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| Title: Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes .
| | Author: Lee SK Song MY Seo YS Kim HK Ko S Cao PJ Suh JP Yi G Roh JH Lee S An G Hahn TR Wang GL Ronald P Jeon JS | | Journal: Genetics Citation: V : 181 P : 1627-38 Year: 2009 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub19153255 Accession (PMID): 19153255 | | Abstract: Rice blast , caused by the fungus Magnaporthe oryzae , is one of the most devastating diseases of rice .
To understand the molecular basis of Pi5-mediated resistance to M oryzae , we cloned the resistance ( R ) gene at this locus using a map-based cloning strategy .
Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50 , a susceptible rice cultivar , and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval Sequence analysis of this genomic region identified two candidate genes , Pi5-1 and Pi5-2 , which encode proteins carrying three motifs characteristic of R genes : an N-terminal coiled-coil ( CC ) motif , a nucleotide-binding ( NB ) domain , and a leucine-rich repeat ( LRR ) motif .
In genetic transformation experiments of a susceptible rice cultivar , neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to M oryzae .
In contrast , transgenic rice plants expressing both of these genes , generated by crossing transgenic lines carrying each gene individually , conferred Pi5-mediated resistance to M oryzae .
Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge , whereas the Pi5-2 gene is constitutively expressed .
These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to M oryzae .
| Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50 , a susceptible rice cultivar , and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval Sequence analysis of this genomic region identified two candidate genes , Pi5-1 and Pi5-2 , which encode proteins carrying three motifs characteristic of R genes : an N-terminal coiled-coil ( CC ) motif , a nucleotide-binding ( NB ) domain , and a leucine-rich repeat ( LRR ) motif .
[ Sen. 4, subscore: 1.00 ]: In genetic transformation experiments of a susceptible rice cultivar , neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to M oryzae .
[ Sen. 6, subscore: 1.00 ]: Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge , whereas the Pi5-2 gene is constitutively expressed .
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