| 63 matches found in 28 documents. Search time: 0.002 seconds. |
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Score: 1.00 |
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| | Author: Orbach MJ Farrall L Sweigard JA Chumley FG Valent B | | Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11090206 Accession (PMID): 11090206 | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: Analysis of spontaneous avr-pita ( - ) mutants indicated that the gene is located in a telomeric 6 . 5-kb BglII restriction fragment .
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Score: 3.00 |
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| | Author: Opassiri R Hua Y Wara-Aswapati O Akiyama T Svasti J Esen A Ketudat Cairns JR .
| | Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub14692878 Accession (PMID): 14692878 | Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: The bglu1 cDNA for a beta-glucosidase cloned from rice ( Oryza sativa L ) seedlings was expressed as a soluble and active protein in Escherichia coli and designated BGlu1 .
[ Sen. 6, subscore: 1.00 ]: BGlu1 also had high transglucosylation activity towards pyridoxine , producing pyridoxine 5-O-beta-D-glucopyranoside in the presence of the glucose donor p-nitrophenyl beta-D-glucoside .
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Score: 4.00 |
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| | Author: Chuenchor W Pengthaisong S Yuvaniyama J Opassiri R Svasti J Ketudat Cairns JR .
| | Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16880561 Accession (PMID): 16880561 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Rice ( Oryza sativa ) BGlu1 beta-glucosidase was expressed in Escherichia coli with N-terminal thioredoxin and hexahistidine tags and purified by immobilized metal-affinity chromatography ( IMAC ) .
[ Sen. 2, subscore: 1.00 ]: After removal of the N-terminal tags , cation-exchange and S-200 gel-filtration chromatography yielded a 50 kDa BGlu1 with >95% purity .
[ Sen. 4, subscore: 1.00 ]: Small tetragonal crystals of BGlu1 with and without inhibitor grew in 18% ( w/v ) PEG 8000 with 0 . 1 M sodium cacodylate pH 6 . 5 and 0 . 2 M zinc acetate .
[ Sen. 5, subscore: 1.00 ]: Crystals of BGlu1 with inhibitor were streak-seeded into 23% ( w/v ) PEG MME 5000 , 0 . 2 M ammonium sulfate , 0 . 1 M MES pH 6 . 7 to yield larger crystals .
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Score: 4.00 |
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| | Author: Hommalai G Withers SG Chuenchor W Cairns JR Svasti J | | Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub17405771 Accession (PMID): 17405771 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Rice ( BGlu1 ) beta-glucosidase is a glycosyl hydrolase family 1 enzyme that acts as an exoglucanase on beta- ( 1 , 4 ) - and short beta- ( 1 , 3 ) -linked gluco-oligosaccharides .
[ Sen. 2, subscore: 1.00 ]: Mutations of BGlu1 beta-glucosidase at glutamate residue 414 of its natural precursor destroyed the enzymes catalytic activity , but the enzyme could be rescued in the presence of the anionic nucleophiles formate and azide , which verifies that this residue is the catalytic nucleophile .
[ Sen. 8, subscore: 1.00 ]: The BGlu1 E414G glycosynthase was found to prefer longer chain length oligosaccharides that occupy at least three sugar residue binding subsites as acceptors for productive transglucosylation .
[ Sen. 9, subscore: 1.00 ]: This is the first report of a beta-glucansynthase derived from an exoglycosidase that can produce long chain cello-oligosaccharides , which likely reflects the extended oligosaccharide binding site of rice BGlu1 beta-glucosidase .
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Score: 3.00 |
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| | Author: Chuenchor W Pengthaisong S Robinson RC Yuvaniyama J Oonanant W Bevan DR Esen A Chen CJ Opassiri R Svasti J Cairns JR | | Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub18308333 Accession (PMID): 18308333 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The structures of rice BGlu1 beta-glucosidase , a plant beta-glucosidase active in hydrolyzing cell wall-derived oligosaccharides , and its covalent intermediate with 2-deoxy-2-fluoroglucoside have been solved at 2 . 2 A and 1 . 55 A resolution , respectively .
[ Sen. 5, subscore: 1.00 ]: The coordination of the hydroxyl groups suggests that sugars are positioned as acceptors for transglycosylation by their interactions with E176 , the catalytic acid/base , and Y131 , which is conserved in barley BGQ60/beta-II beta-glucosidase , that has oligosaccharide hydrolysis and transglycosylation activity similar to rice BGlu1 .
[ Sen. 6, subscore: 1.00 ]: As the rice and barley enzymes have different preferences for cellobiose and cellotriose , residues that appeared to interact with docked oligosaccharides were mutated to those of the barley enzyme to see if the relative activities of rice BGlu1 toward these substrates could be changed to those of BGQ60 .
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Score: 1.00 |
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| | Author: Harnpicharnchai P Champreda V Sornlake W Eurwilaichitr L | | Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub18602476 Accession (PMID): 18602476 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: A fungal strain , BCC2871 ( Periconia sp . ) , was found to produce a thermotolerant beta-glucosidase , BGL I , with high potential for application in biomass conversion .
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Score: 1.00 |
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| | Author: Liu Y Xuan S Long C Long M Hu Z | | Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub18808002 Accession (PMID): 18808002 | Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: The maximal beta-1 , 4-glucosidase ( BGL ) activity was 1662 u/mL which is 1 . 49 times of the previous and the maximal exo-beta-1 , 4-glucanases ( CBH ) activity was 2770 u/mL which is 1 . 36 times of the previous , cultured in the optimal condition for three days .
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Score: 1.00 |
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| | Author: Jeya M Zhang YW Kim IW Lee JK | | Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19540109 Accession (PMID): 19540109 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: The strain showed maximum endoglucanase ( EG ) , cellobiohydrolase ( CBH ) and beta-glucosidase ( BGL ) activities of 55 , 0 . 28 and 5 . 0 U/mg-protein , respectively .
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Score: 1.00 |
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| | Author: Seshadri S Akiyama T Opassiri R Kuaprasert B Cairns JK | | Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19587102 Accession (PMID): 19587102 | Matching Sentences:
[ Sen. 7, subscore: 1.00 ]: The position of the covalently trapped 2-F-glucosyl residue in the enzyme was similar to that in a 2-F-glucosyl intermediate complex of Os3BGlu7 ( rice BGlu1 ) .
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Score: 10.00 |
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| | Author: Jeya M Joo AR Lee KM Tiwari MK Lee KM Kim SH Lee JK | | Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20043150 Accession (PMID): 20043150 | Matching Sentences:
[ Sen. 4, subscore: 3.00 ]: An extracellular BGL was purified to homogeneity by sequential chromatography of P purpurogenum culture supernatants , and the purified BGL showed higher activity ( V ( max ) = 934 U mg protein ( -1 ) ) than most BGLs from other sources .
[ Sen. 2, subscore: 2.00 ]: When rice straw and corn steep powder were used as carbon and nitrogen sources , respectively , the maximal BGL activity of 12 . 3 U ml ( -1 ) , one of the highest levels among BGL-producing microorganisms was observed .
[ Sen. 1, subscore: 1.00 ]: A novel beta-glucosidase ( BGL ) -producing strain was isolated and identified as Penicillium purpurogenum KJS506 based on its morphology and internal transcribed spacer ( ITS ) rDNA gene sequence .
[ Sen. 3, subscore: 1.00 ]: The optimum temperature and pH for BGL production were 32 degrees C and 4 , respectively .
[ Sen. 5, subscore: 1.00 ]: The complete ORF of bgl3 was cloned from P purpurogenum by a modified thermal asymmetric interlaced polymerase chain reaction .
[ Sen. 6, subscore: 1.00 ]: The bgl3 gene consists of a 2 , 571-bp ORF and encodes a putative protein containing 856 amino acids with a calculated molecular mass of 89 , 624 Da .
[ Sen. 8, subscore: 1.00 ]: The present results should contribute to improved industrial production of BGL by P purpurogenum KJS506 .
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