| 285 matches found in 115 documents. Search time: 0.006 seconds. |
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| Title: Expression of a gibberellin-induced leucine-rich repeat receptor-like protein kinase in deepwater rice and its interaction with kinase-associated protein phosphatase .
| | Journal: Plant Physiol .
Year: 1999 | | Literature: oryza Field: abstract Doc ID: pub10364408 | Matching Sentences:
[ Sen. 9, subscore: 1.00 ]: In protein-binding assays , OsKAPP interacted with a receptor-like protein kinase , RLK5 of Arabidopsis , but not with the protein kinase domains of the rice and maize receptor-like protein kinases Xa21 and ZmPK1 , respectively .
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Score: 7.00 |
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| Title: Short communication : developmental control of Xa21-mediated disease resistance in rice .
| | Journal: Plant J Year: 1999 | | Literature: oryza Field: abstract Doc ID: pub10571882 | Matching Sentences:
[ Sen. 6, subscore: 3.00 ]: Expression of the Xa21 gene transcript is not correlated with expression of Xa21 disease resistance indicating that the developmental regulation of Xa21-resistance is either controlled post-transcriptionally or by other factors .
[ Sen. 1, subscore: 1.00 ]: The rice resistance gene Xa21 confers resistance against the bacterial pathogen Xanthomonas oryzae pv . oryzae ( Xoo ) .
[ Sen. 2, subscore: 1.00 ]: The molecular genetic mechanism controlling the integration of the Xa21-mediated disease resistance response with the developmental program in rice is under study in this model system .
[ Sen. 4, subscore: 1.00 ]: Xa21-resistance progressively increases from the susceptible juvenile leaf 2 stage through later stages , with 100% resistance at the adult leaf 9/10 stage .
[ Sen. 5, subscore: 1.00 ]: We found that Xa21 expression is independent of plant developmental stage , infection with Xoo , or wounding .
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| Title: Perception of brassinosteroids by the extracellular domain of the receptor kinase BRI1 .
| | Journal: Science Year: 2000 | | Literature: oryza Field: abstract Doc ID: pub10875920 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: The extracellular leucine-rich repeat ( LRR ) and transmembrane domains of the Arabidopsis receptor kinase BRI1 , which is implicated in brassinosteroid signaling , were fused to the serine/threonine kinase domain of XA21 , the rice disease resistance receptor .
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Score: 3.00 |
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| Title: [ Introduction of wide spectrum rice bacterial blight resistance gene Xa21 into two-line genic male sterile rice variety Peiai 64S ] | | Journal: Sheng Wu Gong Cheng Xue Bao Year: 2000 | | Literature: oryza Field: abstract Doc ID: pub10976312 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Agrobacterium-mediated transformation of two-line genic male sterile Indica rice variety Peiai 64S was conducted using a cloned gene , Xa21 , as the foreign gene and mature embryo calli as the recipients .
[ Sen. 3, subscore: 1.00 ]: The PCR analysis and Southern blotting showed the integration of Xa21 gene into the genome the transgenic plants .
[ Sen. 5, subscore: 1.00 ]: Analyses of T1 plants of the tested transgenic lines showed that integrated Xa21 gene could be steadily inherited and segregated in a 3 : 1 ratio .
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| Title: The rice Rim2 transcript accumulates in response to Magnaporthe grisea and its predicted protein product shares similarity with TNP2-like proteins encoded by CACTA transposons .
| | Journal: Mol . Gen . Genet . Year: 2000 | | Literature: oryza Field: abstract Doc ID: pub11016827 | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: A 1 . 05-kb segment of the Rim2 sequence shows 82% nucleotide sequence identity with sequences flanking the A1 and C members of the rice Xa21 disease resistance gene family .
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Score: 2.00 |
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| Title: [ Amplification and analysis of T-DNA flanking sequences in transgenic rice ] | | Journal: Yi Chuan Xue Bao Year: | | Literature: oryza Field: abstract Doc ID: pub11329876 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Using thermal asymmetric interlaced PCR ( TAIL-PCR ) , we analyzed the flanking sequences of T-DNAs in transgenic rice plants , in which the resistance gene for rice bacterial blight disease , Xa21 , had been integrated stably .
[ Sen. 3, subscore: 1.00 ]: Sequence analysis of 24 fragments amplified by TAIL-PCR showed that of them 14 were rice genomic DNA , 9 contained vector backbone sequences , and one was a fragment of the exogenous gene Xa21 .
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| Title: Construction of a 1 . 2-Mb contig including the citrus tristeza virus resistance gene locus using a bacterial artificial chromosome library of Poncirus trifoliata ( L ) Raf .
| | Journal: Genome Year: 2001 | | Literature: oryza Field: abstract Doc ID: pub11444697 | Matching Sentences:
[ Sen. 9, subscore: 1.00 ]: This contig contains several putative disease-resistance genes similar to the rice Xa21 gene , the tomato Cf-2 gene , and the Arabidopsis thaliana RPS2 gene .
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Score: 2.00 |
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| Title: Marker assisted selection of bacterial blight resistance genes in rice .
| | Journal: Biochem . Genet . Year: 2001 | | Literature: oryza Field: abstract Doc ID: pub11590832 | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: The basis of selection of these DNA markers was their close linkage to xa5 , xa13 , and Xa21 genes and their positions on the rice genetic map relative to bacterial blight resistance genes .
[ Sen. 4, subscore: 1.00 ]: Eight lines were found to contain the xa5 gene while two lines contained Xa21 gene and none of the lines contained the xa13 gene with the exception of its near-isogenic line .
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Score: 3.00 |
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| Title: Are the dominant and recessive plant disease resistance genes similar?
A case study of rice R genes and Xanthomonas oryzae pv . oryzae races .
| | Journal: Genetics Year: 2001 | | Literature: oryza Field: abstract Doc ID: pub11606550 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The resistance of rice to its bacterial blight pathogen Xanthomonas oryzae pv . oryzae ( Xoo ) has both qualitative and quantitative components that were investigated using three near-isogenic line sets for four resistance ( R ) genes ( Xa4 , xa5 , xa13 , and Xa21 ) and 12 Xoo races .
[ Sen. 6, subscore: 1.00 ]: Two R genes , Xa4 and Xa21 , showed complete dominance against the avirulent Xoo races and had large residual effects against virulent ones .
[ Sen. 10, subscore: 1.00 ]: There was a strong interaction leading to increased resistance between xa13 and xa5 and between either of them and Xa4 or Xa21 , suggesting their regulatory roles in the rice defensive pathway ( s ) .
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Score: 3.00 |
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| Title: [ A genetically modified japonica restorer line , C418-Xa21 , and its hybrid rice with bacterial blight resistance ] | | Journal: Sheng Wu Gong Cheng Xue Bao Year: 2001 | | Literature: oryza Field: abstract Doc ID: pub11702691 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The cloned bacterial blight ( BB ) resistance gene Xa21 was transferred into C418 , a major restorer line of japonica hybrid rice in China , using an Agrobacterium-mediated system .
[ Sen. 4, subscore: 1.00 ]: The selected transgenic restorer lines were then crossed with a commonly used sterile line , TijinA , to produce Xa21 transgenic hybrid rice .
[ Sen. 8, subscore: 1.00 ]: The propagation of this BB resistant hybrid variety with the transgene Xa21 with extend hybrid rice production in north China .
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Score: 9.00 |
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| Title: Biochemical characterization of the kinase domain of the rice disease resistance receptor-like kinase XA21 .
| | Journal: J Biol . Chem . Year: 2002 | | Literature: oryza Field: abstract Doc ID: pub11927577 | Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: Moreover , the active XA21 kinase can not phosphorylate a kinase-deficient mutant of XA21 kinase .
[ Sen. 1, subscore: 1.00 ]: The rice disease resistance gene , Xa21 , encodes a receptor kinase-like protein consisting of leucine-rich repeats in the putative extracellular domain and a serine/threonine kinase in the putative intracellular domain .
[ Sen. 2, subscore: 1.00 ]: The putative XA21 kinase domain was expressed as maltose-binding and glutathione S-transferase fusion proteins in Escherichia coli .
[ Sen. 5, subscore: 1.00 ]: The relative phosphorylation rate of the XA21 kinase against increasing enzyme concentrations follows a first-order rather than second-order kinetics , indicating an intramolecular phosphorylation mechanism .
[ Sen. 7, subscore: 1.00 ]: The enzymatic activity of the XA21 kinase in a buffer containing Mn ( 2+ ) is at least 15 times higher than that with Mg ( 2+ ) .
[ Sen. 8, subscore: 1.00 ]: The K ( m ) and V ( max ) of XA21 kinase for ATP are 0 . 3 microm and 8 . 4 nmol/mg/min , respectively .
[ Sen. 9, subscore: 1.00 ]: Tryptic phosphopeptide mapping reveals that multiple sites on the XA21 kinase are phosphorylated .
[ Sen. 10, subscore: 1.00 ]: Finally , our data suggest that the region of XA21 kinase corresponding to the RD kinase activation domain is not phosphorylated , revealing a distinct mode of action compared with the tomato Pto serine/threonine kinase conferring disease resistance .
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Score: 3.00 |
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| Title: The Xanthomonas oryzae pv . lozengeoryzae raxP and raxQ genes encode an ATP sulphurylase and adenosine-5-phosphosulphate kinase that are required for AvrXa21 avirulence activity .
| | Journal: Mol . Microbiol . Year: 2002 | | Literature: oryza Field: abstract Doc ID: pub11967067 | Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: Xanthomonas oryzae pv . oryzae ( Xoo ) Philippine race 6 ( PR6 ) is unable to cause bacterial blight disease on rice lines containing the rice resistance gene Xa21 but is virulent on non-Xa21 rice lines , indicating that PR6 carries avirulence ( avrXa21 ) determinants required for recognition by XA21 .
[ Sen. 4, subscore: 1.00 ]: Xoo PR6 strains carrying disruptions in either gene , PR6DeltaraxP or PR6DeltaraxQ , are unable to produce APS and PAPS and are virulent on Xa21-containing rice lines .
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Score: 1.00 |
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| Title: [ Genome analysis of transgenic homozygous line "Minghui 63-Xa21" ] | | Journal: Sheng Wu Gong Cheng Xue Bao Year: 2002 | | Literature: oryza Field: abstract Doc ID: pub11977586 | Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: This indicated that the introduction of Xa21 into the genome of "Minghui 63" did not change these 550 genome sites and their heredity .
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Score: 4.00 |
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| Title: [ Genetic mapping of T-DNA integration sites in Xa21 transgenic rice ] | | Journal: Yi Chuan Xue Bao Year: 2002 | | Literature: oryza Field: abstract Doc ID: pub12561472 | Matching Sentences:
[ Sen. 7, subscore: 2.00 ]: The genetic mapping of T-DNA integration sites in Xa21 transgenic rice will benefit the study of position effect and stable inheritance of the transgene Xa21 .
[ Sen. 2, subscore: 1.00 ]: In the previous research we have transferred the Xa21 gene into five rice varieties of China , using Agrobacterium-mediated trasformation .
[ Sen. 3, subscore: 1.00 ]: In this study , T-DNA flanking sequences of Xa21 transgenic rice lines were obtained by using thermal asymmetric interlaced PCR ( TAIL-PCR ) .
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Score: 1.00 |
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| Title: Pyramiding transgenes for multiple resistance in rice against bacterial blight , yellow stem borer and sheath blight .
| | Journal: Theor . Appl . Genet . Year: 2002 | | Literature: oryza Field: abstract Doc ID: pub12582865 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: The Xa21 gene ( resistance to bacterial blight ) , the Bt fusion gene ( for insect resistance ) and the chitinase gene ( for tolerance of sheath blight ) were combined in a single rice line by reciprocal crossing of two transgenic homozygous IR72 lines .
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Score: 1.00 |
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| Title: Isolation and linkage analysis of expressed disease-resistance gene analogues of sugar beet ( Beta vulgaris L ) .
| | Journal: Genome Year: 2003 | | Literature: oryza Field: abstract Doc ID: pub12669798 | Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: Among the remaining RGAs , 19 revealed similarity to the serine ( threonine ) protein kinase domain of R genes , 4 showed features related to the LRR region of the rice disease resistance gene Xa21 , 1 RGA resembled the sugar beet nematode resistance gene Hs1pro-1 , and 2 had homologies to other gene products associated with disease resistance .
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Score: 1.00 |
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| Title: Cytological and molecular analysis of the Hordeum vulgare-Puccinia triticina nonhost interaction .
| | Journal: Mol .
Plant Microbe Interact .
Year: 2003 | | Literature: oryza Field: abstract Doc ID: pub12848428 | Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: Northern analysis indicated that two candidate genes , including a barley ortholog of the rice resistance gene Xa21 , are putatively involved in nonhost and non-race-specific resistance reactions .
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Score: 8.00 |
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| Title: Genetic and physical analysis of the rice bacterial blight disease resistance locus , Xa21 .
| | Journal: Mol . Gen . Genet . Year: 1992 | | Literature: oryza Field: abstract Doc ID: pub1362973 | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: One chromosome 11 marker ( RG103 ) detected polymorphism between the NILs that cosegregated with Xa21 .
[ Sen. 3, subscore: 1.00 ]: All other chromosome 11 DNA markers tested were monomorphic between the NILs , localizing the Xa21 introgressed region to an 8 . 3 cM interval on chromosome 11 .
[ Sen. 5, subscore: 1.00 ]: Genomic sequences hybridizing with RAPD818 , RAPD248 and RG103 were duplicated specifically in the Xa21 NIL .
[ Sen. 6, subscore: 1.00 ]: All three markers cosegregated with the resistance locus , Xa21 , in a F2 population of 386 progeny .
[ Sen. 7, subscore: 1.00 ]: Based on the frequency with which we recovered polymorphic Xa21-linked markers , we estimated the physical size of the introgressed region to be approximately 800 kb .
[ Sen. 8, subscore: 1.00 ]: This estimation was supported by physical mapping ( using pulsed field gel electrophoresis ) of the sequences hybridizing with the three Xa21-linked DNA markers .
[ Sen. 9, subscore: 1.00 ]: The results showed that the three Xa21-linked markers are physically close to each other , with one copy of the RAPD818 sequences located within 60 kb of RAPD248 and the other copy within 270 kb of RG103 .
[ Sen. 10, subscore: 1.00 ]: None of the enzymes tested generated a DNA fragment that hybridized with all three of the markers indicating that the introgressed region containing the resistance locus Xa21 is probably larger than 270 kb .
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Score: 4.00 |
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| Title: Cloning and characterization of receptor kinase class disease resistance gene candidates in Citrus .
| | Journal: Theor . Appl . Genet . Year: 2003 | | Literature: oryza Field: abstract Doc ID: pub13679986 | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The rice gene Xa21 represents a unique class of plant disease resistance ( R ) genes with distinct protein structure and broad-spectrum specificity ; few sequences or genes of this class have been cloned and characterized in other plant species .
[ Sen. 2, subscore: 1.00 ]: Degenerate primers were designed from the conserved motifs in the kinase domains of Xa21 and tomato Pto , and used in PCR amplification to identify this class of resistance gene candidate ( RGC ) sequences from citrus for future evaluation of possible association with citrus canker resistance .
[ Sen. 3, subscore: 1.00 ]: Twenty-nine RGC sequences highly similar to the kinase domain of Xa21 ( 55%-60% amino-acid identity ) were cloned and characterized .
[ Sen. 8, subscore: 1.00 ]: A primer-walking strategy was employed to derive full-length gene structures from two BAC clones ; both sequences 17o6RLK and 26m19RLK contained all the features of the rice Xa21 protein , including a signal peptide , the same number of leucine-rich-repeats , and transmembrane and kinase domains .
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Score: 8.00 |
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| Title: Isolation and characterization of rice mutants compromised in Xa21-mediated resistance to X oryzae pv . oryzae .
| | Journal: Theor . Appl . Genet . Year: 2004 | | Literature: oryza Field: abstract Doc ID: pub14523518 | Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: To identify genes essential for the function of the Xa21 gene , 4 , 500 IRBB21 ( Xa21 isogenic line in IR24 background ) mutants , induced by diepoxybutane and fast neutrons , were screened against Philippine race six ( PR6 ) Xoo for a change from resistance to susceptibility .
[ Sen. 1, subscore: 1.00 ]: The rice gene , Xa21 , confers resistance to diverse races of Xanthomonas oryzae pv . oryzae ( Xoo ) and encodes a receptor-like kinase with leucine-rich repeats in the extra-cellular domain .
[ Sen. 4, subscore: 1.00 ]: All fully susceptible mutants carried rearrangements at the Xa21 locus as detected by PCR and Southern hybridization .
[ Sen. 5, subscore: 1.00 ]: For the partially susceptible mutants , no changes were detected at the Xa21 locus based on Southern and PCR analyses .
[ Sen. 6, subscore: 1.00 ]: However , two of these mutants were confirmed via genetic analysis to have mutations at the Xa21 locus .
[ Sen. 7, subscore: 1.00 ]: Partially susceptible mutants exhibited variation in level of susceptibility to different Xoo strains , suggesting that they may carry different mutations required for the Xa21-mediated resistance .
[ Sen. 8, subscore: 1.00 ]: The mutants identified in this study provide useful materials for dissecting the Xa21-mediated resistance pathway in rice .
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