( 417 ) P : 1943-50 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15911558 Accession (PMID): 15911558 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Reduced net photosynthesis ( Pn ) and decreasing shoot and root biomass are typical effects of phosphorus deficiency in plants . Lower biomass accumulation could be the result of reduced Pn ( source limitation ) , but may also be due to direct negative effects of low P availability on growth ( sink limitation ) . Because of the principal importance of root growth for P uptake , this study specifically examined the question whether source or sink limitations were responsible for reduced root growth rates under P deficiency . Rice plants were grown in nutrient solutions with four levels of P supply and at two light treatments and the effect of Pxlight treatments on growth and carbohydrate distribution was observed . Plants had up to 70% higher Pn when grown with natural ( high ) light compared with low light .
[ Sen. 2, subscore: 1.00 ]: Reduced net photosynthesis ( Pn ) and decreasing shoot and root biomass are typical effects of phosphorus deficiency in plants . Lower biomass accumulation could be the result of reduced Pn ( source limitation ) , but may also be due to direct negative effects of low P availability on growth ( sink limitation ) . Because of the principal importance of root growth for P uptake , this study specifically examined the question whether source or sink limitations were responsible for reduced root growth rates under P deficiency . Rice plants were grown in nutrient solutions with four levels of P supply and at two light treatments and the effect of Pxlight treatments on growth and carbohydrate distribution was observed . Plants had up to 70% higher Pn when grown with natural ( high ) light compared with low light . Higher Pn , however , did not lead to additional growth under P deficiency , suggesting that assimilate supply from source leaves to roots was not a limiting factor under P deficiency .
[ Sen. 5, subscore: 1.00 ]: Reduced net photosynthesis ( Pn ) and decreasing shoot and root biomass are typical effects of phosphorus deficiency in plants . Lower biomass accumulation could be the result of reduced Pn ( source limitation ) , but may also be due to direct negative effects of low P availability on growth ( sink limitation ) . Because of the principal importance of root growth for P uptake , this study specifically examined the question whether source or sink limitations were responsible for reduced root growth rates under P deficiency . Rice plants were grown in nutrient solutions with four levels of P supply and at two light treatments and the effect of Pxlight treatments on growth and carbohydrate distribution was observed . Plants had up to 70% higher Pn when grown with natural ( high ) light compared with low light . Higher Pn , however , did not lead to additional growth under P deficiency , suggesting that assimilate supply from source leaves to roots was not a limiting factor under P deficiency . This was supported by observations that root starch concentrations increased in P-deficient roots . The comparison of two genotypes with different tolerance to P deficiency showed that the more tolerant one preferentially distributed P to roots where the additional P stimulated root growth and , ultimately , P uptake . The results therefore suggest that source limitation is of little importance under P deficiency .
[ Sen. 6, subscore: 1.00 ]: Lower biomass accumulation could be the result of reduced Pn ( source limitation ) , but may also be due to direct negative effects of low P availability on growth ( sink limitation ) . Because of the principal importance of root growth for P uptake , this study specifically examined the question whether source or sink limitations were responsible for reduced root growth rates under P deficiency . Rice plants were grown in nutrient solutions with four levels of P supply and at two light treatments and the effect of Pxlight treatments on growth and carbohydrate distribution was observed . Plants had up to 70% higher Pn when grown with natural ( high ) light compared with low light . Higher Pn , however , did not lead to additional growth under P deficiency , suggesting that assimilate supply from source leaves to roots was not a limiting factor under P deficiency . This was supported by observations that root starch concentrations increased in P-deficient roots . The comparison of two genotypes with different tolerance to P deficiency showed that the more tolerant one preferentially distributed P to roots where the additional P stimulated root growth and , ultimately , P uptake . The results therefore suggest that source limitation is of little importance under P deficiency . Even at highly sub-optimal it issue P concentrations of below 0 . 7 mg P g ( -1 ) dry weight , plants were able to produce enough assimilates to sustain growth rates that were directly limited by low P availability .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
|---|
| Title: Ecological risk assessment on a cadmium contaminated soil landfill--a preliminary evaluation based on toxicity tests on local species and site-specific information .
| | Author: Chen CM Liu MC .
| | Journal: Sci .
Total Environ .
Citation: V : 359 ( 1-3 ) P : 120-9 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15964610 Accession (PMID): 15964610 | Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: In 1982 , brown rice from rice paddy fields in Da-Tan , Tau-Yuan , was found to be contaminated with Cd and Pb due to illegal discharges of wastewater , known as the "Cd rice" incidence . Cadmium laden soil was transferred to a constructed landfill in an industrial park 15 years after the incident . Possible leakage of the landfill was suspected by committee members of a supervising board for the remediation process , and a preliminary ecological risk evaluation was requested . A possible risk scenario was that groundwater contamination due to the leachate containing Cd and Pb from the landfill could result in pollution of coastal water , and subsequently produce toxic effects to aquatic organisms . Chemical dissipation in groundwater systems was simulated and short-term chronic toxicity tests on larvae of three local aquatic species were also performed to determine the no-observed adverse-effect concentrations ( NOAECs ) , as well as the predicted no effect concentrations ( PNECs ) , of the two metals in the organisms tested . The hazard quotient ( HQ ) , the ratio of predicted environmental concentrations ( PECs ) to PNECs , was used for risk characterization . A worst-case-scenario calculation showed that the maximum Cd concentration at 60 m and farther downstream from the site in the groundwater system would be 0 . 0028 mg l ( -1 ) with a maximum initial concentration of 0 . 65 mg l ( -1 ) in the leachate , while for Pb , the highest concentration of 0 . 044 mg l ( -1 ) would be reached at a distance of 40 m and farther , which was based on an initial concentration of 4 . 4 mg l ( -1 ) in the leachate ; however , both cases would only occur 80 years after the initiation of leakage . A presumed dilution factor of 100 was used to calculate the PECs based on the maximum initial concentrations . The Cd and Pbs PNECs for three aquatic animals were 0 . 01 to approximately 0 . 1 mg l ( -1 ) , higher than their PECs in coastal water , resulting in HQs less than 1 for the scenario described .
[ Sen. 7, subscore: 1.00 ]: Cadmium laden soil was transferred to a constructed landfill in an industrial park 15 years after the incident . Possible leakage of the landfill was suspected by committee members of a supervising board for the remediation process , and a preliminary ecological risk evaluation was requested . A possible risk scenario was that groundwater contamination due to the leachate containing Cd and Pb from the landfill could result in pollution of coastal water , and subsequently produce toxic effects to aquatic organisms . Chemical dissipation in groundwater systems was simulated and short-term chronic toxicity tests on larvae of three local aquatic species were also performed to determine the no-observed adverse-effect concentrations ( NOAECs ) , as well as the predicted no effect concentrations ( PNECs ) , of the two metals in the organisms tested . The hazard quotient ( HQ ) , the ratio of predicted environmental concentrations ( PECs ) to PNECs , was used for risk characterization . A worst-case-scenario calculation showed that the maximum Cd concentration at 60 m and farther downstream from the site in the groundwater system would be 0 . 0028 mg l ( -1 ) with a maximum initial concentration of 0 . 65 mg l ( -1 ) in the leachate , while for Pb , the highest concentration of 0 . 044 mg l ( -1 ) would be reached at a distance of 40 m and farther , which was based on an initial concentration of 4 . 4 mg l ( -1 ) in the leachate ; however , both cases would only occur 80 years after the initiation of leakage . A presumed dilution factor of 100 was used to calculate the PECs based on the maximum initial concentrations . The Cd and Pbs PNECs for three aquatic animals were 0 . 01 to approximately 0 . 1 mg l ( -1 ) , higher than their PECs in coastal water , resulting in HQs less than 1 for the scenario described . We concluded that if a leakage does occur , the ecological impact would be minimal because a majority of the metals will be retained in the soil phase and the remaining , if released into the aquatic system , can not reach high enough concentrations to produce toxicity to marine animals .
[ Sen. 10, subscore: 1.00 ]: Chemical dissipation in groundwater systems was simulated and short-term chronic toxicity tests on larvae of three local aquatic species were also performed to determine the no-observed adverse-effect concentrations ( NOAECs ) , as well as the predicted no effect concentrations ( PNECs ) , of the two metals in the organisms tested . The hazard quotient ( HQ ) , the ratio of predicted environmental concentrations ( PECs ) to PNECs , was used for risk characterization . A worst-case-scenario calculation showed that the maximum Cd concentration at 60 m and farther downstream from the site in the groundwater system would be 0 . 0028 mg l ( -1 ) with a maximum initial concentration of 0 . 65 mg l ( -1 ) in the leachate , while for Pb , the highest concentration of 0 . 044 mg l ( -1 ) would be reached at a distance of 40 m and farther , which was based on an initial concentration of 4 . 4 mg l ( -1 ) in the leachate ; however , both cases would only occur 80 years after the initiation of leakage . A presumed dilution factor of 100 was used to calculate the PECs based on the maximum initial concentrations . The Cd and Pbs PNECs for three aquatic animals were 0 . 01 to approximately 0 . 1 mg l ( -1 ) , higher than their PECs in coastal water , resulting in HQs less than 1 for the scenario described . We concluded that if a leakage does occur , the ecological impact would be minimal because a majority of the metals will be retained in the soil phase and the remaining , if released into the aquatic system , can not reach high enough concentrations to produce toxicity to marine animals .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Thermostability of photosynthesis in two new chlorophyll b-less rice mutants .
| | Author: Lin Z Peng C Xu X Lin G Zhang J | | Journal: Sci .
China , C , Life Sci .
Citation: V : 48 ( 2 ) P : 139-47 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15986886 Accession (PMID): 15986886 | Matching Sentences:
[ Sen. 7, subscore: 1.00 ]: The thermostability of photosynthetic apparatus was estimated by the changes in chlorophyll fluorescence parameters , photosynthetic rate and pigment content , chloroplast ultrastructure and it issue location of H2O2 accumulation . There were different patterns of F ( o ) -temperature curves between the Chl b-less mutants and the wild type plant , and the temperature of F ( o ) rising threshold was shifted 3 degrees C lower in the Chl b-less mutants ( 48 degrees C ) than in the wild type ( 51 degrees C ) . At temperature up to about 45 degrees C , chloroplasts were swollen and thylakoid grana became misty accompanied with the complete loss of photosynthetic oxygen evolution in the two Chl b-less mutants , but chloroplast ultrastructure in the wild type showed no obvious alteration . After 55 degrees C exposure , the disordered thylakoid and significant H2O2 accumulation in leaves were found in the two Chl b-less mutants , whereas in the wild type plant , less H2O2 was accumulated and the swollen thylakoid still maintained a certain extent of stacking . A large extent of the changes in qP , NPQ and Fv/Fm was consistent with the Pn decreasing rate in the Chl b-less mutants during high temperature treatment as compared with the wild type . The results indicated that the Chl b-less mutants showed a tendency for higher thermosensitivity , and loss of Chl b in LHC II could lead to less thermostability of PSII structure and function . Heat damage to photosynthetic apparatus might be partially attributed to the internal oxidative stress produced at severely high temperature .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: The Chakaria food system study : household-level , case-control study to identify risk factor for rickets in Bangladesh .
| | Author: Combs GF Hassan N | | Journal: Citation: V : 59 ( 11 ) P : 1291-301 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16047028 Accession (PMID): 16047028 | Matching Sentences:
[ Sen. 7, subscore: 1.00 ]: SETTING : A rickets-endemic area of southeastern Bangladesh , Chakaria . SUBJECTS AND METHODS : An interview-based survey was conducted in six villages in Chakaria with 199 households with at least one child showing physical signs consistent with rickets and 281 households with no affected children . RESULTS : Households with rachitic children in Chakaria had more children , more pregnant or lactating women , and fewer adults than unaffected households in that community . Affected households tended to rely on farming for their livelihood and tended to have less economic activity as indicated by less outstanding debt than their neighbors . Households with rickets were at significantly greater risk of pneumonia than were other households . Calcium undernutrition was severe and widespread in Chakaria due to a food system that offered very little of the element in accessible forms . Household diets were based on cereals and starchy vegetables . Rice and fish constituted the major source of calcium for most households , although dairy products , when used , were very important calcium sources , particularly for young children . In fact , the use of dairy products was the only household choice that led to substantial increases in the calcium intakes of children , and households that used dairy products tended to show increased calcium intakes for all of their members .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 5.00 |
|---|
| Title: Identification of an RNA silencing suppressor from a plant double-stranded RNA virus .
| | Author: Cao X Zhou P Zhang X Zhu S Zhong X Xiao Q Ding B Li Y | | Journal: J Virol .
Citation: V : 79 ( 20 ) P : 13018-27 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16189004 Accession (PMID): 16189004 | Matching Sentences:
[ Sen. 8, subscore: 2.00 ]: Pns10 , encoded by S10 of Rice dwarf phytoreovirus ( RDV ) , exhibited RSS activity in coinfiltration assays with the reporter green fluorescent protein ( GFP ) in transgenic Nicotiana benthamiana line 16c carrying GFP . The other gene segments of the RDV genome did not have such a function . Pns10 suppressed local and systemic silencing induced by sense RNA but did not interfere with local and systemic silencing induced by dsRNA . Expression of Pns10 also increased the expression of beta-glucuronidase in transient assays and enhanced Potato virus X pathogenicity in N benthamiana . Collectively , our results establish Pns10 as an RSS encoded by a plant dsRNA virus and further suggest that Pns10 targets an upstream step of dsRNA formation in the RNA silencing pathway .
[ Sen. 4, subscore: 1.00 ]: RNA silencing is a mechanism which higher plants and animals have evolved to defend against viral infection in addition to regulation of gene expression for growth and development . As a counterdefense , many plant and some animal viruses studied to date encode RNA silencing suppressors ( RSS ) that interfere with various steps of the silencing pathway . In this study , we report the first identification of an RSS from a plant double-stranded RNA ( dsRNA ) virus . Pns10 , encoded by S10 of Rice dwarf phytoreovirus ( RDV ) , exhibited RSS activity in coinfiltration assays with the reporter green fluorescent protein ( GFP ) in transgenic Nicotiana benthamiana line 16c carrying GFP . The other gene segments of the RDV genome did not have such a function . Pns10 suppressed local and systemic silencing induced by sense RNA but did not interfere with local and systemic silencing induced by dsRNA . Expression of Pns10 also increased the expression of beta-glucuronidase in transient assays and enhanced Potato virus X pathogenicity in N benthamiana . Collectively , our results establish Pns10 as an RSS encoded by a plant dsRNA virus and further suggest that Pns10 targets an upstream step of dsRNA formation in the RNA silencing pathway .
[ Sen. 6, subscore: 1.00 ]: As a counterdefense , many plant and some animal viruses studied to date encode RNA silencing suppressors ( RSS ) that interfere with various steps of the silencing pathway . In this study , we report the first identification of an RSS from a plant double-stranded RNA ( dsRNA ) virus . Pns10 , encoded by S10 of Rice dwarf phytoreovirus ( RDV ) , exhibited RSS activity in coinfiltration assays with the reporter green fluorescent protein ( GFP ) in transgenic Nicotiana benthamiana line 16c carrying GFP . The other gene segments of the RDV genome did not have such a function . Pns10 suppressed local and systemic silencing induced by sense RNA but did not interfere with local and systemic silencing induced by dsRNA . Expression of Pns10 also increased the expression of beta-glucuronidase in transient assays and enhanced Potato virus X pathogenicity in N benthamiana . Collectively , our results establish Pns10 as an RSS encoded by a plant dsRNA virus and further suggest that Pns10 targets an upstream step of dsRNA formation in the RNA silencing pathway .
[ Sen. 7, subscore: 1.00 ]: In this study , we report the first identification of an RSS from a plant double-stranded RNA ( dsRNA ) virus . Pns10 , encoded by S10 of Rice dwarf phytoreovirus ( RDV ) , exhibited RSS activity in coinfiltration assays with the reporter green fluorescent protein ( GFP ) in transgenic Nicotiana benthamiana line 16c carrying GFP . The other gene segments of the RDV genome did not have such a function . Pns10 suppressed local and systemic silencing induced by sense RNA but did not interfere with local and systemic silencing induced by dsRNA . Expression of Pns10 also increased the expression of beta-glucuronidase in transient assays and enhanced Potato virus X pathogenicity in N benthamiana . Collectively , our results establish Pns10 as an RSS encoded by a plant dsRNA virus and further suggest that Pns10 targets an upstream step of dsRNA formation in the RNA silencing pathway .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Grain yield responses to moisture regimes in a rice population : association among traits and genetic markers .
| | Author: Zou GH Mei HW Liu HY Liu GL Hu SP Yu XQ Li MS Wu JH Luo LJ .
| | Journal: Theor . Appl . Genet . Citation: V : 112 ( 1 ) P : 106-13 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16231161 Accession (PMID): 16231161 | Matching Sentences:
[ Sen. 7, subscore: 1.00 ]: Grain yield ( GY ) and its components of a recombinant inbred population developed from a lowland rice and an upland rice were investigated under different water levels in 2003 and 2004 in a rainout DT screening facility . Correlation and path analysis indicated that spikelet fertility ( SF ) was particularly important for grain yield with direct effect ( P=0 . 60 ) under drought stress , while spikelet number per panicle ( SN ) contributed the most to grain yield ( P=0 . 41 ) under well-watered condition . A total of 32 quantitative trait loci ( QTLs ) for grain yield and its components were identified . The phenotypic variation explained by individual QTLs varied from 1 . 29% to 14 . 76% . Several main effect QTLs affecting SF , 1 , 000-grain weight ( TGW ) , panicle number ( PN ) , and SN were mapped to the same regions on chromosome 4 and 8 . These QTLs were detected consistently across 2 years and under both water levels in this study . Several digenic interactions among yield components were also detected . The identification of genomic regions associated with GY and its components under stress will be useful to improve drought tolerance of rice by marker-aided approaches .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 6.00 |
|---|
| Title: Pns12 protein of Rice dwarf virus is essential for formation of viroplasms and nucleation of viral-assembly complexes .
| | Author: Wei T Shimizu T Hagiwara K Kikuchi A Moriyasu Y Suzuki N Chen H Omura T | | Journal: J Gen . Virol . Citation: V : 87 ( Pt 2 ) P : 429-38 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16432031 Accession (PMID): 16432031 | Matching Sentences:
[ Sen. 3, subscore: 3.00 ]: Cytoplasmic inclusion bodies , known as viroplasms or viral factories , are assumed to be the sites of replication of members of the family Reoviridae . Immunocytochemical and biochemical analyses were carried out to characterize the poorly understood viroplasms of the phytoreovirus Rice dwarf virus ( RDV ) . Within 6 h of inoculation of cells , viroplasms , namely discrete cytoplasmic inclusions , were formed that contained the non-structural proteins Pns6 , Pns11 and Pns12 of RDV , which appeared to be the constituents of the inclusions . Formation of similar inclusions in non-host insect cells upon expression of Pns12 in a baculovirus system and the association of molecules of Pns12 in vitro suggested that the inclusions observed in RDV-infected cells were composed basically of Pns12 . Core proteins P1 , P3 , P5 and P7 and core virus particles were identified in the interior region of the inclusions . In contrast , accumulation of the outer capsid proteins P2 , P8 and P9 and of intact virus particles was evident in the peripheral regions of the inclusions . These observations suggest that core particles were constructed inside the inclusions , whereas outer capsid proteins were assembled at the periphery of the inclusions .
[ Sen. 4, subscore: 3.00 ]: Cytoplasmic inclusion bodies , known as viroplasms or viral factories , are assumed to be the sites of replication of members of the family Reoviridae . Immunocytochemical and biochemical analyses were carried out to characterize the poorly understood viroplasms of the phytoreovirus Rice dwarf virus ( RDV ) . Within 6 h of inoculation of cells , viroplasms , namely discrete cytoplasmic inclusions , were formed that contained the non-structural proteins Pns6 , Pns11 and Pns12 of RDV , which appeared to be the constituents of the inclusions . Formation of similar inclusions in non-host insect cells upon expression of Pns12 in a baculovirus system and the association of molecules of Pns12 in vitro suggested that the inclusions observed in RDV-infected cells were composed basically of Pns12 . Core proteins P1 , P3 , P5 and P7 and core virus particles were identified in the interior region of the inclusions . In contrast , accumulation of the outer capsid proteins P2 , P8 and P9 and of intact virus particles was evident in the peripheral regions of the inclusions . These observations suggest that core particles were constructed inside the inclusions , whereas outer capsid proteins were assembled at the periphery of the inclusions . Viral inclusions were shown to be the sites of viral RNA synthesis by labelling infected cells with 5-bromouridine 5-triphosphate .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Substitution mapping of dth1 . 1 , a flowering-time quantitative trait locus ( QTL ) associated with transgressive variation in rice , reveals multiple sub-QTL .
| | Author: Thomson MJ Edwards JD Septiningsih EM Harrington SE McCouch SR .
| | Journal: Genetics Citation: V : 172 ( 4 ) P : 2501-14 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16452146 Accession (PMID): 16452146 | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: A quantitative trait locus ( QTL ) , dth1 . 1 , was associated with transgressive variation for days to heading in an advanced backcross population derived from the Oryza sativa variety Jefferson and an accession of the wild rice relative Oryza rufipogon . A series of near-isogenic lines ( NILs ) containing different O rufipogon introgressions across the target region were constructed to dissect dth1 . 1 using substitution mapping . In contrast to the late-flowering O rufipogon parent , O rufipogon alleles in the substitution lines caused early flowering under both short and long-day lengths and provided evidence for at least two distinct sub-QTL : dth1 . 1a and dth1 . 1b . Potential candidate genes underlying these sub-QTL include genes with sequence similarity to Arabidopsis GI , FT , SOC1 , and EMF1 , and Pharbitis nil PNZIP . Evidence from families with nontarget O rufipogon introgressions in combination with dth1 . 1 alleles also detected an early flowering QTL on chromosome 4 and a late-flowering QTL on chromosome 6 and provided evidence for additional sub-QTL in the dth1 . 1 region . The availability of a series of near-isogenic lines with alleles introgressed from a wild relative of rice provides an opportunity to better understand the molecular basis of transgressive variation in a quantitative trait .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
|---|
| Title: Identification of nif genes in N2-fixing bacterial strains isolated from rice fields along the Yangtze River Plain .
| | Author: Xie GH Cui Z Yu J Yan J Hai W Steinberger Y | | Journal: J Basic Microbiol .
Citation: V : 46 ( 1 ) P : 56-63 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16463319 Accession (PMID): 16463319 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: The aim of this research was to identify nifH and nifHDKYE genes in twenty strains of N2-fixing heterotrophic bacteria isolated from rice fields in the Yangtze River Plain . Southern hybridization of the total DNA from each strain was performed with the Klebsiella pneumoniae nifHDKYE gene probe ( 6 . 2 kb Eco RI fragment from pSA30 ) and the Azospirillum brasilense nifH gene probe ( 0 . 6 kb Eco RI-Hin dIII fragment from pHU8 ) . We found that Eco RI fragments of total DNA from Aeromonas hydrophila HY2 , Bacillus azotoformans FD , Bacillus licheniformis NCH1 , NCH5 , WH4 , Bacillus brevis NC2 , Bacillus pumilus NC12 , Bacillus cereus NCH2 , Citrobacter freundii HY5 , HY9 , Derxia gummosa HZ5 , Pseudomonas mendocina HZ1 and Pseudomonas pseudoalcaligenes WH3 were positively hybridized with both of the probes . Agrobacterium radiobacter HY17 , Corynebacterium sp . HY12 , YZ and Pseudomonas sp . HY11 had Eco RI fragments hybridized with the K pneumoniae nifHDKYE gene probe .
[ Sen. 6, subscore: 1.00 ]: Southern hybridization of the total DNA from each strain was performed with the Klebsiella pneumoniae nifHDKYE gene probe ( 6 . 2 kb Eco RI fragment from pSA30 ) and the Azospirillum brasilense nifH gene probe ( 0 . 6 kb Eco RI-Hin dIII fragment from pHU8 ) . We found that Eco RI fragments of total DNA from Aeromonas hydrophila HY2 , Bacillus azotoformans FD , Bacillus licheniformis NCH1 , NCH5 , WH4 , Bacillus brevis NC2 , Bacillus pumilus NC12 , Bacillus cereus NCH2 , Citrobacter freundii HY5 , HY9 , Derxia gummosa HZ5 , Pseudomonas mendocina HZ1 and Pseudomonas pseudoalcaligenes WH3 were positively hybridized with both of the probes . Agrobacterium radiobacter HY17 , Corynebacterium sp . HY12 , YZ and Pseudomonas sp . HY11 had Eco RI fragments hybridized with the K pneumoniae nifHDKYE gene probe . An Eco RI fragment of total DNA from Bacillus megaterium YY4 was positively hybridized to the A brasilense nifH gene probe . No hybridization sign was found in the total DNA fragments from Alcaligenes cupidus YY6 and Corynebacterium sp . NC11 hybridized with either of the gene probes . The data provide the number and size of EcoRI fragments of the total DNA hybridized with the nif gene probes for these strains of rarely studied species , suggesting additional evidence for N2 fixing and nif gene diversity of N2-fixing bacteria in rice fields along the Yangtze River Plain .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
|---|
| Title: Photosynthetic light and CO2 utilization and C4 traits of two novel super-rice hybrids .
| | Author: Wang Q Zhang Q Fan D Lu C | | Journal: J Plant Physiol .
Citation: V : 163 ( 5 ) P : 529-37 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16473657 Accession (PMID): 16473657 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Characteristics of photosynthetic light and CO2 use efficiency from seedling to heading stage , and C4 pathway enzyme activities in both flag leaves and lemma were compared between two newly developed super-rice hybrids ( Oryza sativa L ) , Liangyoupeijiu and Hua-an 3 , and a traditional rice hybrid , Shanyou 63 . At seedling and tillering stages , Liangyoupeijiu and Hua-an 3 had higher net photosynthetic rates ( Pn ) and light saturated assimilation rates ( Asat ) than did Shanyou 63 , at both normal ( 360 micromol mol ( -1 ) ) and doubled ( 720 micromol mol ( -1 ) ) CO2 concentrations . At the heading stage , the flag leaves of all three rice hybrids had similar Pn and Asat . However , the two super-rice hybrids had higher apparent quantum yield ( AQY ) and carboxylation efficiency ( CE ) during all three typical developmental stages , and higher quantum yield of CO2 fixation ( PhiCO2 ) at the tillering and heading stages . In addition , Liangyoupeijiu showed significantly higher activities of the C ( 4 ) pathway enzymes in both flag leaves and lemmas than did Shanyou 63 . As a result , flag leaves of the two super-rice hybrids had higher Pn at morning , noontime and late afternoon during the daily cycle .
[ Sen. 3, subscore: 1.00 ]: Characteristics of photosynthetic light and CO2 use efficiency from seedling to heading stage , and C4 pathway enzyme activities in both flag leaves and lemma were compared between two newly developed super-rice hybrids ( Oryza sativa L ) , Liangyoupeijiu and Hua-an 3 , and a traditional rice hybrid , Shanyou 63 . At seedling and tillering stages , Liangyoupeijiu and Hua-an 3 had higher net photosynthetic rates ( Pn ) and light saturated assimilation rates ( Asat ) than did Shanyou 63 , at both normal ( 360 micromol mol ( -1 ) ) and doubled ( 720 micromol mol ( -1 ) ) CO2 concentrations . At the heading stage , the flag leaves of all three rice hybrids had similar Pn and Asat . However , the two super-rice hybrids had higher apparent quantum yield ( AQY ) and carboxylation efficiency ( CE ) during all three typical developmental stages , and higher quantum yield of CO2 fixation ( PhiCO2 ) at the tillering and heading stages . In addition , Liangyoupeijiu showed significantly higher activities of the C ( 4 ) pathway enzymes in both flag leaves and lemmas than did Shanyou 63 . As a result , flag leaves of the two super-rice hybrids had higher Pn at morning , noontime and late afternoon during the daily cycle . Since most of the grain yield of rice comes from the photosynthesis of flag leaves , the similar Asat and much higher AQY , CE and PhiCO2 at heading stage of the two super-rice hybrids indicates that higher photosynthetic efficiency rather than higher photosynthetic capacity may be the primary factor contributing to their higher grain yields .
[ Sen. 6, subscore: 1.00 ]: At seedling and tillering stages , Liangyoupeijiu and Hua-an 3 had higher net photosynthetic rates ( Pn ) and light saturated assimilation rates ( Asat ) than did Shanyou 63 , at both normal ( 360 micromol mol ( -1 ) ) and doubled ( 720 micromol mol ( -1 ) ) CO2 concentrations . At the heading stage , the flag leaves of all three rice hybrids had similar Pn and Asat . However , the two super-rice hybrids had higher apparent quantum yield ( AQY ) and carboxylation efficiency ( CE ) during all three typical developmental stages , and higher quantum yield of CO2 fixation ( PhiCO2 ) at the tillering and heading stages . In addition , Liangyoupeijiu showed significantly higher activities of the C ( 4 ) pathway enzymes in both flag leaves and lemmas than did Shanyou 63 . As a result , flag leaves of the two super-rice hybrids had higher Pn at morning , noontime and late afternoon during the daily cycle . Since most of the grain yield of rice comes from the photosynthesis of flag leaves , the similar Asat and much higher AQY , CE and PhiCO2 at heading stage of the two super-rice hybrids indicates that higher photosynthetic efficiency rather than higher photosynthetic capacity may be the primary factor contributing to their higher grain yields .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 4.00 |
|---|
| Title: Pns4 of rice dwarf virus is a phosphoprotein , is localized around the viroplasm matrix , and forms minitubules .
| | Author: Wei T Kikuchi A Suzuki N Shimizu T Hagiwara K Chen H Omura T | | Journal: Arch . Virol . Citation: V : 151 ( 9 ) P : 1701-12 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16609816 Accession (PMID): 16609816 | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: Rice dwarf virus ( RDV ) , a member of the family Reoviridae , has a 12-segmented dsRNA genome . Seven segments , designated S1 , S2 , S3 , S5 , S7 , S8 , and S9 , encode structural proteins , while the remainder encode nonstructural proteins . One of the nonstructural proteins , Pns4 , which is encoded by S4 , was characterized . Pns4 was a phosphorylatable substrate in a phosphorylation assay in vivo ; it associated with large cytoplasmic fibrils and formed novel minitubules in infected cultured cells of its leafhopper insect vector , as revealed by immunofluorescence and immunoelectron microscopy . Early in infection , Pns4 was detected at the periphery of the viroplasm , and it was then observed on amorphous or fibrillar inclusions , which were identified as bundles of minitubules , at later stages of infection . Since viroplasms are believed to be the site of RDV replication , the intracellular location of Pns4 suggests that this protein might be involved in the process of assembly of the RDV virion .
[ Sen. 4, subscore: 1.00 ]: Rice dwarf virus ( RDV ) , a member of the family Reoviridae , has a 12-segmented dsRNA genome . Seven segments , designated S1 , S2 , S3 , S5 , S7 , S8 , and S9 , encode structural proteins , while the remainder encode nonstructural proteins . One of the nonstructural proteins , Pns4 , which is encoded by S4 , was characterized . Pns4 was a phosphorylatable substrate in a phosphorylation assay in vivo ; it associated with large cytoplasmic fibrils and formed novel minitubules in infected cultured cells of its leafhopper insect vector , as revealed by immunofluorescence and immunoelectron microscopy . Early in infection , Pns4 was detected at the periphery of the viroplasm , and it was then observed on amorphous or fibrillar inclusions , which were identified as bundles of minitubules , at later stages of infection . Since viroplasms are believed to be the site of RDV replication , the intracellular location of Pns4 suggests that this protein might be involved in the process of assembly of the RDV virion .
[ Sen. 5, subscore: 1.00 ]: Rice dwarf virus ( RDV ) , a member of the family Reoviridae , has a 12-segmented dsRNA genome . Seven segments , designated S1 , S2 , S3 , S5 , S7 , S8 , and S9 , encode structural proteins , while the remainder encode nonstructural proteins . One of the nonstructural proteins , Pns4 , which is encoded by S4 , was characterized . Pns4 was a phosphorylatable substrate in a phosphorylation assay in vivo ; it associated with large cytoplasmic fibrils and formed novel minitubules in infected cultured cells of its leafhopper insect vector , as revealed by immunofluorescence and immunoelectron microscopy . Early in infection , Pns4 was detected at the periphery of the viroplasm , and it was then observed on amorphous or fibrillar inclusions , which were identified as bundles of minitubules , at later stages of infection . Since viroplasms are believed to be the site of RDV replication , the intracellular location of Pns4 suggests that this protein might be involved in the process of assembly of the RDV virion .
[ Sen. 6, subscore: 1.00 ]: Seven segments , designated S1 , S2 , S3 , S5 , S7 , S8 , and S9 , encode structural proteins , while the remainder encode nonstructural proteins . One of the nonstructural proteins , Pns4 , which is encoded by S4 , was characterized . Pns4 was a phosphorylatable substrate in a phosphorylation assay in vivo ; it associated with large cytoplasmic fibrils and formed novel minitubules in infected cultured cells of its leafhopper insect vector , as revealed by immunofluorescence and immunoelectron microscopy . Early in infection , Pns4 was detected at the periphery of the viroplasm , and it was then observed on amorphous or fibrillar inclusions , which were identified as bundles of minitubules , at later stages of infection . Since viroplasms are believed to be the site of RDV replication , the intracellular location of Pns4 suggests that this protein might be involved in the process of assembly of the RDV virion .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
|---|
| Title: Identification of related QTLs at late developmental stage in rice ( Oryza sativa L ) under two nitrogen levels .
| | Author: Tong HH Mei HW Yu XQ Xu XY Li MS Zhang SQ Luo LJ .
| | Journal: Yi Chuan Xue Bao Citation: V : 33 ( 5 ) P : 458-67 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16722341 Accession (PMID): 16722341 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: QTL underlying related traits at the late developmental stage under two different nitrogen levels were investigated in rice using a population of chromosome segment substitution lines ( CSSL ) derived from a cross between Teqing and Lemont . A total of 31 QTLs referring 5 traits , that is , plant height ( PH ) , panicle number per plant ( PN ) , chlorophyll content ( CC ) , shoot dry weight ( SDW ) and grain yield per plant ( YD ) , were detected . Under normal N level , 3 QTLs were detected for each trait , while under low N level , 5 , 4 , 5 and 2 QTLs were detected for PH , PN , CC and SDW respectively . Most of the QTLs were located on chromosome 2 , 3 , 7 , 11 and 12 . QTLs controlling different traits or the same trait under different N levels were mapped on the same or adjacent intervals , forming several clusters in rice chromosomes . More than two traits were controlled by QTLs on one of four intervals ( RM30-RM439 , RM18-RM478 , RM309-RM270 , and RM235-RM17 ) , suggesting that there were some pleiotropic effects .
[ Sen. 3, subscore: 1.00 ]: QTL underlying related traits at the late developmental stage under two different nitrogen levels were investigated in rice using a population of chromosome segment substitution lines ( CSSL ) derived from a cross between Teqing and Lemont . A total of 31 QTLs referring 5 traits , that is , plant height ( PH ) , panicle number per plant ( PN ) , chlorophyll content ( CC ) , shoot dry weight ( SDW ) and grain yield per plant ( YD ) , were detected . Under normal N level , 3 QTLs were detected for each trait , while under low N level , 5 , 4 , 5 and 2 QTLs were detected for PH , PN , CC and SDW respectively . Most of the QTLs were located on chromosome 2 , 3 , 7 , 11 and 12 . QTLs controlling different traits or the same trait under different N levels were mapped on the same or adjacent intervals , forming several clusters in rice chromosomes . More than two traits were controlled by QTLs on one of four intervals ( RM30-RM439 , RM18-RM478 , RM309-RM270 , and RM235-RM17 ) , suggesting that there were some pleiotropic effects . It was supposed that some QTLs only detected at low N level might be associated with the ability to tolerate the low N stress in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: [ The inductive activation of the promoter of pib gene ] | | Author: Li CJ Yang SH Wu L Wan JM .
| | Journal: Yi Chuan Citation: V : 28 ( 6 ) P : 689-94 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16818431 Accession (PMID): 16818431 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: A 5 . 7 kb putative promoter region of pib gene was isolated from the pib genomic clone and substituted for the 35S promoter upstream of gus gene in plasmid pCAMBIA1301 to construct a new plant expression vector pNAR604 ( putative pib promoter-GUS + 35S-hpt ) . From Agrobacterium-mediated transformation and hygromycin selective culture in vitro , hygromycin resistant calli and 36 transgenic rice ( Oryza sativa L ) plants were obtained . Histochemical assays of GUS activity showed that no expression was observed in the resistant calli and roots from transgenic rice if cultured under light , but after 24 h dark treatment there was strong GUS staining . Fluorimetric quantitative analysis indicated that GUS expression was organ-specific in transgenic rice . Without the dark treatment , GUS activity in roots and stems were about 7 and 3 times higher than in leaves in which GUS activity was only trace detected .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: In vitro bactericidal activities of Japanese rice-fluid against Helicobacter pylori strains .
| | Author: Kawakami Y Oana K Hayama M Ota H Takeuchi M Miyashita K Matsuzawa T Kanaya K | | Journal: Citation: V : 3 ( 3 ) P : 112-6 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16906220 Accession (PMID): 16906220 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: BACKGROUND : Helicobacter pylori has now been widely recognized as a causative agent of gastroduodenal diseases . The development of safer anti H pylori compounds is desirable due to the antibiotic-resistant strains emerged to date . METHODS : We successfully developed the compounds of Rice-fluid derived from unpolished , polished , and usually cooked Japanese rice , and investigated their in vitro antibacterial activities by means of the Time-Kill-Curve methods against various species of bacteria including H pylori strains . RESULTS : All of the compounds revealed keen bactericidal activities against H pylori , followed by Streptococcus pneumoniae and Campylobacter jejuni strains , but failed to affect the viability of other bacterial species investigated including staphylococci , enterococci , Pseudomonas aeruginosa , and other gram-negative rods belonging to the family Enterobacteraceae . The bactericidal activities were demonstrated to be time and concentration-dependent . CONCLUSIONS : The compounds of Rice-fluid are considered to be potentially new and safe therapeutic regimens against H pylori infections . The mechanism of their bactericidal activities against H pylori strains remains to be elucidated .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 4.00 |
|---|
| Title: The spread of Rice dwarf virus among cells of its insect vector exploits virus-induced tubular structures .
| | Author: Wei T Kikuchi A Moriyasu Y Suzuki N Shimizu T Hagiwara K Chen H Takahashi M Ichiki-Uehara T Omura T | | Journal: J Virol .
Citation: V : 80 ( 17 ) P : 8593-602 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16912308 Accession (PMID): 16912308 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Various cytopathological structures , known as inclusion bodies , are formed upon infection of cultured leafhopper cells by Rice dwarf virus , a member of the family Reoviridae . These structures include tubules of approximately 85 nm in diameter which are composed of the nonstructural viral protein Pns10 and contain viral particles . Such tubular structures were produced in heterologous non-host insect cells that expressed Pns10 of the virus . These tubules , when associated with actin-based filopodia , were able to protrude from the surface of cells and to penetrate neighboring cells . A binding assay in vitro revealed the specific binding of Pns10 to actin . Infection of clusters of cells was readily apparent 5 days after inoculation at a low multiplicity of infection with the virus , even in the presence of neutralizing antibodies .
[ Sen. 3, subscore: 1.00 ]: Various cytopathological structures , known as inclusion bodies , are formed upon infection of cultured leafhopper cells by Rice dwarf virus , a member of the family Reoviridae . These structures include tubules of approximately 85 nm in diameter which are composed of the nonstructural viral protein Pns10 and contain viral particles . Such tubular structures were produced in heterologous non-host insect cells that expressed Pns10 of the virus . These tubules , when associated with actin-based filopodia , were able to protrude from the surface of cells and to penetrate neighboring cells . A binding assay in vitro revealed the specific binding of Pns10 to actin . Infection of clusters of cells was readily apparent 5 days after inoculation at a low multiplicity of infection with the virus , even in the presence of neutralizing antibodies . However , treatment of host cells with drugs that inhibited the elongation of actin filaments abolished the extension of Pns10 tubules from the surface of cells , with a significant simultaneous decrease in the extent of infection of neighboring cells .
[ Sen. 5, subscore: 1.00 ]: Various cytopathological structures , known as inclusion bodies , are formed upon infection of cultured leafhopper cells by Rice dwarf virus , a member of the family Reoviridae . These structures include tubules of approximately 85 nm in diameter which are composed of the nonstructural viral protein Pns10 and contain viral particles . Such tubular structures were produced in heterologous non-host insect cells that expressed Pns10 of the virus . These tubules , when associated with actin-based filopodia , were able to protrude from the surface of cells and to penetrate neighboring cells . A binding assay in vitro revealed the specific binding of Pns10 to actin . Infection of clusters of cells was readily apparent 5 days after inoculation at a low multiplicity of infection with the virus , even in the presence of neutralizing antibodies . However , treatment of host cells with drugs that inhibited the elongation of actin filaments abolished the extension of Pns10 tubules from the surface of cells , with a significant simultaneous decrease in the extent of infection of neighboring cells . These results together revealed a previously undescribed aspect of the intercellular spread of Rice dwarf virus , wherein the virus exploits tubules composed of a nonstructural viral protein and actin-based filopodia to move into neighboring cells .
[ Sen. 7, subscore: 1.00 ]: Such tubular structures were produced in heterologous non-host insect cells that expressed Pns10 of the virus . These tubules , when associated with actin-based filopodia , were able to protrude from the surface of cells and to penetrate neighboring cells . A binding assay in vitro revealed the specific binding of Pns10 to actin . Infection of clusters of cells was readily apparent 5 days after inoculation at a low multiplicity of infection with the virus , even in the presence of neutralizing antibodies . However , treatment of host cells with drugs that inhibited the elongation of actin filaments abolished the extension of Pns10 tubules from the surface of cells , with a significant simultaneous decrease in the extent of infection of neighboring cells . These results together revealed a previously undescribed aspect of the intercellular spread of Rice dwarf virus , wherein the virus exploits tubules composed of a nonstructural viral protein and actin-based filopodia to move into neighboring cells .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
|---|
| Title: An inventory of N ( 2 ) O emissions from agriculture in China using precipitation-rectified emission factor and background emission .
| | Author: Lu Y Huang Y Zou J Zheng X | | Journal: Chemosphere Citation: V : 65 ( 11 ) P : 1915-24 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16949128 Accession (PMID): 16949128 | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: Fertilized agricultural soils are a major anthropogenic source of atmospheric N ( 2 ) O A credible national inventory of agricultural N ( 2 ) O emission would benefit its global strength estimate . We compiled a worldwide database of N ( 2 ) O emissions from fertilized fields that were consecutively measured for more than or close to one year . Both nitrogen input ( N ) and precipitation ( P ) were found to be largely responsible for temporal and spatial variabilities in annual N ( 2 ) O fluxes ( N ( 2 ) O-N ) . Thus , we established an empirical model ( N ( 2 ) O-N=1 . 49 P+0 . 0186 PN ) , in which both emission factor and background emission for N ( 2 ) O were rectified by precipitation . In this model , annual N ( 2 ) O emission consists of a background emission of 1 . 49 P and a fertilizer-induced emission of 0 . 0186 PN We used this model to develop a spatial inventory at the 10x10km scale of direct N ( 2 ) O emissions from agriculture in China . N ( 2 ) O emissions from rice paddies were separately quantified using a cropping-specific emission factor . Annual fertilizer-induced N ( 2 ) O emissions amounted to 198 . 89GgN ( 2 ) O-N in 1997 , consisting of 18 . 50GgN ( 2 ) O-N from rice paddies and 180 . 39GgN ( 2 ) O-N from fertilized uplands . Annual background emissions and total emissions of N ( 2 ) O from agriculture were estimated to be 92 . 78GgN ( 2 ) O-N and 291 . 67GgN ( 2 ) O-N , respectively .
[ Sen. 5, subscore: 1.00 ]: Fertilized agricultural soils are a major anthropogenic source of atmospheric N ( 2 ) O A credible national inventory of agricultural N ( 2 ) O emission would benefit its global strength estimate . We compiled a worldwide database of N ( 2 ) O emissions from fertilized fields that were consecutively measured for more than or close to one year . Both nitrogen input ( N ) and precipitation ( P ) were found to be largely responsible for temporal and spatial variabilities in annual N ( 2 ) O fluxes ( N ( 2 ) O-N ) . Thus , we established an empirical model ( N ( 2 ) O-N=1 . 49 P+0 . 0186 PN ) , in which both emission factor and background emission for N ( 2 ) O were rectified by precipitation . In this model , annual N ( 2 ) O emission consists of a background emission of 1 . 49 P and a fertilizer-induced emission of 0 . 0186 PN We used this model to develop a spatial inventory at the 10x10km scale of direct N ( 2 ) O emissions from agriculture in China . N ( 2 ) O emissions from rice paddies were separately quantified using a cropping-specific emission factor . Annual fertilizer-induced N ( 2 ) O emissions amounted to 198 . 89GgN ( 2 ) O-N in 1997 , consisting of 18 . 50GgN ( 2 ) O-N from rice paddies and 180 . 39GgN ( 2 ) O-N from fertilized uplands . Annual background emissions and total emissions of N ( 2 ) O from agriculture were estimated to be 92 . 78GgN ( 2 ) O-N and 291 . 67GgN ( 2 ) O-N , respectively . The annual direct N ( 2 ) O emission accounted for 0 . 92% of the applied N with an uncertainty of 29% .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
