45 matches found in 19 documents. Search time: 0.009 seconds. |
|
Score: 1.00 | Title: Quantitative trait loci associated with leaf and neck blast resistance in recombinant inbred line population of rice ( Oryza sativa ) .
| Journal: DNA Res .
Year: 2002 | Literature: oryza Field: abstract Doc ID: pub12168952 Accession (PMID): 12168952 | Matching Sentences: [ Sen. 8, subscore: 1.00 ]: In particular , the QTL ( ch9 ) was mapped near the Pi5 ( t ) locus .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 7.00 | Title: Genetic and physical mapping of Pi5 ( t ) , a locus associated with broad-spectrum resistance to rice blast | Journal: Mol . Genet . Genomics Year: 2003 | Literature: oryza Field: abstract Doc ID: pub12756540 Accession (PMID): 12756540 | Matching Sentences: [ Sen. 9, subscore: 2.00 ]: DNA gel-blot analyses indicated that the region around Pi3 ( t ) is identical to that of Pi5 ( t ) , suggesting that Pi3 ( t ) and Pi5 ( t ) are the same resistance gene . [ Sen. 1, subscore: 1.00 ]: To gain an understanding of the molecular basis for resistance to rice blast ( Magnaporthe grisea ) , we have initiated a project to clone Pi5 ( t ) , a locus associated with broad-spectrum resistance to diverse blast isolates . [ Sen. 2, subscore: 1.00 ]: AFLP-derived markers linked to Pi5 ( t ) -mediated resistance were isolated using bulked segregant analysis of F ( 2 ) populations generated by crossing three recombinant inbred lines ( RILs ) , RIL125 , RIL249 , and RIL260 with the susceptible line CO39 . [ Sen. 6, subscore: 1.00 ]: Using these resources Pi5 ( t ) was mapped to a 170-kb interval , and a contiguous set of BIBAC clones spanning this region was constructed . [ Sen. 7, subscore: 1.00 ]: It had previously been suggested that Pi3 ( t ) and Pi5 ( t ) might be allelic , due to their identical resistance spectrum and tight linkage . [ Sen. 8, subscore: 1.00 ]: We therefore compared genomic regions for lines containing Pi3 ( t ) using the Pi5 ( t ) -linked markers .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 6.00 | Title: Use of Pi5 ( t ) markers in marker-assisted selection to screen for cultivars with resistance to Magnaporthe grisea .
| Journal: Theor . Appl . Genet . Year: 2004 | Literature: oryza Field: abstract Doc ID: pub15141293 Accession (PMID): 15141293 | Matching Sentences: [ Sen. 2, subscore: 1.00 ]: The rice Pi5 ( t ) locus confers broad-spectrum resistance to Magnaporthe grisea , the causal agent of rice blast disease . [ Sen. 3, subscore: 1.00 ]: It has been hypothesized that the Pi5 ( t ) locus carries the same gene as that encoded by the Pi3 ( t ) and Pii ( t ) loci . [ Sen. 4, subscore: 1.00 ]: We developed three PCR-based dominant markers ( JJ80-T3 , JJ81-T3 , and JJ113-T3 ) from three previously identified BIBAC clones-JJ80 , JJ81 , and JJ113-that are linked to the Pi5 ( t ) locus . [ Sen. 5, subscore: 1.00 ]: PCR analysis of 24 monogenic lines revealed that these markers are present only in lines that carry Pi5 ( t ) , Pi3 ( t ) , and Pii ( t ) . [ Sen. 6, subscore: 1.00 ]: PCR and DNA gel-blot analysis of candidate resistance lines using JJ80-T3 , JJ81-T3 , and JJ113-T3 indicated that Tetep is the likely donor of Pi5 ( t ) . [ Sen. 9, subscore: 1.00 ]: The genomic structure of three of these resistant varieties ( ie , IR72 , Taebaeg , Jahyangdo ) is most similar to that of Pi5 ( t ) .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 | Title: The highly charged region of plant beta-type phosphatidylinositol 4-kinase is involved in membrane targeting and phospholipid binding .
| Journal: Plant Mol . Biol . Year: 2006 | Literature: oryza Field: abstract Doc ID: pub16649109 Accession (PMID): 16649109 | Matching Sentences: [ Sen. 9, subscore: 1.00 ]: Recombinant PPC peptide , expressed in E coli , strongly binds phosphatidic acid , PI and PI4P , but not phosphatidylcholine , PI5P , or PI ( 4 , 5 ) P2 in vitro , providing insights into potential mechanisms for regulating sub-cellular localization and lipid binding for the plant beta-type PI4Ks .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 11.00 | Title: Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes .
| Journal: Genetics Year: 2009 | Literature: oryza Field: abstract Doc ID: pub19153255 Accession (PMID): 19153255 | Matching Sentences: [ Sen. 3, subscore: 4.00 ]: Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50 , a susceptible rice cultivar , and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval Sequence analysis of this genomic region identified two candidate genes , Pi5-1 and Pi5-2 , which encode proteins carrying three motifs characteristic of R genes : an N-terminal coiled-coil ( CC ) motif , a nucleotide-binding ( NB ) domain , and a leucine-rich repeat ( LRR ) motif . [ Sen. 4, subscore: 2.00 ]: In genetic transformation experiments of a susceptible rice cultivar , neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to M oryzae . [ Sen. 6, subscore: 2.00 ]: Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge , whereas the Pi5-2 gene is constitutively expressed . [ Sen. 2, subscore: 1.00 ]: To understand the molecular basis of Pi5-mediated resistance to M oryzae , we cloned the resistance ( R ) gene at this locus using a map-based cloning strategy . [ Sen. 5, subscore: 1.00 ]: In contrast , transgenic rice plants expressing both of these genes , generated by crossing transgenic lines carrying each gene individually , conferred Pi5-mediated resistance to M oryzae . [ Sen. 7, subscore: 1.00 ]: These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to M oryzae .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |