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| Title: Novel miRNAs in the control of arsenite levels in rice .
| | Author: Liu Q | | Journal: Funct Integr Genomics Citation: V : P : Year: 2012 Type: Publisher | | Literature: oryza Field: abstract Doc ID: pub22585409 Accession (PMID): 22585409 | | Abstract: In a species , the miRNA repertoire comprises many lineage or species-specific miRNAs .
Using deep sequencing , the whole transcriptome of seedling roots was studied and a total of 18 novel miRNAs in indica rice Minghui 86 were identified .
Among these novel miRNAs six were up-regulated and two were down-regulated under arsenite stress .
Quantitative real-time RT-PCR analysis revealed that miR6254 was predominantly expressed in roots relative to other it issues , whereas miR6250 and miR169i-3p were constitutively expressed in all it issues examined , with high abundance in roots ( miR6250 ) or leaves ( miR169i-3p ) .
The miR6250 and miR169i-3p miRNAs also exhibited distinct expression patterns in rice cultivars Minghui 86 and Nipponbare at different time points after arsenite treatment .
The predicted targets for these miRNAs included some protein kinases , DNA , or ATP-binding proteins .
Besides arsenite , the expression of targets of miR6250 and miR6254 was also up or down-regulated in response to abiotic environmental stresses , indicative of their involvement in regulation of plant adaptation .
Three types of cis-elements involved in hormone , light , and stress response were found to occur frequently in the promoter regions .
Interestingly , miR6254 was originally characterized to be an exonic miRNA located in the exon of AK101391 , supporting the notion that miRNAs may also originate from some exons in plants .
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[ Sen. 4, subscore: 2.00 ]: Quantitative real-time RT-PCR analysis revealed that miR6254 was predominantly expressed in roots relative to other it issues , whereas miR6250 and miR169i-3p were constitutively expressed in all it issues examined , with high abundance in roots ( miR6250 ) or leaves ( miR169i-3p ) .
[ Sen. 5, subscore: 1.00 ]: The miR6250 and miR169i-3p miRNAs also exhibited distinct expression patterns in rice cultivars Minghui 86 and Nipponbare at different time points after arsenite treatment .
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