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Score: 1.00 |
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| Title: [ The construction and screening of genomic library based on transformable artificial chromosome ( TAC ) vector in Lotus japonicus ] | | Author: Guo XZ Liu YG Luo D | | Journal: Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao Citation: V : 30 ( 2 ) P : 234-8 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15599054 Accession (PMID): 15599054 | | Abstract: Many vectors , especially artificial chromosome vectors , have been developed for genome-scale mapping and sequencing .
As the first artificial chromosome , YAC has been extensively used in the genomic library construction and mapping .
Shizuya et al ( 1992 ) devised the bacterial artificial chromosome ( BAC ) originated from F factor of Ecoli , which is much easy to handle and isolated with large harboring capacity .
Liu et al ( 1999 ) designed the transformation-competent artificial chromosome ( TAC ) vector , derived from PAC vector .
TAC could shuttle a large-scale DNA fragment between bacteria and Agrobaceria .
Further , it could integrate a large targetted DNA fragment into plant genome , as being documented in Arabidopsis and rice genome research ( Liu et al 2000 , 2002 ) .
The time-saving virtue of TAC should be significant in genomics research in Lotus japonicus , a model plant of legume .
Using a nuclei-based method of Liu and Whittier , high molecular weight DNA was isolated from Lotus japonicus ( Gifu ecotype ) .
The DNA was digested partially with Hind III and size-fractionated in the 10 to 20-kb size range as described ( Liu and Whittier 1994 ) .
The partially digested and size selected DNA fragments were ligated with Hind III-digested pYLTAC7 and then used for transformation of E coli DH10B by electroporation .
Transformants carrying inserts were selected on LB agar plates containing 25 mg/L kanamycin and 5% sucrose .
The library , 6 haploid genome equivalents , was pooled in 12 96-well microtiter plates at about 150 transformants per well .
The TAC library was then arrayed in nylon membranes and subjected to screening .
The probe , a homolog fragment of CEN gene controlling the structure of inflorescence Antirrhinum , was used for screening .
0 . 5 muL solution from the positive pool was titered and the secondary screening was conducted in the same way .
Finally , these positive clonies were confirmed by Southern blot .
These data showed that this genomic library was reliable for further molecular research in Lotus japonicus .
| Matching Sentences:
[ Sen. 10, subscore: 1.00 ]: The partially digested and size selected DNA fragments were ligated with Hind III-digested pYLTAC7 and then used for transformation of E coli DH10B by electroporation .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 5.00 |
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| Title: [ Analysis and mapping of a rice repeated sequence ] | | Author: Jiang L Zheng XW Zhang XH Han JM Dong JG Zhai WX .
| | Journal: Yi Chuan Citation: V : 25 ( 6 ) P : 691-4 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15639961 Accession (PMID): 15639961 | | Abstract: A repeated sequence with a length of 560 bp , termed as DH17 , was obtained during PCR amplification of rice NBS-LRR homologues .
A repeated unit of 352 bp in the DH17 fragment was revealed through sequence analysis and comparison , which has a high homology with the known sequences of OS48 and TrsA , and belongs to the same repeat family .
Southern hybridization displayed that there are higher DH17 copies in the genome of an indica variety , ZYQ8 , than that in the genome of japonica variety , JX17 .
The tandom repeated DH17 sequence was mapped on the long arm end of chromosome 12 through RFLP analysis of a double haploid population derived from ZYQ8 and JX17 using DH17 as a probe .
| Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: The tandom repeated DH17 sequence was mapped on the long arm end of chromosome 12 through RFLP analysis of a double haploid population derived from ZYQ8 and JX17 using DH17 as a probe .
[ Sen. 1, subscore: 1.00 ]: A repeated sequence with a length of 560 bp , termed as DH17 , was obtained during PCR amplification of rice NBS-LRR homologues .
[ Sen. 2, subscore: 1.00 ]: A repeated unit of 352 bp in the DH17 fragment was revealed through sequence analysis and comparison , which has a high homology with the known sequences of OS48 and TrsA , and belongs to the same repeat family .
[ Sen. 3, subscore: 1.00 ]: Southern hybridization displayed that there are higher DH17 copies in the genome of an indica variety , ZYQ8 , than that in the genome of japonica variety , JX17 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 10.00 |
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| Title: DH1 , a LOB domain-like protein required for glume formation in rice .
| | Author: Li A Zhang Y Wu X Tang W Wu R Dai Z Liu G Zhang H Wu C Chen G Pan X | | Journal: Plant Mol Biol Citation: V : 66 P : 491-502 Year: 2008 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub18180880 Accession (PMID): 18180880 | | Abstract: T-DNA tagging is a high throughput strategy for identifying and cloning functional genes in plants .
In this study , we screened 4416 lab-created T ( 1 ) rice T-DNA tagged lines and identified a mutant , designated dh1 ( degenerated hull1 ) , with phenotype of degenerated hull and naked pistils and stamens .
Approximately 60% florets on the dh1 panicle defected in forming normal palea and lemma .
Instead , they formed degenerative velum-like or filamentous organs accompanying with the lack of lodicules , stamens and pistils at different degree .
A 361 bp of genomic sequence flanking the T-DNA isolated using TAIL-PCR ( Thermal asymmetric interlaced PCR ) co-segregated with the mutation phenotype .
Results of blastn and gene prediction revealed the T-DNA inserted into the promoter region of a function-predicted gene at 283 bp upstream of its transcription start site ( TSS ) .
The predicted gene encoded a LOB ( Lateral Organ Boundaries ) domain-like protein .
RT-PCR analyses indicated the transcription level of target candidate gene , DH1 , decreased significantly in dh1 mutant .
RNAi aimed at DH1 in wild type plants could partially result in the mutation phenotype of dh1 .
DH1 could also rescue the mutation phenotype in the complement experiment .
The result of transformation by a fused expression vector , pDH1 : : GFP , revealed that DH1 had the keen spatial and temporal characteristics of expressing at axillary bud , young panicle and floral organs but not at root , leaf , node and culm , and strongly expressing at young it issues but weakly at mature organs .
The dh1 presented severer mutation phenotype under relatively longer daylight than under shorter daylight implied that shorter daylight induced the expression of gene ( s ) redundant to DH1 in function and partially compensated for the loss-of-function .
It is the first time to report the LOB-domain gene participating in the development of floral organs in rice .
| Matching Sentences:
[ Sen. 8, subscore: 2.00 ]: RT-PCR analyses indicated the transcription level of target candidate gene , DH1 , decreased significantly in dh1 mutant .
[ Sen. 9, subscore: 2.00 ]: RNAi aimed at DH1 in wild type plants could partially result in the mutation phenotype of dh1 .
[ Sen. 12, subscore: 2.00 ]: The dh1 presented severer mutation phenotype under relatively longer daylight than under shorter daylight implied that shorter daylight induced the expression of gene ( s ) redundant to DH1 in function and partially compensated for the loss-of-function .
[ Sen. 2, subscore: 1.00 ]: In this study , we screened 4416 lab-created T ( 1 ) rice T-DNA tagged lines and identified a mutant , designated dh1 ( degenerated hull1 ) , with phenotype of degenerated hull and naked pistils and stamens .
[ Sen. 3, subscore: 1.00 ]: Approximately 60% florets on the dh1 panicle defected in forming normal palea and lemma .
[ Sen. 10, subscore: 1.00 ]: DH1 could also rescue the mutation phenotype in the complement experiment .
[ Sen. 11, subscore: 1.00 ]: The result of transformation by a fused expression vector , pDH1 : : GFP , revealed that DH1 had the keen spatial and temporal characteristics of expressing at axillary bud , young panicle and floral organs but not at root , leaf , node and culm , and strongly expressing at young it issues but weakly at mature organs .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Expression of rice gall dwarf virus outer coat protein gene ( S8 ) in insect cells .
| | Author: Fan GC Gao FL Wei TY Huang MY Xie LY Wu ZJ Lin QY Xie LH | | Journal: Virol Sin Citation: V : 25 P : 401-8 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21221918 Accession (PMID): 21221918 | | Abstract: To obtain the P8 protein of Rice gall dwarf virus ( RGDV ) with biological activity , its outer coat protein gene S8 was expressed in Spodoptera frugiperda ( Sf9 ) insect cells using the baculovirus expression system .
The S8 gene was subcloned into the pFastBac1 vector , to produce the recombinant baculovirus transfer vector pFB-S8 .
After transformation , pFB-S8 was introduced into the competent cells ( E coli DH10Bac ) containing a shuttle vector , Bacmid , generating the recombinant bacmid rbpFB-S8 .
After being infected by recombinant baculovirus rvpFB-S8 at different multiplicities of infection , Sf9 cells were collected at different times and analyzed by SDS-PAGE , Western blotting and immunofluorescence microscopy .
The expression level of the P8 protein was highest between 48-72 h after transfection of Sf9 cells .
Immunofluorescence microscopy showed that P8 protein of RGDV formed punctate structures in the cytoplasm of Sf9 cells .
| Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: After transformation , pFB-S8 was introduced into the competent cells ( E coli DH10Bac ) containing a shuttle vector , Bacmid , generating the recombinant bacmid rbpFB-S8 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: DH1 , a LOB domain-like protein required for glume formation in rice .
| | Author: Li A Zhang Y Wu X Tang W Wu R Dai Z Liu G Zhang H Wu C Chen G Pan X | | Journal: Plant Mol Biol Citation: V : 66 P : 491-502 Year: 2008 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub18180880 Accession (PMID): 18180880 | | Abstract: T-DNA tagging is a high throughput strategy for identifying and cloning functional genes in plants .
In this study , we screened 4416 lab-created T ( 1 ) rice T-DNA tagged lines and identified a mutant , designated dh1 ( degenerated hull1 ) , with phenotype of degenerated hull and naked pistils and stamens .
Approximately 60% florets on the dh1 panicle defected in forming normal palea and lemma .
Instead , they formed degenerative velum-like or filamentous organs accompanying with the lack of lodicules , stamens and pistils at different degree .
A 361 bp of genomic sequence flanking the T-DNA isolated using TAIL-PCR ( Thermal asymmetric interlaced PCR ) co-segregated with the mutation phenotype .
Results of blastn and gene prediction revealed the T-DNA inserted into the promoter region of a function-predicted gene at 283 bp upstream of its transcription start site ( TSS ) .
The predicted gene encoded a LOB ( Lateral Organ Boundaries ) domain-like protein .
RT-PCR analyses indicated the transcription level of target candidate gene , DH1 , decreased significantly in dh1 mutant .
RNAi aimed at DH1 in wild type plants could partially result in the mutation phenotype of dh1 .
DH1 could also rescue the mutation phenotype in the complement experiment .
The result of transformation by a fused expression vector , pDH1 : : GFP , revealed that DH1 had the keen spatial and temporal characteristics of expressing at axillary bud , young panicle and floral organs but not at root , leaf , node and culm , and strongly expressing at young it issues but weakly at mature organs .
The dh1 presented severer mutation phenotype under relatively longer daylight than under shorter daylight implied that shorter daylight induced the expression of gene ( s ) redundant to DH1 in function and partially compensated for the loss-of-function .
It is the first time to report the LOB-domain gene participating in the development of floral organs in rice .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: DH1 , a LOB domain-like protein required for glume formation in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
