%D 2000 %0 ARTICLE %T Floral spray transformation can efficiently generate Arabidopsis transgenic plants . %J Transgenic Res . %V 9 ( 6 ) %P 471-6 %A Chung MH %A Chen MK %A Pan SM %M pub11206976 %X In this study , floral spray and floral dip were used to replace the vacuum step in the Agrobacterium-mediated transformation of a superoxide dismutase ( SOD ) gene into Arabidopsis . The transgene was constructed by using a CaMV 35S promoter to drive a rice cytosolic CuZnSOD coding sequence in Arabidopsis . The transgene construct was developed in binary vectors and mobilized into Agrobacterium . When Arabidopsis plants started to initiate flower buds , the primary inflorescence shoots were removed and then transformed by floral spray or floral dip . More than 300 transgenic plants were generated to assess the feasibility of floral spray used in the in planta transformation . The result indicates that the floral spray method of Agrobacterium can achieve rates of in planta transformation comparable to the vacuum-infiltration and floral dip methods . The floral spray method opens up the possibility of in planta transformation of plant species which are too large for dipping or vacuum infiltration .