|
Score: 5.00 |
|---|
| Title: Analysis of five novel putative constitutive gene promoters in transgenic rice plants .
| | Author: Park SH Yi N Kim YS Jeong MH Bang SW Choi YD Kim JK | | Journal: J Exp Bot Citation: V : 61 P : 2459-67 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20363869 Accession (PMID): 20363869 | | Abstract: Novel constitutive gene promoters are essential components of crop biotechnology .
Our analysis of five such promoters , APX , SCP1 , PGD1 , R1G1B , and EIF5 , in transgenic rice plants is reported here .
The five promoter regions were linked to the gfp reporter gene and transformed into rice .
Using fluorescent microscopy and q-RT-PCR , promoter activities were analysed in comparison with OsCc1 , Act1 , and ZmUbi1 , previously characterized as strong constitutive promoters .
The APX and PGD1 promoters direct high levels of gene expression in all it issues and stages , producing GFP at levels of up to 1 . 3% of the total soluble protein .
PGD1 is particularly active in flowers and mature roots .
The R1G1B is active in the whole grain including the embryo , endosperm , and aleurone layer , and thus represents a constitutive promoter with activity in whole seeds that has not been described previously .
The ZmUbi1 and R1G1B promoters are markedly less active in young roots and mature leaves whilst the APX , PGD1 , OsCc1 , and Act1 promoters are highly active in both vegetative and reproductive it issues .
Overall , our results demonstrate that APX , PGD1 , and R1G1B are novel gene promoters that are highly active at all stages of plant growth with distinct levels of activity .
| Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Our analysis of five such promoters , APX , SCP1 , PGD1 , R1G1B , and EIF5 , in transgenic rice plants is reported here .
[ Sen. 5, subscore: 1.00 ]: The APX and PGD1 promoters direct high levels of gene expression in all it issues and stages , producing GFP at levels of up to 1 . 3% of the total soluble protein .
[ Sen. 6, subscore: 1.00 ]: PGD1 is particularly active in flowers and mature roots .
[ Sen. 8, subscore: 1.00 ]: The ZmUbi1 and R1G1B promoters are markedly less active in young roots and mature leaves whilst the APX , PGD1 , OsCc1 , and Act1 promoters are highly active in both vegetative and reproductive it issues .
[ Sen. 9, subscore: 1.00 ]: Overall , our results demonstrate that APX , PGD1 , and R1G1B are novel gene promoters that are highly active at all stages of plant growth with distinct levels of activity .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 4.00 |
|---|
| Title: Analysis of the APX , PGD1 and R1G1B constitutive gene promoters in various organs over three homozygous generations of transgenic rice plants .
| | Author: Park SH Bang SW Jeong JS Jung H Redillas MC Kim HI Lee KH Kim YS Kim JK | | Journal: Planta Citation: V : 235 P : 1397-408 Year: 2012 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub22212906 Accession (PMID): 22212906 | | Abstract: We have previously characterized the constitutively active promoters of the APX , PGD1 and R1G1B genes in rice ( Park et al 2010 in J Exp Bot 61 : 2459-2467 ) .
To have potential crop biotechnology applications , gene promoters must be stably active over many generations .
In our current study , we report our further detailed analysis of the APX , PGD1 and R1G1B gene promoters in various organs and it issues of transgenic rice plants for three ( T ( 3 ) ( - ) ( 5 ) ) homozygous generations .
The copy numbers in 37 transgenic lines that harbor promoter : gfp constructs were determined and promoter activities were measured by real-time qPCR .
Analysis of the 37 lines revealed that 15 contained a single copy of one of the three promoter : gfp chimeric constructs .
The promoter activity levels were generally higher in multi-copy lines , whereas variations in these levels over the T ( 3 ) ( - ) ( 5 ) generations studied were observed to be smaller in single-copy than in multi-copy lines .
The three promoters were further found to be highly active in the whole plant body at both the vegetative and reproductive stages of plant growth , with the exception of the APX in the ovary and R1G1B in the pistil and filaments where zero or very low levels of activity were detected .
Of note , the spatial activities of the PGD1 promoter were found to be strikingly similar to those of the ZmUbi1 , a widely used constitutive promoter .
Our comparison of promoter activities between T ( 3 ) , T ( 4 ) and T ( 5 ) plants revealed that the APX , PGD1 and R1G1B promoters maintained their activities at comparable levels in leaves and roots over three homozygous generations and are therefore potentially viable alternative promoters for crop biotechnology applications .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: We have previously characterized the constitutively active promoters of the APX , PGD1 and R1G1B genes in rice ( Park et al 2010 in J Exp Bot 61 : 2459-2467 ) .
[ Sen. 3, subscore: 1.00 ]: In our current study , we report our further detailed analysis of the APX , PGD1 and R1G1B gene promoters in various organs and it issues of transgenic rice plants for three ( T ( 3 ) ( - ) ( 5 ) ) homozygous generations .
[ Sen. 8, subscore: 1.00 ]: Of note , the spatial activities of the PGD1 promoter were found to be strikingly similar to those of the ZmUbi1 , a widely used constitutive promoter .
[ Sen. 9, subscore: 1.00 ]: Our comparison of promoter activities between T ( 3 ) , T ( 4 ) and T ( 5 ) plants revealed that the APX , PGD1 and R1G1B promoters maintained their activities at comparable levels in leaves and roots over three homozygous generations and are therefore potentially viable alternative promoters for crop biotechnology applications .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Origin of cytoplasm substituted rice cultivars found in Japan .
| | Author: Ishikawa R Nakamura I Nishihara T Kikuchi M Senda M Akada S Harada T Niizeki M | | Journal: Citation: V : 105 ( 4 ) P : 608-613 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12582511 Accession (PMID): 12582511 | | Abstract: Genetic variation of Japanese rice cultivars were examined .
Five of 450 lowland cultivars and another five of 200 upland cultivars were determined as the indica type by using isozyme genotypes and the remainder were of the japonica type .
The major characteristics of these indica cultivars , revealed a slender shape of grains , a short apiculus hair length , a positive allele for Ph reaction , and allele-3 for the Pgd1 locus .
Three of these indica cultivars showed a non-deletion ORF100 , which is essential to the japonica-type plastid .
The plastid subtype identity ( PS-ID ) sequences of these plastids is 6C7A , which is also a japonica-specific repeat unit .
Thus , these cultivars were concluded to be naturally generated cytoplasm substituted lines .
These plastids were introduced into a indica genetic background from japonica cultivars grown elsewhere .
The rest of the indica cultivars revealed a deletion-type ORF100 and plastid subtype 8C8A , both of which are indica-specific .
These cultivars carried indica-type allelic constitutions for diagnostic isozyme loci .
However , other characters were identical to the cytoplasm-substituted cultivars in Japan .
In East and Southeast Asia , cultivars carrying a indica-type nuclear genotype with a japonica-type plastid are restricted to Aus cultivars in the Bengal region .
Genetic and historical records suggest that Japanese indica cultivars and the Aus cultivars are closely related .
The Aus cultivars acquire necessary genetic constitutions from both indica and japonica cultivars through naturally occurring out-crossing to adapt to a particular cultivation condition in the region .
The wide adaptability enabled them to be introduced into a northern region like Japan .
| Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: The major characteristics of these indica cultivars , revealed a slender shape of grains , a short apiculus hair length , a positive allele for Ph reaction , and allele-3 for the Pgd1 locus .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Analysis of the APX , PGD1 and R1G1B constitutive gene promoters in various organs over three homozygous generations of transgenic rice plants .
| | Author: Park SH Bang SW Jeong JS Jung H Redillas MC Kim HI Lee KH Kim YS Kim JK | | Journal: Planta Citation: V : 235 P : 1397-408 Year: 2012 Type: In-Process | | Literature: oryza Field: title Doc ID: pub22212906 Accession (PMID): 22212906 | | Abstract: We have previously characterized the constitutively active promoters of the APX , PGD1 and R1G1B genes in rice ( Park et al 2010 in J Exp Bot 61 : 2459-2467 ) .
To have potential crop biotechnology applications , gene promoters must be stably active over many generations .
In our current study , we report our further detailed analysis of the APX , PGD1 and R1G1B gene promoters in various organs and it issues of transgenic rice plants for three ( T ( 3 ) ( - ) ( 5 ) ) homozygous generations .
The copy numbers in 37 transgenic lines that harbor promoter : gfp constructs were determined and promoter activities were measured by real-time qPCR .
Analysis of the 37 lines revealed that 15 contained a single copy of one of the three promoter : gfp chimeric constructs .
The promoter activity levels were generally higher in multi-copy lines , whereas variations in these levels over the T ( 3 ) ( - ) ( 5 ) generations studied were observed to be smaller in single-copy than in multi-copy lines .
The three promoters were further found to be highly active in the whole plant body at both the vegetative and reproductive stages of plant growth , with the exception of the APX in the ovary and R1G1B in the pistil and filaments where zero or very low levels of activity were detected .
Of note , the spatial activities of the PGD1 promoter were found to be strikingly similar to those of the ZmUbi1 , a widely used constitutive promoter .
Our comparison of promoter activities between T ( 3 ) , T ( 4 ) and T ( 5 ) plants revealed that the APX , PGD1 and R1G1B promoters maintained their activities at comparable levels in leaves and roots over three homozygous generations and are therefore potentially viable alternative promoters for crop biotechnology applications .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Analysis of the APX , PGD1 and R1G1B constitutive gene promoters in various organs over three homozygous generations of transgenic rice plants .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
