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Score: 7.00 |
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| Title: High-resolution mapping of the brown planthopper resistance gene Bph6 in rice and characterizing its resistance in the 9311 and Nipponbare near isogenic backgrounds .
| | Author: Qiu Y Guo J Jing S Zhu L He G | | Journal: Theor Appl Genet Citation: V : 121 P : 1601-11 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20680611 Accession (PMID): 20680611 | | Abstract: Brown planthopper ( Nilaparvata lugens Stal , BPH ) is one of the most destructive insect pests of rice .
Exploring resistance genes from diverse germplasms and incorporating them into cultivated varieties are critical for controlling this insect .
The rice variety Swarnalata was reported to carry a resistance gene ( designated Bph6 ) , which has not yet been assigned to a chromosome location and the resistance mechanism is still unknown .
In this study , we identified and mapped this gene using the F ( 2 ) and backcrossing populations and characterized its resistance in indica 9311 and japonica Nipponbare using near isogenic lines ( NILs ) .
In analysis of 9311/Swarnalata F ( 2 ) population , the Bph6 gene was located on the long arm of chromosome 4 between the SSR markers RM6997 and RM5742 .
The gene was further mapped precisely to a 25-kb region delimited between the STS markers Y19 and Y9 ; and the distance between these markers is 25-kb in Nipponbare genome .
The Bph6 explained 77 . 5% of the phenotypic variance of BPH resistance in F ( 2 ) population and 84 . 9% in BC ( 2 ) F ( 2 ) population .
Allele from Swarnalata significantly increased resistance to the BPH , resulted in a reduced damage score .
In characterization of Bph6-mediated resistance , the BPH insects showed significant preference between NIL-9311 and 9311 in 3 h and between NIL-NIP and Nipponbare in 120 h after release .
BPH growth and development were inhibited , and the insects survival rates were lower on Bph6-NIL plants , compared with the parents 9311 and Nipponbare .
The results indicate that the Bph6 exerted prolonged antixenotic and antibiotic effects in Bph6-NIL plants , and NIL-9311 plants showed a quicker and stronger effect toward BPH than NIL-NIP plants .
| Matching Sentences:
[ Sen. 11, subscore: 2.00 ]: The results indicate that the Bph6 exerted prolonged antixenotic and antibiotic effects in Bph6-NIL plants , and NIL-9311 plants showed a quicker and stronger effect toward BPH than NIL-NIP plants .
[ Sen. 3, subscore: 1.00 ]: The rice variety Swarnalata was reported to carry a resistance gene ( designated Bph6 ) , which has not yet been assigned to a chromosome location and the resistance mechanism is still unknown .
[ Sen. 5, subscore: 1.00 ]: In analysis of 9311/Swarnalata F ( 2 ) population , the Bph6 gene was located on the long arm of chromosome 4 between the SSR markers RM6997 and RM5742 .
[ Sen. 7, subscore: 1.00 ]: The Bph6 explained 77 . 5% of the phenotypic variance of BPH resistance in F ( 2 ) population and 84 . 9% in BC ( 2 ) F ( 2 ) population .
[ Sen. 9, subscore: 1.00 ]: In characterization of Bph6-mediated resistance , the BPH insects showed significant preference between NIL-9311 and 9311 in 3 h and between NIL-NIP and Nipponbare in 120 h after release .
[ Sen. 10, subscore: 1.00 ]: BPH growth and development were inhibited , and the insects survival rates were lower on Bph6-NIL plants , compared with the parents 9311 and Nipponbare .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Development and characterization of japonica rice lines carrying the brown planthopper-resistance genes BPH12 and BPH6 .
| | Author: Qiu Y Guo J Jing S Zhu L He G | | Journal: Theor Appl Genet Citation: V : 124 P : 485-94 Year: 2012 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub22038433 Accession (PMID): 22038433 | | Abstract: The brown planthopper ( Nilaparvata lugens Stal ; BPH ) has become a severe constraint on rice production .
Identification and pyramiding BPH-resistance genes is an economical and effective solution to increase the resistance level of rice varieties .
All the BPH-resistance genes identified to date have been from indica rice or wild species .
The BPH12 gene in the indica rice accession B14 is derived from the wild species Oryza latifolia .
Using an F ( 2 ) population from a cross between the indica cultivar 93-11 and B14 , we mapped the BPH12 gene to a 1 . 9-cM region on chromosome 4 , flanked by the markers RM16459 and RM1305 .
In this population , BPH12 appeared to be partially dominant and explained 73 . 8% of the phenotypic variance in BPH resistance .
A near-isogenic line ( NIL ) containing the BPH12 locus in the background of the susceptible japonica variety Nipponbare was developed and crossed with a NIL carrying BPH6 to generate a pyramid line ( PYL ) with both genes .
BPH insects showed significant differences in non-preference in comparisons between the lines harboring resistance genes ( NILs and PYL ) and Nipponbare .
BPH growth and development were inhibited and survival rates were lower on the NIL-BPH12 and NIL-BPH6 plants compared to the recurrent parent Nipponbare .
PYL-BPH6 + BPH12 exhibited 46 . 4 , 26 . 8 and 72 . 1% reductions in population growth rates ( PGR ) compared to NIL-BPH12 , NIL-BPH6 and Nipponbare , respectively .
Furthermore , insect survival rates were the lowest on the PYL-BPH6 + BPH12 plants .
These results demonstrated that pyramiding different BPH-resistance genes resulted in stronger antixenotic and antibiotic effects on the BPH insects .
This gene pyramiding strategy should be of great benefit for the breeding of BPH-resistant japonica rice varieties .
| Matching Sentences:
[ Sen. 7, subscore: 1.00 ]: A near-isogenic line ( NIL ) containing the BPH12 locus in the background of the susceptible japonica variety Nipponbare was developed and crossed with a NIL carrying BPH6 to generate a pyramid line ( PYL ) with both genes .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: High-resolution mapping of the brown planthopper resistance gene Bph6 in rice and characterizing its resistance in the 9311 and Nipponbare near isogenic backgrounds .
| | Author: Qiu Y Guo J Jing S Zhu L He G | | Journal: Theor Appl Genet Citation: V : 121 P : 1601-11 Year: 2010 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub20680611 Accession (PMID): 20680611 | | Abstract: Brown planthopper ( Nilaparvata lugens Stal , BPH ) is one of the most destructive insect pests of rice .
Exploring resistance genes from diverse germplasms and incorporating them into cultivated varieties are critical for controlling this insect .
The rice variety Swarnalata was reported to carry a resistance gene ( designated Bph6 ) , which has not yet been assigned to a chromosome location and the resistance mechanism is still unknown .
In this study , we identified and mapped this gene using the F ( 2 ) and backcrossing populations and characterized its resistance in indica 9311 and japonica Nipponbare using near isogenic lines ( NILs ) .
In analysis of 9311/Swarnalata F ( 2 ) population , the Bph6 gene was located on the long arm of chromosome 4 between the SSR markers RM6997 and RM5742 .
The gene was further mapped precisely to a 25-kb region delimited between the STS markers Y19 and Y9 ; and the distance between these markers is 25-kb in Nipponbare genome .
The Bph6 explained 77 . 5% of the phenotypic variance of BPH resistance in F ( 2 ) population and 84 . 9% in BC ( 2 ) F ( 2 ) population .
Allele from Swarnalata significantly increased resistance to the BPH , resulted in a reduced damage score .
In characterization of Bph6-mediated resistance , the BPH insects showed significant preference between NIL-9311 and 9311 in 3 h and between NIL-NIP and Nipponbare in 120 h after release .
BPH growth and development were inhibited , and the insects survival rates were lower on Bph6-NIL plants , compared with the parents 9311 and Nipponbare .
The results indicate that the Bph6 exerted prolonged antixenotic and antibiotic effects in Bph6-NIL plants , and NIL-9311 plants showed a quicker and stronger effect toward BPH than NIL-NIP plants .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: High-resolution mapping of the brown planthopper resistance gene Bph6 in rice and characterizing its resistance in the 9311 and Nipponbare near isogenic backgrounds .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Development and characterization of japonica rice lines carrying the brown planthopper-resistance genes BPH12 and BPH6 .
| | Author: Qiu Y Guo J Jing S Zhu L He G | | Journal: Theor Appl Genet Citation: V : 124 P : 485-94 Year: 2012 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub22038433 Accession (PMID): 22038433 | | Abstract: The brown planthopper ( Nilaparvata lugens Stal ; BPH ) has become a severe constraint on rice production .
Identification and pyramiding BPH-resistance genes is an economical and effective solution to increase the resistance level of rice varieties .
All the BPH-resistance genes identified to date have been from indica rice or wild species .
The BPH12 gene in the indica rice accession B14 is derived from the wild species Oryza latifolia .
Using an F ( 2 ) population from a cross between the indica cultivar 93-11 and B14 , we mapped the BPH12 gene to a 1 . 9-cM region on chromosome 4 , flanked by the markers RM16459 and RM1305 .
In this population , BPH12 appeared to be partially dominant and explained 73 . 8% of the phenotypic variance in BPH resistance .
A near-isogenic line ( NIL ) containing the BPH12 locus in the background of the susceptible japonica variety Nipponbare was developed and crossed with a NIL carrying BPH6 to generate a pyramid line ( PYL ) with both genes .
BPH insects showed significant differences in non-preference in comparisons between the lines harboring resistance genes ( NILs and PYL ) and Nipponbare .
BPH growth and development were inhibited and survival rates were lower on the NIL-BPH12 and NIL-BPH6 plants compared to the recurrent parent Nipponbare .
PYL-BPH6 + BPH12 exhibited 46 . 4 , 26 . 8 and 72 . 1% reductions in population growth rates ( PGR ) compared to NIL-BPH12 , NIL-BPH6 and Nipponbare , respectively .
Furthermore , insect survival rates were the lowest on the PYL-BPH6 + BPH12 plants .
These results demonstrated that pyramiding different BPH-resistance genes resulted in stronger antixenotic and antibiotic effects on the BPH insects .
This gene pyramiding strategy should be of great benefit for the breeding of BPH-resistant japonica rice varieties .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Development and characterization of japonica rice lines carrying the brown planthopper-resistance genes BPH12 and BPH6 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
