Score: 6.00 |
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| | Author: Sun JL Nakagawa H Karita S Ohmiya K Hattori T | | Journal: Plant Cell Physiol .
Citation: V : 37 ( 5 ) P : 612-20 Year: 1996 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub8819309 Accession (PMID): 8819309 | | Abstract: A globulin fraction prepared from rice embryos contained polypeptides or polypeptide groups of 49 kDa ( designated REG1 ) , 46 kDa ( designated REG2 ) , about 35 kDa , 32 kDa and 25 kDa .
The amino-terminal sequences of REG1 and the major polypeptide in the 35-kDa group were identical , suggesting that the REG1 polypeptide undergoes partial proteolytic processing that removes a carboxy-terminal region .
A cDNA clone , designated pcREG2 , encoding REG2 was isolated , and its nucleotide sequence was determined .
The deduced amino acid sequence of REG2 was found to be 68% identical to that of the maize GLB2 globulin .
Reg2 mRNA was present at high levels during embryo development for up to 14 days after flowering ( DAF ) .
Lower levels were found 20 DAF when the maturation of embryos was almost completed , and at the dry mature stage .
Reg2 mRNA almost disappeared upon imbibition of isolated dry mature embryos but it was re-induced at a low level by further treatment with ABA .
The expression of Reg2 was not induced by ABA in suspension-cultured cells , unlike that of Osem , one of the late embryogenesis abundant protein ( LEA ) genes . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: A globulin fraction prepared from rice embryos contained polypeptides or polypeptide groups of 49 kDa ( designated REG1 ) , 46 kDa ( designated REG2 ) , about 35 kDa , 32 kDa and 25 kDa .
[ Sen. 3, subscore: 1.00 ]: A cDNA clone , designated pcREG2 , encoding REG2 was isolated , and its nucleotide sequence was determined .
[ Sen. 4, subscore: 1.00 ]: The deduced amino acid sequence of REG2 was found to be 68% identical to that of the maize GLB2 globulin .
[ Sen. 5, subscore: 1.00 ]: Reg2 mRNA was present at high levels during embryo development for up to 14 days after flowering ( DAF ) .
[ Sen. 7, subscore: 1.00 ]: Reg2 mRNA almost disappeared upon imbibition of isolated dry mature embryos but it was re-induced at a low level by further treatment with ABA .
[ Sen. 8, subscore: 1.00 ]: The expression of Reg2 was not induced by ABA in suspension-cultured cells , unlike that of Osem , one of the late embryogenesis abundant protein ( LEA ) genes .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| | Author: Komatsu S Abbasi F Kobori E Fujisawa Y Kato H Iwasaki Y | | Journal: Proteomics Citation: V : 5 ( 15 ) P : 3932-41 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16152656 Accession (PMID): 16152656 | | Abstract: The rice dwarf1 ( d1 ) mutant , which lacks the alpha subunit of a heterotrimeric G protein ( Galpha protein ) , shows abnormal morphology due to shortened internodes , dark green leaves and grains that are small and round .
Proteome analysis was used in this study to aid in determining the function of Galpha protein in rice embryos .
Using 2-DE , seven seed embryo proteins were shown to be down-regulated in the d1 mutant as compared with its wild type .
These seven proteins included a receptor for activated C-kinase ( RACK ) and six rice embryo globulin-2 proteins ( REG2 ) .
The six REG2 have similar molecular masses with minor differences in pI .
In addition to the reduced accumulation of RACK in the d1 mutant , the increase in QL/d1 , in which a constitutively active form of the Galpha protein is expressed , was significantly higher as compared with wild type .
The level of accumulation of these seven proteins during seed development and maturation did not change significantly until the 2nd wk after pollination .
Reduced accumulation of these seven proteins started in the d1 mutant at the 3rd wk after pollination , and continued until seed maturation was complete .
All seven proteins were completely absent 24 h after imbibition in both d1 mutant and its wild type .
However , the phytohormone abscisic acid promoted the expression level of RACK after imbibition in the wild type as compared with d1 mutant .
These results suggest that RACK is regulated by Galpha-protein and plays an important role in a basic cellular process as well as in rice embryogenesis and germination . | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: These seven proteins included a receptor for activated C-kinase ( RACK ) and six rice embryo globulin-2 proteins ( REG2 ) .
[ Sen. 5, subscore: 1.00 ]: The six REG2 have similar molecular masses with minor differences in pI .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
