%D 2000 %0 ARTICLE %T Laboratory evaluation of chemical-biological control of the rice weevil ( Sitophilus oryzae L ) in stored grains . %J %V 37 ( 1 ) %P 77-84 %A Dal Bello %A G Padin %A S Lpez %A Lastra C %A Fabrizio M %M pub11124371 %X The virulence of ten different fungal isolates of : Beauveria bassiana , Metarhizium anisopliae , Verticillium lecanii and Paecilomyces farinosus to the rice weevil Sitophilus oryzae was tested . A fungal mix of the most efficient isolates , B bassiana ARSEF 5500+M anisopliae ARSEF 2974 , which caused the highest mortality , was assayed in combination with fenitrothion at a concentration lower ( 3 ppm ) than the normal 6 ppm . Fungal inoculation of insects was done by spraying conidial suspensions of each fungus on wheat . Insecticide formulations were added by spraying wheat . Treated and untreated insects were incubated on durum wheat . Insects were kept in a climatized chamber for 30 days . Observations were performed at 7 , 14 and 30 days to record insect mortality . Highly significant differences were demonstrated for B bassiana 5500 and 5501 and for M anisopliae 2974 . The level of mortality produced by treatments was : 6 ppm insecticide=97 . 50% , B bassiana ARSEF 5500+M anisopliae ARSEF 2974+3 ppm insecticide=74 . 17% , B bassiana ARSEF 5500+Manisopliae ARSEF 2974=50% and 3 ppm insecticide=37 . 50% . There was a statistically significant difference ( p<0 . 05 ) among treatments .