%D 2001 %0 ARTICLE %T Detection and characterization of glutathione S-transferase activity in rice EF-1betabetagamma and EF-1gamma expressed in Escherichia coli . %J Biochem . Biophys . Res . Commun . %V 288 ( 3 ) %P 509-14 %A Kobayashi S %A Kidou S %A Ejiri S %M pub11676472 %X Plant elongation factor EF-1 consists of four subunits ( EF-1alphabetabetagamma ) . EF-1alpha . GTP catalyses the binding of aminoacyl-tRNA to the ribosome . EF-1beta and EF-1beta catalyze the GDP/GTP exchange on EF-1alpha . GDP . However , the function of EF-1gamma , a subunit detected in eukaryotes , but not in prokaryotes remained unknown . This report demonstrates that rice EF-1betabetagamma and recombinant EF-1gamma possess glutathione S-transferase ( GST ) activity . The EF-1betabetagamma or EF-1gamma-dependent GST activity is about one-fiftieth of the rice GST activity . The Km values of EF-1betabetagamma , EF-1gamma , and rice GST for glutathione and 1-chloro-2 , 4-dinitrobenzene are of about the same order . Although recombinant EF-1gamma is heat labile , active EF-1gamma was obtained by purifying it in the presence of 20% glycerol .