Score: 1.00 | | Author: de Carvalho Lima KG Takahashi CM Alterthum F | Journal: J Ind . Microbiol . Biotechnol . Citation: V : 29 ( 3 ) P : 124-8 Year: 2002 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub12242633 Accession (PMID): 12242633 | Abstract: Corn cob hydrolysates , with xylose as the dominant sugar , were fermented to ethanol by recombinant Escherichia coli KO11 .
When inoculum was grown on LB medium containing glucose , fermentation of the hydrolysate was completed in 163 h and ethanol yield was 0 . 50 g ethanol/g sugar .
When inoculum was grown on xylose , ethanol yield dropped , but fermentation was faster ( 113 h ) .
Hydrolysate containing 72 . 0 g/l xylose and supplemented with 20 . 0 g/l rice bran was readily fermented , producing 36 . 0 g/l ethanol within 70 h .
Maximum ethanol concentrations were not higher for fermentations using higher cellular concentration inocula .
A simulation of an industrial process integrating pentose fermentation by E coli and hexose fermentation by yeast was carried out .
At the first step , E coli fermented the hydrolysate containing 85 . 0 g/l xylose , producing 40 . 0 g/l ethanol in 94 h .
Bakers yeast and sucrose ( 150 . 0 g/l ) were then added to the spent fermentation broth .
After 8 h of yeast fermentation , the ethanol concentration reached 104 . 0 g/l .
This two-stage fermentation can render the bioconversion of lignocellulose to ethanol more attractive due to increased final alcohol concentration .
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Score: 1.00 | | Author: de Carvalho Lima KG Takahashi CM Alterthum F | Journal: J Ind . Microbiol . Biotechnol . Citation: V : 29 ( 3 ) P : 124-8 Year: 2002 Type: ARTICLE | Literature: oryza Field: title Doc ID: pub12242633 Accession (PMID): 12242633 | Abstract: Corn cob hydrolysates , with xylose as the dominant sugar , were fermented to ethanol by recombinant Escherichia coli KO11 .
When inoculum was grown on LB medium containing glucose , fermentation of the hydrolysate was completed in 163 h and ethanol yield was 0 . 50 g ethanol/g sugar .
When inoculum was grown on xylose , ethanol yield dropped , but fermentation was faster ( 113 h ) .
Hydrolysate containing 72 . 0 g/l xylose and supplemented with 20 . 0 g/l rice bran was readily fermented , producing 36 . 0 g/l ethanol within 70 h .
Maximum ethanol concentrations were not higher for fermentations using higher cellular concentration inocula .
A simulation of an industrial process integrating pentose fermentation by E coli and hexose fermentation by yeast was carried out .
At the first step , E coli fermented the hydrolysate containing 85 . 0 g/l xylose , producing 40 . 0 g/l ethanol in 94 h .
Bakers yeast and sucrose ( 150 . 0 g/l ) were then added to the spent fermentation broth .
After 8 h of yeast fermentation , the ethanol concentration reached 104 . 0 g/l .
This two-stage fermentation can render the bioconversion of lignocellulose to ethanol more attractive due to increased final alcohol concentration .
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