| 285 matches found in 115 documents. Search time: 0.005 seconds. |
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| Title: Expression of a gibberellin-induced leucine-rich repeat receptor-like protein kinase in deepwater rice and its interaction with kinase-associated protein phosphatase .
| | Journal: Plant Physiol .
| | Literature: oryza Field: abstract Doc ID: pub10364408 | Matching Sentences:
[ Sen. 9, subscore: 1.00 ]: In protein-binding assays , OsKAPP interacted with a receptor-like protein kinase , RLK5 of Arabidopsis , but not with the protein kinase domains of the rice and maize receptor-like protein kinases Xa21 and ZmPK1 , respectively .
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Score: 7.00 |
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| Title: Short communication : developmental control of Xa21-mediated disease resistance in rice .
| | Journal: Plant J | | Literature: oryza Field: abstract Doc ID: pub10571882 | Matching Sentences:
[ Sen. 6, subscore: 3.00 ]: Expression of the Xa21 gene transcript is not correlated with expression of Xa21 disease resistance indicating that the developmental regulation of Xa21-resistance is either controlled post-transcriptionally or by other factors .
[ Sen. 1, subscore: 1.00 ]: The rice resistance gene Xa21 confers resistance against the bacterial pathogen Xanthomonas oryzae pv . oryzae ( Xoo ) .
[ Sen. 2, subscore: 1.00 ]: The molecular genetic mechanism controlling the integration of the Xa21-mediated disease resistance response with the developmental program in rice is under study in this model system .
[ Sen. 4, subscore: 1.00 ]: Xa21-resistance progressively increases from the susceptible juvenile leaf 2 stage through later stages , with 100% resistance at the adult leaf 9/10 stage .
[ Sen. 5, subscore: 1.00 ]: We found that Xa21 expression is independent of plant developmental stage , infection with Xoo , or wounding .
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| Title: Perception of brassinosteroids by the extracellular domain of the receptor kinase BRI1 .
| | Journal: Science | | Literature: oryza Field: abstract Doc ID: pub10875920 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: The extracellular leucine-rich repeat ( LRR ) and transmembrane domains of the Arabidopsis receptor kinase BRI1 , which is implicated in brassinosteroid signaling , were fused to the serine/threonine kinase domain of XA21 , the rice disease resistance receptor .
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Score: 3.00 |
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| Title: [ Introduction of wide spectrum rice bacterial blight resistance gene Xa21 into two-line genic male sterile rice variety Peiai 64S ] | | Journal: Sheng Wu Gong Cheng Xue Bao | | Literature: oryza Field: abstract Doc ID: pub10976312 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Agrobacterium-mediated transformation of two-line genic male sterile Indica rice variety Peiai 64S was conducted using a cloned gene , Xa21 , as the foreign gene and mature embryo calli as the recipients .
[ Sen. 3, subscore: 1.00 ]: The PCR analysis and Southern blotting showed the integration of Xa21 gene into the genome the transgenic plants .
[ Sen. 5, subscore: 1.00 ]: Analyses of T1 plants of the tested transgenic lines showed that integrated Xa21 gene could be steadily inherited and segregated in a 3 : 1 ratio .
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Score: 1.00 |
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| Title: The rice Rim2 transcript accumulates in response to Magnaporthe grisea and its predicted protein product shares similarity with TNP2-like proteins encoded by CACTA transposons .
| | Journal: Mol . Gen . Genet . | | Literature: oryza Field: abstract Doc ID: pub11016827 | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: A 1 . 05-kb segment of the Rim2 sequence shows 82% nucleotide sequence identity with sequences flanking the A1 and C members of the rice Xa21 disease resistance gene family .
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Score: 2.00 |
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| Title: [ Amplification and analysis of T-DNA flanking sequences in transgenic rice ] | | Journal: Yi Chuan Xue Bao | | Literature: oryza Field: abstract Doc ID: pub11329876 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Using thermal asymmetric interlaced PCR ( TAIL-PCR ) , we analyzed the flanking sequences of T-DNAs in transgenic rice plants , in which the resistance gene for rice bacterial blight disease , Xa21 , had been integrated stably .
[ Sen. 3, subscore: 1.00 ]: Sequence analysis of 24 fragments amplified by TAIL-PCR showed that of them 14 were rice genomic DNA , 9 contained vector backbone sequences , and one was a fragment of the exogenous gene Xa21 .
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| Title: Construction of a 1 . 2-Mb contig including the citrus tristeza virus resistance gene locus using a bacterial artificial chromosome library of Poncirus trifoliata ( L ) Raf .
| | Journal: Genome | | Literature: oryza Field: abstract Doc ID: pub11444697 | Matching Sentences:
[ Sen. 9, subscore: 1.00 ]: This contig contains several putative disease-resistance genes similar to the rice Xa21 gene , the tomato Cf-2 gene , and the Arabidopsis thaliana RPS2 gene .
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Score: 2.00 |
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| Title: Marker assisted selection of bacterial blight resistance genes in rice .
| | Journal: Biochem . Genet . | | Literature: oryza Field: abstract Doc ID: pub11590832 | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: The basis of selection of these DNA markers was their close linkage to xa5 , xa13 , and Xa21 genes and their positions on the rice genetic map relative to bacterial blight resistance genes .
[ Sen. 4, subscore: 1.00 ]: Eight lines were found to contain the xa5 gene while two lines contained Xa21 gene and none of the lines contained the xa13 gene with the exception of its near-isogenic line .
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Score: 3.00 |
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| Title: Are the dominant and recessive plant disease resistance genes similar?
A case study of rice R genes and Xanthomonas oryzae pv . oryzae races .
| | Journal: Genetics | | Literature: oryza Field: abstract Doc ID: pub11606550 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The resistance of rice to its bacterial blight pathogen Xanthomonas oryzae pv . oryzae ( Xoo ) has both qualitative and quantitative components that were investigated using three near-isogenic line sets for four resistance ( R ) genes ( Xa4 , xa5 , xa13 , and Xa21 ) and 12 Xoo races .
[ Sen. 6, subscore: 1.00 ]: Two R genes , Xa4 and Xa21 , showed complete dominance against the avirulent Xoo races and had large residual effects against virulent ones .
[ Sen. 10, subscore: 1.00 ]: There was a strong interaction leading to increased resistance between xa13 and xa5 and between either of them and Xa4 or Xa21 , suggesting their regulatory roles in the rice defensive pathway ( s ) .
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Score: 3.00 |
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| Title: [ A genetically modified japonica restorer line , C418-Xa21 , and its hybrid rice with bacterial blight resistance ] | | Journal: Sheng Wu Gong Cheng Xue Bao | | Literature: oryza Field: abstract Doc ID: pub11702691 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The cloned bacterial blight ( BB ) resistance gene Xa21 was transferred into C418 , a major restorer line of japonica hybrid rice in China , using an Agrobacterium-mediated system .
[ Sen. 4, subscore: 1.00 ]: The selected transgenic restorer lines were then crossed with a commonly used sterile line , TijinA , to produce Xa21 transgenic hybrid rice .
[ Sen. 8, subscore: 1.00 ]: The propagation of this BB resistant hybrid variety with the transgene Xa21 with extend hybrid rice production in north China .
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Score: 9.00 |
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| Title: Biochemical characterization of the kinase domain of the rice disease resistance receptor-like kinase XA21 .
| | Journal: J Biol . Chem . | | Literature: oryza Field: abstract Doc ID: pub11927577 | Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: Moreover , the active XA21 kinase can not phosphorylate a kinase-deficient mutant of XA21 kinase .
[ Sen. 1, subscore: 1.00 ]: The rice disease resistance gene , Xa21 , encodes a receptor kinase-like protein consisting of leucine-rich repeats in the putative extracellular domain and a serine/threonine kinase in the putative intracellular domain .
[ Sen. 2, subscore: 1.00 ]: The putative XA21 kinase domain was expressed as maltose-binding and glutathione S-transferase fusion proteins in Escherichia coli .
[ Sen. 5, subscore: 1.00 ]: The relative phosphorylation rate of the XA21 kinase against increasing enzyme concentrations follows a first-order rather than second-order kinetics , indicating an intramolecular phosphorylation mechanism .
[ Sen. 7, subscore: 1.00 ]: The enzymatic activity of the XA21 kinase in a buffer containing Mn ( 2+ ) is at least 15 times higher than that with Mg ( 2+ ) .
[ Sen. 8, subscore: 1.00 ]: The K ( m ) and V ( max ) of XA21 kinase for ATP are 0 . 3 microm and 8 . 4 nmol/mg/min , respectively .
[ Sen. 9, subscore: 1.00 ]: Tryptic phosphopeptide mapping reveals that multiple sites on the XA21 kinase are phosphorylated .
[ Sen. 10, subscore: 1.00 ]: Finally , our data suggest that the region of XA21 kinase corresponding to the RD kinase activation domain is not phosphorylated , revealing a distinct mode of action compared with the tomato Pto serine/threonine kinase conferring disease resistance .
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Score: 3.00 |
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| Title: The Xanthomonas oryzae pv . lozengeoryzae raxP and raxQ genes encode an ATP sulphurylase and adenosine-5-phosphosulphate kinase that are required for AvrXa21 avirulence activity .
| | Journal: Mol . Microbiol . | | Literature: oryza Field: abstract Doc ID: pub11967067 | Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: Xanthomonas oryzae pv . oryzae ( Xoo ) Philippine race 6 ( PR6 ) is unable to cause bacterial blight disease on rice lines containing the rice resistance gene Xa21 but is virulent on non-Xa21 rice lines , indicating that PR6 carries avirulence ( avrXa21 ) determinants required for recognition by XA21 .
[ Sen. 4, subscore: 1.00 ]: Xoo PR6 strains carrying disruptions in either gene , PR6DeltaraxP or PR6DeltaraxQ , are unable to produce APS and PAPS and are virulent on Xa21-containing rice lines .
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Score: 1.00 |
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| Title: [ Genome analysis of transgenic homozygous line "Minghui 63-Xa21" ] | | Journal: Sheng Wu Gong Cheng Xue Bao | | Literature: oryza Field: abstract Doc ID: pub11977586 | Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: This indicated that the introduction of Xa21 into the genome of "Minghui 63" did not change these 550 genome sites and their heredity .
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Score: 4.00 |
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| Title: [ Genetic mapping of T-DNA integration sites in Xa21 transgenic rice ] | | Journal: Yi Chuan Xue Bao | | Literature: oryza Field: abstract Doc ID: pub12561472 | Matching Sentences:
[ Sen. 7, subscore: 2.00 ]: The genetic mapping of T-DNA integration sites in Xa21 transgenic rice will benefit the study of position effect and stable inheritance of the transgene Xa21 .
[ Sen. 2, subscore: 1.00 ]: In the previous research we have transferred the Xa21 gene into five rice varieties of China , using Agrobacterium-mediated trasformation .
[ Sen. 3, subscore: 1.00 ]: In this study , T-DNA flanking sequences of Xa21 transgenic rice lines were obtained by using thermal asymmetric interlaced PCR ( TAIL-PCR ) .
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Score: 1.00 |
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| Title: Pyramiding transgenes for multiple resistance in rice against bacterial blight , yellow stem borer and sheath blight .
| | Journal: Theor . Appl . Genet . | | Literature: oryza Field: abstract Doc ID: pub12582865 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: The Xa21 gene ( resistance to bacterial blight ) , the Bt fusion gene ( for insect resistance ) and the chitinase gene ( for tolerance of sheath blight ) were combined in a single rice line by reciprocal crossing of two transgenic homozygous IR72 lines .
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Score: 1.00 |
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| Title: Isolation and linkage analysis of expressed disease-resistance gene analogues of sugar beet ( Beta vulgaris L ) .
| | Journal: Genome | | Literature: oryza Field: abstract Doc ID: pub12669798 | Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: Among the remaining RGAs , 19 revealed similarity to the serine ( threonine ) protein kinase domain of R genes , 4 showed features related to the LRR region of the rice disease resistance gene Xa21 , 1 RGA resembled the sugar beet nematode resistance gene Hs1pro-1 , and 2 had homologies to other gene products associated with disease resistance .
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Score: 1.00 |
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| Title: Cytological and molecular analysis of the Hordeum vulgare-Puccinia triticina nonhost interaction .
| | Journal: Mol .
Plant Microbe Interact .
| | Literature: oryza Field: abstract Doc ID: pub12848428 | Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: Northern analysis indicated that two candidate genes , including a barley ortholog of the rice resistance gene Xa21 , are putatively involved in nonhost and non-race-specific resistance reactions .
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Score: 8.00 |
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| Title: Genetic and physical analysis of the rice bacterial blight disease resistance locus , Xa21 .
| | Journal: Mol . Gen . Genet . | | Literature: oryza Field: abstract Doc ID: pub1362973 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: One chromosome 11 marker ( RG103 ) detected polymorphism between the NILs that cosegregated with Xa21 .
[ Sen. 3, subscore: 1.00 ]: All other chromosome 11 DNA markers tested were monomorphic between the NILs , localizing the Xa21 introgressed region to an 8 . 3 cM interval on chromosome 11 .
[ Sen. 5, subscore: 1.00 ]: Genomic sequences hybridizing with RAPD818 , RAPD248 and RG103 were duplicated specifically in the Xa21 NIL .
[ Sen. 6, subscore: 1.00 ]: All three markers cosegregated with the resistance locus , Xa21 , in a F2 population of 386 progeny .
[ Sen. 7, subscore: 1.00 ]: Based on the frequency with which we recovered polymorphic Xa21-linked markers , we estimated the physical size of the introgressed region to be approximately 800 kb .
[ Sen. 8, subscore: 1.00 ]: This estimation was supported by physical mapping ( using pulsed field gel electrophoresis ) of the sequences hybridizing with the three Xa21-linked DNA markers .
[ Sen. 9, subscore: 1.00 ]: The results showed that the three Xa21-linked markers are physically close to each other , with one copy of the RAPD818 sequences located within 60 kb of RAPD248 and the other copy within 270 kb of RG103 .
[ Sen. 10, subscore: 1.00 ]: None of the enzymes tested generated a DNA fragment that hybridized with all three of the markers indicating that the introgressed region containing the resistance locus Xa21 is probably larger than 270 kb .
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Score: 4.00 |
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| Title: Cloning and characterization of receptor kinase class disease resistance gene candidates in Citrus .
| | Journal: Theor . Appl . Genet . | | Literature: oryza Field: abstract Doc ID: pub13679986 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The rice gene Xa21 represents a unique class of plant disease resistance ( R ) genes with distinct protein structure and broad-spectrum specificity ; few sequences or genes of this class have been cloned and characterized in other plant species .
[ Sen. 2, subscore: 1.00 ]: Degenerate primers were designed from the conserved motifs in the kinase domains of Xa21 and tomato Pto , and used in PCR amplification to identify this class of resistance gene candidate ( RGC ) sequences from citrus for future evaluation of possible association with citrus canker resistance .
[ Sen. 3, subscore: 1.00 ]: Twenty-nine RGC sequences highly similar to the kinase domain of Xa21 ( 55%-60% amino-acid identity ) were cloned and characterized .
[ Sen. 8, subscore: 1.00 ]: A primer-walking strategy was employed to derive full-length gene structures from two BAC clones ; both sequences 17o6RLK and 26m19RLK contained all the features of the rice Xa21 protein , including a signal peptide , the same number of leucine-rich-repeats , and transmembrane and kinase domains .
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Score: 8.00 |
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| Title: Isolation and characterization of rice mutants compromised in Xa21-mediated resistance to X oryzae pv . oryzae .
| | Journal: Theor . Appl . Genet . | | Literature: oryza Field: abstract Doc ID: pub14523518 | Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: To identify genes essential for the function of the Xa21 gene , 4 , 500 IRBB21 ( Xa21 isogenic line in IR24 background ) mutants , induced by diepoxybutane and fast neutrons , were screened against Philippine race six ( PR6 ) Xoo for a change from resistance to susceptibility .
[ Sen. 1, subscore: 1.00 ]: The rice gene , Xa21 , confers resistance to diverse races of Xanthomonas oryzae pv . oryzae ( Xoo ) and encodes a receptor-like kinase with leucine-rich repeats in the extra-cellular domain .
[ Sen. 4, subscore: 1.00 ]: All fully susceptible mutants carried rearrangements at the Xa21 locus as detected by PCR and Southern hybridization .
[ Sen. 5, subscore: 1.00 ]: For the partially susceptible mutants , no changes were detected at the Xa21 locus based on Southern and PCR analyses .
[ Sen. 6, subscore: 1.00 ]: However , two of these mutants were confirmed via genetic analysis to have mutations at the Xa21 locus .
[ Sen. 7, subscore: 1.00 ]: Partially susceptible mutants exhibited variation in level of susceptibility to different Xoo strains , suggesting that they may carry different mutations required for the Xa21-mediated resistance .
[ Sen. 8, subscore: 1.00 ]: The mutants identified in this study provide useful materials for dissecting the Xa21-mediated resistance pathway in rice .
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Score: 1.00 |
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| Title: Isolation and characterization of novel defense response genes involved in compatible and incompatible interactions between rice and Magnaporthe grisea .
| | Journal: Theor . Appl . Genet . | | Literature: oryza Field: abstract Doc ID: pub14605807 | Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: Interestingly , all but one of the identified genes are reported here for the first time to be involved in defense response to rice blast In addition , hybridization of these clones with cDNAs synthesized from RNA samples from bacterial blight-infected leaves showed that few of them are induced or repressed in Xa21 or Xa7-resistant plants , suggesting a minimum overlap of defense responses mediated by different resistance genes to fungal and bacterial pathogens at an early stage of infection .
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Score: 1.00 |
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| Title: Characterisation of early transcriptional changes involving multiple signalling pathways in the Mla13 barley interaction with powdery mildew ( Blumeria graminis f . sp . hordei ) .
| | Journal: Planta | | Literature: oryza Field: abstract Doc ID: pub14648226 | Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: Moreover , bmi7 , similar to rice resistance gene Xa21 , was rapidly up-regulated in both compatible and incompatible interactions , but was then down-regulated by 5 hpi in the virulent interaction .
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Score: 8.00 |
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| Title: Analysis of T-DNA Xa21 loci and bacterial blight resistance effects of the transgene Xa21 in transgenic rice .
| | Journal: Theor . Appl . Genet . | | Literature: oryza Field: abstract Doc ID: pub15088086 | Matching Sentences:
[ Sen. 10, subscore: 2.00 ]: On the basis of genetic mapping of the T-DNA Xa21 loci , the BB resistance effects of the transgene Xa21 at different chromosome locations were investigated using homozygous transgenic lines with only one copy of the transgene .
[ Sen. 1, subscore: 1.00 ]: The genetic loci and phenotypic effects of the transgene Xa21 , a bacterial blight ( BB ) resistance gene cloned from rice , were investigated in transgenic rice produced through an Agrobacterium-mediated transformation system .
[ Sen. 2, subscore: 1.00 ]: The flanking sequences of integrated T-DNAs were isolated from Xa21 transgenic rice lines using thermal asymmetric interlaced PCR .
[ Sen. 3, subscore: 1.00 ]: Based on the analysis of 24 T-DNA Xa21 flanking sequences , T-DNA loci in rice could be classified into three types : the typical T-DNA integration with the definite left and right borders , the T-DNA integration linked with the adjacent vector backbone sequences and the T-DNA integration involved in a complicated recombination in the flanking sequences .
[ Sen. 5, subscore: 1.00 ]: All three types of integrated transgene Xa21 could be stably inherited and expressed the BB resistance through derived generations in their respective transgenic lines .
[ Sen. 11, subscore: 1.00 ]: Among the transgenic lines , no obvious position effects of the transgene Xa21 were observed .
[ Sen. 12, subscore: 1.00 ]: In addition , the BB resistance levels of the Xa21 transgenic plants with different transgene copy numbers and on different genetic backgrounds were also investigated .
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Score: 3.00 |
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| Title: Bacterial genes involved in type I secretion and sulfation are required to elicit the rice Xa21-mediated innate immune response .
| | Journal: Mol .
Plant Microbe Interact .
| | Literature: oryza Field: abstract Doc ID: pub15195942 | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: The rice Xa21 gene encodes a receptor-like kinase that provides immunity against strains of the bacterial pathogen Xanthomonas oryzae pv . oryzae carrying AvrXa21 activity .
[ Sen. 6, subscore: 1.00 ]: Sequence analysis of three naturally occurring X oryzae pv . oryzae strains no longer recognized by Xa21 revealed alterations in the raxST and raxA genes .
[ Sen. 8, subscore: 1.00 ]: These results indicate that bacterial type I secretion is necessary for Xa21-mediated recognition and immunity and further suggest that type I secretion and modification of pathogen-associated molecules play an important role in triggering the innate immune response in rice .
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Score: 3.00 |
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| Title: RaxH/RaxR : a two-component regulatory system in Xanthomonas oryzae pv . oryzae required for AvrXa21 activity .
| | Journal: Mol .
Plant Microbe Interact .
| | Literature: oryza Field: abstract Doc ID: pub15195943 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: X oryzae pv . oryzae Philippine race 6 ( PR6 ) strains are unable to establish infection in rice lines expressing the resistance gene Xa21 .
[ Sen. 3, subscore: 1.00 ]: Although the pathogen-associated molecule that triggers the Xa21-mediated defense response ( AvrXa21 ) is unknown , six rax ( required for AvrXa21 activity ) genes encoding proteins involved in sulfur metabolism and Type I secretion were recently identified .
[ Sen. 5, subscore: 1.00 ]: Null mutants of PR6 strain PXO99 that are impaired in either raxR , raxH , or both cause lesions significantly longer and grow to significantly higher levels than does the wild-type strain in Xa21-rice leaves .
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Score: 1.00 |
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| Title: Bacterial artificial chromosome ( BAC ) library resource for positional cloning of pest and disease resistance genes in cassava ( Manihot esculenta Crantz ) .
| | Journal: Plant Mol . Biol . | | Literature: oryza Field: abstract Doc ID: pub15630619 | Matching Sentences:
[ Sen. 8, subscore: 1.00 ]: To demonstrate the potential of the libraries , the TMS30001 library was screened by southern hybridization using a cassava analog ( CBB1 ) of the Xa21 gene from rice that maps to a region containing a QTL for resistance to CBB as probe .
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Score: 3.00 |
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| Title: [ Analysis of the transgenic rice plants derived from transformed anther calli ] | | Journal: Yi Chuan Xue Bao | | Literature: oryza Field: abstract Doc ID: pub15633644 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Rice calli derived from anther culture were used as recipient to transfer a rice blight resistance gene , Xa21 , into a japonica rice variety , Taipei 309 , via Agrobacterium-mediated transformation .
[ Sen. 3, subscore: 1.00 ]: PCR , Southern blot , FISH and blight resistance analysis all indicated that Xa21 gene has been integrated into the T0 plant genomes .
[ Sen. 5, subscore: 1.00 ]: Chi2 test showed that two T1 populations segregated with a ratio of 3 : 1 , indicating that a single copy of Xa21 gene was integrated into the genome , whereas the segregation ratios of the other two T1 populations were non-Mendelian .
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Score: 2.00 |
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| Title: [ Study on the teratogenicity effects of genetically modified rice with Xa21 on rats ] | | Journal: | | Literature: oryza Field: abstract Doc ID: pub15727184 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: OBJECTIVE : To observe the effects of genetically modified rice with Xa21 on the development of rat embryos .
[ Sen. 8, subscore: 1.00 ]: CONCLUSION : Compared with the non-transgenic rice , transgenic rice modified with Xa21 gene showed no significant differences in rat pregnant rate and embryo development .
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Score: 2.00 |
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| Title: [ Effects of transgenes insertion on pollen vigor and hybrid seed set of rice ] | | Journal: | | Literature: oryza Field: abstract Doc ID: pub15852969 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: The in vitro pollen germination rates of five transgenic rice lines transformed respectively with bar , crylAb , BADH and Xa21 gene were investigated , and compared with their relative receptor rice varieties .
[ Sen. 5, subscore: 1.00 ]: Investigation on the hybrid seed set of 26 hand-crosses showed that the inserted bar or crylAb gene had a significant effect on the hybrid seed set of receptors , while the effect of Xa21 gene was smaller .
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Score: 6.00 |
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| Title: [ Accelerated improvement of bacterial blight resistance of Shuhui527 using molecular marker-assisted selection ] | | Journal: Sheng Wu Gong Cheng Xue Bao | | Literature: oryza Field: abstract Doc ID: pub15966313 | Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: The objective of this study is to improve the BB resistance of Shuhui527 by introgressing Xa21 and Xa4 , the two broad-spectrum BB resistance genes , into Shuhui527 with IRBB60 as the donor , pTA248 and MP12 , linking tightly with Xa21 and Xa4 respectively as DNA markers .
[ Sen. 3, subscore: 1.00 ]: The IRBB60 , from the International Rice Research Institute ( IRRI ) , contains dominant genes Xa21 and Xa4 conferring resistance to BB .
[ Sen. 5, subscore: 1.00 ]: BC1 F1 progenies of ( Shuhui527 x IRBB60 ) , containing Xa21 and Xa4 identified using PCR screening and with agronomic traits including plant type , grain type and days to heading etc similar to those of Shuhui527 , were subsequently backcrossed to Shuhui527 and self-pollinated to generate BC2 F1 and BC1 F2 . The BC3 F1 and BC3 F2 were subsequently developed using the same approach .
[ Sen. 6, subscore: 1.00 ]: Among the 20 BC3 F2 plants , homozygous Xa21 and Xa4 , 10 plants were the most similar to Shuhui527 in the agronomic traits , and were screened using 120 pairs SSR and 100 pairs RAPD markers .
[ Sen. 9, subscore: 1.00 ]: The evaluation of the polymorphisms and selection accuracies of pTA248 and MP12 demonstrated that the polymorphisms of the two markers were obvious and co-dominant and the accuracies were more than 97% and 83% respectively , indicating the two markers are good for Xa21 and Xa4 in Molecular Marker-assisted Selection .
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Score: 4.00 |
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| Title: [ Isolation and Sequence Analysis of the Xa21 Gene Wxon II Homologus from Different Species of Wild Rice in Yunnan . ] | | Journal: Yi Chuan | | Literature: oryza Field: abstract Doc ID: pub15985401 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The Xa21 gene previously cloned from the wild rice species Oryzae longistaminata confers broad-spectrum resistance to rice leaf blight caused by different strains of Xanthomonas oryzae pv . oryzae .
[ Sen. 2, subscore: 1.00 ]: Here we attempted to determine the existence of Xa21 homologs in other wild rice species and rice cultivars and the sequence differences between the homologs .
[ Sen. 3, subscore: 1.00 ]: We synthesized specific primers based on the reported Xa21 sequence to amplify homologs of the gene exon II from several rice cultivars and three wild rice species in Yunnan Province , China .
[ Sen. 4, subscore: 1.00 ]: The fragments cloned from various types of O rufipogon Griff from Jinghong and Yuanjiang , Yunnan Province , were highly homologous to the reported Xa21 gene exon II .
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Score: 3.00 |
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| Title: Rice NRR , a negative regulator of disease resistance , interacts with Arabidopsis NPR1 and rice NH1 .
| | Journal: Plant J | | Literature: oryza Field: abstract Doc ID: pub16115061 | Matching Sentences:
[ Sen. 12, subscore: 2.00 ]: The fact that NRR compromises Xa21-mediated resistance indicates cross-talk or overlap between NH1 and Xa21-mediated pathways .
[ Sen. 8, subscore: 1.00 ]: When constitutively over-expressed in rice , NRR affected basal resistance , age-related resistance and Xa21-mediated resistance , causing enhanced susceptibility to Xoo .
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Score: 1.00 |
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| Title: Plant and animal pathogen recognition receptors signal through non-RD kinases .
| | Journal: PLoS Pathog .
| | Literature: oryza Field: abstract Doc ID: pub16424920 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: PRRs commonly associate with or contain members of a monophyletic group of kinases called the interleukin-1 receptor-associated kinase ( IRAK ) family that include Drosophila Pelle , human IRAKs , rice XA21 and Arabidopsis FLS2 .
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Score: 7.00 |
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| Title: The autophosphorylated Ser686 , Thr688 , and Ser689 residues in the intracellular juxtamembrane domain of XA21 are implicated in stability control of rice receptor-like kinase .
| | Journal: Plant J | | Literature: oryza Field: abstract Doc ID: pub16460508 | Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: Xa21 encodes a receptor-like kinase ( XA21 ) .
[ Sen. 1, subscore: 1.00 ]: The rice gene Xa21 confers resistance against Xanthomonas oryzae pv . oryzae ( Xoo ) .
[ Sen. 3, subscore: 1.00 ]: We demonstrate that XA21 autophosphorylates residues Ser686 , Thr688 and Ser689 in vitro .
[ Sen. 5, subscore: 1.00 ]: Plants carrying these same substitutions in XA21 were compromised in their resistance to the normally avirulent Xoo Philippine race 6 .
[ Sen. 6, subscore: 1.00 ]: Additionally , we show that wild-type XA21 and the kinase-dead mutant with the invariable Lys736 residue mutated to glutamic acid were also proteolytically degraded in protein extracts .
[ Sen. 8, subscore: 1.00 ]: We propose that autophosphorylation of Ser686 , Thr688 and Ser689 functions to stabilize XA21 against the developmentally controlled proteolytic activity .
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Score: 5.00 |
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| Title: [ Generation of selectable marker-free and vector backbone sequence-free Xa21 transgenic rice ] | | Journal: Sheng Wu Gong Cheng Xue Bao | | Literature: oryza Field: abstract Doc ID: pub16607944 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The dominant gene Xa21 with broad-spectrum and high resistance to Xanthomonas oryzae pv . oryzae ( Xoo ) was transferred into C418 , an important restorer line of japonica hybrid rice in China using double right-border ( DRB ) T-DNA binary vector through Agrobacterium-mediated transformation .
[ Sen. 2, subscore: 1.00 ]: 17 transgenic lines were Xa21-positive with high resistance to the race P6 of Xoo through PCR analysis and resistance identification , among the total 27 independent primary transformants ( T0 ) obtained .
[ Sen. 3, subscore: 1.00 ]: The subsequent analysis of the T1 progenies of these 17 T0 lines through PCR-assisted selection and resistance investigation showed that four Xa21 transgenic T0 lines could produce selectable marker-free ( SMF ) progenies .
[ Sen. 5, subscore: 1.00 ]: In addition , PCR analysis also revealed these SMF progenies did not contain vector backbone sequence , and they were named as SMF and vector backbone sequence-free ( SMF-VBSF ) Xa21 transgenic plants .
[ Sen. 6, subscore: 1.00 ]: The further molecular and phenotypic analysis of the T2 and T3 progenies testified the homozygous SMF-VBSF Xa21 transgenic plants were obtained with high resistance to Xoo .
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Score: 5.00 |
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| Title: [ Advances of rice bacterial blight disease resistance gene Xa21 ] | | Journal: Yi Chuan | | Literature: oryza Field: abstract Doc ID: pub16818441 | Matching Sentences:
[ Sen. 3, subscore: 2.00 ]: The molecular mechanism of resistance and its breeding application , including biochemical properties of the kinase domain of XA21 , AvrXa21 identification , the disease resistance pathway mediated by Xa21 , and the mechanism of disease resistance were summarized .
[ Sen. 1, subscore: 1.00 ]: Gene Xa21 is the first disease resistance gene cloned from rice ( Oryza sativa L ) , which encodes a receptor-like kinase and confers broad spectrum resistance against Xanthomonas oryzae pv . oryzae ( Xoo ) .
[ Sen. 2, subscore: 1.00 ]: In this paper , the discovery , mapping and cloning of Xa21 were reviewed briefly .
[ Sen. 4, subscore: 1.00 ]: Future perspective on Xa21-related research is also discussed .
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Score: 2.00 |
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| Title: From the Academy : Colloquium review .
Unique characteristics of Xanthomonas oryzae pv . oryzae AvrXa21 and implications for plant innate immunity .
| | Journal: Proc . Natl . Acad . Sci . USA | | Literature: oryza Field: abstract Doc ID: pub17082309 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: The rice pathogen recognition receptor , XA21 , confers resistance to Xanthomonas oryzae pv . oryzae strains producing the AvrXa21 elicitor .
[ Sen. 3, subscore: 1.00 ]: Xa21 codes for a receptor-like kinase consisting of an extracellular leucine-rich repeat domain , a transmembrane domain , and a cytoplasmic kinase domain .
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Score: 2.00 |
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| Title: Adapting rice anther culture to gene transformation and RNA interference .
| | Journal: Sci .
China , C , Life Sci .
| | Literature: oryza Field: abstract Doc ID: pub17172048 | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: Using this protocol , we obtained 145 independent green transformants from five cultivars of japonica rice by transformation with a binary vector pCXK1301 bearing the rice gene , Xa21 for resistance to bacterial blight , of which 140 were further confirmed by PCR and Southern hybridization analysis , including haploids ( 32 . 1% ) , diploids ( 62 . 1% ) and mixoploids ( 7 . 5% ) .
[ Sen. 5, subscore: 1.00 ]: Finally , by including 28 from colchicine induced or spontaneous diploidization of haploids later after transformation , a total of 43 doubled haploids ( 30 . 7% ) of Xa21 transgenic lines were obtained .
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Score: 9.00 |
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| Title: Rice XA21 Binding Protein 3 Is a Ubiquitin Ligase Required for Full Xa21-Mediated Disease Resistance .
| | Journal: | | Literature: oryza Field: abstract Doc ID: pub17172358 | Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: We identified XA21 binding protein 3 ( XB3 ) , an E3 ubiquitin ligase , as a substrate for the XA21 Ser and Thr kinase .
[ Sen. 3, subscore: 2.00 ]: The interaction between XB3 and the kinase domain of XA21 has been shown in yeast and in vitro , and the physical association between XB3 and XA21 in vivo has also been confirmed by coimmunoprecipitation assays .
[ Sen. 7, subscore: 2.00 ]: These results indicate that Xb3 is necessary for full accumulation of the XA21 protein and for Xa21-mediated resistance .
[ Sen. 1, subscore: 1.00 ]: XA21 is a receptor-like kinase protein in rice ( Oryza sativa ) that confers gene-for-gene resistance to specific races of the causal agent of bacterial blight disease , Xanthomonas oryzae pv oryzae .
[ Sen. 4, subscore: 1.00 ]: XB3 contains an ankyrin repeat domain and a RING finger motif that is sufficient for its interaction with the kinase domain of XA21 and for its E3 ubiquitin ligase activity , respectively .
[ Sen. 6, subscore: 1.00 ]: Furthermore , reduced levels of Xb3 lead to decreased levels of the XA21 protein .
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Score: 2.00 |
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| Title: Pyramiding transgenic resistance in elite indica rice cultivars against the sheath blight and bacterial blight .
| | Journal: | | Literature: oryza Field: abstract Doc ID: pub17221225 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Elite indica rice cultivars were cotransformed with genes expressing a rice chitinase ( chi11 ) and a thaumatin-like protein ( tlp ) conferring resistance to fungal pathogens and a serine-threonine kinase ( Xa21 ) conferring bacterial blight resistance , through particle bombardment , with a view to pyramiding sheath blight and bacterial blight resistance .
[ Sen. 5, subscore: 1.00 ]: A transgenic Pusa Basmati1 line pyramided with chi11 , tlp , and Xa21 showed an enhanced resistance to both sheath blight and bacterial blight .
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Score: 2.00 |
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| Title: Proteomic analysis of rice plasma membrane reveals proteins involved in early defense response to bacterial blight .
| | Journal: | | Literature: oryza Field: abstract Doc ID: pub17407182 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: By using an improved method of PM protein preparation and PM-bound green fluorescent protein fusion protein as a visible marker , we conducted PM proteomic analysis of the rice suspension cells expressing the disease resistance gene Xa21 , to identify PM components involved in the early defense response to bacterial blight ( Xanthomonas oryzae pv . oryzae ) .
[ Sen. 3, subscore: 1.00 ]: A total of 20 regulated protein spots were observed on 2-D gels of PM fractions at 12 and 24 h after pathogen inoculation , of which some were differentially regulated between the incompatible and compatible interactions mediated by Xa21 , with good correlation between biological repeats .
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Score: 6.00 |
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| Title: Epigenetic inheritance in rice plants .
| | Journal: Ann Bot ( Lond ) | | Literature: oryza Field: abstract Doc ID: pub17576658 | Matching Sentences:
[ Sen. 12, subscore: 2.00 ]: The other clone encoded an Xa21-like protein , Xa21G .
[ Sen. 15, subscore: 2.00 ]: When infected with X oryzae pv . oryzae , against which Xa21 confers resistance in a gene-for-gene manner , the progeny of Line-2 were apparently resistant while the wild type was highly susceptible without Xa21G expression .
[ Sen. 14, subscore: 1.00 ]: Expression of Xa21G was not detectable in wild type but was constitutive in Line-2 .
[ Sen. 16, subscore: 1.00 ]: CONCLUSIONS : These results indicated that demethylation was selective in Line-2 , and that promoter demethylation abolished the constitutive silencing of Xa21G due to hypermethylation , resulting in acquisition of disease resistance .
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Score: 2.00 |
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| Title: The Xanthomonas oryzae pv . oryzae PhoPQ two-component system is required for AvrXA21 activity , hrpG expression , and virulence .
| | Journal: J Bacteriol | | Literature: oryza Field: abstract Doc ID: pub18203830 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The rice pathogen recognition receptor , XA21 , confers resistance to Xanthomonas oryzae pv . oryzae strains producing the type one system-secreted molecule , AvrXA21 .
[ Sen. 8, subscore: 1.00 ]: Growth curve analysis revealed that AvrXA21 activity is impaired in a phoQ knockout strain as reflected by enhanced growth of this strain in rice lines carrying XA21 .
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Score: 6.00 |
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| Title: [ Expression and autophosphorylation analysis of XA21 and PI-D2 protein kinases in Saccharomyces cerevisiae ] | | Journal: Wei Sheng Wu Xue Bao | | Literature: oryza Field: abstract Doc ID: pub18271255 | Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: Xa21 confers resistance to bacterial blight , while Pi-d2 confers resistance to rice blast Both Xa21 and Pi-d2 encode receptor kinase-like proteins .
[ Sen. 3, subscore: 1.00 ]: Biochemical properties of XA21 kinase expressed in bacterial were characterized in our previous report .
[ Sen. 4, subscore: 1.00 ]: In this study , both XA21 and PI-D2 kinase domain were PCR amplified and cloned into yeast expression vector pEGH via recombinational cloning strategy , kinase proteins expressed in eukaryotic yeast system was purified and autophosphorylation assay was carried out .
[ Sen. 5, subscore: 1.00 ]: The results indicated that XA21 and PI-D2 protein can be detected by SDS-PAGE and showed expected molecular weight .
[ Sen. 6, subscore: 1.00 ]: Autophosphorylation assay indicated that yeast expressed XA21 and PI-D2 were active when incubated with P32 labelled ATP .
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Score: 1.00 |
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| Title: Cloning and expression of resistance gene analogs ( RGAs ) from wild banana resistant to banana Fusarium wilt .
| | Journal: Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao | | Literature: oryza Field: abstract Doc ID: pub18349511 | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: The deduced amino acids of other four RGAs ( WST1 , WST2 , WST3 and WST4 ) all contain the serine/threonine kinase domain and WST3 encodes a polypeptide homologous to that of bacterial blight resistance gene Xa21 of rice .
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Score: 9.00 |
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| Title: Rice XB15 , a protein phosphatase 2C , negatively regulates cell death and XA21-mediated innate immunity .
| | Journal: PLoS Biol | | Literature: oryza Field: abstract Doc ID: pub18817453 | Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: A yeast two-hybrid screen using the intracellular portion of XA21 , including the juxtamembrane ( JM ) and kinase domain as bait , identified a protein phosphatase 2C ( PP2C ) , called XA21 binding protein 15 ( XB15 ) .
[ Sen. 3, subscore: 1.00 ]: The rice ( Oryza sativa L ) XA21 receptor kinase is a key recognition and signaling determinant in the innate immune response .
[ Sen. 5, subscore: 1.00 ]: The interaction of XA21 and XB15 was confirmed in vitro and in vivo by glutathione-S-transferase ( GST ) pull-down and co-immunoprecipitation assays , respectively .
[ Sen. 7, subscore: 1.00 ]: Autophosphorylated XA21 can be dephosphorylated by XB15 in a temporal and dosage-dependent manner .
[ Sen. 8, subscore: 1.00 ]: A serine residue in the XA21 JM domain is required for XB15 binding .
[ Sen. 9, subscore: 1.00 ]: Xb15 mutants display a severe cell death phenotype , induction of pathogenesis-related genes , and enhanced XA21-mediated resistance .
[ Sen. 10, subscore: 1.00 ]: Overexpression of Xb15 in an XA21 rice line compromises resistance to the bacterial pathogen Xanthomonas oryzae pv . oryzae .
[ Sen. 11, subscore: 1.00 ]: These results demonstrate that Xb15 encodes a PP2C that negatively regulates the XA21-mediated innate immune response .
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Score: 2.00 |
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| Title: Introduction of bacterial blight resistance into Triguna , a high yielding , mid-early duration rice variety .
| | Journal: Biotechnol J | | Literature: oryza Field: abstract Doc ID: pub19253322 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: We have used molecular marker-assisted selection in a backcross breeding program to introgress three genes ( Xa21 , xa13 , and xa5 ) for BB resistance into Triguna , a mid-early duration , high yielding rice variety that is susceptible to BB .
[ Sen. 5, subscore: 1.00 ]: Plants containing the two-gene combination , Xa21 and xa13 , were found to exhibit excellent resistance against BB .
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Score: 6.00 |
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| Title: Dissection of the factors affecting development-controlled and race-specific disease resistance conferred by leucine-rich repeat receptor kinase-type R genes in rice .
| | Journal: Theor Appl Genet | | Literature: oryza Field: abstract Doc ID: pub19390838 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Xa3/Xa26 and Xa21 , encoding leucine-rich repeat ( LRR ) -kinase type plasma membrane proteins , mediate race-specific resistance to Xanthomonas oryzae pv . oryzae ( Xoo ) , which causes bacterial blight , one of the most devastating rice diseases .
[ Sen. 3, subscore: 1.00 ]: Plants carrying Xa3/Xa26 and plants carrying Xa21 have different resistance spectra and the functions of the two R genes are regulated by developmental stage .
[ Sen. 4, subscore: 1.00 ]: Four chimeric genes encoding proteins consisting of different parts of XA3/XA26 and XA21 were constructed by domain swapping and transformed into a susceptible rice variety .
[ Sen. 5, subscore: 1.00 ]: The resistance spectra and development-regulated resistance of the transgenic plants carrying Xa3/Xa26 , Xa21 , or chimeric gene to different Xoo strains were analyzed in the same genetic background .
[ Sen. 6, subscore: 1.00 ]: The results suggest that the gradually increased expression of Xa3/Xa26 and Xa21 plays an important role in the progressively enhanced Xoo resistance during rice development .
[ Sen. 7, subscore: 1.00 ]: In addition , the LRR domains of XA3/XA26 and XA21 are important determinants of race-specific recognition during rice-Xoo interaction , but juxtamembrane regions of the two R proteins also appear to contribute to resistance specificity .
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Score: 1.00 |
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| Title: Proteome analysis of detergent-resistant membranes ( DRMs ) associated with OsRac1-mediated innate immunity in rice .
| | Journal: Plant Cell Physiol | | Literature: oryza Field: abstract Doc ID: pub19502382 | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: We identified 192 proteins in DRMs that included receptor-like kinases ( RLKs ) such as Xa21 , nucleotide-binding leucine-rich repeat ( NB-LRR ) -type disease resistance proteins , a glycosylphosphatidylinositol ( GPI ) -anchored protein , syntaxin , NADPH oxidase , a WD-40 repeat family protein and various GTP-binding proteins .
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Score: 4.00 |
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| Title: [ Application of competitive PCR for screening selectable marker-free Xa21 transgenic rice ] | | Journal: Sheng Wu Gong Cheng Xue Bao | | Literature: oryza Field: abstract Doc ID: pub19637639 | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: The competitive template of Xa21 was the endogenous Xa2l homologous sequence located on chromosome 11 .
[ Sen. 6, subscore: 1.00 ]: Through competitive PCR analysis of transgenic T1 plants produced by double right border binary vector , false positive or false negative samples were effectively diminished , and genuine SMF Xa21 transgenic plants were obviously obtained .
[ Sen. 7, subscore: 1.00 ]: Comparing with the conventional non-competitive PCR , competitive PCR increased the accuracy for selecting SMF Xa21 transgenic plants .
[ Sen. 8, subscore: 1.00 ]: The results of bacterial blight ( BB ) resistance tests and hygromycin B resistance assay of SMF Xa21 transgenic plants testified the reliability of this method .
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