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| Title: Evaluation of seven function-known candidate genes for their effects on improving drought resistance of transgenic rice under field conditions .
| | Author: Xiao BZ Chen X Xiang CB Tang N Zhang QF Xiong LZ | | Journal: Mol Plant Citation: V : 2 P : 73-83 Year: 2009 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19529831 Accession (PMID): 19529831 | | Abstract: Many stress responsive genes have been reported with an effect on improving stress resistance in model plants under greenhouse conditions .
Towards identification of genes for drought resistance breeding , seven well documented genes ( CBF3 , SOS2 , NCED2 , NPK1 , LOS5 , ZAT10 , and NHX1 ) in stress resistance were selected in this study and transformed into rice cultivar Zhonghua 11 under the control of constitutive promoter Actin1 and stress-inducible promoter of a rice HVA22 homolog , and transgenic rice were tested for drought resistance under field conditions .
A total of 1598 independent transgenic T0 plants were generated .
The percentages of single copy and expression of the transgenes were 36 . 7% and 57 . 6% , respectively .
For each gene construct , 30 T1 families with expression of transgene were selected for drought resistance testing at the reproductive stage in field , and 10 of them were tested in PVC pipes with a defined stress protocol at the same stage .
Relative yield and relative spikelet fertility were used as two major criteria to evaluate drought resistance performance because significantly decreased yield was observed in the T1 generation .
Transgenic families of eight constructs ( HVA22P : CBF3 , HVA22P : NPK1 , Actin1 : LOS5 , HVA22P : LOS5 , Actin1 : ZAT10 , HVA22P : ZAT10 , Actin1 : NHX1 , and HVA22P : NHX1 ) showed significantly higher RY than wild-type ( WT ) under both drought stress field and PVC tube conditions .
Transgenic families of 9 constructs ( HVA22P : SOS2 and CBF3 , LOS5 , ZAT10 , and NHX1 by both promoters ) showed significantly higher relative spikelet fertility than WT in the field or PVC pipes .
In the field drought resistance testing of T2 families derived from the T1 families with relatively lower yield decrease , transgenic families of seven constructs ( HVA22P : CBF3 , Actin1 : NPK1 , HVA22P : NPK1 , Actin1 : LOS5 , HVA22P : LOS5 , Actin1 : ZAT10 , and HVA22P : ZAT10 ) showed significantly higher yield per plant than WT , and families of nine constructs ( Actin1 : CBF3 , HVA22P : CBF3 , HVA22P : SOS2 , HVA22P : NPK1 , Actin1 : LOS5 , HVA22P : LOS5 , Actin1 : ZAT10 , HVA22P : ZAT10 , and Actin1 : NHX1 ) had higher spikelet fertility than WT .
In general , LOS5 and ZAT10 showed relatively better effect than the other five genes in improving drought resistance of transgenic rice under field conditions .
The results and experience obtained from this study could be a useful reference for drought resistance engineering in rice .
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[ Sen. 2, subscore: 1.00 ]: Towards identification of genes for drought resistance breeding , seven well documented genes ( CBF3 , SOS2 , NCED2 , NPK1 , LOS5 , ZAT10 , and NHX1 ) in stress resistance were selected in this study and transformed into rice cultivar Zhonghua 11 under the control of constitutive promoter Actin1 and stress-inducible promoter of a rice HVA22 homolog , and transgenic rice were tested for drought resistance under field conditions .
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