| 285 matches found in 115 documents. Search time: 0.006 seconds. |
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Score: 1.00 |
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| Title: Expression of a gibberellin-induced leucine-rich repeat receptor-like protein kinase in deepwater rice and its interaction with kinase-associated protein phosphatase .
| | Author: van der Knaap E Song WY Ruan DL Sauter M Ronald PC Kende H | | Journal: Plant Physiol .
Citation: V : 120 ( 2 ) P : 559-70 | | Literature: oryza Field: abstract Doc ID: pub10364408 Accession (PMID): 10364408 | Matching Sentences:
[ Sen. 9, subscore: 1.00 ]: A cDNA encoding a rice ortholog of a kinase-associated type 2C protein phosphatase ( OsKAPP ) was cloned . KAPPs are putative downstream components in kinase-mediated signal transduction pathways . The kinase interaction domain of OsKAPP was phosphorylated in vitro by the kinase domain of OsTMK . RNA gel-blot analysis indicated that the expression of OsTMK and OsKAPP was similar in different it issues of the rice plant . In protein-binding assays , OsKAPP interacted with a receptor-like protein kinase , RLK5 of Arabidopsis , but not with the protein kinase domains of the rice and maize receptor-like protein kinases Xa21 and ZmPK1 , respectively .
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Score: 7.00 |
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| Title: Short communication : developmental control of Xa21-mediated disease resistance in rice .
| | Author: Century KS Lagman RA Adkisson M Morlan J Tobias R Schwartz K Smith A Love J Ronald PC Whalen MC .
| | Journal: Plant J Citation: V : 20 ( 2 ) P : 231-6 | | Literature: oryza Field: abstract Doc ID: pub10571882 Accession (PMID): 10571882 | Matching Sentences:
[ Sen. 6, subscore: 3.00 ]: The molecular genetic mechanism controlling the integration of the Xa21-mediated disease resistance response with the developmental program in rice is under study in this model system . Reproducible means of infecting plants at certain developmental stages were designed based on the timing of full expansion of the leaf . Xa21-resistance progressively increases from the susceptible juvenile leaf 2 stage through later stages , with 100% resistance at the adult leaf 9/10 stage . We found that Xa21 expression is independent of plant developmental stage , infection with Xoo , or wounding . Expression of the Xa21 gene transcript is not correlated with expression of Xa21 disease resistance indicating that the developmental regulation of Xa21-resistance is either controlled post-transcriptionally or by other factors .
[ Sen. 1, subscore: 1.00 ]: The rice resistance gene Xa21 confers resistance against the bacterial pathogen Xanthomonas oryzae pv . oryzae ( Xoo ) . The molecular genetic mechanism controlling the integration of the Xa21-mediated disease resistance response with the developmental program in rice is under study in this model system . Reproducible means of infecting plants at certain developmental stages were designed based on the timing of full expansion of the leaf . Xa21-resistance progressively increases from the susceptible juvenile leaf 2 stage through later stages , with 100% resistance at the adult leaf 9/10 stage . We found that Xa21 expression is independent of plant developmental stage , infection with Xoo , or wounding .
[ Sen. 2, subscore: 1.00 ]: The rice resistance gene Xa21 confers resistance against the bacterial pathogen Xanthomonas oryzae pv . oryzae ( Xoo ) . The molecular genetic mechanism controlling the integration of the Xa21-mediated disease resistance response with the developmental program in rice is under study in this model system . Reproducible means of infecting plants at certain developmental stages were designed based on the timing of full expansion of the leaf . Xa21-resistance progressively increases from the susceptible juvenile leaf 2 stage through later stages , with 100% resistance at the adult leaf 9/10 stage . We found that Xa21 expression is independent of plant developmental stage , infection with Xoo , or wounding . Expression of the Xa21 gene transcript is not correlated with expression of Xa21 disease resistance indicating that the developmental regulation of Xa21-resistance is either controlled post-transcriptionally or by other factors .
[ Sen. 4, subscore: 1.00 ]: The rice resistance gene Xa21 confers resistance against the bacterial pathogen Xanthomonas oryzae pv . oryzae ( Xoo ) . The molecular genetic mechanism controlling the integration of the Xa21-mediated disease resistance response with the developmental program in rice is under study in this model system . Reproducible means of infecting plants at certain developmental stages were designed based on the timing of full expansion of the leaf . Xa21-resistance progressively increases from the susceptible juvenile leaf 2 stage through later stages , with 100% resistance at the adult leaf 9/10 stage . We found that Xa21 expression is independent of plant developmental stage , infection with Xoo , or wounding . Expression of the Xa21 gene transcript is not correlated with expression of Xa21 disease resistance indicating that the developmental regulation of Xa21-resistance is either controlled post-transcriptionally or by other factors .
[ Sen. 5, subscore: 1.00 ]: The rice resistance gene Xa21 confers resistance against the bacterial pathogen Xanthomonas oryzae pv . oryzae ( Xoo ) . The molecular genetic mechanism controlling the integration of the Xa21-mediated disease resistance response with the developmental program in rice is under study in this model system . Reproducible means of infecting plants at certain developmental stages were designed based on the timing of full expansion of the leaf . Xa21-resistance progressively increases from the susceptible juvenile leaf 2 stage through later stages , with 100% resistance at the adult leaf 9/10 stage . We found that Xa21 expression is independent of plant developmental stage , infection with Xoo , or wounding . Expression of the Xa21 gene transcript is not correlated with expression of Xa21 disease resistance indicating that the developmental regulation of Xa21-resistance is either controlled post-transcriptionally or by other factors .
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Score: 1.00 |
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| Title: Perception of brassinosteroids by the extracellular domain of the receptor kinase BRI1 .
| | Author: He Z Wang ZY Li J Zhu Q Lamb C Ronald P Chory J | | Journal: Science Citation: V : 288 ( 5475 ) P : 2360-3 | | Literature: oryza Field: abstract Doc ID: pub10875920 Accession (PMID): 10875920 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: An assay was developed to study plant receptor kinase activation and signaling mechanisms . The extracellular leucine-rich repeat ( LRR ) and transmembrane domains of the Arabidopsis receptor kinase BRI1 , which is implicated in brassinosteroid signaling , were fused to the serine/threonine kinase domain of XA21 , the rice disease resistance receptor . The chimeric receptor initiates plant defense responses in rice cells upon treatment with brassinosteroids . These results , which indicate that the extracellular domain of BRI1 perceives brassinosteroids , suggest a general signaling mechanism for the LRR receptor kinases of plants . This system should allow the discovery of ligands for the LRR kinases , the largest group of plant receptor kinases .
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Score: 3.00 |
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| Title: [ Introduction of wide spectrum rice bacterial blight resistance gene Xa21 into two-line genic male sterile rice variety Peiai 64S ] | | Author: Zhao B Wang WM Zheng XW Wang CL Ma BJ Xue QZ Zhu LH Zhai WX .
| | Journal: Sheng Wu Gong Cheng Xue Bao Citation: V : 16 ( 2 ) P : 137-41 | | Literature: oryza Field: abstract Doc ID: pub10976312 Accession (PMID): 10976312 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Agrobacterium-mediated transformation of two-line genic male sterile Indica rice variety Peiai 64S was conducted using a cloned gene , Xa21 , as the foreign gene and mature embryo calli as the recipients . A total of 46 transgenic plants had been obtained . The PCR analysis and Southern blotting showed the integration of Xa21 gene into the genome the transgenic plants . Results of inoculation with philippine race 6 of Xanthomonas oryzae pv . oryzae indicated that most of transgenic plants obtained high resistance to rice bacterial blight disease ( Xoo ) . Analyses of T1 plants of the tested transgenic lines showed that integrated Xa21 gene could be steadily inherited and segregated in a 3 : 1 ratio .
[ Sen. 3, subscore: 1.00 ]: Agrobacterium-mediated transformation of two-line genic male sterile Indica rice variety Peiai 64S was conducted using a cloned gene , Xa21 , as the foreign gene and mature embryo calli as the recipients . A total of 46 transgenic plants had been obtained . The PCR analysis and Southern blotting showed the integration of Xa21 gene into the genome the transgenic plants . Results of inoculation with philippine race 6 of Xanthomonas oryzae pv . oryzae indicated that most of transgenic plants obtained high resistance to rice bacterial blight disease ( Xoo ) . Analyses of T1 plants of the tested transgenic lines showed that integrated Xa21 gene could be steadily inherited and segregated in a 3 : 1 ratio .
[ Sen. 5, subscore: 1.00 ]: Agrobacterium-mediated transformation of two-line genic male sterile Indica rice variety Peiai 64S was conducted using a cloned gene , Xa21 , as the foreign gene and mature embryo calli as the recipients . A total of 46 transgenic plants had been obtained . The PCR analysis and Southern blotting showed the integration of Xa21 gene into the genome the transgenic plants . Results of inoculation with philippine race 6 of Xanthomonas oryzae pv . oryzae indicated that most of transgenic plants obtained high resistance to rice bacterial blight disease ( Xoo ) . Analyses of T1 plants of the tested transgenic lines showed that integrated Xa21 gene could be steadily inherited and segregated in a 3 : 1 ratio .
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Score: 1.00 |
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| Title: The rice Rim2 transcript accumulates in response to Magnaporthe grisea and its predicted protein product shares similarity with TNP2-like proteins encoded by CACTA transposons .
| | Author: He ZH Dong HT Dong JX Li DB Ronald PC .
| | Journal: Mol . Gen . Genet . Citation: V : 264 ( 1-2 ) P : 2-10 | | Literature: oryza Field: abstract Doc ID: pub11016827 Accession (PMID): 11016827 | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: A rice transcript , Rim2 , was identified that accumulated in both incompatible and compatible interactions between rice and Magnaporthe grisea . The Rim2 transcript also accumulated in response to treatment with a cell wall elicitor derived from M grisea . A 3 . 3-kb RIM2 cDNA clone was isolated and is predicted to encode a protein of 653 amino acids , which shares 32 55% identity with TNP2-like proteins encoded by CACTA transposons of other plants . A 1 . 05-kb segment of the Rim2 sequence shows 82% nucleotide sequence identity with sequences flanking the A1 and C members of the rice Xa21 disease resistance gene family . The 5-upstream region of Rim2 was cloned and the transcriptional start sites were identified . The 5 and 3 noncoding termini of Rim2 are AT-rich . A cis-element showing similarity to a sequence that mediates defense-associated transcriptional activation of the tobacco retrotransposon Tnt1 , and four motifs that fit the consensus sequence of the elicitor-responsive elements in the promoters of the parsley PR-1 genes were found in the 5-upstream region . Four imperfect tandem repeats were identified in the 3 noncoding terminus .
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Score: 2.00 |
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| Title: [ Amplification and analysis of T-DNA flanking sequences in transgenic rice ] | | Author: Fang J Zhai WX Wang WM Li SW Zhu LH .
| | Journal: Yi Chuan Xue Bao Citation: V : 28 ( 4 ) P : 345-51 | | Literature: oryza Field: abstract Doc ID: pub11329876 Accession (PMID): 11329876 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Rice transformation mediated by Agrobacterium tumefaciens has technically matured to some extent , but the mechanics of T-DNA integration in transgenic rice remains largely unknown . Using thermal asymmetric interlaced PCR ( TAIL-PCR ) , we analyzed the flanking sequences of T-DNAs in transgenic rice plants , in which the resistance gene for rice bacterial blight disease , Xa21 , had been integrated stably . Sequence analysis of 24 fragments amplified by TAIL-PCR showed that of them 14 were rice genomic DNA , 9 contained vector backbone sequences , and one was a fragment of the exogenous gene Xa21 . The characteristics of the 14 rice genomic DNA sequences at T-DNA integrated sites are significantly different from those of the reported rice genomic sequences into which exogenous genes were integrated through direct DNA transformation methods . T-DNA border sequences integrated in rice genome have similar features as those integrated in dicotyledonous genomes . In the backbone containing flanking sequences ( 37 . 5% , 9/24 ) vector backbones appeared in different types .
[ Sen. 3, subscore: 1.00 ]: Rice transformation mediated by Agrobacterium tumefaciens has technically matured to some extent , but the mechanics of T-DNA integration in transgenic rice remains largely unknown . Using thermal asymmetric interlaced PCR ( TAIL-PCR ) , we analyzed the flanking sequences of T-DNAs in transgenic rice plants , in which the resistance gene for rice bacterial blight disease , Xa21 , had been integrated stably . Sequence analysis of 24 fragments amplified by TAIL-PCR showed that of them 14 were rice genomic DNA , 9 contained vector backbone sequences , and one was a fragment of the exogenous gene Xa21 . The characteristics of the 14 rice genomic DNA sequences at T-DNA integrated sites are significantly different from those of the reported rice genomic sequences into which exogenous genes were integrated through direct DNA transformation methods . T-DNA border sequences integrated in rice genome have similar features as those integrated in dicotyledonous genomes . In the backbone containing flanking sequences ( 37 . 5% , 9/24 ) vector backbones appeared in different types .
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Score: 1.00 |
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| Title: Construction of a 1 . 2-Mb contig including the citrus tristeza virus resistance gene locus using a bacterial artificial chromosome library of Poncirus trifoliata ( L ) Raf .
| | Author: Yang ZN Ye XR Choi S Molina J Moonan F Wing RA Roose ML Mirkov TE .
| | Journal: Genome Citation: V : 44 ( 3 ) P : 382-93 | | Literature: oryza Field: abstract Doc ID: pub11444697 Accession (PMID): 11444697 | Matching Sentences:
[ Sen. 9, subscore: 1.00 ]: The chromosome walk across the Ctv locus was initiated using three closely linked genetic markers : C19 , AD8 , and Z16 . The walk has been completed and a contig of ca 1 . 2 Mb was constructed . Based on new data , the genetic map in the Ctv region was revised , with Ctv being located between AD8-Z16 and C19 at distances of 1 . 2 and 0 . 6 cM , respectively . Utilizing DNA fragments isolated from the contig as RFLP markers , the Ctv locus was further mapped to a region of ca 300 kb . This contig contains several putative disease-resistance genes similar to the rice Xa21 gene , the tomato Cf-2 gene , and the Arabidopsis thaliana RPS2 gene . This library will therefore allow cloning of Ctv and other putative disease-resistance genes .
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Score: 2.00 |
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| Title: Marker assisted selection of bacterial blight resistance genes in rice .
| | Author: Davierwala AP Reddy AP Lagu MD Ranjekar PK Gupta VS .
| | Journal: Biochem . Genet . Citation: V : 39 ( 7-8 ) P : 261-78 | | Literature: oryza Field: abstract Doc ID: pub11590832 Accession (PMID): 11590832 | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: Bacterial leaf blight caused by Xanthomonas oryzae pv . oryzae is one of the most important diseases affecting rice production in Asia . We were interested in surveying rice genotypes that are popularly used in the Indian breeding program for conferring resistance to bacterial blight , using 11 STMS and 6 STS markers . The basis of selection of these DNA markers was their close linkage to xa5 , xa13 , and Xa21 genes and their positions on the rice genetic map relative to bacterial blight resistance genes . Eight lines were found to contain the xa5 gene while two lines contained Xa21 gene and none of the lines contained the xa13 gene with the exception of its near-isogenic line . Using the polymorphic markers obtained in the initial survey , marker-assisted selection was performed in the F3 population of a cross between IR-64 and IET-14444 to detect lines containing multiple resistance genes . Of the 59 progeny lines analyzed , eight lines contained both the resistance genes , xa5 and Xa4 .
[ Sen. 4, subscore: 1.00 ]: Bacterial leaf blight caused by Xanthomonas oryzae pv . oryzae is one of the most important diseases affecting rice production in Asia . We were interested in surveying rice genotypes that are popularly used in the Indian breeding program for conferring resistance to bacterial blight , using 11 STMS and 6 STS markers . The basis of selection of these DNA markers was their close linkage to xa5 , xa13 , and Xa21 genes and their positions on the rice genetic map relative to bacterial blight resistance genes . Eight lines were found to contain the xa5 gene while two lines contained Xa21 gene and none of the lines contained the xa13 gene with the exception of its near-isogenic line . Using the polymorphic markers obtained in the initial survey , marker-assisted selection was performed in the F3 population of a cross between IR-64 and IET-14444 to detect lines containing multiple resistance genes . Of the 59 progeny lines analyzed , eight lines contained both the resistance genes , xa5 and Xa4 .
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Score: 3.00 |
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| Title: Are the dominant and recessive plant disease resistance genes similar?
A case study of rice R genes and Xanthomonas oryzae pv . oryzae races .
| | Author: Li ZK Sanchez A Angeles E Singh S Domingo J Huang N Khush GS .
| | Journal: Genetics Citation: V : 159 ( 2 ) P : 757-65 | | Literature: oryza Field: abstract Doc ID: pub11606550 Accession (PMID): 11606550 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The resistance of rice to its bacterial blight pathogen Xanthomonas oryzae pv . oryzae ( Xoo ) has both qualitative and quantitative components that were investigated using three near-isogenic line sets for four resistance ( R ) genes ( Xa4 , xa5 , xa13 , and Xa21 ) and 12 Xoo races . Our results indicate that these two resistance components of rice plants were associated with the properties of the R genes . The qualitative component of the R genes was reflected by their large effects against corresponding avirulent Xoo races . The quantitative component of the R genes was their residual effects against corresponding virulent races and their epistatic effects , which together could lead to high-level resistance in a race-specific manner . Our results revealed important differences between the different types of R genes .
[ Sen. 6, subscore: 1.00 ]: Our results indicate that these two resistance components of rice plants were associated with the properties of the R genes . The qualitative component of the R genes was reflected by their large effects against corresponding avirulent Xoo races . The quantitative component of the R genes was their residual effects against corresponding virulent races and their epistatic effects , which together could lead to high-level resistance in a race-specific manner . Our results revealed important differences between the different types of R genes . Two R genes , Xa4 and Xa21 , showed complete dominance against the avirulent Xoo races and had large residual effects against virulent ones . They acted independently and cumulatively , suggesting they are involved in different pathways of the rice defensive system . The third R gene , xa5 , showed partial dominance or additivity to the avirulent Xoo races and had relatively small but significant residual effects against the virulent races . In contrast , xa13 was completely recessive , had no residual effects against the virulent races , and showed more pronounced race specificity . There was a strong interaction leading to increased resistance between xa13 and xa5 and between either of them and Xa4 or Xa21 , suggesting their regulatory roles in the rice defensive pathway ( s ) .
[ Sen. 10, subscore: 1.00 ]: Two R genes , Xa4 and Xa21 , showed complete dominance against the avirulent Xoo races and had large residual effects against virulent ones . They acted independently and cumulatively , suggesting they are involved in different pathways of the rice defensive system . The third R gene , xa5 , showed partial dominance or additivity to the avirulent Xoo races and had relatively small but significant residual effects against the virulent races . In contrast , xa13 was completely recessive , had no residual effects against the virulent races , and showed more pronounced race specificity . There was a strong interaction leading to increased resistance between xa13 and xa5 and between either of them and Xa4 or Xa21 , suggesting their regulatory roles in the rice defensive pathway ( s ) . Our results indicated that high-level and durable resistance to Xoo should be more efficiently achieved by pyramiding different types of R genes .
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Score: 3.00 |
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| Title: [ A genetically modified japonica restorer line , C418-Xa21 , and its hybrid rice with bacterial blight resistance ] | | Author: Li XB Yi CD Zhai WX Yang ZY Zhu LH .
| | Journal: Sheng Wu Gong Cheng Xue Bao Citation: V : 17 ( 4 ) P : 380-4 | | Literature: oryza Field: abstract Doc ID: pub11702691 Accession (PMID): 11702691 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The cloned bacterial blight ( BB ) resistance gene Xa21 was transferred into C418 , a major restorer line of japonica hybrid rice in China , using an Agrobacterium-mediated system . The integrated single copy of transgene displayed a 3 : 1 segregation ratio in T1 generation in PCR and resistance analyses . The transgenic homozygous C418-Xa21 lines were selected in T2 generation through PCR and resistance analyses . The selected transgenic restorer lines were then crossed with a commonly used sterile line , TijinA , to produce Xa21 transgenic hybrid rice . Molecular analysis revealed that the produced hybrid rice , named as Tiyou418-Xa21 , inherited the transgene .
[ Sen. 4, subscore: 1.00 ]: The cloned bacterial blight ( BB ) resistance gene Xa21 was transferred into C418 , a major restorer line of japonica hybrid rice in China , using an Agrobacterium-mediated system . The integrated single copy of transgene displayed a 3 : 1 segregation ratio in T1 generation in PCR and resistance analyses . The transgenic homozygous C418-Xa21 lines were selected in T2 generation through PCR and resistance analyses . The selected transgenic restorer lines were then crossed with a commonly used sterile line , TijinA , to produce Xa21 transgenic hybrid rice . Molecular analysis revealed that the produced hybrid rice , named as Tiyou418-Xa21 , inherited the transgene . Both C418-Xa21 and Tiyou418-Xa21 plants displayed high resistance with a broad spectrum to Xoo races and maintained their normal elite agronomic characters . We also observed that the resistance level of Tiyou418-Xa21 was obviously higher than that of C418-Xa21 which may be attributed to their differences in genetic background . The propagation of this BB resistant hybrid variety with the transgene Xa21 with extend hybrid rice production in north China .
[ Sen. 8, subscore: 1.00 ]: The selected transgenic restorer lines were then crossed with a commonly used sterile line , TijinA , to produce Xa21 transgenic hybrid rice . Molecular analysis revealed that the produced hybrid rice , named as Tiyou418-Xa21 , inherited the transgene . Both C418-Xa21 and Tiyou418-Xa21 plants displayed high resistance with a broad spectrum to Xoo races and maintained their normal elite agronomic characters . We also observed that the resistance level of Tiyou418-Xa21 was obviously higher than that of C418-Xa21 which may be attributed to their differences in genetic background . The propagation of this BB resistant hybrid variety with the transgene Xa21 with extend hybrid rice production in north China .
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