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| Title: Mutation of the chitinase-like protein-encoding AtCTL2 gene enhances lignin accumulation in dark-grown Arabidopsis seedlings .
| | Author: Hossain MA Noh HN Kim KI Koh EJ Wi SG Bae HJ Lee H Hong SW | | Journal: J Plant Physiol Citation: V : 167 P : 650-8 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20056293 Accession (PMID): 20056293 | | Abstract: Several genes that encode a chitinase-like protein ( called the CTL group ) have been identified in Arabidopsis , rice , pea , and cotton .
Members of the CTL group have attracted much attention because of their possible role in the biosynthesis of the cell wall in plants .
The hot2 mutation in the CTL1 ( AtCTL1 ) gene of Arabidopsis thaliana causes multiple defects in growth and development .
The Arabidopsis genome possesses the AtCTL2 gene , which exhibits 70% similarity to AtCTL1 at the amino acid level .
We showed that the AtCTL2 gene was predominantly expressed in stems , which was in contrast to the presence of AtCTL1 transcripts in most organs of Arabidopsis .
In addition , beta-glucuronidase ( GUS ) staining was detectable in all it issues of the stem in transgenic plants expressing the AtCTL1 : : GUS construct , while GUS activity under control of the AtCTL2 promoter was significantly restricted to the xylem and to interfascicular fibers in stems .
The phenotypes of atctl2 single mutant and of hot2 , atctl2 double mutant plants were significantly similar to those of wild-type and of hot2 single mutant plants , respectively .
The expression levels of CESA1 and CESA4 transcripts were not affected in the two single mutants or corresponding double mutant plants , compared with the levels in wild-type plants .
The accumulation of lignin in etiolated hypocotyls , however , was increased by mutation of AtCTL2 .
These findings suggest that AtCTL2 is required for proper cell wall biosynthesis in etiolated seedlings of Arabidopsis .
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[ Sen. 8, subscore: 1.00 ]: The expression levels of CESA1 and CESA4 transcripts were not affected in the two single mutants or corresponding double mutant plants , compared with the levels in wild-type plants .
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