423 matches found in 290 documents. Search time: 0.26 seconds. |
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Score: 1.00 | Title: A "defeated" rice resistance gene acts as a QTL against a virulent strain of Xanthomonas oryzae pv . oryzae .
| Author: Li ZK Luo LJ Mei HW Paterson AH Zhao XH Zhong DB Wang YP Yu XQ Zhu L Tabien R Stansel JW Ying CS .
| Journal: Mol . Gen . Genet . Citation: V : 261 ( 1 ) P : 58-63 Year: 1999 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub10071210 Accession (PMID): 10071210 | Matching Sentences: [ Sen. 1, subscore: 1.00 ]: The genetic components responsible for qualitative and quantitative resistance of rice plants to three strains ( CR4 , CXO8 , and CR6 ) of Xanthomonas oryzae pv . oryzae ( Xoo ) were investigated using a set of 315 recombinant inbred lines ( RILs ) from the cross Lemont ( japonica ) x Teqing ( indica ) and a complete linkage map with 182 well distributed RFLP markers . We mapped a major gene ( Xa4 ) and ten quantitative trait loci ( QTLs ) which were largely responsible for segregation of the resistance phenotype in the RILs . The Teqing allele at the Xa4 locus , Xa4T , acted as a dominant resistance gene against CR4 and CXO8 . The breakdown of Xa4T-associated resistance mediated by the mutant allele at the avrXa4 locus in the virulent strain CR6 results from significant changes in both gene action ( lose of dominance ) and the magnitude of gene effect ( approximately 50% reduction ) . Nevertheless , Xa4T still acted as a recessive QTL with a significant residual effect against CR6 . The mutant alleles at the avrXa4 locus in CXO8 and CR6 that lead to a reduction in effect , or "breakdown" , of Xa4T were apparently accompanied by corresponding penalties for their fitness . The quantitative component of resistance to Xoo in the RILs was largely due to a number of resistance QTLs . Most resistance QTLs mapped to genomic locations where major resistance genes and/or QTLs for resistance to Xoo , blast and sheath blight were identified in the same cross . Most QTLs showed consistent levels of resistance against all three Xoo strains . Our results suggest that a high level of durable resistance to Xoo may be achieved by the cumulative effects of multiple QTLs , including the residual effects of "defeated" major resistance genes .
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Score: 1.00 | Title: Application of restriction fragment fingerprinting with a rice microsatellite sequence to assembling rice YAC clones .
| Author: Ashikawa I Kurata N Saji S Umehara Y Sasaki T | Journal: Genome Citation: V : 42 ( 2 ) P : 330-7 Year: 1999 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub10231964 Accession (PMID): 10231964 | Matching Sentences: [ Sen. 6, subscore: 1.00 ]: To refine the current physical map of rice , we have established a restriction fragment fingerprinting method for identifying overlap between pairs of rice yeast artificial chromosome ( YAC ) clones and defining the physical arrangement of YACs within contiguous fragments ( contigs ) . In this method , Southern blots of rice YAC DNAs digested with a restriction endonuclease are probed with a rice microsatellite probe , ( GGC ) 5 . The probe produces a unique fingerprint profile characteristic of each YAC clone . The profile is then digitized , processed in a computer , and a statistic that represents the degree of overlap between two YACs is calculated . The statistics have been used to detect overlaps among YAC clones , thereby filling a gap between two neighbouring contigs and organizing overlapping rice YAC clones into contiguous fragments . We applied this method to rearranging YACs that had previously been assigned to rice chromosome 6 by anchoring with RFLP markers .
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Score: 2.00 | Title: Identification of mutable slender glume gene in rice ( Oryza sativa L ) .
| Author: Teraishi M Okumoto Y Hirochika H Horibata A Yamagata H Tanisaka T | Journal: Mol . Gen . Genet . Citation: V : 261 ( 3 ) P : 487-94 Year: 1999 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub10323229 Accession (PMID): 10323229 | Matching Sentences: [ Sen. 6, subscore: 2.00 ]: The segregation pattern and chromosomal location of a slender glume mutation , induced by gamma-ray irradiation , was investigated . The mutation is genetically unstable : in the selfed progenies of slender glumed plants , not only plants with normal glumes but also plants that are chimeric for glume shape almost always appear at low frequency . The results showed that the mutation is controlled by a single recessive , mutable mutant gene slg . The frequency of reversion of slg to its wild-type state was little affected by crossing , back-crossing , genetic background or cytoplasmic factors . Conventional trisomic and linkage analyses revealed that the slg locus was located close to the rfs ( rolled fine stripe leaf ) locus on chromosome 7 . In a subsequent RFLP analysis , slg was found to be located between the two RFLP loci XNpb20 and XNpb33 , with recombination values of 3 . 0 and 3 . 2% , respectively . Southern analysis indicated that the mutability of slg is caused by none of the known transposable elements in rice . From these results , we infer that slg has a novel transposable DNA insert in its vicinity , which was possibly activated by gamma-ray irradiation .
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Score: 2.00 | Title: RAPD mapping in a doubled haploid population of rice ( Oryza sativa L ) .
| Author: Subudhi PK Huang N | Journal: Hereditas Citation: V : 130 ( 1 ) P : 41-9 Year: Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub10364828 Accession (PMID): 10364828 | Matching Sentences: [ Sen. 4, subscore: 1.00 ]: To examine the distribution and genome coverage of RAPDs , a total of 242 Random Amplified Polymorphic DNA ( RAPD ) markers generated by 73 random decamer primers were mapped onto 12 rice chromosomes by linkage analysis using a doubled haploid population , developed from an indica x japonica cross . The RAPD markers were derived from both parents equally and were well distributed over the rice genome . Furthermore , multiple RAPD markers generated from the same primer were dispersed over different chromosomes rather than clustered . The RAPD technique provided improved marker coverage on a previously developed RFLP map . A set of primers producing reproducible markers originating from either parent and equally spaced over all the 12 chromosomes were selected for application in marker-assisted backcross breeding . The RAPD analysis as a realistic and practical alternative to RFLP and their usefulness in anchoring the identified BAC contigs directly to chromosomes is discussed . [ Sen. 6, subscore: 1.00 ]: To examine the distribution and genome coverage of RAPDs , a total of 242 Random Amplified Polymorphic DNA ( RAPD ) markers generated by 73 random decamer primers were mapped onto 12 rice chromosomes by linkage analysis using a doubled haploid population , developed from an indica x japonica cross . The RAPD markers were derived from both parents equally and were well distributed over the rice genome . Furthermore , multiple RAPD markers generated from the same primer were dispersed over different chromosomes rather than clustered . The RAPD technique provided improved marker coverage on a previously developed RFLP map . A set of primers producing reproducible markers originating from either parent and equally spaced over all the 12 chromosomes were selected for application in marker-assisted backcross breeding . The RAPD analysis as a realistic and practical alternative to RFLP and their usefulness in anchoring the identified BAC contigs directly to chromosomes is discussed .
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Score: 2.00 | Title: Isolation and characterization of rice MADS box gene homologues and their RFLP mapping .
| Author: Shinozuka Y Kojima S Shomura A Ichimura H Yano M Yamamoto K Sasaki T | Journal: DNA Res .
Citation: V : 6 ( 2 ) P : 123-9 Year: 1999 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub10382970 Accession (PMID): 10382970 | Matching Sentences: [ Sen. 9, subscore: 1.00 ]: Thirty-five MADS box gene homologues were identified through a large-scale cDNA analysis in rice . Based on the nucleotide sequences of the 3-untranslated region , these clones were classified into 11 independent species . Seven species were found to be new among the rice MADS box gene family , and the other 4 corresponded to the previously reported OsMADS1 , OsMADS2 , OsMADS4 , and OsMADS5 . The full nucleotide sequences of the 7 new species were determined . Each clone encoded a deduced protein of 164-267 amino acids . The K-domain of the MADS protein was conserved in all clones though with lower degree in clone S10304 . Reverse transcription PCR analysis showed that clones E31254 and E31864 were expressed mainly in panicles . Dendrogram analysis suggested that E31254 and E31864 are close to Arabidopsis AGL9 and AP1 , respectively . Restriction fragment length polymorphism ( RFLP ) linkage mapping revealed that the rice MADS box gene homologues reported here are not clustered but are located throughout the genome . The locus of E31864 on the RFLP map was closely linked to the long sterile lemma gene , g-1 . [ Sen. 10, subscore: 1.00 ]: Thirty-five MADS box gene homologues were identified through a large-scale cDNA analysis in rice . Based on the nucleotide sequences of the 3-untranslated region , these clones were classified into 11 independent species . Seven species were found to be new among the rice MADS box gene family , and the other 4 corresponded to the previously reported OsMADS1 , OsMADS2 , OsMADS4 , and OsMADS5 . The full nucleotide sequences of the 7 new species were determined . Each clone encoded a deduced protein of 164-267 amino acids . The K-domain of the MADS protein was conserved in all clones though with lower degree in clone S10304 . Reverse transcription PCR analysis showed that clones E31254 and E31864 were expressed mainly in panicles . Dendrogram analysis suggested that E31254 and E31864 are close to Arabidopsis AGL9 and AP1 , respectively . Restriction fragment length polymorphism ( RFLP ) linkage mapping revealed that the rice MADS box gene homologues reported here are not clustered but are located throughout the genome . The locus of E31864 on the RFLP map was closely linked to the long sterile lemma gene , g-1 .
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