| 63 matches found in 28 documents. Search time: 0.002 seconds. |
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Score: 1.00 |
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| Title: A telomeric avirulence gene determines efficacy for the rice blast resistance gene Pi-ta .
| | Journal: Plant Cell | | Literature: oryza Field: abstract Doc ID: pub11090206 | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: Analysis of spontaneous avr-pita ( - ) mutants indicated that the gene is located in a telomeric 6 . 5-kb BglII restriction fragment .
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Score: 3.00 |
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| Title: Beta-glucosidase , exo-beta-glucanase and pyridoxine transglucosylase activities of rice BGlu1 .
| | Journal: Biochem .
J | | Literature: oryza Field: abstract Doc ID: pub14692878 | Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: The bglu1 cDNA for a beta-glucosidase cloned from rice ( Oryza sativa L ) seedlings was expressed as a soluble and active protein in Escherichia coli and designated BGlu1 .
[ Sen. 6, subscore: 1.00 ]: BGlu1 also had high transglucosylation activity towards pyridoxine , producing pyridoxine 5-O-beta-D-glucopyranoside in the presence of the glucose donor p-nitrophenyl beta-D-glucoside .
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Score: 4.00 |
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| Title: Purification , crystallization and preliminary X-ray analysis of rice BGlu1 beta-glucosidase with and without 2-deoxy-2-fluoro-beta-D-glucoside .
| | Journal: Acta Crystallograph . Sect . F Struct . Biol . Cryst Commun .
| | Literature: oryza Field: abstract Doc ID: pub16880561 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Rice ( Oryza sativa ) BGlu1 beta-glucosidase was expressed in Escherichia coli with N-terminal thioredoxin and hexahistidine tags and purified by immobilized metal-affinity chromatography ( IMAC ) .
[ Sen. 2, subscore: 1.00 ]: After removal of the N-terminal tags , cation-exchange and S-200 gel-filtration chromatography yielded a 50 kDa BGlu1 with >95% purity .
[ Sen. 4, subscore: 1.00 ]: Small tetragonal crystals of BGlu1 with and without inhibitor grew in 18% ( w/v ) PEG 8000 with 0 . 1 M sodium cacodylate pH 6 . 5 and 0 . 2 M zinc acetate .
[ Sen. 5, subscore: 1.00 ]: Crystals of BGlu1 with inhibitor were streak-seeded into 23% ( w/v ) PEG MME 5000 , 0 . 2 M ammonium sulfate , 0 . 1 M MES pH 6 . 7 to yield larger crystals .
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Score: 4.00 |
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| Title: Enzymatic synthesis of cello-oligosaccharides by rice BGlu1 { beta } -glucosidase glycosynthase mutants .
| | Journal: | | Literature: oryza Field: abstract Doc ID: pub17405771 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Rice ( BGlu1 ) beta-glucosidase is a glycosyl hydrolase family 1 enzyme that acts as an exoglucanase on beta- ( 1 , 4 ) - and short beta- ( 1 , 3 ) -linked gluco-oligosaccharides .
[ Sen. 2, subscore: 1.00 ]: Mutations of BGlu1 beta-glucosidase at glutamate residue 414 of its natural precursor destroyed the enzymes catalytic activity , but the enzyme could be rescued in the presence of the anionic nucleophiles formate and azide , which verifies that this residue is the catalytic nucleophile .
[ Sen. 8, subscore: 1.00 ]: The BGlu1 E414G glycosynthase was found to prefer longer chain length oligosaccharides that occupy at least three sugar residue binding subsites as acceptors for productive transglucosylation .
[ Sen. 9, subscore: 1.00 ]: This is the first report of a beta-glucansynthase derived from an exoglycosidase that can produce long chain cello-oligosaccharides , which likely reflects the extended oligosaccharide binding site of rice BGlu1 beta-glucosidase .
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Score: 3.00 |
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| Title: Structural insights into rice BGlu1 beta-glucosidase oligosaccharide hydrolysis and transglycosylation .
| | Journal: J Mol Biol | | Literature: oryza Field: abstract Doc ID: pub18308333 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The structures of rice BGlu1 beta-glucosidase , a plant beta-glucosidase active in hydrolyzing cell wall-derived oligosaccharides , and its covalent intermediate with 2-deoxy-2-fluoroglucoside have been solved at 2 . 2 A and 1 . 55 A resolution , respectively .
[ Sen. 5, subscore: 1.00 ]: The coordination of the hydroxyl groups suggests that sugars are positioned as acceptors for transglycosylation by their interactions with E176 , the catalytic acid/base , and Y131 , which is conserved in barley BGQ60/beta-II beta-glucosidase , that has oligosaccharide hydrolysis and transglycosylation activity similar to rice BGlu1 .
[ Sen. 6, subscore: 1.00 ]: As the rice and barley enzymes have different preferences for cellobiose and cellotriose , residues that appeared to interact with docked oligosaccharides were mutated to those of the barley enzyme to see if the relative activities of rice BGlu1 toward these substrates could be changed to those of BGQ60 .
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