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| Title: Cloning and characterization of the gene for a phloem-specific glutathione S-transferase from rice leaves .
| | Author: Fukuda A Okada Y Suzui N Fujiwara T Yoneyama T Hayashi H | | Journal: Citation: V : 120 ( 4 ) P : 595-602 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15032821 Accession (PMID): 15032821 | | Abstract: The N-terminal amino-acid sequence was determined for a major rice phloem protein with a molecular mass of 31 kDa , named RPP31 .
The corresponding full-length rice EST-clone was cloned based on the amino acid sequence .
The predicted total amino-acid sequence of RPP31 shared high similarity with plant glutathione S-transferases ( GSTs ) .
Recombinant RPP31 produced in Escherichia coli and rice phloem sap showed GST activity .
Immunocytological analysis indicated that RPP31 is localized in the phloem region of leaves .
In mature leaves , the signal was restricted to sieve element-companion cell complexes , and was stronger in sieve elements than in companion cells .
Although some plant GSTs are known to be induced by xenobiotics , the amount of RPP31 was not affected by treatments with an herbicide , pretilachlor , and/or its safener , fenclorim .
These results suggest that RPP31 is an active GST restricted to the phloem region of normal rice leaves .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The N-terminal amino-acid sequence was determined for a major rice phloem protein with a molecular mass of 31 kDa , named RPP31 .
[ Sen. 3, subscore: 1.00 ]: The predicted total amino-acid sequence of RPP31 shared high similarity with plant glutathione S-transferases ( GSTs ) .
[ Sen. 4, subscore: 1.00 ]: Recombinant RPP31 produced in Escherichia coli and rice phloem sap showed GST activity .
[ Sen. 5, subscore: 1.00 ]: Immunocytological analysis indicated that RPP31 is localized in the phloem region of leaves .
[ Sen. 7, subscore: 1.00 ]: Although some plant GSTs are known to be induced by xenobiotics , the amount of RPP31 was not affected by treatments with an herbicide , pretilachlor , and/or its safener , fenclorim .
[ Sen. 8, subscore: 1.00 ]: These results suggest that RPP31 is an active GST restricted to the phloem region of normal rice leaves .
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