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| Title: Cloning of nicotianamine synthase genes , novel genes involved in the biosynthesis of phytosiderophores .
| | Author: Higuchi K Suzuki K Nakanishi H Yamaguchi H Nishizawa NK Mori S | | Journal: Plant Physiol .
Citation: V : 119 ( 2 ) P : 471-80 Year: 1999 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub9952442 Accession (PMID): 9952442 | | Abstract: Nicotianamine synthase ( NAS ) , the key enzyme in the biosynthetic pathway for the mugineic acid family of phytosiderophores , catalyzes the trimerization of S-adenosylmethionine to form one molecule of nicotianamine .
We purified NAS protein and isolated the genes nas1 , nas2 , nas3 , nas4 , nas5-1 , nas5-2 , and nas6 , which encode NAS and NAS-like proteins from Fe-deficient barley ( Hordeum vulgare L cv Ehimehadaka no 1 ) roots .
Escherichia coli expressing nas1 showed NAS activity , confirming that this gene encodes a functional NAS .
Expression of nas genes as determined by northern-blot analysis was induced by Fe deficiency and was root specific .
The NAS genes form a multigene family in the barley and rice genomes . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: We purified NAS protein and isolated the genes nas1 , nas2 , nas3 , nas4 , nas5-1 , nas5-2 , and nas6 , which encode NAS and NAS-like proteins from Fe-deficient barley ( Hordeum vulgare L cv Ehimehadaka no 1 ) roots .
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