Untitled Document


Query
Condition	Concatenation	Type	Data Entry	Comparison	Numerics	Sentence Range	Exact Match?	Case Sensitive?	Literatures	Fields
0	&&	keyword	AIP2	>	0	sentence	no	no	oryza	body, title, abstract

4 matches found in 2 documents. Results sorted by .


Score: 2.00
Title: A RING finger E3 ligase gene , Oryza sativa Delayed Seed Germination 1 ( OsDSG1 ) , controls seed germination and stress responses in rice .
Author: Park GG Park JJ Yoon J Yu SN An G
Journal: Plant Mol Biol Citation: V : 74 P : 467-78 Year: 2010 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub20878348 Accession (PMID): 20878348
Abstract: Seed germination is an important character for plant growth and seed quality . We identified a rice mutant that was delayed in its germination . There , T-DNA was inserted into Oryza sativa Delayed Seed Germination 1 ( OsDSG1 ) , causing a recessive null mutation . Overexpression of the gene enhanced seed germination . OsDSG1 is most similar to Arabidopsis AIP2 , an E3 ligase targeting ABI3 . Yeast two-hybrid experiments showed that our OsDSG1 binds to OsABI3 , indicating that OsDSG1 is a rice ortholog of AIP2 . Self-ubiquitination assay indicated that bacterially expressed OsDSG1 protein has E3 ubiquitin ligase activity . Real-time PCR analysis revealed that OsDSG1 was expressed in leaves and roots , and strongly in developing seeds . In addition to the delayed-germination phenotype , mutant plants were shorter and had greater tolerance to high-salt and drought stresses . In the osdsg1 mutant , transcript levels of ABA signaling genes and ABA responsive genes were significantly increased . By contrast , expressions of OsGAMYB and its downstream genes that encode hydrolytic enzymes were markedly reduced . These observations support that OsDSG1 is a major regulator of ABA signaling in germinating seeds . Finally , we observed that the germination rates of various rice cultivars depended upon the transcript levels of OsDSG1 and other ABA-signaling genes .
Matching Sentences: [ Sen. 5, subscore: 2.00 ]: OsDSG1 is most similar to Arabidopsis AIP2 , an E3 ligase targeting ABI3 . Yeast two-hybrid experiments showed that our OsDSG1 binds to OsABI3 , indicating that OsDSG1 is a rice ortholog of AIP2 .
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Score: 3.00
Title: Rice APOPTOSIS INHIBITOR5 coupled with two DEAD-box adenosine 5-triphosphate-dependent RNA helicases regulates tapetum degeneration .
Author: Li X Gao X Wei Y Deng L Ouyang Y Chen G Li X Zhang Q Wu C
Journal: Plant Cell Citation: V : 23 P : 1416-34 Year: 2011 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub21467577 Accession (PMID): 21467577
Abstract: Programmed cell death ( PCD ) during tapetum degeneration in postmeiotic anthers is critical for the proper development of male gametophytes in flowering plants . Although several genes involved in this process have been identified recently , the molecular mechanism is still poorly understood . Here , we show that knockout of rice ( Oryza sativa ) APOPTOSIS INHIBITOR5 ( API5 ) , which encodes a putative homolog of antiapoptosis protein Api5 in animals , results in delayed degeneration of the tapetum due to inhibition of the tapetal PCD process leading to defects in formation of male gametophytes . Os API5 is a nuclear protein that interacts with two DEAD-box ATP-dependent RNA helicases , API5-INTERACTING PROTEIN1 ( AIP1 ) and AIP2 . AIP1 and AIP2 are homologs of yeast ( Saccharomyces cerevisiae ) Suppressor of Bad Response to Refrigeration1 protein 2 ( SUB2p ) that have critical roles in transcription elongation and pre-mRNA splicing . Os AIP1 and AIP2 can form dimers and interact directly with the promoter region of CP1 , a rice cysteine protease gene . Suppression of Os AIP1/2 leads to down-regulation of CP1 , resulting in sterility , which is highly similar to the effects of suppressed expression of Os CP1 . Our results uncover a previously unknown pathway for regulating PCD during tapetum degeneration in rice , one that may be conserved among eukaryotic organisms .
Matching Sentences: [ Sen. 4, subscore: 1.00 ]: Os API5 is a nuclear protein that interacts with two DEAD-box ATP-dependent RNA helicases , API5-INTERACTING PROTEIN1 ( AIP1 ) and AIP2 . [ Sen. 5, subscore: 1.00 ]: AIP1 and AIP2 are homologs of yeast ( Saccharomyces cerevisiae ) Suppressor of Bad Response to Refrigeration1 protein 2 ( SUB2p ) that have critical roles in transcription elongation and pre-mRNA splicing . [ Sen. 6, subscore: 1.00 ]: Os AIP1 and AIP2 can form dimers and interact directly with the promoter region of CP1 , a rice cysteine protease gene .
Supplemental links/files: reference in endnote online text related articles pubmed citation