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Score: 1.00 |
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| Title: Trehalose synthesis and metabolism are required at different stages of plant infection by Magnaporthe grisea .
| | Author: Foster AJ Jenkinson JM Talbot NJ .
| | Journal: EMBO J Citation: V : 22 ( 2 ) P : 225-35 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12514128 Accession (PMID): 12514128 | | Abstract: The relationship of trehalose metabolism to fungal virulence was explored in the rice blast fungus Magnaporthe grisea .
To determine the role of trehalose synthesis in pathogenesis , we identified and deleted TPS1 , encoding trehalose-6-phosphate synthase .
A Deltatps1 mutant failed to synthesize trehalose , sporulated poorly and was greatly attenuated in pathogenicity .
Appressoria produced by Deltatps1 did not develop full turgor or elaborate penetration hyphae efficiently .
To determine the role of subsequent trehalose breakdown , we deleted NTH1 , which encodes a neutral trehalase .
Nth1 mutants infected plants normally , but showed attenuated pathogenicity due to a decreased ability to colonize plant it issue .
A second trehalase was also identified , required both for growth on trehalose and mobilization of intracellular trehalose during infection-related development .
TRE1 encodes a cell wall-localized enzyme with characteristics of both neutral and acidic trehalases , but is dispensable for pathogenicity .
Our results indicate that trehalose synthesis , but not its subsequent breakdown , is required for primary plant infection by Mgrisea , while trehalose degradation is important for efficient development of the fungus in plant it issue following initial infection . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: To determine the role of trehalose synthesis in pathogenesis , we identified and deleted TPS1 , encoding trehalose-6-phosphate synthase .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 4.00 |
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| Title: An NADPH-dependent genetic switch regulates plant infection by the rice blast fungus .
| | Author: Wilson RA Gibson RP Quispe CF Littlechild JA Talbot NJ | | Journal: Proc Natl Acad Sci U S A Citation: V : 107 P : 21902-7 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21115813 Accession (PMID): 21115813 | | Abstract: To cause rice blast disease , the fungus Magnaporthe oryzae breaches the tough outer cuticle of the rice leaf by using specialized infection structures called appressoria .
These cells allow the fungus to invade the host plant and proliferate rapidly within leaf it issue .
Here , we show that a unique NADPH-dependent genetic switch regulates plant infection in response to the changing nutritional and redox conditions encountered by the pathogen .
The biosynthetic enzyme trehalose-6-phosphate synthase ( Tps1 ) integrates control of glucose-6-phosphate metabolism and nitrogen source utilization by regulating the oxidative pentose phosphate pathway , the generation of NADPH , and the activity of nitrate reductase .
We report that Tps1 directly binds to NADPH and , thereby , regulates a set of related transcriptional corepressors , comprising three proteins , Nmr1 , Nmr2 , and Nmr3 , which can each bind NADP .
Targeted deletion of any of the Nmr-encoding genes partially suppresses the nonpathogenic phenotype of a Deltatps1 mutant .
Tps1-dependent Nmr corepressors control the expression of a set of virulence-associated genes that are derepressed during appressorium-mediated plant infection .
When considered together , these results suggest that initiation of rice blast disease by M oryzae requires a regulatory mechanism involving an NADPH sensor protein , Tps1 , a set of NADP-dependent transcriptional corepressors , and the nonconsuming interconversion of NADPH and NADP acting as signal transducer .
| Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: The biosynthetic enzyme trehalose-6-phosphate synthase ( Tps1 ) integrates control of glucose-6-phosphate metabolism and nitrogen source utilization by regulating the oxidative pentose phosphate pathway , the generation of NADPH , and the activity of nitrate reductase .
[ Sen. 5, subscore: 1.00 ]: We report that Tps1 directly binds to NADPH and , thereby , regulates a set of related transcriptional corepressors , comprising three proteins , Nmr1 , Nmr2 , and Nmr3 , which can each bind NADP .
[ Sen. 7, subscore: 1.00 ]: Tps1-dependent Nmr corepressors control the expression of a set of virulence-associated genes that are derepressed during appressorium-mediated plant infection .
[ Sen. 8, subscore: 1.00 ]: When considered together , these results suggest that initiation of rice blast disease by M oryzae requires a regulatory mechanism involving an NADPH sensor protein , Tps1 , a set of NADP-dependent transcriptional corepressors , and the nonconsuming interconversion of NADPH and NADP acting as signal transducer .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Analysis of trehalose-6-phosphate synthase ( TPS ) gene family suggests the formation of TPS complexes in rice .
| | Author: Zang B Li H Li W Deng XW Wang X | | Journal: Plant Mol Biol Citation: V : 76 P : 507-22 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21598083 Accession (PMID): 21598083 | | Abstract: Trehalose-6-phosphate ( T6P ) , an intermediate in the trehalose biosynthesis pathway , is emerging as an important regulator of plant metabolism and development .
T6P levels are potentially modulated by a group of trehalose-6-phosphate synthase ( TPS ) and trehalose-6-phosphate phosphatase ( TPP ) homologues .
In this study , we have isolated 11 TPS genes encoding proteins with both TPS and TPP domains , from rice .
Functional complement assays performed in yeast tps1 and tps2 mutants , revealed that only OsTPS1 encodes an active TPS enzyme and no OsTPS protein possesses TPP activity .
By using a yeast two-hybrid analysis , a complicated interaction network occurred among OsTPS proteins , and the TPS domain might be essential for this interaction to occur .
The interaction between OsTPS1 and OsTPS8 in vivo was confirmed by bimolecular fluorescence complementation and coimmunoprecipitation assays .
Furthermore , our gel filtration assay showed that there may exist two forms of OsTPS1 ( OsTPS1a and OsTPS1b ) with different elution profiles in rice .
OsTPS1b was particularly cofractionated with OsTPS5 and OsTPS8 in the 360 kDa complex , while OsTPS1a was predominantly incorporated into the complexes larger than 360 kDa .
Collectively , these results suggest that OsTPS family members may form trehalose-6-phosphate synthase complexes and therefore potentially modify T6P levels to regulate plant development .
| Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: Functional complement assays performed in yeast tps1 and tps2 mutants , revealed that only OsTPS1 encodes an active TPS enzyme and no OsTPS protein possesses TPP activity .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
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| Title: Principles of carbon catabolite repression in the rice blast fungus : Tps1 , Nmr1-3 , and a MATE-family pump regulate glucose metabolism during infection .
| | Author: Fernandez J Wright JD Hartline D Quispe CF Madayiputhiya N Wilson RA | | Journal: PLoS Genet Citation: V : 8 P : e1002673 Year: 2012 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub22570632 Accession (PMID): 22570632 | | Abstract: Understanding the genetic pathways that regulate how pathogenic fungi respond to their environment is paramount to developing effective mitigation strategies against disease .
Carbon catabolite repression ( CCR ) is a global regulatory mechanism found in a wide range of microbial organisms that ensures the preferential utilization of glucose over less favourable carbon sources , but little is known about the components of CCR in filamentous fungi .
Here we report three new mediators of CCR in the devastating rice blast fungus Magnaporthe oryzae : the sugar sensor Tps1 , the Nmr1-3 inhibitor proteins , and the multidrug and toxin extrusion ( MATE ) -family pump , Mdt1 .
Using simple plate tests coupled with transcriptional analysis , we show that Tps1 , in response to glucose-6-phosphate sensing , triggers CCR via the inactivation of Nmr1-3 .
In addition , by dissecting the CCR pathway using Agrobacterium tumefaciens-mediated mutagenesis , we also show that Mdt1 is an additional and previously unknown regulator of glucose metabolism .
Mdt1 regulates glucose assimilation downstream of Tps1 and is necessary for nutrient utilization , sporulation , and pathogenicity .
This is the first functional characterization of a MATE-family protein in filamentous fungi and the first description of a MATE protein in genetic regulation or plant pathogenicity .
Perturbing CCR in Deltatps1 and MDT1 disruption strains thus results in physiological defects that impact pathogenesis , possibly through the early expression of cell wall-degrading enzymes .
Taken together , the importance of discovering three new regulators of carbon metabolism lies in understanding how M oryzae and other pathogenic fungi respond to nutrient availability and control development during infection .
| Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: Here we report three new mediators of CCR in the devastating rice blast fungus Magnaporthe oryzae : the sugar sensor Tps1 , the Nmr1-3 inhibitor proteins , and the multidrug and toxin extrusion ( MATE ) -family pump , Mdt1 .
[ Sen. 4, subscore: 1.00 ]: Using simple plate tests coupled with transcriptional analysis , we show that Tps1 , in response to glucose-6-phosphate sensing , triggers CCR via the inactivation of Nmr1-3 .
[ Sen. 6, subscore: 1.00 ]: Mdt1 regulates glucose assimilation downstream of Tps1 and is necessary for nutrient utilization , sporulation , and pathogenicity .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Principles of carbon catabolite repression in the rice blast fungus : Tps1 , Nmr1-3 , and a MATE-family pump regulate glucose metabolism during infection .
| | Author: Fernandez J Wright JD Hartline D Quispe CF Madayiputhiya N Wilson RA | | Journal: PLoS Genet Citation: V : 8 P : e1002673 Year: 2012 Type: In-Process | | Literature: oryza Field: title Doc ID: pub22570632 Accession (PMID): 22570632 | | Abstract: Understanding the genetic pathways that regulate how pathogenic fungi respond to their environment is paramount to developing effective mitigation strategies against disease .
Carbon catabolite repression ( CCR ) is a global regulatory mechanism found in a wide range of microbial organisms that ensures the preferential utilization of glucose over less favourable carbon sources , but little is known about the components of CCR in filamentous fungi .
Here we report three new mediators of CCR in the devastating rice blast fungus Magnaporthe oryzae : the sugar sensor Tps1 , the Nmr1-3 inhibitor proteins , and the multidrug and toxin extrusion ( MATE ) -family pump , Mdt1 .
Using simple plate tests coupled with transcriptional analysis , we show that Tps1 , in response to glucose-6-phosphate sensing , triggers CCR via the inactivation of Nmr1-3 .
In addition , by dissecting the CCR pathway using Agrobacterium tumefaciens-mediated mutagenesis , we also show that Mdt1 is an additional and previously unknown regulator of glucose metabolism .
Mdt1 regulates glucose assimilation downstream of Tps1 and is necessary for nutrient utilization , sporulation , and pathogenicity .
This is the first functional characterization of a MATE-family protein in filamentous fungi and the first description of a MATE protein in genetic regulation or plant pathogenicity .
Perturbing CCR in Deltatps1 and MDT1 disruption strains thus results in physiological defects that impact pathogenesis , possibly through the early expression of cell wall-degrading enzymes .
Taken together , the importance of discovering three new regulators of carbon metabolism lies in understanding how M oryzae and other pathogenic fungi respond to nutrient availability and control development during infection .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Principles of carbon catabolite repression in the rice blast fungus : Tps1 , Nmr1-3 , and a MATE-family pump regulate glucose metabolism during infection .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
